1. Articles in category: Microscopy

    1-24 of 498 1 2 3 4 ... 19 20 21 »
    1. Multi-directional Morphological Assessment of Single Bacterial Colonies Through Non-invasive Optical Imaging

      Multi-directional Morphological Assessment of Single Bacterial Colonies Through Non-invasive Optical Imaging

      A bacterium in bacterial colony is a basal component of bacterial studies and is therefore of considerable importance. The morphological characteristics of a single colony have been widely used as the standard to identify diverse bacterial species. However, the conventional methods for obtaining morphological information, such as microscopic techniques, cannot provide tomographic views. This study utilizes spectral-domain optical coherence tomography (SD-OCT) to observe both external and internal structures for single colonies. OCT imaging provided a clear top (projection) and lateral (cross-sectional) images, which can identify distinct features in the fifteen different bacterial species examined. Through the acquired OCT images, the ...

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    2. Visualization of Bacterial Colonization and Cellular Layers in a Gut-on-a-Chip System Using Optical Coherence Tomography

      Visualization of Bacterial Colonization and Cellular Layers in a Gut-on-a-Chip System Using Optical Coherence Tomography

      Imaging of cellular layers in a gut-on-a-chip system has been confined to two-dimensional (2D)-imaging through conventional light microscopy and confocal laser scanning microscopy (CLSM) yielding three-dimensional- and 2D-cross-sectional reconstructions. However, CLSM requires staining and is unsuitable for longitudinal visualization. Here, we compare merits of optical coherence tomography (OCT) with those of CLSM and light microscopy for visualization of intestinal epithelial layers during protection by a probiotic Bifidobacterium breve strain and a simultaneous pathogen challenge by an Escherichia coli strain. OCT cross-sectional images yielded film thicknesses that coincided with end-point thicknesses derived from cross-sectional CLSM images. Light microscopy on histological ...

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    3. Optical coherence tomography microscopy in experimental traumatic brain injury

      Optical coherence tomography microscopy in experimental traumatic brain injury

      Worldwide elderly traumatic brain injury (TBI) patients tend to become an increasing burden to the society. Thus, a faster and less expensive way of evaluating TBI victims is needed. In the present study we investigated if optical coherence tomography (OCT) could be used as such a method. By using an animal model, we established if OCT can detect cortical changes in the acute phase of a penetrating TBI, in young (5-7 months) and old (20-22 months) rats. Due to the long-term evolution of TBI's, we wanted to investigate to what extent OCT could detect changes within the cortex in ...

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    4. Morphological, optical, and elemental analysis of dental enamel after debonding laminate veneer with Er,Cr:YSGG laser: A pilot study

      Morphological, optical, and elemental analysis of dental enamel after debonding laminate veneer with Er,Cr:YSGG laser: A pilot study

      Laminate veneer removal is becoming a routine procedure at the dental clinic and the use of laser can facilitate its removal. This work aimed to evaluate the morphological, elemental, and optical changes in the remaining enamel after veneer removal using Er,Cr:YSGG laser. Forty‐four enamel slabs were prepared and randomly distributed into nine experimental groups, for bonding using lithium disilicate laminates with three different luting agents (Variolink Veneer, RelyX U200, and RelyX Veneer). Then each agent was debonded using Er,Cr:YSGG laser (2.78 μm) using two different protocols:3.5 W, 48.14 J/cm 2 ...

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    5. High Resolution Multimodal Photoacoustic Microscopy and Optical Coherence Tomography Visualization of Choroidal Vascular Occlusion

      High Resolution Multimodal Photoacoustic Microscopy and Optical Coherence Tomography Visualization of Choroidal Vascular Occlusion

      Photoacoustic microscopy is a novel, non-ionizing, non-invasive imaging technology that evaluates tissue absorption of short-pulsed light through the sound waves emitted by the tissue and has numerous biomedical applications. In this study, a custom-built multimodal imaging system, including photoacoustic microscopy (PAM) and optical coherence tomography (OCT), has been developed to evaluate choroidal vascular occlusion (CVO). CVO was performed on three living rabbits using laser photocoagulation. Longitudinal imaging of CVO was obtained using multiple imaging tools such as color fundus photography, fluorescein angiography, indocyanine green angiography (ICGA), OCT, and PAM. PAM images were acquired at different wavelengths, ranging from 532 to ...

