University of Illinois Urbana-Champaign Receives NIH Grant for Intraoperative Label-Free Optical Molecular Imaging of Breast Tumor Margins
Bone marrow (BM)-derived stem and immune cells play critical roles in maintaining the health, regeneration, and repair of many tissues. Given their important functions in tissue regeneration and therapy, tracking the dynamic behaviors of BM-derived cells has been a long-standing research goal of both biologists and engineers. Because of the complex cellular-level processes involved, real-time imaging technologies that have sufficient spatial and temporal resolution to visualize them are needed. In addition, in order to track cellular dynamics, special attention is needed to account for changes in the microenvironment where the cells reside, for example, tissue contraction, stretching, development, etc ...
Feature Of The Week 5/12/13: University of Illinois at Urbana-Champaign Reports on Real-Time In Vivo Computed Optical Interferometric Tomography
Real-time, high-resolution tomography over an extended field-of-view in scattering tissues remains an elusive goal for biomedical imaging. The apparent trade-off between the depth-of-field and lateral resolution in optical imaging degrades the image resolution outside the confocal region in OCT. Although a number of approaches have been utilized to overcome these limitations, many of these techniques are plagued by either high computational complexity or complicated optical setups. ISAM is a computational technique that can yield spatially invariant transverse resolution by resampling the Fourier space of the data. However, a full 3D ISAM reconstruction requires extensive manipulation of volumetric datasets, imposing significant ...
Optical coherence tomography (OCT) has long been used as a diagnostic tool in the field of ophthalmology. The ability to observe microstructural changes in the tissues of the eye has proved very effective in diagnosing ocular disease. However, this technology has yet to be introduced into the primary care office, where indications of disease are first encountered. We have developed a portable, handheld imaging probe for use in the primary care setting and evaluated its tissue site accessibility, ability to observe diseased tissue, and screening capabilities in in vivo human patients, particularly for pathologies related to the eye, ear and ...
High-resolution real-time tomography of scattering tissues is important for many areas of medicine and biology 1 , 2 , 3 , 4 , 5 , 6 . However, the compromise between transverse resolution and depth-of-field, in addition to low sensitivity deep in tissue, continues to impede progress towards cellular-level volumetric tomography. Computed imaging has the potential to solve these long-standing limitations. Interferometric synthetic aperture microscopy 7 , 8 , 9 is a computed imaging technique enabling high-resolution volumetric tomography with spatially invariant resolution. However, its potential for clinical diagnostics remains largely untapped because full volume reconstructions required lengthy post-processing, and the phase-stability requirements have been difficult to satisfy ...
Investigation of bacterial biofilm in the human middle ear using optical coherence tomography and acoustic measurements
Roles of ionic strength and biofilm roughness on adhesion kinetics of Escherichia coli onto groundwater biofilm grown on PVC surfaces
Magnetomotive optical coherence tomography (MM-OCT) is a functional extension of OCT which utilizes magnetically responsive materials that are modulated by an external magnetic field for contrast enhancement and for elastography to assess the structural and viscoelastic properties of the surrounding tissues. Traditionally, magnetomotive contrast relies on the interaction between the displacement of magnetic particles induced by an external magnetic field and the micro-environmental restoring (elastic) force acting on the particles. When the restoring force from a sample containing magnetic particles is weak or non-existent, the MM-OCT signal-to-noise ratio (SNR) can degrade significantly. We have developed a novel solenoid configuration to ...
Intraoperative assessment of tumor margins with a new optical imaging technology: A multi-center, randomized, blinded clinical trial
Background: Partial mastectomy is the most commonly performed procedure for invasive breast cancer and is associated with a reexcision rate commonly ranging from 20% to 40% in the literature. This high rate of reexcision is associated with significant additional cost (estimated over $4,000 per reexcision) and lower quality outcomes. Optical coherence tomography (OCT) is a high-resolution imaging technology that images tissue structure with micron-scale resolution – on the same scale as histopathology. It is similar to ultrasound except it uses near infra-red light waves instead of sound waves to create detailed images several millimeters deep into tissue. Although widely used ...
Feature Of The Week 12/9/12: University of Illinois at Urbana Champaign Researchers Extend Astronomical Guide-Star Techniques to Computational OCT Imaging
Researchers at the University of Illinois at Urbana-Champaign have a long history of novel work in the field of OCT and related fields. This includes the very promising area of computational adaptive optics and related methods that offer the promise of dramatically improved images in a wide variety of settings by performing post-acquisition processing of magnitude and phase data. Below is a summary of some of their recent work. Aberrations degrade resolution, contrast, and signal-to-noise ratio in optical microscopy. In optical coherence tomography (OCT), aberrations limit the resolution of diagnostic features, notably in retinal OCT. With optical coherence microscopy, which ...
We present a method for the numerical correction of optical aberrations based on indirect sensing of the scattered wavefront from point-like scatterers (“guide stars”) within a three-dimensional broadband interferometric tomogram. This method enables the correction of high-order monochromatic and chromatic aberrations utilizing guide stars that are revealed after numerical compensation of defocus and low-order aberrations of the optical system. Guide-star-based aberration correction in a silicone phantom with sparse sub-resolution-sized scatterers demonstrates improvement of resolution and signal-to-noise ratio over a large isotome. Results in highly scattering muscle tissue showed improved resolution of fine structure over an extended volume. Guide-star-based computational adaptive ...
