For many clinical applications, such as dermatology, optical coherence tomography (OCT) suffers from limited penetration depth due primarily to the highly scattering nature of biological tissues. Here, we present a novel implementation of dual-axis optical coherence tomography (DA-OCT) that offers improved depth penetration in skin imaging at 1.3 µm compared to conventional OCT. Several unique aspects of DA-OCT are examined here, including the requirements for scattering properties to realize the improvement and the limited depth of focus (DOF) inherent to the technique. To overcome this limitation, our approach uses a tunable lens to coordinate focal plane selection with image ...

We present a computationally highly efficient full-wave spectral model of OCT-scan formation with the following features: allowance of arbitrary phase-amplitude profile of illuminating beams; absence of paraxial approximation; utilization of broadly used approximation of ballistic scattering by discrete scatterers without limitations on their density/location and scattering strength. The model can easily incorporate the wave decay, dispersion, measurement noises with given signal-to-noise ratios and arbitrary inter-scan displacements of scatterers. We illustrate several of such abilities, including comparative simulations of OCT-scans for Bessel versus Gaussian beams, presence of arbitrary aberrations at the tissue boundary and various scatterer motions. The model flexibility ...

Spectral-estimation OCT (SE-OCT) is a computational method to enhance the axial resolution beyond the traditional bandwidth limit. However, it has not yet been used widely due to its high computational load, dependency on user-optimized parameters, and inaccuracy in intensity reconstruction. In this study, we implement SE-OCT using a fast implementation of the iterative adaptive approach (IAA). This non-parametric spectral estimation method is optimized for use on OCT data. Both in simulations and experiments we show an axial resolution improvement with a factor between 2 and 10 compared to standard discrete Fourier transform. Contrary to parametric methods, IAA gives consistent peak ...

We present a robust fiber-based setup for Bessel-like beam extended depth-of-focus Fourier-domain optical coherence microscopy, where the Bessel-like beam is generated in a higher order mode fiber module. In this module a stable guided LP 02 core mode is selectively excited by a long period grating written in the higher order mode fiber. Imaging performance of this system in terms of lateral resolution and depth of focus was analyzed using samples of suspended microbeads and compared to the case where illumination is provided by the fundamental LP 01 mode of a single mode fiber. Illumination with the LP 02 mode ...

All-normal dispersion supercontinuum (ANDi SC) generation in a lead-bismuth-gallate glass solid-core photonic crystal fiber (PCF) with cladding air-holes infiltrated with carbon tetrachloride (CCl 4 ) is experimentally investigated and numerically verified. The liquid infiltration results in additional degrees of freedom that are complimentary to conventional dispersion engineering techniques and that allow the design of soft-glass ANDi fibers with an exceptionally flat near-zero dispersion profile. The unique combination of high nonlinearity and low normal dispersion enables the generation of a coherent, low-noise SC covering 0.93–2.5 µm requiring only 12.5 kW of pump peak power delivered by a standard ...

Endoscopic airway optical coherence tomography (OCT) is an advanced imaging modality capable of capturing the internal anatomy and geometry of the airway. Due to fiber-optic catheter bending and friction, the rotation speed of the endoscopic probe is usually non-uniform: at each B-scan image, the initial rotation angle of the probe is easily misaligned with that of the previous slices. During the pullback operation, this initial rotation angle error (IRAE) will be accumulated and will result in distortion and deformation of the reconstructed 3D airway structure. Previous attempts to correct this error were mainly manual corrections, which are time-consuming and suffered ...

To realize multimodal hemodynamic imaging, pulse photothermal optical coherence tomography (P-PTOCT) is proposed in this Letter to solve the separation problem of photothermal phase and Doppler phase, which is difficult to solve in traditional PTOCT. This technique can obtain blood flow distribution, light absorption distribution, and concentration images simultaneously. Based on the difference between pulse photothermal phase and Doppler phase, we propose an even number differential demodulation algorithm that can separate the photothermal phase and Doppler phase from the same scanning data set. The separated photothermal phase can characterize the trend of drug concentration, which provides the possibility for quantitative ...

