1. 1-11 of 11
    1. Spectral Domain Optical Coherence Tomography: An In Vivo Imaging Protocol for Assessing Retinal Morphology in Adult Zebrafish

      Spectral Domain Optical Coherence Tomography: An In Vivo Imaging Protocol for Assessing Retinal Morphology in Adult Zebrafish

      The present study outlines a protocol for examining retinal structure in zebrafish, a popular model organism for ocular studies, using spectral domain optical coherence tomography (SD-OCT). We demonstrate how this live imaging modality can be used to obtain high quality images of several retinal features, including the optic nerve, retinal vasculature, and the cone photoreceptor mosaic. Retinal histology sections were obtained from imaged fish for comparison with SD-OCT cross-sectional B-scans. Voronoi domain analysis was used to assess cone photoreceptor packing regularity at 3, 6, and 12 months. SD-OCT is an effective in vivo technique for studying the adult zebrafish retina ...

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    2. A Pilot Study of In Vivo Confocal Laser Endomicroscopy of Upper Tract Urothelial Carcinoma

      A Pilot Study of In Vivo Confocal Laser Endomicroscopy of Upper Tract Urothelial Carcinoma

      Purpose: Tissue diagnosis of upper tract urothelial carcinoma (UTUC) is limited by variance in tumor sampling by standard ureteroscopic biopsy. Optical imaging technologies can potentially improve UTUC diagnosis, surveillance, and endoscopic treatment. We previously demonstrated in vivo optical biopsy of urothelial carcinoma of the bladder using confocal laser endomicroscopy (CLE). In this study, we evaluated a new 0.85-mm imaging probe in the upper urinary tract and demonstrated feasibility and compatibility with standard ureteroscopes to achieve in vivo optical biopsy of UTUC. Patients, Materials and Methods: Fourteen patients scheduled for ureteroscopy of suspected upper tract lesions or surveillance of UTUC ...

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    3. A Simple Optical Coherence Tomography Quantification Method for Choroidal Neovascularization

      A Simple Optical Coherence Tomography Quantification Method for Choroidal Neovascularization

      Purpose: Therapeutic efficacy is routinely assessed by measurement of lesion size using flatmounted choroids and confocal microscopy in the laser-induced choroidal neovascularization (L-CNV) rodent model. We investigated whether optical coherence tomography (OCT) quantification, using an ellipsoid volume measurement, was comparable to standard ex vivo evaluation methods for this model and whether this approach could be used to monitor treatment-related lesion changes. Methods: Bruch's membrane was ruptured by argon laser in the dilated eyes of C57BL/6J mice, followed by intravitreal injections of anti-VEGF 164 or vehicle, or no injection. In vivo OCT images were acquired using Micron III or ...

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    4. Dynamic assessment of the endothelialization of tissue-engineered blood vessels using an OCT catheter-based fluorescence imaging system

      Dynamic assessment of the endothelialization of tissue-engineered blood vessels using an OCT catheter-based fluorescence imaging system

      Background: Lumen endothelialization of bioengineered vascular scaffolds is essential to maintain small-diameter graft patency and prevent thrombosis post implantation. Unfortunately non-destructive imaging methods to visualize this dynamic process are lacking, thus slowing development and clinical translation of these potential tissue-engineering approaches. To meet this need, a fluorescence imaging system utilizing a commercial optical coherence tomography (OCT) catheter was designed to visualize graft endothelialization. Methods: DragonFly C7 intravascular OCT catheter was used as a channel for delivery and collection of excitation and emission spectra. Poly-DL-lactide (PDLLA) electrospun scaffolds were seeded with endothelial cells. Seeded cells were exposed to Calcein AM prior ...

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    5. Optical diagnostics for Upper Urinary Tract Urothelial cancer: technology, thresholds and clinical applications

      Optical diagnostics for Upper Urinary Tract Urothelial cancer: technology, thresholds and clinical applications

      Purpose: Developments in optical diagnostics have potential for less invasive diagnose of upper urinary tract urothelial carcinoma (UUT-UC). This systematic review provides an overview of technology, applications and limitations of recently developed optical diagnostics in the upper urinary tract and outlines their potential for future clinical applications. In addition, current evidence was evaluated. Literature search: A PubMed literature search was performed and papers on Narrow Band Imaging (NBI), Photodynamic Diagnosis (PDD), Storz Professional Imaging Enhancement System (SPIES), Optical Coherence Tomography (OCT) and Confocal Laser Endomicroscopy (CLE) regarding UUT-UC were reviewed for data extraction. Study quality was reviewed according to QUADAS ...

