We demonstrate the use of a modified Rayleigh–Lamb frequency equation in conjunction with noncontact optical coherence elastography to quantify the viscoelastic properties of the cornea. Phase velocities of air-pulse-induced elastic waves were extracted by spectral analysis and used for calculating the Young’s moduli of the samples using the Rayleigh–Lamb frequency equation (RLFE). Validation experiments were performed on 2% agar phantoms ( n = 3 ) and then applied to porcine corneas ( n = 3 ) in situ . The Young’s moduli of the porcine corneas were estimated to be ∼ 60     kPa with a shear viscosity ∼ 0.33     Pa ⋅ s . The results demonstrate ...

The cardiovascular system is the first functional organ system to develop within the mammalian embryo. During the early stages of cardiovascular development, the heart and blood vessels undergo rapid growth and remodeling required for embryo viability, proper morphogenesis, and the function of all organ systems. Live imaging of these dynamic events in early mouse embryos is critical to understanding when and how these morphological changes occur during normal development and how mutations and pharmacological agents affect cardiovascular structure and function in vivo. The use of optical coherence tomography (OCT) allows for rapid, three-dimensional structural and functional imaging of mouse embryos ...

Current technique for tumor resection requires biopsy of the tumor region and histological confirmation before the surgeon can be certain that the entire tumor has been resected. This confirmation process is time consuming both for the surgeon and the patient and also requires sacrifice of healthy tissue, motivating the development of novel technologies which can enable real-time detection of tumor-healthy tissue boundary for faster and more efficient surgeries. In this study, the potential of combining structural information from optical coherence tomography (OCT) and molecular information from line-scan Raman microscopy (LSRM) for such an application is presented. The results show a ...