Microscopy -Optical Coherence Tomography News

Articles in category: Microscopy

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1. Chain-like gold nanoparticle clusters for multimodal photoacoustic microscopy and optical coherence tomography enhanced molecular imaging
  
  Explore Nature Publishing Group Jan 6 2021 Microscopy
  
  Colloidal gold nanoparticles (GNPs) serve as promising contrast agents in photoacoustic (PA) imaging, yet their utility is limited due to their absorption peak in the visible window overlapping with that of hemoglobin. To overcome such limitation, this report describes an ultrapure chain-like gold nanoparticle (CGNP) clusters with a redshift peak wavelength at 650 nm. The synthesized CGNP show an excellent biocompatibility and photostability. These nanoparticles are conjugated with arginine-glycine-aspartic acid (RGD) peptides (CGNP clusters-RGD) and validated in 12 living rabbits to perform multimodal photoacoustic microscopy (PAM) and optical coherence tomography (OCT) for visualization of newly developed blood vessels in the ...
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  Mentions: University of Michigan
2. Indentation probe with optical fiber array-based optical coherence tomography for material deformation
  
  Explore Wiley Online Library Dec 16 2020 Microscopy
  
  We present a new optomechanical probe for mechanical testing of soft matter. The probe consists of a micromachined cantilever equipped with an indenting sphere, and an array of 16 single‐mode optical fibers, which are connected to an Optical Coherence Tomography (OCT) system that allows sub‐surface analysis of the sample during the indentation stroke. To test our device and its capability, we performed indentation on a PDMS‐based phantom. Our findings demonstrate that Common Path (CP)‐OCT via lensed optical fibers can be successfully combined with a microindentation sensor to visualize the phantom's deformation profile at different indentation ...
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3. Laser scanning reflection-matrix microscopy sees inside intact mouse skull
  
  Explore optics.org Dec 8 2020 Microscopy
  
  Visualizing tissues and neurons inside an animal's skull has long been a challenge for biophotonics techniques, thanks to the skull's thickness and the complex structures inside. Approaches to this problem have included the use of endoscopic platforms in brain imaging, two-photon and three-photon microscopy of small animals, and for surgical purposes the creation of a transparent window in the skull itself. A team at the Institute for Basic Science ( IBS ) in Daejeon, South Korea, has now developed a new approach to tackling the optical aberrations involved in imaging through an intact skull, and tested the system on a ...
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4. Optical comparison between micro-CT and OCT in imaging of marginal composite adaptation; Observational study
  
  Explore Wiley Online Library Nov 29 2020 Microscopy
  
  Dental composite is the most used aesthetic restorative biomaterial worldwide. However, it undergoes polymerization shrinkage that could lead to loss of the interfacial seal between tooth and resin in some circumstances. This demands high-resolution imaging technologies to detect these defects. This study carried out a comparison between microcomputed tomography (micro-CT; Shimadzu, Japan) and swept-source optical coherence tomography (SS-OCT; Santec, Japan) in the detection of marginal adaptation defects at the tooth-resin interface. Unlike in micro-CT, it was possible to outline interfacial gaps along with tooth-resin interfaces with SS-OCT, which was attributed to the Fresnel diffraction of light. This in-vitro comparison demonstrates ...
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  Mentions: Santec Turki Bakhsh King Abdullah University of Science and Technology
5. Multi-directional Morphological Assessment of Single Bacterial Colonies Through Non-invasive Optical Imaging
  
  Explore Home - Springer Oct 31 2020 Microscopy
  
  A bacterium in bacterial colony is a basal component of bacterial studies and is therefore of considerable importance. The morphological characteristics of a single colony have been widely used as the standard to identify diverse bacterial species. However, the conventional methods for obtaining morphological information, such as microscopic techniques, cannot provide tomographic views. This study utilizes spectral-domain optical coherence tomography (SD-OCT) to observe both external and internal structures for single colonies. OCT imaging provided a clear top (projection) and lateral (cross-sectional) images, which can identify distinct features in the fifteen different bacterial species examined. Through the acquired OCT images, the ...
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  Mentions: Jeehyun Kim Kyungpook National University Ruchire Eranga Wijesinghe
6. Visualization of Bacterial Colonization and Cellular Layers in a Gut-on-a-Chip System Using Optical Coherence Tomography
  