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    6. Role of Microscope-Intraoperative Optical Coherence Tomography in paediatric keratoplasty: A comparative Study

      Role of Microscope-Intraoperative Optical Coherence Tomography in paediatric keratoplasty: A comparative Study

      Purpose To evaluate role of Microscope-Integrated Intraoperative Optical Coherence Tomography (i-OCT) in paediatric keratoplasty. Design Combined prospective and retrospective, comparative, interventional study Setting Dr Rajendra Prasad Centre for Ophthalmic Sciences, India Methods Seventy-five children aged ≤16 years planned for keratoplasty were divided into two groups and compared for intraoperative course and postoperative outcome. In Group-1 (prospective group, n=56), pre-operative anterior segment visualization was performed clinically, with ultrasound biomicroscopy (UBM) and i-OCT and keratoplasty was commenced under i-OCT microscope. In Group-2 (retrospective group, n=19), conventional microscope was employed during keratoplasty. Results In Group-1, i-OCT, UBM and clinical examination detected ...

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    7. Laser Lens Size Measurement Using Swept-Source Optical Coherence Tomography

      Laser Lens Size Measurement Using Swept-Source Optical Coherence Tomography

      A high-speed, high-resolution swept-source optical coherence tomography (SS-OCT) is presented for focusing lens imaging and a k-domain uniform algorithm is adopted to find the wave number phase equalization. The radius of curvature of the laser focusing lens was obtained using a curve-fitting algorithm. The experimental results demonstrate that the measuring accuracy of the proposed SS-OCT system is higher than the laser confocal microscope.

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    8. What Is the Impact of Intraoperative Microscope-Integrated OCT in Ophthalmic Surgery? Relevant Applications and Outcomes. A Systematic Review

      What Is the Impact of Intraoperative Microscope-Integrated OCT in Ophthalmic Surgery? Relevant Applications and Outcomes. A Systematic Review

      Background: Optical coherence tomography (OCT) has recently been introduced in the operating theatre. The aim of this review is to present the actual role of microscope-integrated optical coherence tomography (MI-OCT) in ophthalmology. Method: A total of 314 studies were identified, following a literature search adhering to the preferred reporting items for systematic reviews and meta-analyses (PRISMA) guidelines. After full-text evaluation, 81 studies discussing MI-OCT applications in ophthalmology were included. Results: At present, three microscope-integrated optical coherence tomography systems are commercially available. MI-OCT can help anterior and posterior segment surgeons in the decision-making process, providing direct visualization of anatomic planes before ...

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    9. Light detection and ranging system with photonic integrated circuit

      Light detection and ranging system with photonic integrated circuit

      Disclosed herein are optical integration technologies, designs, systems and methods directed toward Optical Coherence Tomography (OCT) and other interferometric optical sensor, ranging, and imaging systems wherein such systems, methods and structures employ tunable optical sources, coherent detection and other structures on a single or multichip monolithic integration. In contrast to contemporary, prior-art OCT systems and structures that employ simple, miniature optical bench technology using small optical components positioned on a substrate, systems and methods according to the present disclosure employ one or more photonic integrated circuits (PICs), use swept-source techniques, and employ a widely tunable optical source(s). In another ...

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      Mentions: Eric A. Swanson
    10. Vectorial birefringence imaging by optical coherence microscopy for assessing fibrillar microstructures in the cornea and limbus

      Vectorial birefringence imaging by optical coherence microscopy for assessing fibrillar microstructures in the cornea and limbus

      The organization of fibrillar tissue on the micrometer scale carries direct implications for health and disease but remains difficult to assess in vivo . Polarization-sensitive optical coherence tomography measures birefringence, which relates to the microscopic arrangement of fibrillar tissue components. Here, we demonstrate a critical improvement in leveraging this contrast mechanism by employing the improved spatial resolution of focus-extended optical coherence microscopy (1.4 µm axially in air and 1.6 µm laterally, over more than 70 µm depth of field). Vectorial birefringence imaging of sheep cornea ex vivo reveals its lamellar organization into thin sections with distinct local optic axis ...

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    11. Computed optical coherence microscopy of mouse brain ex vivo

      Computed optical coherence microscopy of mouse brain ex vivo

      The compromise between lateral resolution and usable imaging depth range is a bottleneck for optical coherence tomography (OCT). Existing solutions for optical coherence microscopy (OCM) suffer from either large data size and long acquisition time or a nonideal point spread function. We present volumetric OCM of mouse brain ex vivo with a large depth coverage by leveraging computational adaptive optics (CAO) to significantly reduce the number of OCM volumes that need to be acquired with a Gaussian beam focused at different depths. We demonstrate volumetric reconstruction of ex-vivo mouse brain with lateral resolution of 2.2  μm, axial resolution of ...

