Purpose. To evaluate the inter-visit reproducibility of spectral-domain optical coherence tomography (SD-OCT) measurement of the macular retinal nerve fiber layer thickness (mRNFLT), combined ganglion cell layer and inner plexiform layer (GCL+IPL) thickness, and ganglion cell complex (GCC) thicknesses (sum of mRNFLT and GCL+IPL thicknesses) compared with that of circumpapillary RNFLT (cpRNFLT) and the effect of ocular rotation on reproducibility. Methods. SD-OCT imaging was performed twice on different days in one eye of 58 normal subjects and 73 glaucoma patients. The reproducibility was evaluated for the entire 4.8 mm x 4.8 mm macular area and sub-areas (upper ...

Purpose. To determine the retinal features associated with a dissociated optic nerve fiber layer (DONFL) appearance after successful macular hole surgery with internal limiting membrane (ILM) peeling. Methods. Forty-seven eyes of 47 patients that underwent pars plana vitrectomy with ILM peeling were examined by spectral-domain optical coherence tomography (SD-OCT). Results. A DONFL appearance was found in 31 (66.0%) eyes on color fundus photography. The postoperative best-corrected visual acuity and its improvements were not significantly different between eyes with and without this appearance. The SD-OCT images showed small inner retinal defects corresponding to each arcuate stria in all eyes with ...

Purpose To study peripapillary morphologic changes in highly myopic eyes using swept-source optical coherence tomography at a longer wavelength. Design Prospective cross-sectional study. Methods Peripapillary regions of 196 eyes of 107 patients with high myopia (refractive error, <−8.0 diopters or axial length, >26.0 mm) were analyzed quantitatively and qualitatively with an swept-source optical coherence tomography prototype system that uses a tunable laser light source operated at a 100,000-Hz A-scan repetition rate in the 1-μm wavelength region. The visual field was evaluated by standard automated perimetry. Area of peripapillary atrophy β and presence of scleral protrusion temporal to ...

Purpose To detect pathologic changes in retinal nerve fiber bundles in glaucomatous eyes seen on images obtained by adaptive optics (AO) scanning laser ophthalmoscopy (AO SLO). Design Prospective cross-sectional study. Methods Twenty-eight eyes of 28 patients with open-angle glaucoma and 21 normal eyes of 21 volunteer subjects underwent a full ophthalmologic examination, visual field testing using a Humphrey Field Analyzer, fundus photography, red-free SLO imaging, spectral-domain optical coherence tomography, and imaging with an original prototype AO SLO system. Results The AO SLO images showed many hyperreflective bundles suggesting nerve fiber bundles. In glaucomatous eyes, the nerve fiber bundles were narrower ...

Purpose: To test the glaucoma-discriminating ability of a new method for detecting local ganglion cell loss using spectral-domain optical coherence tomography (SD-OCT). Methods: This study included 58 eyes from glaucomatous patients and 48 healthy eyes from volunteers. The combined thickness of the ganglion cell layer and inner plexus layer (GCIPL) was measured on a macular cube scan (200 × 200) in Cirrus HD-OCT. GCIPL thicknesses on 360 spokes extending from the inner to the outer radius of a macular elliptical annulus were calculated. The lowest value (minimum GCIPL) was compared to the average GCIPL thickness within the elliptical annulus in the ...

Purpose: To determine whether measurement of ganglion cell complex (GCC) thickness over a wide area (8-mm diameter) can improve the glaucoma-discriminating ability of spectral-domain optical coherence tomography (SD-OCT) compared to that in the standard macular area (6-mm diameter). Methods: Ninety-three subjects were enrolled, including 46 healthy eyes of 46 volunteers and 47 eyes of 47 glaucoma patients (23 eyes with preperimetric glaucoma [PPG] and 24 eyes with early glaucoma [EG]). All patients underwent SD-OCT raster scanning over a 9 mm × 9 mm square area centered on the fovea. Areas under the receiver operating characteristic curves (AROCs) were compared between wide ...

Background Recently, a transgenic rabbit with rhodopsin Pro 347 Leu mutation was generated as a model of retinitis pigmentosa (RP), which is characterized by a gradual loss of vision due to photoreceptor degeneration. The purpose of the current study is to noninvasively visualize and assess time-dependent changes in the retinal structures of a rabbit model of retinal degeneration by using speckle noise-reduced spectral-domain optical coherence tomography (SD-OCT). Methodology/Principal Findings Wild type (WT) and RP rabbits (aged 4–20 weeks) were investigated using SD-OCT. The total retinal thickness in RP rabbits decreased with age. The thickness of the outer nuclear ...

Purpose: To compare automated assessment of macular drusen delineated by our originally-developed algorithm on 3-dimensional (3D) spectral-domain optical coherence tomography (SD-OCT) with the assessment by certificated graders on color fundus photographs in non-neovascular age-related macular degeneration (AMD). Design: Prospective case series. Methods: Automated assessment of macular drusen was performed using raster scan by 3D OCT-1000 (Topcon) in 18 eyes with non-neovascular AMD with at least one large druse (≥125μm) and predominantly soft indistinct drusen. We defined drusen as the regions that have the distance between the RPE and calculated Bruch's membrane lines > predefined threshold distances. We assessed the agreement ...