1. Articles in category: Microscopy

    1-24 of 443 1 2 3 4 ... 17 18 19 »
    1. Optical coherence microscopy with extended focus for in vivo embryonic imaging

      Optical coherence microscopy with extended focus for in vivo embryonic imaging

      Optical coherence microscopy (OCM) has unique advantages of high-resolution volumetric imaging without relying on exogenous labels or dyes. It combines the coherence-gated depth discrimination of optical coherence tomography (OCT) with the high lateral resolution of confocal microscopy, offering an excellent balance between the resolutions and imaging depth. However, as the lateral resolution becomes higher, the imaging depth of OCM decreases and its three-dimensional imaging capability is greatly degraded. To overcome this limitation, we used amplitude apodization to create quasi-Bessel beam illumination in order to extend the depth of focus. The lateral and axial resolutions of our OCM system were measured ...

      Read Full Article
    2. Optical coherence tomography technique

      Optical coherence tomography technique

      An optical coherence tomography device comprises a light generator, a dispersive medium, an optical coupler and a detector. The light generator is adapted to generate a series of input pulses of coherent light, each input pulse having an input pulse width. The dispersive medium has an input that is optically coupled to the light generator and an output for output pulses. The dispersive medium is adapted to stretch the input pulse width to an output pulse width of each of the output pulses by chromatic dispersion. The optical coupler is adapted to couple the output pulses into a reference arm ...

      Read Full Article
      Mentions: Henning Wisweh
    3. Measurement of dynamic cell-induced 3D displacement fields in vitro for traction force optical coherence microscopy

      Measurement of dynamic cell-induced 3D displacement fields in vitro for traction force optical coherence microscopy

      Traction force microscopy (TFM) is a method used to study the forces exerted by cells as they sense and interact with their environment. Cell forces play a role in processes that take place over a wide range of spatiotemporal scales, and so it is desirable that TFM makes use of imaging modalities that can effectively capture the dynamics associated with these processes. To date, confocal microscopy has been the imaging modality of choice to perform TFM in 3D settings, although multiple factors limit its spatiotemporal coverage. We propose traction force optical coherence microscopy (TF-OCM) as a novel technique that may ...

      Read Full Article
    4. Articular Cartilage Surface Topography and Roughness in Frozen Tissue Samples Using Optical Coherence Microscopy

      Articular Cartilage Surface Topography and Roughness in Frozen Tissue Samples Using Optical Coherence Microscopy

      This paper describes a method to visualize the topography of the surface of cartilage. Optical Coherence Microscopy (OCM) technique has been used to get two dimensional images of frozen tissue samples of bovine articular cartilage. Optical Coherence Microscopy (OCM) is a combination of optical coherence tomography and confocal microscopy. Coherence gate from OCT and confocal gate from confocal microscopy can achieve higher resolution and deeper penetration depth. The precise three-dimensional topography of the cartilage surfaces has been obtained. The full-image roughness, for frozen samples has been obtained.

      Read Full Article
    5. Microscope-less wide field-of-view surgical OCT visualization system

      Microscope-less wide field-of-view surgical OCT visualization system

      A surgical imaging system can comprise a light source, configured to generate an imaging light beam; a beam guidance system, configured to guide the imaging light beam from the light source; a beam scanner, configured to receive the imaging light from the beam guidance system, and to generate a scanned imaging light beam; a beam coupler, configured to redirect the scanned imaging light beam; and a wide field of view (WFOV) lens, configured to guide the redirected scanned imaging light beam into a target region of a procedure eye.

      Read Full Article
      Mentions: Lingfeng Yu
    6. En face coherence microscopy [Invited]

      En face coherence microscopy [Invited]

      En face coherence microscopy or flying spot or full field optical coherence tomography or microscopy (FF-OCT/FF-OCM) belongs to the OCT family because the sectioning ability is mostly linked to the source coherence length. In this article we will focus our attention on the advantages and the drawbacks of the following approaches: en face versus B scan tomography in terms of resolution, coherent versus incoherent illumination and influence of aberrations, and scanning versus full field imaging. We then show some examples to illustrate the diverse applications of en face coherent microscopy and show that endogenous or exogenous contrasts can add ...

