1. Articles in category: Microscopy

    1-24 of 414 1 2 3 4 ... 16 17 18 »
    1. Classifying murine glomerulonephritis using optical coherence tomography and optical coherence elastography

      Classifying murine glomerulonephritis using optical coherence tomography and optical coherence elastography

      Elastic-wave propagation in mouse healthy and nephritic kidneys. Acute glomerulonephritis caused by antiglomerular basement membrane marked by high mortality. The primary reason for this is delayed diagnosis via blood examination, urine analysis, tissue biopsy, or ultrasound and X-ray computed tomography imaging. Blood, urine, and tissue-based diagnoses can be time consuming, while ultrasound and CT imaging have relatively low spatial resolution, with reduced sensitivity. Optical coherence tomography is a noninvasive and high-resolution imaging technique that provides superior spatial resolution (micrometer scale) as compared to ultrasound and CT. Changes in tissue properties can be detected based on the optical metrics analyzed from ...

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    2. Preliminary study of optical coherence tomography imaging to identify microscopic extrathyroidal extension in patients with papillary thyroid carcinoma

      Preliminary study of optical coherence tomography imaging to identify microscopic extrathyroidal extension in patients with papillary thyroid carcinoma

      Background and Objectives We evaluated the feasibility of using optical coherence tomography (OCT), to identify microscopic extrathyroidal extension (mETE) in ex vivo thyroidectomy specimens of patients who underwent thyroidectomy for the treatment of papillary thyroid carcinoma (PTC). Methods A total of 170 ex vivo OCT images of the tumor, were acquired just after completion of thyroidectomy in 17 patients. The OCT images of each patient were separately evaluated by two blinded investigators, and the outcomes were compared with the histopathology reports. Results The sensitivity and specificity of mETE identification from the OCT images were 81.4% and 86.0%, respectively ...

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    3. Full-field optical coherence microscopy with optimized ultrahigh spatial resolution

      Full-field optical coherence microscopy with optimized ultrahigh spatial resolution

      Full-field optical coherence microscopy (FF-OCM) with isotropic spatial resolution of 0.5 μm (in water), at 700 nm center wavelength, is reported. A theoretical study of the FF-OCM axial response is carried out for maximizing the axial resolution of the system, considering the effect of optical dispersion. The lateral resolution is optimized by using water-immersion microscope objectives with a numerical aperture of 1.2. This ultrahigh-resolution FF-OCM system is applied to animal and human skin tissue imaging, revealing ultra-fine in-depth structures at the sub-cellular level.

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      Mentions: Arnaud Dubois
    4. Optical tissue sectioning using full field optical coherence tomography

      Optical tissue sectioning using full field optical coherence tomography

      According to a first aspect, the invention relates to a multimodal optical sectioning microscope (200, 400, 600) for full-field imaging of a volumic and scattering sample comprising: --a full-field OCT system for providing an image of a first section in depth of the sample comprising an illumination sub-system (201, 401, 601) and a full-field imaging interferometer with a detection sub system (208, 408, 608) and an optical conjugation device for optically conjugating the sample and said detection sub system, wherein said optical conjugation device comprises a microscope objective (203, 403, 603), --a supplementary full-field optical sectioning imaging system for providing ...

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    5. Optical coherence microscopy of living cells and bioengineered tissue dynamics in high-resolution cross-section

      Optical coherence microscopy of living cells and bioengineered tissue dynamics in high-resolution cross-section

      Optical coherence tomography (OCT) is a valuable tool in the cross-sectional observation/analysis of three-dimensional (3-D) biological tissues, and that histological observation is important clinically. However, the resolution of the technology is approximately 10–20 μm. In this study, optical coherence microscopy (OCM), a tomographic system combining OCT technology with a microscopic technique, was constructed for observing cells individually with a resolution at the submicrometer level. Cells and 3-D tissues fabricated by cell sheet technology were observed by OCM. Importantly, the cell nuclei and cytoplasm could be clearly distinguished, and the time-dependent dynamics of cell-sheet tissues could be observed in ...

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      Mentions: Panasonic
    6. Treatment monitoring of 0.5% 5-fluorouracil and 10% salicylic acid in clinical and subclinical actinic keratoses with the combination of optical coherence tomography and reflectance confocal microscopy

      Treatment monitoring of 0.5% 5-fluorouracil and 10% salicylic acid in clinical and subclinical actinic keratoses with the combination of optical coherence tomography and reflectance confocal microscopy

      Background Reflectance confocal microscopy (RCM) and high-definition optical coherence tomography (HD-OCT) allow the observation in vivo of dynamic changes in response to non-surgical treatment of actinic keratosis and field of cancerisation. Objective To non-invasively assess the pharmacodynamic changes induced by treatment with low dose 5-fluorouracil and 10% salicylic acid by means of RCM and high-definition OCT in field cancerization and actinic keratosis. Methods Twenty patients aged >50 years with diagnosis of actinic keratosis on the head and face and indication for treatment with 0.5% 5-fluorouracil and 10% salicylic acid were enrolled. An area of 25 cm² including visible type ...

