Purpose . To evaluate Fourier-domain optical coherence tomography (FD-OCT) as an adjunct to traditional slit lamp examination of donor corneas with suspected Anterior Stromal Opacities. Methods . Seven corneas suspected of having anterior stromal opacities by slit lamp examination were evaluated with FD-OCT. Each cornea was evaluated to confirm the presence of opacity and, if present, the depth of opacity was measured. Results . The opacity depth ranged from 82  μ m to 624  μ m. The initial slit lamp impressions of five of the seven corneas were confirmed by OCT. In two corneas, the OCT findings were different from the initial slit lamp impressions ...

Most of the optical coherence tomographic (OCT) systems for high resolution imaging of biological specimens are based on refractive type microscope objectives, which are optimized for specific wave length of the optical source. In this study, we present the feasibility of using commercially available reflective type objective for high sensitive and high resolution structural and functional imaging of cochlear microstructures of an excised guinea pig through intact temporal bone. Unlike conventional refractive type microscopic objective, reflective objective are free from chromatic aberrations due to their all-reflecting nature and can support a broadband of spectrum with very high light collection efficiency.

Purpose: To evaluate the Descemet stripping automated endothelial keratoplasty (DSAEK) graft deturgescence in preservation medium after microkeratome cut using Fourier domain optical coherence tomography. Methods: The central and peripheral thickness of DSAEK grafts was measured by Fourier domain optical coherence tomography immediately after microkeratome cuts and 1, 2, 3, and 4 hours afterward. All measurements were taken when the grafts were stored in 4[degrees]C preservation medium. The hourly change in central graft thickness and graft shape (peripheral graft thicknes - central graft thickness) was calculated and tracked over time. Results: Five DSAEK grafts were measured. The average central graft ...

A major reason we can perceive faint sounds and communicate in noisy environments is that the outer hair cells of the organ of Corti enhance the sound-evoked motions inside the cochlea. To understand how the organ of Corti works, we have built and tested the phase-sensitive Fourier domain optical coherence tomography (PSFDOCT) system. This system has key advantages over our previous time domain OCT system [1]. The PSFDOCT system has better signal to noise and simultaneously acquires vibration data from all points along the optical-axis [2]. Feasibility of this system to measure in vitro cochlear vibrations in the apex was ...

Champalimaud Vision Award 2012 Highlights Carmen A. Puliafito, Dean of the Keck School of Medicine of USC, is honored along with James Fujimoto, Eric A. Swanson, David Huang and Joel S. Schuman with the prestigious 2012 Champalimaud Vision Award on Sept. 14 in Lisbon, Portugal. The team received the award for the development of Optical Coherence Tomography (OCT), a technology that has revolutionized the diagnosis and treatment of patients with blinding diseases such as macular degeneration, diabetic retinopathy and glaucoma. n

Purpose . To assess optical coherence tomography (OCT) for guiding design and fit of a prosthetic device for corneal disease. Methods . A prototype time domain OCT scanner was used to image the anterior segment of patients fitted with large diameter (18.5–20 mm) prosthetic devices for corneal disease. OCT images were processed and analyzed to characterize corneal diameter, corneal sagittal height, scleral sagittal height, scleral toricity, and alignment of device. Within-subject variance of OCT-measured parameters was evaluated. OCT-measured parameters were compared with device parameters for each eye fitted. OCT image correspondence with ocular alignment and clinical fit was assessed. Results ...