We report the development of a combined dual-modal photoacoustic and optical coherence tomography (PA-OCT) system using a single near-infrared (NIR) supercontinuum laser source which can provide both optical absorption and scattering contrasts simultaneously. By using a small sized pulsed Nd:YAG microchip laser and a photonic crystal fiber, we fabricated a pulsed broadband supercontinuum source from 600 to 1700 nm. Under the same optical hardware system, intrinsically registered PA and OCT images are acquired in a single scanning. In order to demonstrate feasibility of our system, we successfully acquired the PA and OCT images of black and white hairs images ...

Abstract  In this study, we distinguished Cucumber green mottle mosaic virus (CGMMV) infected seeds from healthy seeds using optical coherence tomography (OCT). Two dimensional OCT images and stereo micrographs revealed that the infected seeds had narrow gap between the seed coat and endosperm that were not present in the healthy seeds. Three dimensional OCT images confirmed that the narrow gaps were present in the inner structure of overall the infected seeds. A-scan analysis was also performed to calculate the distance from the seed coat to the endosperm. The results revealed a difference in the width of the gap of about ...