      Read Full Article
    7. Postdoctoral Research Fellowships in In Vivo Microscopy at Massachusetts General Hosptial

      Postdoctoral Research Fellowships in In Vivo Microscopy at Massachusetts General Hosptial

      Employer Description Until recently, visualizing the architectural and cellular morphology of human tissue has required histopathological examination. Samples would be excised from the patient, processed, sectioned, stained and viewed under a microscope. In addition to being invasive, time consuming and costly, the static nature of conventional pathology prohibits the study of biological dynamics and function. The Tearney Laboratory at Massachusetts General Hospital has led the way in transforming the current diagnostic paradigm through the invention and translation of new noninvasive, high-resolution optical imaging modalities that enable disease diagnosis from living patients without excising tissues from the body. Led by Guillermo ...

      Read Full Article
    8. Introducing 3-D OCT to live surgery

      Introducing 3-D OCT to live surgery

      No doubt the commercialization of optical coherence tomography (OCT) has forever changed the clinical management of retinal disorders. Its introduction into the surgical realm may be a game changer in its own right, said Pravin U. Dugel, MD. Modern microscopes limit the surgeon’s view, and “axial information must be inferred from instrument shadowing and other indirect cues.” 1 As advances in OCT technology have increased the ability of these devices to image ocular structures better, the category as a whole has moved into a wide variety of applications, including the ability to assess subretinal fluid, pigment epithelial detachments, and ...

      Read Full Article
    9. Imaging Microscopic Features of Keratoconic Corneal Morphology

      Imaging Microscopic Features of Keratoconic Corneal Morphology

      Purpose: To search for gold-standard histology indicators using alternative imaging modalities in keratoconic corneas. Methods: Prospective observational case–control study. Fourteen keratoconic corneas and 20 normal corneas (10 in vivo healthy subjects and 10 ex vivo donor corneas) were examined. Images of corneas were taken by spectral domain optical coherence tomography (SD-OCT) and in vivo confocal microscopy (IVCM) before keratoplasty. The same removed corneal buttons were imaged after keratoplasty with full-field optical coherence microscopy (FFOCM) and then fixed and sent for histology. Controls consisted of normal subjects imaged in vivo with IVCM and donor corneas imaged ex vivo with FFOCM ...

      Read Full Article
    10. Using light to map the circuitry of the brain

      Using light to map the circuitry of the brain

      Lehigh University bio-engineers are first to explore the use of highly-sensitive, non-invasive optical coherence tomography (OCT) to examine neuron-to-neuron communication in live tissue. Scientific progress has provided a solid understanding of the anatomy of the brain. However, there is still no reliable way to examine neuron to neuron communication, as it happens--a key to understanding the correlation between brain structure and brain function. Chao Zhou , assistant professor of bioengineering at Lehigh University, likens our current brain-mapping ability to a Global Positioning System (GPS) that can help a user locate a city, but cannot offer a street-level view. With current imaging ...

      Read Full Article
    11. Intracellular dynamics measurements with full field optical coherence tomography suggest hindering effect of actomyosin contractility on organelle transport

      Intracellular dynamics measurements with full field optical coherence tomography suggest hindering effect of actomyosin contractility on organelle transport

      Intracellular motion can be quantitatively monitored in tissues using coherence-gated microscopic techniques. With full-field optical coherence tomography (FFOCT), the use of high numerical aperture microscope objectives provides a high resolution mapping of intracellular dynamics that are probed with subwavelength sensitivity. In the upper temporal bandwidth that we have used (1-6 Hz) the main contribution to the dynamic signal arises from the overall dynamical, optically heterogeneous cytoplasm. We propose a method to specifically study the impact of actomyosin contractility on the intracellular dynamic signal by performing high throughput, comparative measurements of multicellular aggregates with and without blebbistatin action, a selective inhibitor ...

