A surgical imaging system is provided. The surgical imaging system includes a surgical microscope and a telecentric optical coherence tomography scanning unit configured to scan a sample through at least one optical component associated with the surgical microscope.
The transfer function for optical wavefront aberrations in single-mode fiber based optical coherence tomography is determined. The loss in measured OCT signal due to optical wavefront aberrations is quantified using Fresnel propagation and the calculation of overlap integrals. A distinction is made between a model for a mirror and a scattering medium model. The model predictions are validated with measurements on a mirror and a scattering medium obtained with an adaptive optics optical coherence tomography setup. Furthermore, a one-step defocus correction, based on a single A-scan measurement, is derived from the model and verified. Finally, the pseudo-convex structure of the ...
A 62-year-old man presented with a 9-month history of a bleeding lesion. Clinical evaluation showed a 5-mm red-to-black keratotic papule on the first digit of the left foot (Fig 1, A).
Dynamic near infrared microscopy has revealed transient retinal phototropism (TRP) correlated with oblique light stimulation. Here, by developing a hybrid confocal microscopy and optical coherence tomography (OCT), we tested sub-cellular source of the TRP in living frog retina. Dynamic confocal microscopy and OCT consistently revealed photoreceptor outer segments as the anatomic source of the TRP. Further investigation of the TRP can provide insights in better understanding of Stiles–Crawford effect (SCE) on rod and cone systems, and may also promise an intrinsic biomarker for early detection of eye diseases that can produce photoreceptor dysfunction.
Current technique for tumor resection requires biopsy of the tumor region and histological confirmation before the surgeon can be certain that the entire tumor has been resected. This confirmation process is time consuming both for the surgeon and the patient and also requires sacrifice of healthy tissue, motivating the development of novel technologies which can enable real-time detection of tumor-healthy tissue boundary for faster and more efficient surgeries. In this study, the potential of combining structural information from optical coherence tomography (OCT) and molecular information from line-scan Raman microscopy (LSRM) for such an application is presented. The results show a ...
A microscopy system and method allow observing a fluorescent substance accumulated in a tissue. The tissue can be observed at a same time both with visible light and with fluorescent light. It is possible to observe a series of previously recorded fluorescent light images in superposition with the visible light images. An end of the series of images may be automatically determined. A thermal protective filter may be inserted into a beam path of an illuminating system at such automatically determined end of the series. Further, the fluorescent light image may be analyzed for identifying a coherent fluorescent portion thereof ...
Exemplary embodiments of systems and methods can be provided which can generate data associated with at least one portion of a sample. For example, at least one first radiation can be forwarded to the portion through at least one optical arrangement. At least one second radiation can be received from the portion which is based on the first radiation. Based on an interaction between the optical arrangement and the first radiation and/or the second radiation, the optical arrangement can have a first transfer function. Further, it is possible to forward at least one third radiation to the portion through ...
Yale University Receives 2014 NIH Grant for $209,850 for Massively Parallel Interferometric Confocal Microscopy using Degenerate Lasers. The principal nvestigator is Michael Choma. Below is a summary of the proposed work. Confocal microscopy uses geometric principles to generate cross-sectional images of scattering biological tissue. Traditional reflectance confocal microscopes use serial pixel acquisition, thereby limiting acquisition speeds. Parallelization of pixel acquisition would dramatically increase imaging speeds and enable the study of new kinds of physiology. For example, massively parallelized reflectance confocal microscopy would be invaluable in the emerging field of cilia-driven mucus physiology in the respiratory system. Indeed, in terms ...
hree-dimensional high-resolution imaging methods are important for cellular-level research. Optical coherence microscopy (OCM) is a low-coherence-based interferometry technology for cellular imaging with both high axial and lateral resolution. Using a high-numerical-aperture objective, OCM normally has a shallow depth of field and requires scanning the focus through the entire region of interest to perform volumetric imaging. With a higher-numerical-aperture objective, the image quality of OCM is affected by and more sensitive to aberrations. Interferometric synthetic aperture microscopy (ISAM) and computational adaptive optics (CAO) are computed imaging techniques that overcome the depth-of-field limitation and the effect of optical aberrations in optical coherence ...
