1. Articles in category: Microscopy

    1-24 of 482 1 2 3 4 ... 18 19 20 »
    1. En face optical coherence tomography: a technology review [Invited]

      En face optical coherence tomography: a technology review [Invited]

      A review on the technological development of en face optical coherence tomography (OCT) and optical coherence microscopy (OCM) is provided. The terminology originally referred to time domain OCT, where the preferential scanning was performed in the en face plane. Potentially the fastest realization of en face image recording is full-field OCT, where the full en face plane is illuminated and recorded simultaneously. The term has nowadays been adopted for high-speed Fourier domain approaches, where the en face image is reconstructed from full 3D volumes either by direct slicing or through axial projection in post processing. The success of modern en ...

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    2. Stereoscopic display systems and methods for displaying surgical data and information in a surgical microscope

      Stereoscopic display systems and methods for displaying surgical data and information in a surgical microscope

      Stereoscopic display systems and methods for displaying surgical data and information in a surgical microscope are disclosed herein. According to an aspect, a system includes first and second eyepieces. The system includes a display having first and second display portions, configured to display first images in the first display portion, and configured to display second images in the second display portion. The first image and the second image are projected along a first pathway and a second pathway. The system includes a first optical element positioned to relay the first images into the first eyepiece. The system includes a second ...

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    3. Optical coherence tomography microscopy apparatus and method for detecting a three-dimensional image of an object

      Optical coherence tomography microscopy apparatus and method for detecting a three-dimensional image of an object

      An optical coherence tomography microscopy apparatus (1) is presented for detecting a three-dimensional image of an optically translucent or reflective sample object (OS), the apparatus comprising an interferometric optical setup including a photo sensor unit (20). A sense signal Si from the photo sensor unit (20) is detected using a detection reference signal. The detection reference signal is derived from a signal indicative for a relative displacement of the sample object (OS) with respect to a reference object.

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    4. Real time dual mode full-field optical coherence microscopy with full range imaging

      Real time dual mode full-field optical coherence microscopy with full range imaging

      The invention is a system and method that enable obtaining ultra-high resolution interference, phase and OCT images at high speed. The system uses neither mechanical moving elements nor any optical/electro optical modulating means for obtaining the OCT images. Two OCT operating modes are available: for ultra-high resolution the system allows either spatial coherence TD-FF-OCT or temporal coherence TD-FF-OCT imaging, whereas for high resolution and ultra-high speed the system allows FD-FF-OCT imaging with full range imaging. In the TD mode, the OCT enface images are obtained in real time. In the FD mode, the 2D complex signal is reconstructed in ...

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    5. Method for correcting an OCT image and combination microscope

      Method for correcting an OCT image and combination microscope

      A method for improving an OCT image of an object such as the retina of an eye, using optical coherence tomography by an imaging beam path. In order to suppress shadowing effects due to a surgical instrument moved in the imaging beam path, a time series of OCT images is produced. For an OCT image to be corrected, an area of the object lying in the image and shadowed by the instrument is determined. Another earlier OCT image in which the area of the object is not shadowed is searched in the time series. Image information for the area of ...

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    6. In Vivo Rat Brain Imaging through Full-Field Optical Coherence Microscopy Using an Ultrathin Short Multimode Fiber Probe

      In Vivo Rat Brain Imaging through Full-Field Optical Coherence Microscopy Using an Ultrathin Short Multimode Fiber Probe

      We demonstrate full-field optical coherence microscopy (OCM) using an ultrathin forward-imaging short multimode fiber (SMMF) probe with a core diameter of 50 μm, outer diameter of 125 μm, and length of 7.4 mm, which is a typical graded-index multimode fiber used for optical communications. The axial and lateral resolutions were measured to be 2.14 μm and 2.3 μm, respectively. By inserting the SMMF 4 mm into the cortex of an in vivo rat brain, scanning was performed to a depth of 147 μm from the SMMF facet with a field of view of 47 μm. Three-dimensional (3D ...

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    7. In vivo confocal microscopy and anterior segment optical coherence tomography follow-up of cysteamine treatment in corneal cystinosis

      In vivo confocal microscopy and anterior segment optical coherence tomography follow-up of cysteamine treatment in corneal cystinosis

      A 36-year-old female presented initially with photophobia and visual deterioration. After examination and laboratory tests, patient was diagnosed with cystinosis. Cysteamine drops 4 × 1 drops/day was given as treatment for 1 year. During follow-up, in vivo confocal microscopy (IVCM) and anterior segment optical coherence tomography (AS-OCT) was performed. Photophobia was relieved and IVCM obtained the decrease in size and density of corneal crystals 1 year after. Depth of corneal crystals did not change but crystal density score reduced with cysteamine treatment.

