Previous studies from our group have demonstrated that glucose solution can induce optical clearing enhancement of esophageal tissues with optical coherence tomography (OCT). The aims of this study were to evaluate the optical clearing effects of ultrasound-mediated optical clearing agents (OCAs) and to find more effective methods to distinguish human normal esophageal tissues (NE) and cancerous esophageal tissues (CE). Here we used the OCT technique to investigate the optical attenuation of NE and CE in vitro after treatment with 30% glycerol alone and glycerol combined with ultrasound, respectively. Experimental results showed that the averaged attenuation coefficient of CE was significantly ...

The potential of OCT applied for early breast cancer detection attracted significant efforts. The permeability coefficients and the percentages of OCT signal enhancement for normal and cancerous breast tissues have been investigated from the experiments of 20% glucose, 40% glucose, and 20% mannitol in vitro. Obtained results indicate that the permeability coefficient in breast cancer tissue is prominently larger than that in normal breast tissue, while the optical clearing effect is just the reverse to that for each agent. The results suggest that OCT has the ability to distinguish cancer tissue from different aspect.

The potential of OCT applied to noninvasive blood glucose monitoring has attracted significant efforts. In this work we investigated the feasibility of OCT in monitoring blood glucose during oral intake of different sugars in humans. Five groups of experiments were performed, in which different sugars were used. The OCT signal slope (OCTSS) changed with variation of blood glucose concentration (BGC). A good correlation between OCTSS and BGC was observed in these experiments. The averaged correlation coefficients R between OCTSS and BGC are 0.900, 0.836, 0.895 and 0.884, corresponding to oral administration of glucose, fructose, sucrose and ...

The objective of this study was to evaluate the effects of ultrasound-mediated analyte diffusion on permeability of normal, benign, and cancerous human lung tissue in vitro and to find more effective sonophoretic (SP) delivery in combination with the optical clearing agents (OCAs) method to distinguish normal and diseased lung tissues. The permeability coefficients of SP in combination with OCAs diffusion in lung tissue were measured with Fourier-domain optical coherence tomography (FD-OCT). 30% glucose and SP with a frequency of 1 MHz and an intensity of 0.80     W / cm 2 over a 3 cm probe was simultaneously applied for 15 ...

Oncogenesis and metastasis of tumor are difficult to detect during the clinic therapy. To explore the optical properties of tumorigenesis and metastasis in nasopharyngeal carcinoma (NPC), we assessed the NPC cell lines 5-8F and 6-10B by optical coherence tomography (OCT): first, the OCT images of the two different types of cell pellets were captured. Second, by fitting Beer’s law to the averaged A-scans in these OCT datasets, the attenuation coefficients ( μ t ) of the cells were extracted. The median attenuation coefficients (interquartile range (IQR)) of 5-8F and 6-10B were 6.79 mm −1 (IQR 6.52 to 7.23 mm ...

Hyperosmotic agents have shown great potential in tissue optical clearing. However, the low efficiency of the permeation in biological tissues seriously restricts its application in reality. The synergy of sonophoresis as a penetration enhancer and hyperosmotic agents, 20% glucose (G) and 20% mannitol (M), in optical clearing has been investigated by analyzing the variation of the attenuation coefficients and the permeability coefficients. In the sonophoresis experiments, ultrasound (US) was applied for 10 min before applying hyperosmotic agents. Along with the administration of hyperosmotic agents, the samples were monitored with optical coherence tomography (OCT) functional imaging for the next 2 h ...

Recently, the capability of optical coherence tomography (OCT) has been demonstrated for noninvasive blood glucose monitoring. In this work, we investigate the administration of chemical agents onto human skin tissue to increase the transparency of the surface of the skin, as a means of improving the capability of OCT imaging for clinically relevant applications. Eight groups of experiments were proposed, in which different optical clearing agents (OCA) were used. The results indicate that, when properly used, some OCAs perform well in promoting the capability of OCT for noninvasive blood glucose monitoring. Among the four kinds of OCA we used, 50 ...