Optical coherence tomography (OCT) is one of several exciting, advanced imaging techniques that are beginning to appear in pathology. OCT can rapidly generate photomicrograph-like images directly from tissue, without the need for glass slide histology. Because the images resemble low-to-medium magnification microscope images, pathologists should be able to adapt their existing diagnostic expertise to these novel images. Both in-vivo and specimen (ex-vivo) applications are possible; this means that advanced imaging may permit pathologists to make diagnoses directly on patient images. Advanced imaging therefore represents a fabulous opportunity for pathologists in the 21st century. This talk will focus on specimen OCT ...
A dual-modality apparatus for imaging of biological tissue includes a reflectance confocal microscopy (RCM) imaging apparatus and an optical coherence tomography (OCT) imaging apparatus. A first optical component reflects a first beam of light provided by a RCM imaging apparatus towards a sample and passes a second beam of light provided by an OCT imaging apparatus towards the sample, such that the first and second beam of lights share at least a portion of an imaging path.
Various approaches are disclosed for simultaneously generating optical coherence tomography (OCT) and confocal scanning laser images by spatially separating the signal normally used for OCT imaging with selective reflecting or beam directing devices. In one preferred embodiment, the invention includes a mirror having a central transmission region, such that the radially outer region of the returning signal beam is reflected and used for generating a confocal image while the central part of the signal beam is transmitted and used to generate an OCT image. In other embodiments, the signals may be spatially separated in other ways, such as with an ...
Recent decades have seen an expansion in the breadth of light-based imaging techniques that address problems in biomedicine. Despite perceived limitations in terms of penetration depth and resolution, optical imaging has made significant strides to encroach upon the traditional territory of other modalities, such as electron microscopy and x-ray. At the nanoscale, optical methods exploit light fields in tandem with the properties of fluorescent proteins to answer biological questions in neuroscience and virology that were previously restricted to electron microscopy. At the larger scale, the same methods are making headway to generate biopsies solely with light. A prime example is ...
Some embodiments of the present inventive concept provide optical coherence tomography (OCT) systems for integration with a microscope. The OCT system includes a sample arm coupled to the imaging path of a microscope. The sample arm includes an input beam zoom assembly including at least two movable lenses configured to provide shape control for an OCT signal beam; a scan assembly including at least one scanning mirror and configured for telecentric scanning of the OCT signal beam; and a beam expander configured to set the OCT signal beam diameter incident on the microscope objective. The shape control includes separable controls ...
Super-resolution microscopes from Carl Zeiss and Leica Microsystems are among more than a dozen optics and photonics innovations to win one of this year’s R&D 100 Awards. Intended to recognize the 100 “most technologically significant products” introduced over the past year, the list also includes an all-fiber isolator from AdValue Photonics, an optical coherence tomography (OCT) diagnostic endoscope developed by NinePoint Medical, and a lunar laser communication system co-developed by NASA and MIT’s Lincoln Laboratory. In the microscopy sector, the R&D Magazine judging panel selected several winners, with Zeiss noted for the latest incarnation of its ...
Physical Sciences Incorporated Received a 2014 NIH Grant for $538,335 for Optical Coherence Tomography Based on Freeze Drying Microscopy. The principal investigator is Mircea Mujat. The program began in 2009 and ends in 2015. Below is a summary of the proposed work. Physical Sciences Inc. (PSI), in collaboration with the University of Connecticut (UConn), proposes to develop the 2nd generation of an advanced 3D freeze-drying microscopy (FDM) system to advance the understanding of freeze-drying and to enable the development of efficient commercial freeze drying processes. During the Phase I program PSI demonstrated the application of Optical Coherence Tomography (OCT ...
There is an inherent trade-off between transverse resolution and depth of field (DOF) in optical coherence tomography (OCT) which becomes a limiting factor for certain applications. Multifocal OCT and interferometric synthetic aperture microscopy (ISAM) each provide a distinct solution to the trade-off through modification to the experiment or via post-processing, respectively. In this paper, we have solved the inverse problem of multifocal OCT and present a general algorithm for combining multiple ISAM datasets. Multifocal ISAM (MISAM) uses a regularized combination of the resampled datasets to bring advantages of both multifocal OCT and ISAM to achieve optimal transverse resolution, extended effective ...
