1. Gereon Hüttmann

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    1. Mentioned In 71 Articles

    2. Coregistered Spectral Optical Coherence Tomography and Two-Photon Microscopy for Multimodal NearInstantaneous Deep-Tissue Imaging

      Coregistered Spectral Optical Coherence Tomography and Two-Photon Microscopy for Multimodal NearInstantaneous Deep-Tissue Imaging
      Two-photon microscopy (2PM) has brought unique insight into the mechanisms underlying immune system dynamics and function since it enables monitoring of cellular motility and communication in complex systems within their genuine environmentthe living organism. However, use of 2PM in clinical settings is limited. In contrast, optical coherence tomography (OCT), a noninvasive label-free diagnostic imaging method, which allows monitoring morphologic changes of large tissue regions in vivo, has found broad application ...
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      Mentions: Gereon Hüttmann
    3. Dynamic contrast in scanning microscopic OCT

      Dynamic contrast in scanning microscopic OCT
      While optical coherence tomography (OCT) provides a resolution down to 1 micrometer it has difficulties to visualize cellular structures due to a lack of scattering contrast. By evaluating signal fluctuations, a significant contrast enhancement was demonstrated using time-domain full-field OCT (FF-OCT), which makes cellular and subcellular structures visible. The putative cause of the dynamic OCT signal is ATP-dependent motion of cellular structures in a sub-micrometer range, which provides histology-like contrast ...
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    4. Intravital microscopic optical coherence tomography imaging to assess mucus mobilizing interventions for muco-obstructive lung disease in mice

      Intravital microscopic optical coherence tomography imaging to assess mucus mobilizing interventions for muco-obstructive lung disease in mice
      Airway mucus obstruction is a hallmark of chronic lung diseases such as cystic fibrosis, asthma and COPD, and the development of more effective mucus mobilizing therapies remains an important unmet need for patients with these muco-obstructive lung diseases. However, methods for sensitive visualization and quantitative assessment of immediate effects of therapeutic interventions on mucus clearance in vivo are lacking. In this study, we determined if newly developed high-speed microscopic optical ...
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    5. Segmentation of Retinal Low-Cost Optical Coherence Tomography Images using Deep Learning

      Segmentation of Retinal Low-Cost Optical Coherence Tomography Images using Deep Learning
      The treatment of age-related macular degeneration (AMD) requires continuous eye exams using optical coherence tomography (OCT). The need for treatment is determined by the presence or change of disease-specific OCTbased biomarkers. Therefore, the monitoring frequency has a significant influence on the success of AMD therapy. However, the monitoring frequency of current treatment schemes is not individually adapted to the patient and therefore often insufficient. While a higher monitoring frequency would ...
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    6. Measurement and visualization of stimulus-evoked tissue dynamics in mouse barrel cortex using phase-sensitive optical coherence tomography

      Measurement and visualization of stimulus-evoked tissue dynamics in mouse barrel cortex using phase-sensitive optical coherence tomography
      ... Imaging,” Front. Neurosci. 12, 269 (2018). [Crossref] H. Spahr, C. Pfäffle, S. Burhan, L. Kutzner, F. Hilge, G. Hüttmann, and D. Hillmann, “Phase-sensitive interferometry of decorrelated speckle patterns,” Sci. Rep. 9(1...
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    7. Capillary Refill—The Key to Assessing Dermal Capillary Capacity and Pathology in Optical Coherence Tomography Angiography

      Capillary Refill—The Key to Assessing Dermal Capillary Capacity and Pathology in Optical Coherence Tomography Angiography
      Background/Objectives: Standard optical coherence tomography angiography (OCTA) has been limited to imaging blood vessels actively undergoing perfusion, providing a temporary picture of surface microvasculature. Capillary perfusion in the skin is dynamic and changes in response to the surrounding tissue's respiratory, nutritional, and thermoregulatory needs. Hence, OCTA often represents a given perfusion state without depicting the actual extent of the vascular network. Here we present a method for obtaining ...
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    8. Investigation of cell dynamics in 3D cell spheroids and cell interaction with 3D printed scaffolds by mOCT

      Investigation of cell dynamics in 3D cell spheroids and cell interaction with 3D printed scaffolds by mOCT
      Optical coherence tomography is a non-invasive and label-free imaging modality based on the detection of backscattered light in samples. As microscopic OCT (mOCT) combines high axial and lateral resolution, an investigation of biological and printed samples at subcellular level is feasible. The additional excellent depth resolution enables mOCT to be a suitable quality control of 3D printed samples. The use of speckle variance adds information about cell viability. Here we ...
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    9. Computational adaptive optics for optical coherence tomography using multiple randomized subaperture correlations

