1. Articles in category: Microscopy

    1-24 of 376 1 2 3 4 ... 14 15 16 »
    1. Two-dimensional confocal imaging using OCT light source and scan optics

      Two-dimensional confocal imaging using OCT light source and scan optics

      Various approaches are disclosed for simultaneously generating optical coherence tomography (OCT) and confocal scanning laser images by spatially separating the signal normally used for OCT imaging with selective reflecting or beam directing devices. In one preferred embodiment, the invention includes a mirror having a central transmission region, such that the radially outer region of the returning signal beam is reflected and used for generating a confocal image while the central part of the signal beam is transmitted and used to generate an OCT image. In other embodiments, the signals may be spatially separated in other ways, such as with an ...

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    2. Light sheet imaging the Airy way

      Light sheet imaging the Airy way

      Recent decades have seen an expansion in the breadth of light-based imaging techniques that address problems in biomedicine. Despite perceived limitations in terms of penetration depth and resolution, optical imaging has made significant strides to encroach upon the traditional territory of other modalities, such as electron microscopy and x-ray. At the nanoscale, optical methods exploit light fields in tandem with the properties of fluorescent proteins to answer biological questions in neuroscience and virology that were previously restricted to electron microscopy. At the larger scale, the same methods are making headway to generate biopsies solely with light. A prime example is ...

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    3. Surgical microscopes using optical coherence tomography and related methods

      Surgical microscopes using optical coherence tomography and related methods

      Some embodiments of the present inventive concept provide optical coherence tomography (OCT) systems for integration with a microscope. The OCT system includes a sample arm coupled to the imaging path of a microscope. The sample arm includes an input beam zoom assembly including at least two movable lenses configured to provide shape control for an OCT signal beam; a scan assembly including at least one scanning mirror and configured for telecentric scanning of the OCT signal beam; and a beam expander configured to set the OCT signal beam diameter incident on the microscope objective. The shape control includes separable controls ...

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    4. Super-resolution and origami microscopes scoop R&D 100 awards

      Super-resolution and origami microscopes scoop R&D 100 awards

      Super-resolution microscopes from Carl Zeiss and Leica Microsystems are among more than a dozen optics and photonics innovations to win one of this year’s R&D 100 Awards. Intended to recognize the 100 “most technologically significant products” introduced over the past year, the list also includes an all-fiber isolator from AdValue Photonics, an optical coherence tomography (OCT) diagnostic endoscope developed by NinePoint Medical, and a lunar laser communication system co-developed by NASA and MIT’s Lincoln Laboratory. In the microscopy sector, the R&D Magazine judging panel selected several winners, with Zeiss noted for the latest incarnation of its ...

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    5. Physical Sciences Receives NIH Grant for Optical Coherence Tomography Based on Freeze Drying Microscopy

      Physical Sciences Receives NIH Grant for Optical Coherence Tomography Based on Freeze Drying Microscopy

      Physical Sciences Incorporated Received a 2014 NIH Grant for $538,335 for Optical Coherence Tomography Based on Freeze Drying Microscopy. The principal investigator is Mircea Mujat. The program began in 2009 and ends in 2015. Below is a summary of the proposed work. Physical Sciences Inc. (PSI), in collaboration with the University of Connecticut (UConn), proposes to develop the 2nd generation of an advanced 3D freeze-drying microscopy (FDM) system to advance the understanding of freeze-drying and to enable the development of efficient commercial freeze drying processes. During the Phase I program PSI demonstrated the application of Optical Coherence Tomography (OCT ...

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    6. Multifocal interferometric synthetic aperture microscopy

      Multifocal interferometric synthetic aperture microscopy

      There is an inherent trade-off between transverse resolution and depth of field (DOF) in optical coherence tomography (OCT) which becomes a limiting factor for certain applications. Multifocal OCT and interferometric synthetic aperture microscopy (ISAM) each provide a distinct solution to the trade-off through modification to the experiment or via post-processing, respectively. In this paper, we have solved the inverse problem of multifocal OCT and present a general algorithm for combining multiple ISAM datasets. Multifocal ISAM (MISAM) uses a regularized combination of the resampled datasets to bring advantages of both multifocal OCT and ISAM to achieve optimal transverse resolution, extended effective ...