Exemplary method, apparatus and arrangement can be provided for obtaining information associated with a sample such as a portion of an anatomical structure. The information can be generated using first data, which can be based on a signal obtained from a location on the sample, and second data, where the second data can be obtained by combining a second signal received from the sample with a third reference signal. An image of a portion of the sample can also be generated based on the information. For example, the first data can be associated with spectral encoding microscopy data, and the ...
The advent of Optical Coherence Tomography (OCT) in ophthalmology brought a more accurate diagnosis, earlier detection of disease, and more fine-tuned treatment of individual patients’ diseases. Evidence has demonstrated that intraoperative OCT (iOCT) can provide surgeons with crucial imaging of structures below the visible surface. The DISCOVER study was created to evaluate iOCT in eye surgeries. Preliminary results from the study were recently published in the British Journal of Ophthalmology. These results looked at how a specific iOCT system (RESCAN 700) was used in 10 surgeries. The RESCAN 700 features a heads-up display and foot controls for ease of use ...
The advent of scanning electron microscopy has facilitated our understanding of the biology in relation to surface microstructure of many invertebrates. In recent years, interest in biomimetics and bio-inspired materials has further propelled the search for novel microstructures from natural surfaces. As this search widens in diversity to nurture deeper understanding of form and function, the need often arises to examine rare specimens. Unfortunately, most methods for characterization of the microtopography of natural surfaces are sacrificial, and as such, place limiting constraints on research progress in situations where only a few rare specimens are known, such as the rich resources ...
We present novel optical and mechanical designs for a microscope-integrated intraoperative optical coherence tomography (iOCT) system with enhanced function and ergonomics for visualization of ophthalmic surgical maneuvers. Integration of an electrically tunable lens allows rapid focal plane adjustment and iOCT imaging of both anterior and posterior segment tissue microstructures while maintaining parfocality with the ophthalmic surgical microscope. We demonstrate novel visualization of instrument positions relative to tissue layers of interest as colormap overlays onto en face OCT data, which may provide integrative display of volumetric information during surgical maneuvers. Finally, we implement a heads-up display system to provide real-time feedback ...
Methods for correcting for aberrations in the image or three-dimensional reconstruction of a sampled region obtained by broadband interferometry. The sampled region is illuminated with a broadband beam of light, and light returned from the sample is detected, along with a reference beam, in order to derive an interference signal for pixels of a volume spanned by wavenumber and axes transverse to the beam propagation direction. An optimization procedure is performed with respect to a specified criterion so as to obtain an aberration-corrected image of at least one plane of the sampled region, either in a plane-specific manner or in ...
Quantitative measurement of dynamic responses of unstained living cells is of great importance in many applications ranging from investigation of fundamental cellular functions to drug discoveries. Conventional optical methods for label-free cell-based assay examine cellular structural changes proximal to sensor surfaces under external stimuli, but require dedicated nanostructure-patterned substrates for operation. Here, we present a quantitative imaging method, spectral-domain optical coherence phase microscopy (SD-OCPM), as a viable optical platform for label-free cell-based assay. The instrument is based on a low-coherence interferometric microscope that enables quantitative depth-resolved phase measurement of a transparent specimen with high phase stability. We demonstrate SD-OCPM measurement ...
Significant integrative advances are needed for intraoperative optical coherence tomography ( i OCT) to achieve widespread use across ophthalmic surgery. A surgeon feedback system that provides microscope integration, heads-up display and foot pedal control of the OCT scan location represents a major interval advance in ophthalmic surgery. In this report, we describe the preliminary findings of the Determination of feasibility of Intraoperative Spectral domain microscope Combined/integrated OCT Visualisation during En face Retinal and ophthalmic surgery (DISCOVER) study, a multisurgeon investigational device study examining the role of microscope integrated i OCT systems with surgeon heads-up display feedback (eg, Carl Zeiss Meditec ...