  Explore Cambridge University Press Oct 28 2020 Gastroenterology , Microscopy
  
  Imaging of cellular layers in a gut-on-a-chip system has been confined to two-dimensional (2D)-imaging through conventional light microscopy and confocal laser scanning microscopy (CLSM) yielding three-dimensional- and 2D-cross-sectional reconstructions. However, CLSM requires staining and is unsuitable for longitudinal visualization. Here, we compare merits of optical coherence tomography (OCT) with those of CLSM and light microscopy for visualization of intestinal epithelial layers during protection by a probiotic Bifidobacterium breve strain and a simultaneous pathogen challenge by an Escherichia coli strain. OCT cross-sectional images yielded film thicknesses that coincided with end-point thicknesses derived from cross-sectional CLSM images. Light microscopy on histological ...
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  Mentions: University of Groningen
7. Optical coherence tomography microscopy in experimental traumatic brain injury
  
  Explore Wiley Online Library Oct 5 2020 Microscopy , Neurology
  
  Worldwide elderly traumatic brain injury (TBI) patients tend to become an increasing burden to the society. Thus, a faster and less expensive way of evaluating TBI victims is needed. In the present study we investigated if optical coherence tomography (OCT) could be used as such a method. By using an animal model, we established if OCT can detect cortical changes in the acute phase of a penetrating TBI, in young (5-7 months) and old (20-22 months) rats. Due to the long-term evolution of TBI's, we wanted to investigate to what extent OCT could detect changes within the cortex in ...
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8. Morphological, optical, and elemental analysis of dental enamel after debonding laminate veneer with Er,Cr:YSGG laser: A pilot study
  
  Explore Wiley Online Library Sep 28 2020 Dentistry , Microscopy
  
  Laminate veneer removal is becoming a routine procedure at the dental clinic and the use of laser can facilitate its removal. This work aimed to evaluate the morphological, elemental, and optical changes in the remaining enamel after veneer removal using Er,Cr:YSGG laser. Forty‐four enamel slabs were prepared and randomly distributed into nine experimental groups, for bonding using lithium disilicate laminates with three different luting agents (Variolink Veneer, RelyX U200, and RelyX Veneer). Then each agent was debonded using Er,Cr:YSGG laser (2.78 μm) using two different protocols:3.5 W, 48.14 J/cm 2 ...
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9. High Resolution Multimodal Photoacoustic Microscopy and Optical Coherence Tomography Visualization of Choroidal Vascular Occlusion
  
  Explore MDPI Open Access Journals Platform Sep 5 2020 Microscopy , Ophthalmology
  
  Photoacoustic microscopy is a novel, non-ionizing, non-invasive imaging technology that evaluates tissue absorption of short-pulsed light through the sound waves emitted by the tissue and has numerous biomedical applications. In this study, a custom-built multimodal imaging system, including photoacoustic microscopy (PAM) and optical coherence tomography (OCT), has been developed to evaluate choroidal vascular occlusion (CVO). CVO was performed on three living rabbits using laser photocoagulation. Longitudinal imaging of CVO was obtained using multiple imaging tools such as color fundus photography, fluorescein angiography, indocyanine green angiography (ICGA), OCT, and PAM. PAM images were acquired at different wavelengths, ranging from 532 to ...
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  Mentions: University of Michigan
10. Role of Microscope-Intraoperative Optical Coherence Tomography in paediatric keratoplasty: A comparative Study
  
  Explore American Journal of Ophthalmology Aug 7 2020 Microscopy , Ophthalmology
  
  Purpose To evaluate role of Microscope-Integrated Intraoperative Optical Coherence Tomography (i-OCT) in paediatric keratoplasty. Design Combined prospective and retrospective, comparative, interventional study Setting Dr Rajendra Prasad Centre for Ophthalmic Sciences, India Methods Seventy-five children aged ≤16 years planned for keratoplasty were divided into two groups and compared for intraoperative course and postoperative outcome. In Group-1 (prospective group, n=56), pre-operative anterior segment visualization was performed clinically, with ultrasound biomicroscopy (UBM) and i-OCT and keratoplasty was commenced under i-OCT microscope. In Group-2 (retrospective group, n=19), conventional microscope was employed during keratoplasty. Results In Group-1, i-OCT, UBM and clinical examination detected ...
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11. Laser Lens Size Measurement Using Swept-Source Optical Coherence Tomography
  
  Explore MDPI Open Access Journals Platform Jul 17 2020 Microscopy , NDE/NDT
  
  A high-speed, high-resolution swept-source optical coherence tomography (SS-OCT) is presented for focusing lens imaging and a k-domain uniform algorithm is adopted to find the wave number phase equalization. The radius of curvature of the laser focusing lens was obtained using a curve-fitting algorithm. The experimental results demonstrate that the measuring accuracy of the proposed SS-OCT system is higher than the laser confocal microscope.
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12. What Is the Impact of Intraoperative Microscope-Integrated OCT in Ophthalmic Surgery? Relevant Applications and Outcomes. A Systematic Review
  
