The study of intact, living cells using non-invasive optical spectroscopic methods offers the opportunity to assess cellular structure and organization in a way that is not possible with commonly used cell biology imaging techniques. We have developed a novel spectroscopic technique for diagnosing disease at the cellular level based on using low-coherence interferometry (LCI) to detect the angular distribution of scattered light. Angle-resolved LCI (a/LCI) combines the ability of LCI to isolate scattering from sub-surface tissue layers with the ability of light scattering spectroscopy to obtain structural information on sub-wavelength scales. In application to examining cellular structure, a/LCI ...

Aim . To investigate the utility of fundus autofluorescence (FAF) and optical coherence tomography (OCT) in the evaluation of cystoid macular edema (CME) following cataract surgery. Materials and Methods . Forty eyes of 29 patients undergone phacoemulsification, with posterior chamber intraocular lens implantation surgery. Central macular thickness (CMT) of the patients was evaluated using OCT and FAF preoperatively and postoperative 1st, 30th, 60th, 90th, and 180th days. Results . CME was detected in three eyes (7.5%) of two patients using OCT. Hyperautofluorescence (HAF) was detected in two of these three eyes and resolved with treatment. In the remaining 37 eyes without CME ...

Duke University Received a 2013 NIH Grant for $410,399 for Novel Determination of Microbicide PK in Women's Re...

Background/aims Spectral-domain (SD)- Optical Coherence Tomography (OCT) can track eye movements, has faster acquisition time and higher resolution than time-domain(TD)-OCT. The aim of the study was to assess the utility of SD-OCT in paediatric glaucoma and determine its agreement with TD-OCT. Methods Children who had SD-OCT(Spectralis, Heidelberg-Engineering,Germany) were retrospectively and prospectively identified from Duke paediatric glaucoma clinic. The peripapillary retinal nerve fibre layer (RNFL) and macular thickness and volume (MV) were compared amongst four groups: normal eyes, eyes with physiologic cupping (C:D >0.5 and <0.8, IOP <21), mild glaucomatous eyes (C:D ...

Purpose: To correlate spectral domain optical coherence tomography (SD OCT)-determined morphologic alterations in eyes with geographic atrophy because of age-related macular degeneration with lesion size, enlargement rate, and the presence of multifocal patches of atrophy. Methods: Forty-three eyes of 43 patients with age-related macular degeneration-associated geographic atrophy were visualized by SD OCT and fundus autofluorescence imaging. The baseline area of geographic atrophy and enlargement rates over at least 24 weeks were calculated from the fundus autofluorescence images. The mean and median follow-up times were 47.4 and 48 weeks, respectively. Morphologic alterations were evaluated in the baseline SD OCT ...

Purpose: The authors have recently developed a high-resolution microscope-integrated spectral domain optical coherence tomography (MIOCT) device designed to enable OCT acquisition simultaneous with surgical maneuvers. The purpose of this report is to describe translation of this device from preclinical testing into human intraoperative imaging. Methods: Before human imaging, surgical conditions were fully simulated for extensive preclinical MIOCT evaluation in a custom model eye system. Microscope-integrated spectral domain OCT images were then acquired in normal human volunteers and during vitreoretinal surgery in patients who consented to participate in a prospective institutional review board-approved study. Microscope-integrated spectral domain OCT images were obtained ...

Methods of reducing motion artifacts in Optical Coherence Tomography (OCT) include scanning a sample with a scan pattern to acquire OCT data at a plurality of data locations. The data locations are distributed in the scan pattern across the sample such that at least some spatially adjacent data locations are acquired non-sequentially in time. A profile of the sample corresponding to a sample surface or an aspect of an internal structure of the sample is estimated responsive the OCT data.

The Duke University Received a $64,511 2013 NIH Grant for Comparison of AMD Treatment Trials-OCT Reading Center. T...

A surgical microscope assembly includes a microscope main objective and microscope imaging optics. The microscope main objective and microscope imaging optics define a viewing beam path that passes from a sample through the microscope main objective and the microscope imaging optics. The assembly includes an optical coherence tomography (OCT) unit having an illumination beam and a collection beam and a beamsplitter between the microscope main objective and the microscope imaging optics. The beamsplitter is configured to direct a portion of light from the microscope main objective to the microscope imaging optics and to direct another portion of light from the ...

Optical Coherence Tomography (OCT) allows insights in pediatric ophthalmology. In order to interpret OCT findings, one must be familiar with its basic concepts as well as well as with findings of recent studies.