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    6. Application of Optical Coherence Tomography to Identify Pulp Exposure During Access Cavity Preparation Using an Er:YAG Laser

      Application of Optical Coherence Tomography to Identify Pulp Exposure During Access Cavity Preparation Using an Er:YAG Laser

      Objective : The study aimed to evaluate the ability of optical coherence tomography (OCT) to guide and identify pulp exposure using an erbium: yttrium–aluminum–garnet (Er:YAG) laser. Background data: The Er:YAG laser has been proven to be effective in ablating dental hard tissue and offers advantages, as there is none of the vibration and noise you get with conventional methods, but it has limitations in relation to the tactile feedback that would aid in identification of entry into the pulp chamber. Based on depth-resolved optical reflectivity, OCT technology has been developed to provide high-resolution, cross-sectional images of the ...

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    7. Topographic Optic Disc Changes After Successful Trabeculectomy Evaluated Using Spectral Domain Optical Coherence Tomography

      Topographic Optic Disc Changes After Successful Trabeculectomy Evaluated Using Spectral Domain Optical Coherence Tomography

      Purpose: To investigate topographic optic disc changes after surgical intraocular pressure (IOP) reduction in adults with open angle glaucoma (OAG). Methods: Optic discs of patients with advanced primary open angle or exfoliation glaucoma were imaged using optical coherence tomography within 1 week before trabeculectomy . Patients were rescanned 1 week, 1 month, and 1 year after surgery. Maximum cup depth (MCD), and average cup depth (ACD) were calculated. Results: Twenty-two eyes from 20 patients (age, 70.5±10.6 years; average mean defect of visual field, −15.0±9.8 dB) were followed up for 1 year. The IOP decreased from ...

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    8. Optical Coherence Tomography as an Auxiliary Tool for the Screening of Radiation-Related Caries

      Optical Coherence Tomography as an Auxiliary Tool for the Screening of Radiation-Related Caries

      Objective: The aim of this study was to evaluate the morphological alterations of radiation-related caries using optical coherence tomography. Methods: Thirty-six extracted teeth from 11 patients who had undergone radiotherapy were sectioned in the sagittal axis in the center of the carious lesion, and 100 μm thick sections were obtained from each specimen. One sample from each tooth was investigated by an optical coherence tomography (OCT) system, and the results were compared with histological images from polarized light microscopy. Results: In OCT dentin caries images, the demineralized area appeared as a white region, whereas the translucent zone appeared as a ...

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    9. Detection of Root Surface Fractures with Swept-Source Optical Coherence Tomography (SS-OCT)

      Detection of Root Surface Fractures with Swept-Source Optical Coherence Tomography (SS-OCT)

      Objective: The purpose of this study was to compare optical coherence tomography (OCT) with the existing technologies, to assess its accuracy and utility in detecting vertical root fractures of extracted human teeth. Background data: The detection of root fractures in teeth that have undergone root canal treatment is challenging because of the great difficulty in differentiating these fractures from morphologic or radiographic anomalies. OCT methods are based on depth-resolved optical reflectivity and have been developed to reduce the invasiveness and radiation exposure inherent to other techniques. Methods: Twelve extracted human mandibular teeth (totaling 25 roots) that were free of caries ...

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    10. Investigation of Neovascularization in Three-Dimensional Porous Scaffolds In Vivo by a Combination of Multiscale Photoacoustic Microscopy and Optical Coherence Tomography

      Investigation of Neovascularization in Three-Dimensional Porous Scaffolds In Vivo by a Combination of Multiscale Photoacoustic Microscopy and Optical Coherence Tomography

      It is a grand challenge to visualize and assess in vivo neovascularization in a three-dimensional (3D) scaffold non-invasively, together with high spatial resolution and deep penetration depth. Here we used multiscale photoacoustic microscopy (PAM), including acoustic-resolution PAM (AR-PAM) and optical-resolution PAM (OR-PAM), to chronically monitor neovascularization in an inverse opal scaffold implanted in a mouse model up to six weeks by taking advantage of the optical absorption contrast intrinsic to hemoglobin molecules in red blood cells. By combining with optical coherence tomography (OCT) based on optical scattering contrast, we also demonstrated the capability to simultaneously image and analyze the vasculature ...

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    11. Monitoring sinew contraction during formation of tissue-engineered fibrin-based ligament constructs

      Monitoring sinew contraction during formation of tissue-engineered fibrin-based ligament constructs

      The ability to study the gross morphological changes occurring during tissue formation is vital to producing tissue-engineered structures of clinically relevant dimensions in vitro. Here, we have used non-destructive methods of digital imaging and optical coherence tomography to monitor the early-stage formation and subsequent maturation of fibrin-based tissue-engineered ligament constructs. In addition, the effect of supplementation with essential promoters of collagen synthesis, ascorbic acid (AA) and proline (P), have been assessed. Contraction of the cell-seeded fibrin gel occurs unevenly within the first 5 days of culture around two fixed anchor points before forming a longitudinal ligament-like construct. AA+P supplementation ...

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    1-11 of 11
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