      Read Full Article
    12. Method and apparatus for automated scanning probe microscopy

      Method and apparatus for automated scanning probe microscopy

      This invention concerns scanning probe microscopes and related instruments ("SPMs") when used to investigate or measure large samples whose size is a multiple of the typical operational scanning area of an SPM, say 150 .mu.m.times.150 .mu.m at most. To avoid frequent readjustments or other time-consuming human interaction and errors when focusing the SPM, a multi-step, automated method for the SPM-scanning of large samples is disclosed, comprising a "coarse", i.e. low resolution, non-SPM scanning or mapping step adapted to scan a large sample and providing an integral map of the sample, followed by a preferably mathematical ...

      Read Full Article
    13. Optical measurement apparatus and optical measurement method

      Optical measurement apparatus and optical measurement method

      A small and inexpensive optical measurement apparatus is provided in which noise due to optical interference such as inter-layer crosstalk or speckle is suppressed. The optical measurement apparatus includes a light source to emit laser light, a high-frequency superimposing unit to superimpose a high-frequency current on a driving current to drive the light source, a light branching element to branch the laser light into signal light and reference light, an objective lens to condense and irradiate the signal light on a measurement object, a condensing position scanning unit to scan a condensing position of the signal light, a light path ...

      Read Full Article
    14. Optical coherence photoacoustic microscopy

      Optical coherence photoacoustic microscopy

      A system and method for providing an optical coherence photoacoustic (OC-PAM) microscopy. An OC-PAM microscope includes a light source that outputs light, a scanner, a detector, a transducer, and an image processing module. The scanner receives the light and scans the light across a sample. The detector receives reflected light from the sample in response to the scanned light. The transducer detects photoacoustic waves induced in the sample by the scanned light. The image processing module receives output from the detector and the transducer and generates a photoacoustic microscopy (PAM) image and an optical coherence tomography (OCT) image based on ...

      Read Full Article
    15. Optical Coherence Tomography for Skin Cancer

      Optical Coherence Tomography for Skin Cancer

      Skin cancer is the most common malignancy worldwide, and non-melanoma skin cancers (NMSC) in particular, including basal cell carcinomas (BCC) and squamous cell carcinomas, constitute around 80 percent of all skin cancers. It is estimated that approximately 3.5 million new cases of NMSC occur every year in the U.S., exceeding that of all other cancers combined. Despite the high prevalence of NMSC, they can be effectively treated and cured with low recurrence when detected at an early stage. BCCs are known to be slow growing and rarely metastasize to other parts of the body; however, if left untreated ...

      Read Full Article
    16. Dermoscopy, confocal microscopy and optical coherence tomography for the diagnosis of bedbug infestation

      Dermoscopy, confocal microscopy and optical coherence tomography for the diagnosis of bedbug infestation

      Letter to the editor: A 33 year-old woman suffering from rosacea presented with outbreaks of itchy plaques on her face and limbs that curiously improved when she was far from home during summer holidays (Fig. 1). Dermoscopy (FotoFinder Systems GmbH, Bad Birnbach, Germany) showed petechiae (Fig. 2A) and reflectance confocal microscopy (RCM; Vivascope 3000 ® , Caliber, New York, USA, distributed in Europe by MAVIG GmbH, München, Germany) showed intraepidermal vesicles (Fig. 2B) suggesting parasite bites. RCM found Demodex folliculorum (Fig. 2C) that could have been responsible for rosacea. However, a careful examination with dermoscopy identified a brown oval-shaped wingless insect ...

      Read Full Article
      Mentions: AGFA Healthcare
    17. Velocity gradients in spatially resolved laser Doppler flowmetry and dynamic light scattering with confocal and coherence gating

      Velocity gradients in spatially resolved laser Doppler flowmetry and dynamic light scattering with confocal and coherence gating

      Dynamic light scattering (DLS) is widely used to characterize diffusive motion to obtain precise information on colloidal suspensions by calculating the autocorrelation function of the signal from a heterodyne optical system. DLS can also be used to determine the flow velocity field in systems that exhibit mass transport by incorporating the effects of the deterministic motion of scatterers on the autocorrelation function, a technique commonly known as laser Doppler flowmetry. DLS measurements can be localized with confocal and coherence gating techniques such as confocal microscopy and optical coherence tomography, thereby enabling the determination of the spatially resolved velocity field in ...