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    7. Method and apparatus for optical imaging via spectral encoding

      Method and apparatus for optical imaging via spectral encoding

      Exemplary method, apparatus and arrangement can be provided for obtaining information associated with a sample such as a portion of an anatomical structure. The information can be generated using first data, which can be based on a signal obtained from a location on the sample, and second data, where the second data can be obtained by combining a second signal received from the sample with a third reference signal. An image of a portion of the sample can also be generated based on the information. For example, the first data can be associated with spectral encoding microscopy data, and the ...

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    8. RESCAN 700 allows for real time imaging in eye surgery

      RESCAN 700 allows for real time imaging in eye surgery

      The advent of Optical Coherence Tomography (OCT) in ophthalmology brought a more accurate diagnosis, earlier detection of disease, and more fine-tuned treatment of individual patients’ diseases. Evidence has demonstrated that intraoperative OCT (iOCT) can provide surgeons with crucial imaging of structures below the visible surface. The DISCOVER study was created to evaluate iOCT in eye surgeries. Preliminary results from the study were recently published in the British Journal of Ophthalmology. These results looked at how a specific iOCT system (RESCAN 700) was used in 10 surgeries. The RESCAN 700 features a heads-up display and foot controls for ease of use ...

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    9. Optical coherence tomography as a tool for characterization of complex biological surfaces

      Optical coherence tomography as a tool for characterization of complex biological surfaces

      The advent of scanning electron microscopy has facilitated our understanding of the biology in relation to surface microstructure of many invertebrates. In recent years, interest in biomimetics and bio-inspired materials has further propelled the search for novel microstructures from natural surfaces. As this search widens in diversity to nurture deeper understanding of form and function, the need often arises to examine rare specimens. Unfortunately, most methods for characterization of the microtopography of natural surfaces are sacrificial, and as such, place limiting constraints on research progress in situations where only a few rare specimens are known, such as the rich resources ...

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    10. Microscope-integrated intraoperative OCT with electrically tunable focus and heads-up display for imaging of ophthalmic surgical maneuvers

      Microscope-integrated intraoperative OCT with electrically tunable focus and heads-up display for imaging of ophthalmic surgical maneuvers

      We present novel optical and mechanical designs for a microscope-integrated intraoperative optical coherence tomography (iOCT) system with enhanced function and ergonomics for visualization of ophthalmic surgical maneuvers. Integration of an electrically tunable lens allows rapid focal plane adjustment and iOCT imaging of both anterior and posterior segment tissue microstructures while maintaining parfocality with the ophthalmic surgical microscope. We demonstrate novel visualization of instrument positions relative to tissue layers of interest as colormap overlays onto en face OCT data, which may provide integrative display of volumetric information during surgical maneuvers. Finally, we implement a heads-up display system to provide real-time feedback ...

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    11. Computational adaptive optics for interferometric synthetic aperture microscopy and other interferometric imaging

      Computational adaptive optics for interferometric synthetic aperture microscopy and other interferometric imaging

      Methods for correcting for aberrations in the image or three-dimensional reconstruction of a sampled region obtained by broadband interferometry. The sampled region is illuminated with a broadband beam of light, and light returned from the sample is detected, along with a reference beam, in order to derive an interference signal for pixels of a volume spanned by wavenumber and axes transverse to the beam propagation direction. An optimization procedure is performed with respect to a specified criterion so as to obtain an aberration-corrected image of at least one plane of the sampled region, either in a plane-specific manner or in ...

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    12. Feature Of The Week 5/18/14: Label-free cell-based assay with spectral-domain optical coherence phase microscopy (Narrated Presentation)

      Feature Of The Week 5/18/14: Label-free cell-based assay with spectral-domain optical coherence phase microscopy (Narrated Presentation)

      Quantitative measurement of dynamic responses of unstained living cells is of great importance in many applications ranging from investigation of fundamental cellular functions to drug discoveries. Conventional optical methods for label-free cell-based assay examine cellular structural changes proximal to sensor surfaces under external stimuli, but require dedicated nanostructure-patterned substrates for operation. Here, we present a quantitative imaging method, spectral-domain optical coherence phase microscopy (SD-OCPM), as a viable optical platform for label-free cell-based assay. The instrument is based on a low-coherence interferometric microscope that enables quantitative depth-resolved phase measurement of a transparent specimen with high phase stability. We demonstrate SD-OCPM measurement ...