Photothermal optical coherence tomography (PT-OCT) is an emerging extension of OCT, which forms images based on both scattering and absorption of light. The speed of PT-OCT, however, has been limited by the necessity for lock-in detection with extensive temporal sampling of the sample’s PT response. Here, we demonstrate transient-mode PT-OCT (TM-PT-OCT), which increases the effective A-line rate by orders of magnitude from 10–100 Hz to 1.5–7.5 kHz, by interrogating the sample’s transient thermal response to a single diode laser pulse. Functional imaging of moving samples with TM-PT-OCT at video rate is also presented. This ...

Optical coherence tomography (OCT) and OCT angiography (OCT-A) may benefit the screening of diabetic retinopathy (DR). This study investigated the effect of laterally subsampling OCT/OCT-A en face scans by up to a factor of 8 when using deep neural networks for automated referable DR classification. There was no significant difference in the classification performance across all evaluation metrics when subsampling up to a factor of 3, and only minimal differences up to a factor of 8. Our findings suggest that OCT/OCT-A can reduce the number of samples (and hence the acquisition time) for a volume for a given ...

Optical coherence tomography (OCT) is used for diagnosis of esophageal diseases such as Barrett's esophagus. Given the large volume of OCT data acquired, automated analysis is needed. Here we propose a bilateral connectivity-based neural network for in vivo human esophageal OCT layer segmentation. Our method, connectivity-based CE-Net (Bicon-CE), defines layer segmentation as a combination of pixel connectivity modeling and pixel-wise tissue classification. Bicon-CE outperformed other widely used neural networks and reduced common topological prediction issues in tissues from healthy patients and from patients with Barrett's esophagus. This is the first end-to-end learning method developed for automatic segmentation of ...

Inner surface defects of inertial confinement fusion (ICF) capsule are a key factor leading to ignition failure; however, there are still no effective and non-destructive detection methods available. To solve this problem, we propose the first interferometric microscope with confocal focusing (CFIM). CFIM first uses confocal technology to achieve accurate axial positioning of both capsule and the camera, thereby ensuring that the inner surface of the capsule is precisely and clearly imaged at the camera. Then, phase-shifting interferometry based on a short-coherence source and a spherical reference is applied to obtain inner defects result from null inner surface interferograms. In ...

We report an Optical Differentiation Wavefront Sensor based on a telephoto lens system and binary pixelated filters. It provides a five-fold reduction in the system length compared to a 4 f system with identical effective focal length. Measurements of phase plates with this system are compared to measurements performed with a commercial low-coherence interferometer. The telephoto-lens-based system can measure wavefronts with accuracy better than λ/ 10 Root Mean Squared (RMS) at λ =633 nm. Experimental investigation shows that the system has a high tolerance to components alignment errors.

In this study, the principle of ‘optical palpation’ was applied to a compression optical coherence elastography (OCE) method using spectral domain optical coherence tomography (OCT). Optical palpation utilizes a compliant transparent material of known mechanical properties, which acts as a stress sensor, in order to derive the mechanical properties of a sample material under examination. This technique was applied to determine the mechanical properties of soft contact lenses, with one lens being used as the compliant stress sensor and the other as the sample under investigation to extract the mechanical properties. This compliant stress sensor allowed for the stress of ...

Optical sectioning is instrumental for the observation of extended biological samples. It allows the observation of only a slice of the sample while rejecting contributions from out of focus depths. The acquisition of the whole volume then requires an axial displacement of the sample or the focus. To satisfy Nyquist sampling, this axial displacement has to be equal to half the axial resolution. As lateral and axial resolutions are coupled by the numerical aperture of the microscope objective in most imaging techniques, high-resolution imaging of a volume is a time-consuming task, especially caused by the slow axial scanning. Here, we ...

Achieving high resolution in optical coherence tomography typically requires the continuous extension of the spectral bandwidth of the light source. This work demonstrates an alternative approach: combining two discrete spectral windows located in the visible spectrum with a trained conditional generative adversarial network (cGAN) to reconstruct a high-resolution image equivalent to that generated using a continuous spectral band. The cGAN was trained using OCT image pairs acquired with the continuous and discontinuous visible range spectra to learn the relation between low- and high-resolution data. The reconstruction performance was tested using 6000 B-scans of a layered phantom, micro-beads and ex-vivo mouse ...