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    12. Microscope having an optical coherence tomography device

      Microscope having an optical coherence tomography device

      A microscope having an observation beam path including a main objective, an OCT device including a first detection beam path, a wavefront measuring device including a second detection beam path, a first, a second and a third optics group is provided, wherein the first detection beam path contains the main objective and the first to third optics group, and the first to third optics group forms an afocal imaging optical unit of the first detection beam path and the second detection beam path contains the main objective, the first optics group and the second optics group, and the main objective ...

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    13. Combination of 2D Compressive Sensing Spectral Domain Optical Coherence Tomography and Interferometric Synthetic Aperture Microscopy

      Combination of 2D Compressive Sensing Spectral Domain Optical Coherence Tomography and Interferometric Synthetic Aperture Microscopy

      Combining the advantages of compressive sensing spectral domain optical coherence tomography (CS-SDOCT) and interferometric synthetic aperture microscopy (ISAM) in terms of data volume, imaging speed, and lateral resolution, we demonstrated how compressive sampling and ISAM can be simultaneously used to reconstruct an optical coherence tomography (OCT) image. Specifically, an OCT image is reconstructed from two-dimensional (2D) under-sampled spectral data dimension-by-dimension through a CS reconstruction algorithm. During the iterative process of CS algorithm, the deterioration of lateral resolution beyond the depth of focus (DOF) of a Gaussian beam is corrected. In the end, with less spectral data, we can obtain an ...

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    14. VIS-OCT offers sight line

      VIS-OCT offers sight line

      The benefits of optical coherence tomography (OCT) have long been known as an imaging tool in ophthalmology, enabling the examination of the eye at microscopic resolution. But what may not be widely appreciated is that the use of visible light sources can improve upon the results of this well-developed technique in the detection and understanding of various eye-related diseases such as macular degeneration and glaucoma. While most commercial OCT and OCTA (angiography) systems use NIR light for economic and technical reasons, broadband visible light sources have expanded from 400 to 2000 nm or more. This has enabled the imaging of ...

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    15. IMAGING TECHNIQUE FROM BECKMAN LAB NAMED TOP 10 MICROSCOPY INNOVATION

      IMAGING TECHNIQUE FROM BECKMAN LAB NAMED TOP 10 MICROSCOPY INNOVATION

      n imaging technique developed at the Biophotonics Imaging Laboratory at the Beckman Institute was named one of the 10 best microscopy innovations of 2019 by Microscopy Today, the official magazine of the Microscopy Society of America. Dr. Stephen Boppart, a professor of bioengineering and of electrical and computer engineering Simultaneous label-free autofluorescence multi-harmonic (SLAM) microscopy is a new imaging technology developed by senior research scientist Haohua Tu, bioengineering graduate student Sixian You, and a team of researchers at the Biophotonics Imaging Laboratory, led by Dr. Stephen Boppart, a professor of bioengineering and of electrical and computer engineering at Illinois, and ...

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    16. Ophthalmic operation microscope

      Ophthalmic operation microscope

      In an ophthalmic operation microscope, an illumination optical system illuminates a patient's eye with illumination light. An observation optical system is used for observing the patient's eye illuminated. An objective lens is disposed in an observation optical path. An interference optical system splits light from a light source into measurement light and reference light, and detects interference light generated from returning light of the measurement light from the patient's eye and the reference light. A first lens group is disposed between the light source and the patient's eye in an optical path of the measurement light ...

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    17. In vivo corneal confocal microscopy aided by optical coherence tomography

      In vivo corneal confocal microscopy aided by optical coherence tomography

      In vivo corneal confocal microscopy and its operability in scientific as well as in clinical applications is often impaired by the lack of information on imaging plane position and orientation inside the cornea during patient’s examination. To overcome this hurdle, we have developed a novel corneal imaging system based on a commercial scanning device and a modified Rostock Cornea Module. The presented preliminary system produces en face images by confocal laser scanning microscopy and sagittal cross-section images by optical coherence tomography simultaneously. This enables imaging guidance during examinations, improved features for diagnostics along with thickness measurements of the cornea ...

