The purpose of this study is to compare the predictive accuracy of intraocular lens (IOL) calculations made with partial coherence interferometry (PCI, IOLMaster, version 5) and swept-source optical coherence tomography (SS-OCT, Argos). Axial length (AL), mean keratometry value (K), and anterior chamber depth (ACD) were obtained using PCI and SS-OCT optical biometers. Intraocular lens (IOL) power calculations were made using the Barret-Universal II, Haigis, Hoffer Q, SRK/T, and T2 formulas and compared the predictive accuracy between biometers. In 153 eyes (153 patients), axial length measurements made with PCI (24.65 ± 2.35 mm) and SS-OCT (24.62 ± 2.29 ...

As a summer intern at the private foundation Brain and Spine Institute (ICM) in Paris, Olivier Thouvenin helped develop an imaging technique for monitoring the neural circuitry in zebrafish larvae. But the method lacked the spatial resolution to tease apart connections between neurons at the single-cell level, which frustrated the young biophysics master’s student. So for his PhD at the Langevin Institute — two kilometres away on the edge of Paris’s famed Botanical Gardens — Thouvenin worked on improving an existing high-resolution tissue-imaging tool called full-field optical coherence tomography (OCT). He added a dynamic time element to the otherwise static ...

Optical coherence tomography angiography (OCT-A) is a non-invasive alternative to fluorescein angiography that allows for the study of the retinal and choroidal vasculatures. In this retrospective cohort study of 28 patients with retinitis pigmentosa (RP), we used OCT-A to quantify changes in perfusion density, foveal avascular zone (FAZ) area, and choriocapillaris blood flow over time and correlated these variables with ellipsoid zone (EZ) line width and best-corrected visual acuity (BCVA). Perfusion density decreased by 2.42 ± 0.62% per year at the superior capillary plexus (SCP) (P = 0.001) and 2.41 ± 0.76% per year at the deep capillary ...

We evaluated automated OCT-derived drusen volume measures in a population-based study (n = 4,512) aged ≥40 years, and its correlation with conventional color fundus photographs (CFP)-derived early AMD features. Participants had protocol-based assessment to capture medical and ocular history, genotyping for SNPs in CFH , ARMS2 , and CETP , CFP-based AMD grading and automated drusen volume based on SD-OCT using built-in software (Cirrus OCT advanced RPE analysis software). Significantly fewer eyes with early AMD features (drusen, hyperpigmentation, soft or reticular drusen) had drusen volume = 0 mm 3 (p < 0.001). In eyes with drusen volume > 0 mm 3 , increasing AMD severity ...

The quality of the scan image is important in microvascular circulation analysis using optical coherence tomography (OCTA). We aimed to investigate the effect of signal strength (SS) on OCTA metrics and minimum SS level that could be considered optimal. Macular 6 × 6 mm angiography images were acquired, and all subjects were divided into four groups according to the SS (SS 7, SS 8, SS 9, and SS 10) of the OCTA image. Vessel density (VD), perfusion density (PD), and foveal avascular zone (FAZ) metrics of the superficial capillary plexus were compared. In total, 446 eyes from young healthy subjects were ...

Latex, an aqueous dispersion of sub-micron polymer particles, is widely used as polymer binder in waterborne coatings and adhesives. Drying of a latex is inhomogeneous, during which the spatial distribution of particles is non-uniform and changes with time, usually resulting in a compromise of the integrity of a dried film. To study drying inhomogeneity of latex, we developed a system integrating optical coherence tomography (OCT) with gravimetric and video analysis (OCT-Gravimetry-Video method) to non-destructively monitor the drying process of non-film-forming latexes consisting of hard polystyrene spheres over time. OCT structural and speckle images of the latex’s internal structure show ...

To study types and thickness of interdigitation zone band in adult Chinese subjects, we conducted a cross-sectional study. The population-based Beijing Eye Study 2011 included 3468 individuals with a mean age of 64.6 ± 9.8 years. 263 people (263eyes) with a mean age of 64.8 years were randomly selected cases without macular diseases included in the study. A detailed ophthalmic examination was performed including SD-OCT for measurement of the thickness of interdigitation zone band. There are two types of interdigitation zone band; the type1 which can distinguish RPE–BM complex in 170 eyes; and the Type 2 which ...

The volume and complexity of diagnostic imaging is increasing at a pace faster than the availability of human expertise to interpret it. Artificial intelligence has shown great promise in classifying two-dimensional photographs of some common diseases and typically relies on databases of millions of annotated images. Until now, the challenge of reaching the performance of expert clinicians in a real-world clinical pathway with three-dimensional diagnostic scans has remained unsolved. Here, we apply a novel deep learning architecture to a clinically heterogeneous set of three-dimensional optical coherence tomography scans from patients referred to a major eye hospital. We demonstrate performance in ...