A non-invasive diagnostic tool to assess remodeling of the lung airways caused by disease is currently missing in the clinic. Measuring key features such as airway smooth muscle (ASM) thickness would increase the ability to improve diagnosis and enable treatment evaluation. In this research, polarization-sensitive optical coherence tomography (PS-OCT) has been used to image a total of 24 airways from two healthy lungs and four end-stage diseased lungs ex vivo , including fibrotic sarcoidosis, chronic obstructive pulmonary disease (COPD), fibrotic hypersensitivity pneumonitis, and cystic fibrosis. In the diseased lungs, except COPD, the amount of measured airway smooth muscle was increased. In ...

High lateral resolution ( ∼ 5 µ m ∼5µm ) optical coherence tomography (OCT) that employs a variable cross-cylinder (VCC) to compensate for astigmatism is presented for visualizing minute structures of the human retina. The VCC and its sensorless optimization process enable ocular astigmatism correction of up to − 5.0 −5.0 diopter within a few seconds. VCC correction has been proven to increase the signal-to-noise ratio and lateral resolution using a model eye. This process is also validated using the human eye by visualizing the capillary network and human cone mosaic. The proposed method is applicable to existing OCT, making high lateral ...

Chicks are an excellent model for studying myopia. To study the change of the ocular structures in chicks, ultrasound is mostly used. However, it suffers from limited spatial resolution. In this study, we investigated the axial length (AL) and the thickness of different ocular structures in chicks’ eye undergoing visually induced changes using a swept-source optical coherence tomography (SS-OCT) system in vivo . Two groups of chicks wore a translucent plastic goggle (n = 6) over the right eye to induce form-deprivation myopia. Following 12 days of form deprivation, goggles were removed in one group of chicks (n = 3), and they were ...

We present a completely label-free three-dimensional (3D) optical coherence tomography (OCT)-based tissue dynamics imaging method for visualization and quantification of the metabolic and necrotic activities of tumor spheroid. Our method is based on a custom 3D scanning protocol that is designed to capture volumetric tissue dynamics tomography images only in a few tens of seconds. The method was applied to the evaluation of a tumor spheroid. The time-course viability alteration and anti-cancer drug response of the spheroid were visualized qualitatively and analyzed quantitatively. The similarity between the OCT-based dynamics images and fluorescence microscope images was also demonstrated.

Ocular aberrometry is an essential technique in vision science and ophthalmology. We demonstrate how a phase-sensitive single mode fiber-based swept source optical coherence tomography (SS-OCT) setup can be employed for quantitative ocular aberrometry with digital adaptive optics (DAO). The system records the volumetric point spread function at the retina in a de-scanning geometry using a guide star pencil beam. Succeeding test-retest repeatability assessment with defocus and astigmatism analysis on a model eye within ± 3 D dynamic range, the feasibility of technique is demonstrated in-vivo at a B-scan rate of >1 kHz in comparison with a commercially available aberrometer.

We present a low-coherence interferometric imaging system designed for 3-dimensional (3-D) imaging of a macroscopic object through a narrow passage. Our system is equipped with a probe-type port composed of a bundle fiber for imaging and a separate multimode optical fiber for illumination. To eliminate the need for mechanical depth scanning, we employ a spatial frequency multiplexing method by installing a 2-D diffraction grating and an echelon in the reference arm. This configuration generates multiple reference beams, all having different path lengths and propagation directions, which facilitates the encoding of different depth information in a single interferogram. We demonstrate the ...

Full-field swept-source optical coherence tomography (FF-SS-OCT) is an emerging technology with potential applications in ophthalmic imaging, microscopy, metrology, and other domains. Here we demonstrate a novel method of multiplexing FF-SS-OCT signals using carrier modulation (CM). The principle of CM could be used to inspect various properties of the scattered light, e.g. its spectrum, polarization, Doppler shift, or distribution in the pupil. The last of these will be explored in this work, where CM was used to acquire images passing through two different optical pupils. The two pupils contained semicircular optical windows with perpendicular orientations, with each window permitting measurement ...