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    1. Microstructural characterization of myocardial infarction with optical coherence tractography and two‐photon microscopy

      Microstructural characterization of myocardial infarction with optical coherence tractography and two‐photon microscopy

      Myocardial infarction leads to complex changes in the fiber architecture of the heart. Here, we present a novel optical approach to characterize these changes in intact hearts in three dimensions. Optical coherence tomography (OCT) was used to derive a depth‐resolved field of orientation on which tractography was performed. Tractography of healthy myocardium revealed a smooth linear transition in fiber inclination or helix angle from the epicardium to endocardium. Conversely, in infarcted hearts, no coherent microstructure could be identified in the infarct with OCT. Additional characterization of the infarct was performed by the measurement of light attenuation and with two ...

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    2. Assessment of total retinal blood flow using Doppler Fourier Domain Optical Coherence Tomography during systemic hypercapnia and hypocapnia

      Assessment of total retinal blood flow using Doppler Fourier Domain Optical Coherence Tomography during systemic hypercapnia and hypocapnia

      The purpose of this study was to investigate changes in total retinal blood flow (RBF) using Doppler Fourier Domain Optical Coherence Tomography (Doppler FD‐OCT) in response to the manipulation of systemic partial pressure of CO 2 (P ET CO 2 ). Double circular Doppler blood flow scans were captured in nine healthy individuals (mean age ± standard deviation: 27.1 ± 4.1, six males) using the RTVue ™ FD‐OCT (Optovue). P ET CO 2 was manipulated using a custom‐designed computer‐controlled gas blender (RespirAct ™ ) connected to a sequential gas delivery rebreathing circuit. Doppler FD‐OCT measurements were captured at baseline ...

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