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    8. In Vivo and Ex Vivo Microscopy: Moving Toward the Integration of Optical Imaging Technologies Into Pathology Practice

      In Vivo and Ex Vivo Microscopy: Moving Toward the Integration of Optical Imaging Technologies Into Pathology Practice

      The traditional surgical pathology assessment requires tissue to be removed from the patient, then processed, sectioned, stained, and interpreted by a pathologist using a light microscope. Today, an array of alternate optical imaging technologies allow tissue to be viewed at high resolution, in real time, without the need for processing, fixation, freezing, or staining. Optical imaging can be done in living patients without tissue removal, termed in vivo microscopy, or also in freshly excised tissue, termed ex vivo microscopy. Both in vivo and ex vivo microscopy have tremendous potential for clinical impact in a wide variety of applications. However, in ...

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    9. 250 kHz, 1.5 µm resolution SD-OCT for in-vivo cellular imaging of the human cornea

      250 kHz, 1.5 µm resolution SD-OCT for in-vivo cellular imaging of the human cornea

      We present the first spectral domain optical coherence tomography (SD-OCT) system that combines an isotropic imaging resolution of ~1.5 µm in biological tissue with a 250 kHz image acquisition rate, for in vivo non-contact, volumetric imaging of the cellular structure of the human cornea. OCT images of the healthy human cornea acquired with this system reveal the cellular structure of the corneal epithelium, cellular debris and mucin clusters in the tear film, the shape, size and spatial distribution of the sub-basal corneal nerves and keratocytes in the corneal stroma, as well as reflections from endothelial nuclei. The corneal images ...

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    10. Superparamagnetic graphene quantum dot as a dual‐modality contrast agent for confocal fluorescence microscopy and magnetomotive optical coherence tomography

      Superparamagnetic graphene quantum dot as a dual‐modality contrast agent for confocal fluorescence microscopy and magnetomotive optical coherence tomography

      A magnetic graphene quantum dot (MGQD) nanoparticle, synthesized by hydrothermally reducing and cutting graphene oxide‐iron oxide sheet, was demonstrated to possess the capabilities of simultaneous confocal fluorescence and magnetomotive optical coherence tomography (MMOCT) imaging. This MGQD shows low toxicity, significant tunable blue fluorescence and superparamagnetism, which can thus be used as a dual‐modality contrast agent for confocal fluorescence microscopy (CFM) and MMOCT. The feasibility of applying MGQD as a tracer of cells is shown by imaging and visualizing MGQD labeled cells using CFM and our in‐house MMOCT. Since MMOCT and CFM can offer anatomical structure and intracellular ...

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    11. Computer-Aided Diagnosis of Label-Free 3-D Optical Coherence Microscopy Images of Human Cervical Tissue

      Computer-Aided Diagnosis of Label-Free 3-D Optical Coherence Microscopy Images of Human Cervical Tissue

      : Ultrahigh-resolution optical coherence microscopy (OCM) has recently demonstrated its potential for accurate diagnosis of human cervical diseases. One major challenge for clinical adoption, however, is the steep learning curve clinicians need to overcome to interpret OCM images. Developing an intelligent technique for computer-aided diagnosis (CADx) to accurately interpret OCM images will facilitate clinical adoption of the technology and improve patient care. Methods: 497 high-resolution 3-D OCM volumes (600 cross-sectional images each) were collected from 159 ex vivo specimens of 92 female patients. OCM image features were extracted using a convolutional neural network (CNN) model, concatenated with patient information (e.g ...

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    12. Multimodal Optical Imaging for Pretreatment Evaluation for Cutaneous Microparticle Delivery

      Multimodal Optical Imaging for Pretreatment Evaluation for Cutaneous Microparticle Delivery

      Brief Summary: The purpose of this study is to investigate how pretreatment with a topical medication for acne affects application and delivery of gold microparticles to the skin in acne patients. In addition, the investigators want to investigate the biodistribution of gold microparticles in pretreated skin and compare this with the biodistribution of untreated skin using the non-invasive imaging techniques optical coherence tomography and reflectance confocal microscopy. Reflectance Confocal Microscopy (RCM) and Optical Coherence Tomography (OCT) are non-invasive imaging technologies allowing for imaging of the upper skin layers. Gold microparticles have been shown to have a contrast effect on the ...