Modern caries treatment concepts like caries management by risk assessment—CAMBRA—entail diagnosing early caries lesions in a precavitated stage to make it possible to reverse the caries process with remineralization and bacteria reduction efforts. Newer, sensitive caries diagnostic tools can serve not only for early detection but also for monitoring of caries lesions to confirm the success of prevention and remineralization efforts. This article describes light-based caries diagnostic tools, with emphasis on fluorescence-based techniques, and compares the most common available fluorescence-based tools with a standardized visual caries inspection system—the International Caries Detection and Assessment System (ICDAS II). Fluorescence ...
About Stephen A. Boppart
Stephen A. Boppart is a professor in the Biophotonics Imaging Laboratory at the University of Illinois Urbana Champaign. Dr. Boppart received his Ph.D. from the Massachusetts Institute of Technology in 1998, followed by a M.D. from Harvard Medical School in 2000. Currently Prof. Boppart is a full-time faculty member in the Beckman Institute Nanoelectronics and Biophotonics Group as well as an associate professor in the UIUC Department of Electrical and Computer Engineering and the Bioengineering Department. In January 2007, he was named the Founding Director of the Mills Breast Cancer Institute at Carle Foundation Hospital. Dr. Boppart is also a Clinical Research Physician in the UI College of Medicine-Urbana. His fields of professional interest include optical imaging (specifically in Optical Coherence Tomography) and biophotonics in medicine and biology.
Jim's innovation, scholarly activities, professional service, entrepreneurial efforts, and impact on the field of biomedical optics typifies the spirit of this award, and reflects the seminal changes that Britton Chance made during his lifetime...Few researchers in the world today have had such a profound impact as a result of their technological work that has literally changed our field, changed the way we practice medicine, and directly improved the lives of perhaps hundreds of thousands of patients (considering ophthalmology and cardiology).In Fujimoto Honored with Britton Chance Biomedical Optics Award
We know that antibiotics don’t always work well if you have a biofilm, because the bacteria protect themselves and become resistant...In the presence of a chronic ear infection that has a biofilm, the bacteria may not respond to the usual antibiotics, and you need to stop them. But without being able to detect the biofilm, we have no idea whether or not it’s responding to treatment.In Nowhere to hide: New device sees bacteria behind the eardrum
I think it's going to dramatically change things...What we hope is that diagnosis is going to get shifted closer and closer to the point of care...We’re developing techniques to get at molecular changes..So much of medicine and pathology are based on structural changes. If we think of a pathologist looking at a slide, he or she looks at the cells and tissue structures. A radiologist will look at how organs and these anatomical structures are arranged...But with a lot of these techniques, we can get the molecular changes where disease starts. So a pathologist that has molecular information, not just structural, will perhaps catch disease earlier. The same is true for Rohit’s work and Gabi’s work.In Positive Results: A New Era for Medical Diagnostics - News from UIUC
It's the same challenge, but instead of imaging through the atmosphere, we're imaging through tissue, and instead of imaging a star, we're imaging a cell.In Computing the best high-resolution 3-D tissue images - News from Beckman Institute at UIUC
The effectiveness is striking...Because of the aberrations of the human eye, when you look at the retina without adaptive optics you just see variations of light and dark areas that represent the rods and cones. But when you use adaptive optics, you see the rods and cones as distinct objects...are working to compute the best image possible.In Computing the best high-resolution 3-D tissue images - News from Beckman Institute at UIUC
to emphasize the role of medical imaging and how this technology has enabled us to look into the body at many different size scales, how imaging has enabled us to diagnose disease, and how imaging has made a difference in our healthcare...Federal dollars have been used to fund technology that’s going to change and improve health care. They already have. In the area of optics and high-resolution optical imaging, there’s going to be better healthcare, economic development with new companies, new jobs, and new areas of research to investigate...We can now do real-time microscopic imaging in the operating room without waiting for pathology.In Boppart Presents at Congressional Briefing
In the end, I expect the cost of this system will be slightly more than what it replaces, but with significantly more capabilities, I do expect the cost of this system to continue to fall as more systems are developed and demand increases...This will be a boon for poorer hospitals and Third World or developing countries...It is essentially a portable imaging system with digital data that can be sent via cell-phone networks for analysis by experts in larger cities/hospitals.In Scientists awarded grant to continue developing optical device for medical exams
The result of this – if successful, could really reduce our health care costs and streamline our delivery of health care.In NIH Awards Stephen Boppart $5M For A Bioengineering Research Partnership to Develop Handheld Optical Imaging Technology
We are trying to build a small, handheld unit that has multiple tips...What’s collected is 3D digital data that can image several millimeters into tissue and at micron-scale resolution...The primary care physician is the best person to screen the general population for disease...“We think that it’s going to completely change the way we treat ear infections.In NIH Awards Stephen Boppart $5M For A Bioengineering Research Partnership to Develop Handheld Optical Imaging Technology
The diagnosis is made based on very subjective interpretation – how the cells are laid out, the structure, the morphology...This is what we call the gold standard for diagnosis. We want to make the process of medical diagnostics more quantitative and more rapid.In New imaging technique accurately finds cancer cells, fast