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    13. Intraoperative optical coherence tomography using the RESCAN 700: preliminary results from the DISCOVER study

      Intraoperative optical coherence tomography using the RESCAN 700: preliminary results from the DISCOVER study

      Significant integrative advances are needed for intraoperative optical coherence tomography ( i OCT) to achieve widespread use across ophthalmic surgery. A surgeon feedback system that provides microscope integration, heads-up display and foot pedal control of the OCT scan location represents a major interval advance in ophthalmic surgery. In this report, we describe the preliminary findings of the Determination of feasibility of Intraoperative Spectral domain microscope Combined/integrated OCT Visualisation during En face Retinal and ophthalmic surgery (DISCOVER) study, a multisurgeon investigational device study examining the role of microscope integrated i OCT systems with surgeon heads-up display feedback (eg, Carl Zeiss Meditec ...

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    14. Cornea characterization using a combined multiphoton microscopy and optical coherence tomography system

      Cornea characterization using a combined multiphoton microscopy and optical coherence tomography system

      We present a multimodal imaging system which combines multiphoton microscopy and optical coherence tomography to visualize the morphological structures, and to quantify the refractive index (RI) and thickness of cornea. The morphological similarities and differences at different corneal layers across various species are identified. In the piscine and human corneas, the stromata exhibit thin fibers that indicate an overall collagen direction. Human corneas display collagen micro-folds which cause increased light attenuation. In the murine, porcine and bovine corneas, the stromata show interwoven collagen patterns. The Bowman’s layer and the Descemet’s membrane are also distinguished in some species. The ...

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    15. Label-free cell-based assay with spectral-domain optical coherence phase microscopy

      Label-free cell-based assay with spectral-domain optical coherence phase microscopy

      Quantitative measurement of dynamic responses of unstained living cells is of great importance in many applications ranging from investigation of fundamental cellular functions to drug discoveries. Conventional optical methods for label-free cell-based assay examine cellular structural changes proximal to sensor surfaces under external stimuli, but require dedicated nanostructure-patterned substrates for operation. Here, we present a quantitative imaging method, spectral-domain optical coherence phase microscopy (SD-OCPM), as a viable optical platform for label-free cell-based assay. The instrument is based on a low-coherence interferometric microscope that enables quantitative depth-resolved phase measurement of a transparent specimen with high phase stability. We demonstrate SD-OCPM measurement ...

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    16. In vivo imaging of middle-ear and inner-ear microstructures of a mouse guided by SD-OCT combined with a surgical microscope

      In vivo imaging of middle-ear and inner-ear microstructures of a mouse guided by SD-OCT combined with a surgical microscope

      We developed an augmented-reality system that combines optical coherence tomography (OCT) with a surgical microscope. By sharing the common optical path in the microscope and OCT, we could simultaneously acquire OCT and microscope views. The system was tested to identify the middle-ear and inner-ear microstructures of a mouse. Considering the probability of clinical application including otorhinolaryngology, diseases such as middle-ear effusion were visualized using in vivo mouse and OCT images simultaneously acquired through the eyepiece of the surgical microscope during surgical manipulation using the proposed system. This system is expected to realize a new practical area of OCT application.

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    17. Low coherence interferometric second harmonic generation microscopy for non-destructive material testing using a broadband 1550 nm fs-fiber laser

      Low coherence interferometric second harmonic generation microscopy for non-destructive material testing using a broadband 1550 nm fs-fiber laser

      In this paper low coherence interferometric second harmonic generation (SHG) imaging is successfully demonstrated using a compact broadband fs-fiber laser operating around 1550 nm. In combination with linear optical coherence microscopy, this SHG technique is tailored for structural and functional investigations of surfaces and subsurface regions of non-biological samples, like corrosion sites formed on metals below organic coatings.

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    18. Simultaneous detection of optical retardation and axis orientation by polarization-sensitive full-field optical coherence microscopy for material testing

      Simultaneous detection of optical retardation and axis orientation by polarization-sensitive full-field optical coherence microscopy for material testing

      We present a polarization-sensitive full-field optical coherence microscopy modality which is capable of simultaneously delivering depth resolved information on the reflectivity, optical retardation and optical axis orientation. In this way local birefringence, inherent stress–strain fields and optical anisotropies can be visualized with high resolution, as exemplified for various technical material applications.