Corneal neovascularization (CoNV) could be treated by novel anti-angiogenic therapies, though reliable and objective imaging tools to evaluate corneal vasculature and treatment efficacy is still lacking. Optical coherence tomography angiography (OCTA) –currently designed as a retinal vascular imaging system— has been recently adapted for anterior-segment and showed good potential for successful imaging of CoNV. However, further development requires an animal model where parameters can be studied more carefully with histological comparison. Our study evaluated the OCTA in suture-induced CoNV in a rabbit model compared to indocyanine green angiography (ICGA) and slit-lamp photography (SLP). Overall vessel density measurements from OCTA showed ...

This pilot study uses a micro-optical coherence tomography (micro-OCT) system with ~1 μm axial resolution specifically to image the cornea and corneal scars in vivo . We used an established murine corneal scar model by irregular phototherapeutic keratectomy in ten C57BL/6 mice, with serial imaging using the micro-OCT and compared to anterior segment (AS-OCT) (RTvue, Optovue, Fremont, CA) before and after scar induction. Main outcome was agreement between the AS-OCT and micro-OCT using Bland-Altman plots (95% limits of agreement, LoA).We analysed 10 control eyes and 10 eyes with corneal scars and found that there was good agreement between AS-OCT ...

The hippocampus is associated with memory and navigation, and the rodent hippocampus provides a useful model system for studying neurophysiology such as neural plasticity. Vascular changes at this site are closely related to brain diseases, such as Alzheimer’s disease, dementia, and epilepsy. Vascular imaging around the hippocampus in mice may help to further elucidate the mechanisms underlying these diseases. Optical coherence tomography angiography (OCTA) is an emerging technology that can provide label-free blood flow information. As the hippocampus is a deep structure in the mouse brain, direct in vivo visualisation of the vascular network using OCTA and other microscopic ...

Acquisition of high-resolution images from within internal organs using endoscopic optical imaging has numerous clinical applications. However, difficulties associated with optical aberrations and the trade-off between transverse resolution and depth of focus significantly limit the scope of applications. Here, we integrate a metalens, with the ability to modify the phase of incident light at subwavelength level, into the design of an endoscopic optical coherence tomography catheter (termed nano-optic endoscope) to achieve near diffraction-limited imaging through negating non-chromatic aberrations. Remarkably, the tailored chromatic dispersion of the metalens in the context of spectral interferometry is utilized to maintain high-resolution imaging beyond the ...

Although ganglion cell inner plexiform layer (GC-IPL) analysis in the patients with high myopia is useful, there have been few reports to analyze of the reliability for long-term measured GC-IPL thickness. We aimed to analyze the long-term reproducibility of thickness measurements of the GC-IPL using spectral-domain optical coherence tomography (SD-OCT) in patients with high myopia and identify factors that affect such reproducibility. 99 eyes from 99 patients with high myopia without any other ophthalmc disorder such as glaucoma or retinal diseases were included. Two serial SD-OCT (Cirrus-HD) macular scans taken at ≧ 1 year intervals were analyzed. The intraclass correlation coefficient ...

The purpose of this study was to identify the normative values of ganglion cell-inner plexiform layer (GCIPL) thickness in healthy Korean children using swept-source optical coherence tomography (SS-OCT) and to investigate the correlations of age, refractive error, axial length (AL), retinal nerve fiber layer (RNFL) thickness and cup-to-disc (C/D) ratio with GCIPL thickness. Children aged between 3 and 17 who had visited our pediatric ophthalmology clinic were enrolled. Each subject underwent full ophthalmic examinations including RNFL thickness, C/D ratio and GCIPL thickness measurement by SS-OCT as well as AL measurement by partial-coherence interferometry. A total of 254 eyes ...

The high signal throughput of Fourier transform spectroscopy has proved a boon to overcoming the difficulties of otherwise overwhelming detector noise in chemical infrared spectroscopy and near-infrared Raman spectroscopy, and is desirable for in situ measurement. Here we introduce a stationary channel dispersed Fourier transform (CDFT) spectrometer that efficiently utilizes two-dimensional imagers to obtain high-resolution input spectra in low-pixel-density imagers. By angularly dispersing a vertical one-dimensional interference pattern along a horizontal axis, an array of bandlimited interferogram channels is measured with reduced sampling constraints in each channel. CDFT spectrometers can use existing imagers to measure spectra with 0.0025 cm ...

Purpose To determine the effects of age on perifoveal cone density in healthy subjects using adaptive optics. Methods Healthy subjects of various ages were imaged using an adaptive optics retinal camera (RTX-1 ® Imagine Eyes, Orsay, France). All patients underwent a comprehensive ophthalmologic examination and retinal imaging using spectral-domain optical coherence tomography (Spectralis ® , Heidelberg Engineering, Heidelberg, Germany). Cone density together with cone spacing and cone mosaic packing were measured in the nasal and temporal area 450 µm from the fovea. A multivariate analysis was performed to determine which of the following parameters were related to a decrease in cone density: age ...