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    7. Image contrast reduction mechanism in full-field optical coherence tomography

      Image contrast reduction mechanism in full-field optical coherence tomography

      Correct interpretation of image contrast obtained with full-field optical coherence tomography (FFOCT) technique is required for accurate medical diagnosis applications. In this work, first, the characteristics of microscopic structures of tissue that generate the contrast in en-face tomographic image obtained with FFOCT are discussed. Then an overview is given of the parameters that affect image contrast. Finally, the contrast correction factor for correct image interpretation and the contrast limits to practical FFOCT systems are outlined.

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      Mentions: Wanrong Gao
    8. Smart optical coherence tomography for ultra-deep imaging through highly scattering media

      Smart optical coherence tomography for ultra-deep imaging through highly scattering media

      Multiple scattering of waves in disordered media is a nightmare whether it be for detection or imaging purposes. The best approach so far to get rid of multiple scattering is optical coherence tomography. It basically combines confocal microscopy and coherence time-gating to discriminate ballistic photons from a predominant multiple scattering background. Nevertheless, the imaging depth range remains limited to 1 mm at best in human soft tissues. Here we propose a matrix approach of optical imaging to push back this fundamental limit. By combining a matrix discrimination of ballistic waves and iterative time-reversal, we show both theoretically and experimentally an ...

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    9. Fingerprint imaging from the inside of a finger with full-field optical coherence tomography

      Fingerprint imaging from the inside of a finger with full-field optical coherence tomography

      Imaging below fingertip surface might be a useful alternative to the traditional fingerprint sensing since the internal finger features are more reliable than the external ones. One of the most promising subsurface imaging technique is optical coherence tomography (OCT), which, however, has to acquire 3-D data even when a single en face image is required. This makes OCT inherently slow for en face imaging and produce unnecessary large data sets. Here we demonstrate that full-field optical coherence tomography (FF-OCT) can be used to produce en face images of sweat pores and internal fingerprints, which can be used for the identification ...

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    10. Autofocus System

      Autofocus System

      An autofocus apparatus is capable of detecting the position of a sample on a microscope. The sample may consist of a specimen mounted between a microscope slide and coverslip or specimens within a well plate. The device tracks the position of a sample by identifying refractive index boundaries through Fresnel reflections. A change in refractive index can correspond to the top and bottom of a coverslip, the top of a slide, the bottom of a well plate or the bottom of a well within a well plate. Using optical coherence tomography (OCT) these reflections are used to form a depth ...

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    11. Wide-field optical coherence microscopy of the mouse brain slice

      Wide-field optical coherence microscopy of the mouse brain slice

      The imaging capability of optical coherence microscopy (OCM) has great potential to be used in neuroscience research because it is able to visualize anatomic features of brain tissue without labeling or external contrast agents. However, the field of view of OCM is still narrow, which dilutes the strength of OCM and limits its application. In this study, we present fully automated wide-field OCM for mosaic imaging of sliced mouse brains. A total of 308 segmented OCM images were acquired, stitched, and reconstructed as an en-face brain image after intensive imaging processing. The overall imaging area was 11.2 × 7.0 ...

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    12. Wide-field, full-field optical coherence microscopy for high-axial-resolution phase and amplitude imaging

      Wide-field, full-field optical coherence microscopy for high-axial-resolution phase and amplitude imaging

      An original single-objective, full-field optical coherence microscopy system is reported that is capable of imaging both the phase and the amplitude of semi-transparent samples over a field of view of 17.5     mm × 17.5     mm with an axial sectioning resolution of 1.5 μm. A special stack acquisition arrangement ensures optimal reachable imaging depth. Several phase-shifting interferometry algorithms for phase measurement with broadband light are compared theoretically and experimentally. Using the phase information, noninvasive depth-resolved topographic images of multilayer samples are produced to characterize each layer by measuring their defects and curvature with a nanometric scale precision. Using the ...