      Computational adaptive optics for optical coherence tomography using multiple randomized subaperture correlations
      Computational adaptive optics (CAO) is emerging as a viable alternative to hardware-based adaptive opticsin particular when applied to optical coherence tomography of the retina. For this technique, algorithms are required that detect wavefront errors precisely and quickly. Here we propose an extension of the frequently used subaperture image correlation. By applying this algorithm iteratively and, more importantly, comparing each subaperture not to the central subaperture but to several randomly selected ...
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    10. Segmentation of mouse skin layers in optical coherence tomography image data using deep convolutional neural networks

      Segmentation of mouse skin layers in optical coherence tomography image data using deep convolutional neural networks
      Optical coherence tomography (OCT) enables the non-invasive acquisition of high-resolution three-dimensional cross-sectional images at micrometer scale and is mainly used in the field of ophthalmology for diagnosis as well as monitoring of eye diseases. Also in other areas, such as dermatology, OCT is already well established. Due to its non-invasive nature, OCT is also employed for research studies involving animal models. Manual evaluation of OCT images of animal models is ...
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    11. Optimization-based vessel segmentation pipeline for robust quantification of capillary networks in skin with optical coherence tomography angiography

      Optimization-based vessel segmentation pipeline for robust quantification of capillary networks in skin with optical coherence tomography angiography
      Optical coherence tomography angiography (OCTA) provides in-vivo images of microvascular perfusion in high resolution. For its application to basic and clinical research, an automatic and robust quantification of the capillary architecture is mandatory. Only this makes it possible to reliably analyze large amounts of image data, to establish biomarkers, and to monitor disease developments. However, due to its optical properties, OCTA images of skin often suffer from a poor signal-to-noise ...
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    12. Artifacts in speckle tracking and multi-aperture Doppler OCT imaging of lateral motion

      Artifacts in speckle tracking and multi-aperture Doppler OCT imaging of lateral motion
      In optical coherence tomography (OCT), lateral motion is determined either by speckle tracking or by multi-aperture Doppler OCT. Here we show that both methods may provide incorrect results because, outside the focal plane, non-uniform axial motion is misinterpreted as lateral motion. First, we demonstrate the existence of this artifact by means of a simulation for speckle tracking. Then the physical origin of the artifact and its mathematical relation to defocus ...
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    13. Method for filtering reflexes in full-field setups for ophthalmologic imaging by separated illumination and detection apertures

      Method for filtering reflexes in full-field setups for ophthalmologic imaging by separated illumination and detection apertures
      A parallel detecting optical coherence tomography (OCT) setup and method, in which the light paths of the illumination of the sample and of the detection of the backscattered light do not use the same apertures. The separation of illumination and detection apertures filters these disturbing reflexes from the backscattered light of the sample and significantly increases image quality.
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    14. Simple approach for aberration-corrected OCT imaging of the human retina

      Simple approach for aberration-corrected OCT imaging of the human retina
      Aberration-corrected imaging of human photoreceptor cells, whether hardware or software based, presently requires a complex and expensive setup. Here we use a simple and inexpensive off-axis full-field time-domain optical coherence tomography (OCT) approach to acquire volumetric data of an in vivo human retina. Full volumetric data are recorded in 1.3 s. After computationally correcting for aberrations, single photoreceptor cells were visualized. In addition, the numerical correction of ametropia is ...
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    15. High-speed fiber scanning endoscope for volumetric multi-megahertz optical coherence tomography

      High-speed fiber scanning endoscope for volumetric multi-megahertz optical coherence tomography
      We present a forward-viewing fiber scanning endoscope (FSE) for high-speed volumetric optical coherence tomography (OCT). The reduction in size of the probe was achieved by substituting the focusing optics by an all-fiber-based imaging system which consists of a combination of scanning single-mode fibers, a glass spacer, made from a step-index multi-mode fiber, and a gradient-index fiber. A lateral resolution of 11 m was achieved at a working distance of 1 ...
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    16. Interferometric detection of 3D motion using computational subapertures in optical coherence tomography

      Interferometric detection of 3D motion using computational subapertures in optical coherence tomography
      Doppler optical coherence tomography (OCT) quantifies axial motion with high precision, whereas lateral motion cannot be detected by a mere evaluation of phase changes. This problem was solved by the introduction of three-beam Doppler OCT, which, however, entails a high experimental effort. Here, we present the numerical analogue to this experimental approach. Phase-stable complex-valued OCT datasets, recorded with full-field swept-source OCT, are filtered in the Fourier domain to limit imaging ...
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    17. 1-15 of 71 1 2 3 4 5 »
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  2. About Gereon Hüttmann

    Gereon Hüttmann

    Gereon Hüttmann is with the Institute for Biomedical Optics at the University of Lübeck.