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    13. Volumetric optical coherence microscopy with a high space-bandwidth-time product enabled by hybrid adaptive optics

      Volumetric optical coherence microscopy with a high space-bandwidth-time product enabled by hybrid adaptive optics

      Optical coherence microscopy (OCM) is a promising modality for high resolution imaging, but has limited ability to capture large-scale volumetric information about dynamic biological processes with cellular resolution. To enhance the throughput of OCM, we implemented a hybrid adaptive optics (hyAO) approach that combines computational adaptive optics with an intentionally aberrated imaging beam generated via hardware adaptive optics. Using hyAO, we demonstrate the depth-equalized illumination and collection ability of an astigmatic beam compared to a Gaussian beam for cellular-resolution imaging. With this advantage, we achieved volumetric OCM with a higher space-bandwidth- time product compared to Gaussian-beam acquisition that employed focus-scanning ...

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    14. Surface emitting laser, laser array, light source device, information acquisition device, and optical coherence tomography apparatus

      Surface emitting laser, laser array, light source device, information acquisition device, and optical coherence tomography apparatus

      Provided is a surface emitting laser that is to be excited by an external light source and in which a light-emitting position is defined. A surface emitting laser includes a pair of reflecting mirrors (11, 15) and an active layer (13) that is arranged between the pair of reflecting mirrors (11, 15) and that is to be excited by light that is radiated from an external light source. A gap is formed between the active layer and one of the pair of reflecting mirrors (15), the oscillation wavelength of the surface emitting laser is changed, and a defining structure (20 ...

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    15. Microscopy system with auto-focus adjustment by low-coherence interferometry

      Microscopy system with auto-focus adjustment by low-coherence interferometry

      Disclosed are several technical approaches of using low coherence interferometry techniques to create an autofocus apparatus for optical microscopy. These approaches allow automatic focusing on thin structures that are positioned closely to reflective surfaces and behind refractive material like a cover slip, and automated adjustment of focus position into the sample region without disturbance from reflection off adjacent surfaces. The measurement offset induced by refraction of material that covers the sample is compensated for. Proposed are techniques of an instrument that allows the automatic interchange of imaging objectives in a low coherence interferometry autofocus system, which is of major interest ...

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    16. Optical coherence tomography systems integrated with surgical microscopes

      Optical coherence tomography systems integrated with surgical microscopes

      Some embodiments of the present inventive concept provide optical coherence tomography (OCT) systems for integration with a microscope. The OCT system includes a sample arm coupled to the imaging path of a microscope. The sample arm includes an input beam zoom assembly including at least two movable lenses configured to provide shape control for an OCT signal beam; a scan assembly including at least one scanning mirror and configured for telecentric scanning of the OCT signal beam; and a beam expander configured to set the OCT signal beam diameter incident on the microscope objective. The shape control includes separable controls ...

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    17. Clinical Translation of Tethered Confocal Microscopy Capsule for Unsedated Diagnosis of Eosinophilic Esophagitis

      Clinical Translation of Tethered Confocal Microscopy Capsule for Unsedated Diagnosis of Eosinophilic Esophagitis

      Esophagogastroduodenoscopy (EGD) is a widely used procedure, posing significant financial burden on both healthcare systems and patients. Moreover, EGD is time consuming, sometimes difficult to tolerate, and suffers from an imperfect diagnostic yield as the limited number of collected biopsies does not represent the whole organ. In this paper, we report on technological and clinical feasibility of a swallowable tethered endomicroscopy capsule, which is administered without sedation, to image large regions of esophageal and gastric mucosa at the cellular level. To demonstrate imaging capabilities, we conducted a human pilot study (n = 17) on Eosinophilic Esophagitis (EoE) patients and healthy volunteers ...

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    18. Actinic Keratosis and Non-Invasive Diagnostic Techniques: An Update

      Actinic Keratosis and Non-Invasive Diagnostic Techniques: An Update

      Actinic keratosis represents the earliest manifestation of non-melanoma skin cancer. Because of their risk of progression to invasive squamous cell carcinoma, an earlier diagnosis and treatment are mandatory. Their diagnosis sometimes could represent a challenge even for expert dermatologists. Dermoscopy, confocal laser microscopy and optical coherence tomography could help clinicians in diagnosis. .