Accurate alignment of an intraocular lens (IOL) is indispensable for achieving accurate postoperative refractive outcomes. Thus, we evaluated decentration and tilt of single- and three-piece IOLs, as well as anterior chamber depth (ACD), at 3 hours, 24 hours, 2 weeks, and 4 weeks after cataract surgery, using swept-source anterior segment optical coherence tomography. There was no significant difference in postoperative visual acuity between eyes with single- or three-piece IOLs. Absolute values of IOL decentration at 24 hours and 2 weeks after surgery were significantly larger ( P  = 0.008 and 0.046, respectively) in eyes with the single-piece IOL than in ...

This study was conducted to investigate the size and shape of the foveal avascular zone (FAZ) determined by optical coherence tomography angiography (OCTA) and the relationship of the size and shape to the clinical findings in normal subjects. This was a cross-sectional study with seventy eyes of 70 volunteers. The size of the superficial FAZs were assessed by its area, length of perimeter, and Feret’s diameter, and the shape by the circularity, axial ratio, roundness, and solidity. The correlations between each parameter and the clinical findings were statistically determined. The coefficients of variation (CV) of the parameters of FAZ ...

Lacrimal canaliculus (LC) has a key role in tear drainage, but it is difficult to evaluate the LC in detail, using the existing examinations. In this study, our novel LC imaging technique provided the high-resolution images of LC in a non-invasive manner. Three-dimensional images of LC were acquired via the palpebral conjunctiva from 20 healthy volunteers (20 eyes) and 10 patients with various lacrimal disorders (10 eyes), using optical coherence tomography (OCT) dacryography (OCTD). The LC images showed morphological differences between the vertical and horizontal segments. The function of LC could be evaluated by measuring the intralumen signal intensity over ...

Branch retinal vein occlusion (BRVO) may be complicated with retinal ischemia in some cases. The purpose of the current study is to investigate the relationship between optical intensity on optical coherence tomography (OCT) and retinal ischemia in BRVO. Twenty-seven eyes diagnosed with BRVO without macular edema were classified into two groups based on the presence or absence of retinal ischemia. The optical intensity of inner retinal layers and photoreceptor inner segment ellipsoid zone/retinal pigment epithelium layer (ISe/RPE) in the affected and unaffected regions were measured on OCT. Their ratio (Optical intensity ratio, OIR) was calculated and compared between ...

In a cataract surgery, the opacified crystalline lens is replaced by an artificial intraocular lens (IOL). To optimize the visual quality after surgery, the intraocular lens to be implanted must be selected preoperatively for every individual patient. Different generations of formulas have been proposed for selecting the intraocular lens dioptric power as a function of its estimated postoperative position. However, very few formulas include crystalline lens information, in most cases only one-dimensional. The present study proposes a new formula to preoperatively estimate the postoperative IOL position (ELP) based on information of the 3-dimensional full shape of the crystalline lens, obtained ...

Optical coherence tomography angiography (OCTA) allows the retinal microvasculature to be visualized at various retinal depths. Previous studies introduced OCTA axial profile analysis and showed regional variations in the number and location of axially distinct vascular retinal plexuses. OCTA acquisition and processing approaches, however, vary in terms of their resulting transverse and axial resolutions, and especially the latter could potentially influence the profile analysis results. Our study imaged normal eyes using the Spectralis OCT2 with a full-spectrum, probabilistic OCTA algorithm, that, in marked contrast to split-spectrum approaches, preserves the original high OCT axial resolution also within the resulting OCTA signal ...

Purpose We aimed to measure the sizes of Schlemm’s canal (SC) and the trabecular meshwork (TM) in healthy individuals and to evaluate variations with age from childhood to old age by using swept-source optical coherence tomography (OCT). Methods Anterior chamber angle imaging of the superior, inferior, nasal, and temporal regions of the right and left eyes was performed with swept-source OCT. The diameter and area of SC and TM width and thickness were measured manually from OCT images. Results A total of 114 healthy individuals were enrolled and included 48 male subjects and 66 female subjects; their ages ranged ...

Diabetic hyperreflective foci in the outer retinal layers are a clinically relevant finding on optical coherence tomography (OCT) images, although their characteristics remain to be elucidated. Here we investigated the decorrelation signal around hyperreflective foci on OCT angiography (OCTA) images in diabetic retinopathy (DR). We retrospectively reviewed sufficient quality OCTA images from 102 eyes of 66 patients that were obtained using split-spectrum amplitude-decorrelation angiography algorithm. Most confluent hyperreflective foci were randomly deposited or appeared in a radiating array on the en-face structural OCT images in the inner nuclear layer (INL) or Henle’s fiber layer (HFL), respectively. Within the INL ...