  Explore MDPI Open Access Journals Platform Jun 2 2020 Microscopy , Ophthalmology
  
  Background: Optical coherence tomography (OCT) has recently been introduced in the operating theatre. The aim of this review is to present the actual role of microscope-integrated optical coherence tomography (MI-OCT) in ophthalmology. Method: A total of 314 studies were identified, following a literature search adhering to the preferred reporting items for systematic reviews and meta-analyses (PRISMA) guidelines. After full-text evaluation, 81 studies discussing MI-OCT applications in ophthalmology were included. Results: At present, three microscope-integrated optical coherence tomography systems are commercially available. MI-OCT can help anterior and posterior segment surgeons in the decision-making process, providing direct visualization of anatomic planes before ...
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13. Light detection and ranging system with photonic integrated circuit
  
  Explore uspto.gov Apr 21 2020 Microscopy , Patents
  
  Disclosed herein are optical integration technologies, designs, systems and methods directed toward Optical Coherence Tomography (OCT) and other interferometric optical sensor, ranging, and imaging systems wherein such systems, methods and structures employ tunable optical sources, coherent detection and other structures on a single or multichip monolithic integration. In contrast to contemporary, prior-art OCT systems and structures that employ simple, miniature optical bench technology using small optical components positioned on a substrate, systems and methods according to the present disclosure employ one or more photonic integrated circuits (PICs), use swept-source techniques, and employ a widely tunable optical source(s). In another ...
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  Mentions: Eric A. Swanson
14. Vectorial birefringence imaging by optical coherence microscopy for assessing fibrillar microstructures in the cornea and limbus
  
  Explore OSA | OSA Publishing Jan 25 2020 Microscopy
  
  The organization of fibrillar tissue on the micrometer scale carries direct implications for health and disease but remains difficult to assess in vivo . Polarization-sensitive optical coherence tomography measures birefringence, which relates to the microscopic arrangement of fibrillar tissue components. Here, we demonstrate a critical improvement in leveraging this contrast mechanism by employing the improved spatial resolution of focus-extended optical coherence microscopy (1.4 µm axially in air and 1.6 µm laterally, over more than 70 µm depth of field). Vectorial birefringence imaging of sheep cornea ex vivo reveals its lamellar organization into thin sections with distinct local optic axis ...
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  Mentions: Massachusetts General Hospital Harvard University David D. Sampson
15. Computed optical coherence microscopy of mouse brain ex vivo
  
  Explore SPIE Digital Library Dec 1 2019 Microscopy , Neurology
  
  The compromise between lateral resolution and usable imaging depth range is a bottleneck for optical coherence tomography (OCT). Existing solutions for optical coherence microscopy (OCM) suffer from either large data size and long acquisition time or a nonideal point spread function. We present volumetric OCM of mouse brain ex vivo with a large depth coverage by leveraging computational adaptive optics (CAO) to significantly reduce the number of OCM volumes that need to be acquired with a Gaussian beam focused at different depths. We demonstrate volumetric reconstruction of ex-vivo mouse brain with lateral resolution of 2.2 μm, axial resolution of ...
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  Mentions: Cornell University Steven G. Adie
16. Microscope having an optical coherence tomography device
  
  Explore uspto.gov Nov 12 2019 Microscopy , Patents
  
  A microscope having an observation beam path including a main objective, an OCT device including a first detection beam path, a wavefront measuring device including a second detection beam path, a first, a second and a third optics group is provided, wherein the first detection beam path contains the main objective and the first to third optics group, and the first to third optics group forms an afocal imaging optical unit of the first detection beam path and the second detection beam path contains the main objective, the first optics group and the second optics group, and the main objective ...
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  Mentions: Carl Zeiss Meditec
17. Combination of 2D Compressive Sensing Spectral Domain Optical Coherence Tomography and Interferometric Synthetic Aperture Microscopy
  
  Explore MDPI Open Access Journals Platform Sep 25 2019 Microscopy
  
  Combining the advantages of compressive sensing spectral domain optical coherence tomography (CS-SDOCT) and interferometric synthetic aperture microscopy (ISAM) in terms of data volume, imaging speed, and lateral resolution, we demonstrated how compressive sampling and ISAM can be simultaneously used to reconstruct an optical coherence tomography (OCT) image. Specifically, an OCT image is reconstructed from two-dimensional (2D) under-sampled spectral data dimension-by-dimension through a CS reconstruction algorithm. During the iterative process of CS algorithm, the deterioration of lateral resolution beyond the depth of focus (DOF) of a Gaussian beam is corrected. In the end, with less spectral data, we can obtain an ...
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  Mentions: Tsinghua University
18. VIS-OCT offers sight line
  