      Read Full Article
    18. Heads up displays for optical coherence tomography integrated surgical microscopes

      Heads up displays for optical coherence tomography integrated surgical microscopes

      Surgical microscope systems are provided including an optical coherence tomography (OCT) system; an objective lens; oculars for direct viewing of a subject distal to the objective lens; a heads up display module configured to direct an optical image through the oculars to be visible to a user of at least one ocular; and a coupling element connected to the surgical microscope coupling the OCT system, the heads up display module and the objective lens. The coupling element has first and second faces, the first face positioned toward the oculars of the surgical microscope and the second face positioned toward the ...

      Read Full Article
    19. Your Upgrade Path to High Performance Intrasurgical OCT EnFocus™

      Your Upgrade Path to High Performance Intrasurgical OCT EnFocus™

      EnFocus is an intrasurgical OCT upgrade solution for new and existing surgical microscopes. See the details you are looking for with high-resolution, wide scan, and deep depth OCT images, providing subsurface visualization during surgery. Affordably add intrasurgical OCT directly into your surgical workflow for both anterior and posterior surgery. The EnFocus OCT is attached or removed within minutes; the modular design allows flexible sharing between multiple microscopes in your operating theater

      Read Full Article
      Mentions: Bioptigen Leica
    20. Bimodal endoscopic probe combining white-light microscopy and optical coherence tomography

      Bimodal endoscopic probe combining white-light microscopy and optical coherence tomography

      We present a novel bimodal endoscopic imaging probe that can simultaneously provide full-field white-light video microscopy and confocal optical coherence tomography (OCT) depth scans. The two modalities rely on spectrally separated optical paths that run partially in parallel through a micro-optical bench system, which has a cross-section of only 2    mm × 2.76    mm and is realized via standard silicon micromachining techniques. With a numerical aperture of 0.061, the video modality has a resolution and field of view of 9.3 and 1240    μm × 1080    μm , respectively. The resolution is limited by the pixel spacing of the coherent fiber ...

      Read Full Article
    21. Measuring refractive index and volume of liquid under high pressure with optical coherence tomography and light microscopy

      Measuring refractive index and volume of liquid under high pressure with optical coherence tomography and light microscopy

      Measuring the refractive index and volume of liquid under high pressure simultaneously is a big challenge. This paper proposed an alternative solution by combing optical coherence tomography with microscopy. An experiment for a feasibility study was carried out on polydimethylsiloxane liquid in a diamond anvil cell. The refractive index of the sample increased dramatically with pressure loaded, and the curve of pressure volume was also obtained.

      Read Full Article
    22. EnFocus™ Surgical Microscope from Leica

      EnFocus™  Surgical Microscope from Leica

      EnFocus is an intrasurgical OCT upgrade solution for new and existing surgical microscopes. See the details you are looking for with high-resolution, wide scan, and deep depth OCT images, providing subsurface visualization during surgery. Affordably add intrasurgical OCT directly into your surgical workflow for both anterior and posterior surgery. The EnFocus OCT is attached or removed within minutes; the modular design allows flexible sharing between multiple microscopes in your operating theater.

      Read Full Article
      Mentions: Leica
    1-24 of 443 1 2 3 4 ... 17 18 19 »
  1. Categories

    1. Applications:

      Art, Cardiology, Dentistry, Dermatology, Developmental Biology, Gastroenterology, Gynecology, Microscopy, NDE/NDT, Neurology, Oncology, Ophthalmology, Other Non-Medical, Otolaryngology, Pulmonology, Urology
    2. Business News:

      Acquisition, Clinical Trials, Funding, Other Business News, Partnership, Patents
    3. Technology:

      Broadband Sources, Probes, Tunable Sources
    4. Miscellaneous:

      Jobs & Studentships, Student Theses, Textbooks
  2. Popular Articles

  3. Organizations in the News

    1. (1 articles) Cornell University
    2. (1 articles) University of Southern California
  4. People in the News

    1. (1 articles) Lingfeng Yu
    2. (1 articles) Scott E. Fraser
    3. (1 articles) Steven G. Adie
    4. (1 articles) Yu Chen
    5. (1 articles) Henning Wisweh