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    13. Intrasurgical MICROSCOPE-INTEGRATED Spectral Domain Optical Coherence Tomography–assisted Membrane Peeling

      Intrasurgical MICROSCOPE-INTEGRATED Spectral Domain Optical Coherence Tomography–assisted Membrane Peeling

      Purpose: To evaluate microscope-integrated intrasurgical spectral domain optical coherence tomography during macular surgery in a prospective monocenter study. Methods: Before pars plana vitrectomy and before, during, and after membrane peeling, 512 × 128 macular cube scans were performed using a Carl Zeiss Meditec Cirrus high-definition OCT system adapted to the optical pathway of a Zeiss OPMI VISU 200 surgical microscope and compared with retinal staining. Results: The study included 51 patients with epiretinal membranes, with 8 of those having additional lamellar macular holes, 11 patients with vitreomacular traction, and 8 patients with full-thickness macular holes. Intraoperative spectral domain optical coherence tomography ...

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    14. A Circadian Clock Gene, Cry, Affects Heart Morphogenesis and Function in Drosophila as Revealed by Optical Coherence Microscopy

      A Circadian Clock Gene, Cry, Affects Heart Morphogenesis and Function in Drosophila as Revealed by Optical Coherence Microscopy

      Circadian rhythms are endogenous, entrainable oscillations of physical, mental and behavioural processes in response to local environmental cues such as daylight, which are present in the living beings, including humans. Circadian rhythms have been related to cardiovascular function and pathology. However, the role that circadian clock genes play in heart development and function in a whole animal in vivo are poorly understood. The Drosophila cryptochrome (dCry) is a circadian clock gene that encodes a major component of the circadian clock negative feedback loop. Compared to the embryonic stage, the relative expression levels of dCry showed a significant increase (>100-fold) in ...

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    15. Imaging accessory frame for integration with an operating microscope

      Imaging accessory frame for integration with an operating microscope

      Some embodiments of the present inventive concept provide an accessory frame including an optical coherence tomography (OCT) imaging head. The accessory frame includes a mounting plate including a microscope field lens attachment port and a microscope field plate anchor pin. The mounting plate is configured to receive an intermediate field lens in the microscope field lens attachment port such that the accessory frame shares the mounting plate in common with an intermediate field lens.

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    16. Microscope Integrated Intraoperative Spectral Domain Optical Coherence Tomography for Cataract Surgery: Uses and Applications

      Microscope Integrated Intraoperative Spectral Domain Optical Coherence Tomography for Cataract Surgery: Uses and Applications

      Aim : To demonstrate the uses and applications of a microscope integrated intraoperative Optical Coherence Tomography in Micro Incision Cataract Surgery (MICS) and Femtosecond Laser Assisted Cataract Surgery (FLACS). Methods : Intraoperative real time imaging using the RESCAN™ 700 (Carl Zeiss Meditec, Oberkochen, Germany) was done for patients undergoing MICS as well as FLACS. The OCT videos were reviewed at each step of the procedure and the findings were noted and analyzed. Results : Microscope Integrated Intraoperative Optical Coherence Tomography was found to be beneficial during all the critical steps of cataract surgery. We were able to qualitatively assess wound morphology in clear ...

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    17. Surgical microscopes using optical coherence tomography and related systems

      Surgical microscopes using optical coherence tomography and related systems

      Some embodiments of the present inventive concept provide optical coherence tomography (OCT) systems for integration with a microscope. The OCT system includes a sample arm coupled to the imaging path of a microscope. The sample arm includes an input beam zoom assembly including at least two movable lenses configured to provide shape control for an OCT signal beam; a scan assembly including at least one scanning mirror and configured for telecentric scanning of the OCT signal beam; and a beam expander configured to set the OCT signal beam diameter incident on the microscope objective. The shape control includes separable controls ...

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    18. Correlation between polarization sensitive optical coherence tomography and second harmonic generation microscopy in skin

      Correlation between polarization sensitive optical coherence tomography and second harmonic generation microscopy in skin

      Both polarization sensitive optical coherence tomography (PS-OCT) and second harmonic generation (SHG) microscopy are 3D optical imaging methods providing information related to collagen in the skin. PS-OCT provides birefringence information which is due to the collagen composition of the skin. SHG microscopy visualizes collagen fibers in the skin based on their SHG property. These two modalities have been applied to the same skin pathologies associated with collagen changes, but their relationship has not been examined. In this study, we tried to find the relationship by imaging the same skin samples with both modalities. Various parts of the normal rat skin ...