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    18. En face optical coherence tomography: a technology review [Invited]

      En face optical coherence tomography: a technology review [Invited]

      A review on the technological development of en face optical coherence tomography (OCT) and optical coherence microscopy (OCM) is provided. The terminology originally referred to time domain OCT, where the preferential scanning was performed in the en face plane. Potentially the fastest realization of en face image recording is full-field OCT, where the full en face plane is illuminated and recorded simultaneously. The term has nowadays been adopted for high-speed Fourier domain approaches, where the en face image is reconstructed from full 3D volumes either by direct slicing or through axial projection in post processing. The success of modern en ...

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    19. Stereoscopic display systems and methods for displaying surgical data and information in a surgical microscope

      Stereoscopic display systems and methods for displaying surgical data and information in a surgical microscope

      Stereoscopic display systems and methods for displaying surgical data and information in a surgical microscope are disclosed herein. According to an aspect, a system includes first and second eyepieces. The system includes a display having first and second display portions, configured to display first images in the first display portion, and configured to display second images in the second display portion. The first image and the second image are projected along a first pathway and a second pathway. The system includes a first optical element positioned to relay the first images into the first eyepiece. The system includes a second ...

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    20. Optical coherence tomography microscopy apparatus and method for detecting a three-dimensional image of an object

      Optical coherence tomography microscopy apparatus and method for detecting a three-dimensional image of an object

      An optical coherence tomography microscopy apparatus (1) is presented for detecting a three-dimensional image of an optically translucent or reflective sample object (OS), the apparatus comprising an interferometric optical setup including a photo sensor unit (20). A sense signal Si from the photo sensor unit (20) is detected using a detection reference signal. The detection reference signal is derived from a signal indicative for a relative displacement of the sample object (OS) with respect to a reference object.

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    21. Real time dual mode full-field optical coherence microscopy with full range imaging

      Real time dual mode full-field optical coherence microscopy with full range imaging

      The invention is a system and method that enable obtaining ultra-high resolution interference, phase and OCT images at high speed. The system uses neither mechanical moving elements nor any optical/electro optical modulating means for obtaining the OCT images. Two OCT operating modes are available: for ultra-high resolution the system allows either spatial coherence TD-FF-OCT or temporal coherence TD-FF-OCT imaging, whereas for high resolution and ultra-high speed the system allows FD-FF-OCT imaging with full range imaging. In the TD mode, the OCT enface images are obtained in real time. In the FD mode, the 2D complex signal is reconstructed in ...

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    22. Method for correcting an OCT image and combination microscope

      Method for correcting an OCT image and combination microscope

      A method for improving an OCT image of an object such as the retina of an eye, using optical coherence tomography by an imaging beam path. In order to suppress shadowing effects due to a surgical instrument moved in the imaging beam path, a time series of OCT images is produced. For an OCT image to be corrected, an area of the object lying in the image and shadowed by the instrument is determined. Another earlier OCT image in which the area of the object is not shadowed is searched in the time series. Image information for the area of ...

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    23. In Vivo Rat Brain Imaging through Full-Field Optical Coherence Microscopy Using an Ultrathin Short Multimode Fiber Probe

      In Vivo Rat Brain Imaging through Full-Field Optical Coherence Microscopy Using an Ultrathin Short Multimode Fiber Probe

      We demonstrate full-field optical coherence microscopy (OCM) using an ultrathin forward-imaging short multimode fiber (SMMF) probe with a core diameter of 50 μm, outer diameter of 125 μm, and length of 7.4 mm, which is a typical graded-index multimode fiber used for optical communications. The axial and lateral resolutions were measured to be 2.14 μm and 2.3 μm, respectively. By inserting the SMMF 4 mm into the cortex of an in vivo rat brain, scanning was performed to a depth of 147 μm from the SMMF facet with a field of view of 47 μm. Three-dimensional (3D ...

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    24. In vivo confocal microscopy and anterior segment optical coherence tomography follow-up of cysteamine treatment in corneal cystinosis

      In vivo confocal microscopy and anterior segment optical coherence tomography follow-up of cysteamine treatment in corneal cystinosis

      A 36-year-old female presented initially with photophobia and visual deterioration. After examination and laboratory tests, patient was diagnosed with cystinosis. Cysteamine drops 4 × 1 drops/day was given as treatment for 1 year. During follow-up, in vivo confocal microscopy (IVCM) and anterior segment optical coherence tomography (AS-OCT) was performed. Photophobia was relieved and IVCM obtained the decrease in size and density of corneal crystals 1 year after. Depth of corneal crystals did not change but crystal density score reduced with cysteamine treatment.

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    1-24 of 498 1 2 3 4 ... 19 20 21 »
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