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    19. Visible Light Optical Coherence Microscopy of the Brain with Isotropic Femtoliter Resolution In Vivo

      Visible Light Optical Coherence Microscopy of the Brain with Isotropic Femtoliter Resolution In Vivo

      Most flying-spot Optical Coherence Tomography (OCT) and Optical Coherence Microscopy (OCM) systems use a symmetric confocal geometry, where the detection path retraces the illumination path starting from and ending with the spatial mode of a single mode optical fiber. Here, we describe a visible light OCM instrument that breaks this symmetry to improve transverse resolution without sacrificing collection efficiency. This was achieved by overfilling a water immersion objective on the illumination path, while maintaining a conventional Gaussian mode detection path (1/e2 intensity diameter ~0.82 Airy disks), enabling ~1.1 μm full-width at half-maximum (FWHM) transverse resolution. At the ...

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    20. Interferometric synthetic aperture microscopy for extended focus optical coherence microscopy

      Interferometric synthetic aperture microscopy for extended focus optical coherence microscopy

      Optical coherence microscopy (OCM) is an interferometric technique providing 3D images of biological samples with micrometric resolution and penetration depth of several hundreds of micrometers. OCM differs from optical coherence tomography (OCT) in that it uses a high numerical aperture (NA) objective to achieve high lateral resolution. However, the high NA also reduces the depth-of-field (DOF), scaling with 1/NA 2 . Interferometric synthetic aperture microscopy (ISAM) is a computed imaging technique providing a solution to this trade-off between resolution and DOF. An alternative hardware method to achieve an extended DOF is to use a non-Gaussian illumination. Extended focus OCM (xfOCM ...

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    21. Surgical microscope with integrated optical coherence tomography and display systems

      Surgical microscope with integrated optical coherence tomography and display systems

      An ophthalmic surgical microscope includes a beam coupler positioned along an optical path of the surgical microscope between a first eyepiece and magnifying/focusing optics, the beam coupler operable to direct the OCT imaging beam along a first portion of the optical path of the surgical microscope between the beam coupler and a patient's eye (an OCT image being generated based on a reflected portion of the OCT imaging beam). The surgical microscope additionally includes a real-time data projection unit operable to project the OCT image generated by the OCT system and a beam splitter positioned along the optical ...

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      Mentions: Lingfeng Yu
    22. Real-time and non-invasive measurements of cell mechanical behaviour with optical coherence phase microscopy

      Real-time and non-invasive measurements of cell mechanical behaviour with optical coherence phase microscopy

      Cell mechanical behaviour is increasingly recognised as a central biophysical parameter in cancer and stem cell research, and methods of investigating their mechanical behaviour are therefore needed. We have developed a novel qualitative method based on quantitative phase imaging which is capable of investigating cell mechanical behaviour in real-time at cellular resolution using optical coherence phase microscopy (OCPM), and stimulating the cells non-invasively using hydrostatic pressure. The method was exemplified to distinguish between cells with distinct mechanical properties, and transient change induced by Cytochalasin D. We showed the potential of quantitative phase imaging to detect nanoscale intracellular displacement induced by ...

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    23. Spectrally Encoded Coherence Tomography and Reflectometry (SECTR): simultaneous en face and cross-sectional imaging at 2 gigapixels-per-second

      Spectrally Encoded Coherence Tomography and Reflectometry (SECTR): simultaneous en face and cross-sectional imaging at 2 gigapixels-per-second

      Non-invasive biological imaging is crucial for understanding in vivo structure and function. Optical coherence tomography (OCT) and reflectance confocal microscopy are two of the most widely used optical modalities for exogenous contrast-free high-resolution three-dimensional imaging in non-fluorescent scattering tissues. However, sample motion remains a critical barrier to raster-scanned acquisition and reconstruction of wide-field anatomically accurate volumetric datasets. We introduce spectrally encoded coherence tomography and reflectometry (SECTR), a high-speed multimodality system for simultaneous OCT and spectrally-encoded reflectance (SER) imaging. SECTR utilizes a robust system design consisting of shared optical relays, scanning mirrors, swept-laser, and digitizer to achieve the fastest reported in ...

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    1-24 of 482 1 2 3 4 ... 18 19 20 »
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