  Explore Photonics.com Sep 19 2019 Microscopy , Ophthalmology
  
  The benefits of optical coherence tomography (OCT) have long been known as an imaging tool in ophthalmology, enabling the examination of the eye at microscopic resolution. But what may not be widely appreciated is that the use of visible light sources can improve upon the results of this well-developed technique in the detection and understanding of various eye-related diseases such as macular degeneration and glaucoma. While most commercial OCT and OCTA (angiography) systems use NIR light for economic and technical reasons, broadband visible light sources have expanded from 400 to 2000 nm or more. This has enabled the imaging of ...
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  Mentions: Northwestern University Hao F. Zhang Opticent Health
19. IMAGING TECHNIQUE FROM BECKMAN LAB NAMED TOP 10 MICROSCOPY INNOVATION
  
  Explore beckman.illinois.edu Aug 21 2019 Microscopy
  
  n imaging technique developed at the Biophotonics Imaging Laboratory at the Beckman Institute was named one of the 10 best microscopy innovations of 2019 by Microscopy Today, the official magazine of the Microscopy Society of America. Dr. Stephen Boppart, a professor of bioengineering and of electrical and computer engineering Simultaneous label-free autofluorescence multi-harmonic (SLAM) microscopy is a new imaging technology developed by senior research scientist Haohua Tu, bioengineering graduate student Sixian You, and a team of researchers at the Biophotonics Imaging Laboratory, led by Dr. Stephen Boppart, a professor of bioengineering and of electrical and computer engineering at Illinois, and ...
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  Mentions: Stephen A. Boppart University of Illinois at Urbana-Champaign Haohua Tu
20. Ophthalmic operation microscope
  
  Explore uspto.gov Aug 20 2019 Microscopy , Ophthalmology , Patents
  
  In an ophthalmic operation microscope, an illumination optical system illuminates a patient's eye with illumination light. An observation optical system is used for observing the patient's eye illuminated. An objective lens is disposed in an observation optical path. An interference optical system splits light from a light source into measurement light and reference light, and detects interference light generated from returning light of the measurement light from the patient's eye and the reference light. A first lens group is disposed between the light source and the patient's eye in an optical path of the measurement light ...
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  Mentions: Topcon Medical Systems
21. In vivo corneal confocal microscopy aided by optical coherence tomography
  
  Explore OSA | OSA Publishing Apr 26 2019 Microscopy , Ophthalmology
  
  In vivo corneal confocal microscopy and its operability in scientific as well as in clinical applications is often impaired by the lack of information on imaging plane position and orientation inside the cornea during patient’s examination. To overcome this hurdle, we have developed a novel corneal imaging system based on a commercial scanning device and a modified Rostock Cornea Module. The presented preliminary system produces en face images by confocal laser scanning microscopy and sagittal cross-section images by optical coherence tomography simultaneously. This enables imaging guidance during examinations, improved features for diagnostics along with thickness measurements of the cornea ...
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  Mentions: Universität Rostock
22. En face optical coherence tomography: a technology review [Invited]
  
  Explore OSA | OSA Publishing Apr 9 2019 Microscopy
  
  A review on the technological development of en face optical coherence tomography (OCT) and optical coherence microscopy (OCM) is provided. The terminology originally referred to time domain OCT, where the preferential scanning was performed in the en face plane. Potentially the fastest realization of en face image recording is full-field OCT, where the full en face plane is illuminated and recorded simultaneously. The term has nowadays been adopted for high-speed Fourier domain approaches, where the en face image is reconstructed from full 3D volumes either by direct slicing or through axial projection in post processing. The success of modern en ...
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  Mentions: Rainer A. Leitgeb Medical University of Vienna
23. Stereoscopic display systems and methods for displaying surgical data and information in a surgical microscope
  
  Explore uspto.gov Mar 26 2019 Microscopy , Patents
  
  Stereoscopic display systems and methods for displaying surgical data and information in a surgical microscope are disclosed herein. According to an aspect, a system includes first and second eyepieces. The system includes a display having first and second display portions, configured to display first images in the first display portion, and configured to display second images in the second display portion. The first image and the second image are projected along a first pathway and a second pathway. The system includes a first optical element positioned to relay the first images into the first eyepiece. The system includes a second ...
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  Mentions: Joseph A. Izatt Duke University Cynthia A. Toth
24. Optical coherence tomography microscopy apparatus and method for detecting a three-dimensional image of an object
  
  Explore uspto.gov Feb 26 2019 Microscopy , Patents
  
  An optical coherence tomography microscopy apparatus (1) is presented for detecting a three-dimensional image of an optically translucent or reflective sample object (OS), the apparatus comprising an interferometric optical setup including a photo sensor unit (20). A sense signal Si from the photo sensor unit (20) is detected using a detection reference signal. The detection reference signal is derived from a signal indicative for a relative displacement of the sample object (OS) with respect to a reference object.
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