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    19. Label-free evaluation of angiogenic sprouting in microengineered devices using ultrahigh-resolution optical coherence microscopy

      Label-free evaluation of angiogenic sprouting in microengineered devices using ultrahigh-resolution optical coherence microscopy

      Understanding the mechanism of angiogenesis could help to decipher wound healing and embryonic development and to develop better treatment for diseases such as cancer. Microengineered devices were developed to reveal the mechanisms of angiogenesis, but monitoring the angiogenic process nondestructively in these devices is a challenge. In this study, we utilized a label-free imaging technique, ultrahigh-resolution optical coherence microscopy (OCM), to evaluate angiogenic sprouting in a microengineered device. The OCM system was capable of providing ∼ 1.5 - μ m axial resolution and ∼ 2.3 - μ m transverse resolution. Three-dimensional (3-D) distribution of the sprouting vessels in the microengineered device was imaged over ...

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    20. Quantitative cerebral blood flow imaging with extended-focus optical coherence microscopy

      Quantitative cerebral blood flow imaging with extended-focus optical coherence microscopy

      Quantitative three-dimensional blood flow imaging is a valuable technique to investigate the physiology of the brain. Two-photon microscopy (2PM) allows quantification of the local blood flow velocity with micrometric resolution by performing repeated line scans, but prohibitively long measurement times would be required to apply this technique to full three-dimensional volumes. By multiplexing the image acquisition over depth, Fourier domain optical coherence tomography (FDOCT) enables quantification of blood flow velocities with a high volume acquisition rate, albeit at a relatively low spatial resolution. Extended-focus optical coherence microscopy (xfOCM) increases the lateral resolution without sacrificing depth of field and therefore combines ...

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    21. LLTech and Research Partners win a 800K€ grant for FF-OCT NOCT project

      LLTech and Research Partners win a 800K€ grant for FF-OCT NOCT project

      NOCT program is research program who is awarded a 800K€ grant from the ANR (Agence Nationale de la Recherche). The target is to develop a 3D guided needle endoscope system. LLTech collaborates with Institut Langevin, SurgiQual Institute, Université Joseph Fourier and Centre Hospitalier de Grenoble. The current biopsy procedure is to introduce a needle inside the patient towards a given target using echography imaging for control of the position. Reaching the target at the right position is a real issue for diagnosis, therapy and also prognosis, for example concerning tumors or abscess. The NOCT project aims at developing two apparatus ...

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    22. Full-field optical coherence tomography for the analysis of fresh unstained human lobectomy specimens

      Full-field optical coherence tomography for the analysis of fresh unstained human lobectomy specimens

      Full-field optical coherence tomography (FFOCT) is a real-time imaging technique that generates high-resolution three-dimensional tomographic images from unprocessed and unstained tissues. Lack of tissue processing and associated artifacts, along with the ability to generate large-field images quickly, warrants its exploration as an alternative diagnostic tool. Materials and Methods: One section each from the tumor and from adjacent non-neoplastic tissue was collected from 13 human lobectomy specimens. They were imaged fresh with FFOCT and then submitted for routine histopathology. Two blinded pathologists independently rendered diagnoses based on FFOCT images. Results: Normal lung architecture (alveoli, bronchi, pleura and blood vessels) was readily ...

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    23. Methods, systems, and computer readable media for synthetic wavelength-based phase unwrapping in optical coherence tomography and spectral domain phase microscopy

      Methods, systems, and computer readable media for synthetic wavelength-based phase unwrapping in optical coherence tomography and spectral domain phase microscopy

      In accordance with this disclosure, methods, systems, and computer readable media for synthetic wavelength-based phase unwrapping in optical coherence tomography are provided. Synthetic wavelength phase unwrapping can be applied to OCT data and can correctly resolve sample motions that are larger than .lamda..sub.o/2. A method for phase unwrapping of an OCT signal can include acquiring raw OCT signal data, interpolating and processing the OCT signal data to obtain a DC spectrum, comparing the raw OCT signal data to the DC spectrum to generate an interference signal, applying Gaussian windows to the interference signal to generate a two ...

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    24. Three-dimensional optical coherence tomography confocal imaging apparatus

      Three-dimensional optical coherence tomography confocal imaging apparatus

      A 3D OCT confocal imaging apparatus includes a light source module for providing an illumination beam with wider bandwidth from a crystal fiber; a reference source module; a pickup module; a beam splitter; an optical filter; and a sensor module. When the illumination beam illuminates a sample, a pickup objective lens and a piezoelectric actuator of the pickup module together provide an image beam scanning the sample in depth direction. The image beam and a reference beam from the reference source module together form an interference image beam, which is converted by a photosensor into a coherence image electric signal ...

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    1-24 of 376 1 2 3 4 ... 14 15 16 »
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