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      Mentions: Ki Hean Kim
    19. Characteristics of Pre-Descemet Membrane Corneal Dystrophy by Three Different Imaging Modalities—In Vivo Confocal Microscopy, Anterior Segment Optical Coherence Tomography, and Scheimpflug Corneal Densitometry Analysis

      Characteristics of Pre-Descemet Membrane Corneal Dystrophy by Three Different Imaging Modalities—In Vivo Confocal Microscopy, Anterior Segment Optical Coherence Tomography, and Scheimpflug Corneal Densitometry Analysis

      Purpose: To evaluate the characteristics of pre-Descemet membrane corneal dystrophy by 3 different imaging modalities: in vivo confocal microscopy (IVCM), anterior segment optical coherence tomography (ASOCT), and Scheimpflug corneal densitometry analysis. Methods: A 32-year-old male patient with pre-Descemet membrane corneal dystrophy was subjected to imaging by IVCM, ASOCT, and Scheimpflug tomography. Results: Slit-lamp biomicroscopy showed the presence of tiny pleomorphic opacities in the posterior stroma, immediately anterior to Descemet membrane bilaterally. On IVCM, pleomorphic, hyperreflective punctate particles were seen both intracellularly and extracellularly in the anterior and mid stroma with increased reflectivity of some keratocytes that, however, were of normal ...

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    20. Massachusetts General Hospital Receives NIH Grant for High Resolution Optical Imaging

      Massachusetts General Hospital Receives NIH Grant for High Resolution Optical Imaging

      Massachusetts General Hospital Receives a 2015 NIH Grant for $285,859 for High Resolution Optical Imaging. The principal investigator is David Boas. The program began in 2015 and ends in 2015. Below is a summary of the proposed work.

      This core will support the theme of functional coupling between glial, endothelial, and neuronal cells by using both in vivo and in vitro novel optical imaging to dissect the mechanisms of brain plasticity after cerebral ischemia . Novel optical imaging technologies have unique capability to measure the dynamics of glia, endothelium, and neurons as well as hemodynamic, metabolic, and electrophysiologic responses upon ...

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    21. Method and device for high resolution full field interference microscopy

      Method and device for high resolution full field interference microscopy

      The invention relates to an incoherent light full field interference microscopy device for the imaging of a volumetric scattering sample (106). The device comprises an interference device (100) between a reference wave (401), produced by reflection of an incident wave by a reflective surface (105) of a reference arm of the interference device, and an object wave (402) produced by backscattering of the incident wave by a slice of the sample, an acquisition device (108) for at least a first interference signal and at least a second interference signal resulting from the interference of the reference and object waves, the ...

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    22. Fibre-coupled multiphoton microscope with adaptive motion compensation

      Fibre-coupled multiphoton microscope with adaptive motion compensation

      To address the challenge of sample motion during in vivo imaging, we present a fibre-coupled multiphoton microscope with active axial motion compensation. The position of the sample surface is measured using optical coherence tomography and fed back to a piezo actuator that adjusts the axial location of the objective to compensate for sample motion. We characterise the system’s performance and demonstrate that it can compensate for axial sample velocities up to 700 µm/s. Finally we illustrate the impact of motion compensation when imaging multiphoton excited autofluorescence in ex vivo mouse skin.

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    23. Optical coherence photoacoustic microscopy for in vivo multimodal retinal imaging

      Optical coherence photoacoustic microscopy for in vivo multimodal retinal imaging

      We developed an optical coherence photoacoustic microscopy (OC-PAM) system, which can accomplish optical coherence tomography (OCT) and photoacoustic microscopy (PAM) simultaneously by using a single pulsed broadband light source. With a center wavelength of 800 nm and a bandwidth of 30 nm, the system is suitable for imaging the retina. Generated from the same group of photons, the OCT and PAM images are intrinsically registered in the lateral directions. To test the capabilities of the system on multimodal ophthalmic imaging, we imaged the retina of pigmented rats. The OCT images showed the retinal structures with quality similar to conventional OCT ...

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    24. Development of cellular resolution Gabor-domain optical coherence microscopy for biomedical applications

      Development of cellular resolution Gabor-domain optical coherence microscopy for biomedical applications

      We have developed a cellular resolution imaging modality, Gabor-Domain Optical Coherence Microscopy, which combines the high lateral resolution of confocal microscopy with the high sectioning capability of optical coherence tomography to image deep layers in tissues with high-contrast and volumetric resolution of 2 μm. A novelty of the custom microscope is the biomimetics that incorporates a liquid lens, as in whales’s eyes, for robust and rapid acquisition of volumetric imaging of deep layers in tissue down to 2 mm, thus overcoming the tradeoff between lateral resolution and depth of focus. The system incorporates a handheld scanning optical imaging head ...

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