1. Articles in category: Microscopy

    1-24 of 424 1 2 3 4 ... 16 17 18 »
    1. Your Upgrade Path to High Performance Intrasurgical OCT EnFocus™

      Your Upgrade Path to High Performance Intrasurgical OCT EnFocus™

      EnFocus is an intrasurgical OCT upgrade solution for new and existing surgical microscopes. See the details you are looking for with high-resolution, wide scan, and deep depth OCT images, providing subsurface visualization during surgery. Affordably add intrasurgical OCT directly into your surgical workflow for both anterior and posterior surgery. The EnFocus OCT is attached or removed within minutes; the modular design allows flexible sharing between multiple microscopes in your operating theater

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      Mentions: Bioptigen Leica
    2. Bimodal endoscopic probe combining white-light microscopy and optical coherence tomography

      Bimodal endoscopic probe combining white-light microscopy and optical coherence tomography

      We present a novel bimodal endoscopic imaging probe that can simultaneously provide full-field white-light video microscopy and confocal optical coherence tomography (OCT) depth scans. The two modalities rely on spectrally separated optical paths that run partially in parallel through a micro-optical bench system, which has a cross-section of only 2    mm × 2.76    mm and is realized via standard silicon micromachining techniques. With a numerical aperture of 0.061, the video modality has a resolution and field of view of 9.3 and 1240    μm × 1080    μm , respectively. The resolution is limited by the pixel spacing of the coherent fiber ...

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    3. Measuring refractive index and volume of liquid under high pressure with optical coherence tomography and light microscopy

      Measuring refractive index and volume of liquid under high pressure with optical coherence tomography and light microscopy

      Measuring the refractive index and volume of liquid under high pressure simultaneously is a big challenge. This paper proposed an alternative solution by combing optical coherence tomography with microscopy. An experiment for a feasibility study was carried out on polydimethylsiloxane liquid in a diamond anvil cell. The refractive index of the sample increased dramatically with pressure loaded, and the curve of pressure volume was also obtained.

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    4. EnFocus™ Surgical Microscope from Leica

      EnFocus™  Surgical Microscope from Leica

      EnFocus is an intrasurgical OCT upgrade solution for new and existing surgical microscopes. See the details you are looking for with high-resolution, wide scan, and deep depth OCT images, providing subsurface visualization during surgery. Affordably add intrasurgical OCT directly into your surgical workflow for both anterior and posterior surgery. The EnFocus OCT is attached or removed within minutes; the modular design allows flexible sharing between multiple microscopes in your operating theater.

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      Mentions: Leica
    5. GPU-based computational adaptive optics for volumetric optical coherence microscopy

      GPU-based computational adaptive optics for volumetric optical coherence microscopy

      Optical coherence tomography (OCT) is a non-invasive imaging technique that measures reflectance from within biological tissues. Current higher-NA optical coherence microscopy (OCM) technologies with near cellular resolution have limitations on volumetric imaging capabilities due to the trade-offs between resolution vs. depth-of-field and sensitivity to aberrations. Such trade-offs can be addressed using computational adaptive optics (CAO), which corrects aberration computationally for all depths based on the complex optical field measured by OCT. However, due to the large size of datasets plus the computational complexity of CAO and OCT algorithms, it is a challenge to achieve high-resolution 3D-OCM reconstructions at speeds suitable ...

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    6. Imaging of the stroke-related changes in the vascular system of the mouse brain with the use of extended focus Optical Coherence Microscopy

      Imaging of the stroke-related changes in the vascular system of the mouse brain with the use of extended focus Optical Coherence Microscopy

      We used Optical Coherence Microscopy (OCM) to monitor structural and functional changes due to ischemic stroke in small animals brains in vivo. To obtain lateral resolution of 2.2 μm over the range of 600 μm we used extended focus configuration of OCM instrument involving Bessel beam. It provided access to detailed 3D information about the changes in brain vascular system up to the level of capillaries across I and II/III layers of neocortex. We used photothrombotic stroke model involving photoactive application of rose bengal to assure minimal invasiveness of the procedure and precise localization of the clot distribution ...

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    7. Label-free imaging of developing vasculature in zebrafish with phase variance optical coherence microscopy

      Label-free imaging of developing vasculature in zebrafish with phase variance optical coherence microscopy

      A phase variance optical coherence microscope (pvOCM) has been created to visualize blood flow in the vasculature of zebrafish embryos, without using exogenous labels. The pvOCM imaging system has axial and lateral resolutions of 2 μm in tissue, and imaging depth of more than 100 μm. Imaging of 2–5 days post-fertilization zebrafish embryos identified the detailed structures of somites, spinal cord, gut and notochord based on intensity contrast. Visualization of the blood flow in the aorta, veins and intersegmental vessels was achieved with phase variance contrast. The pvOCM vasculature images were confirmed with corresponding fluorescence microscopy of a zebrafish ...

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    8. Automated interferometric synthetic aperture microscopy and computational adaptive optics for improved optical coherence tomography

      Automated interferometric synthetic aperture microscopy and computational adaptive optics for improved optical coherence tomography

      In this paper, we introduce an algorithm framework for the automation of interferometric synthetic aperture microscopy (ISAM). Under this framework, common processing steps such as dispersion correction, Fourier domain resampling, and computational adaptive optics aberration correction are carried out as metrics-assisted parameter search problems. We further present the results of this algorithm applied to phantom and biological tissue samples and compare with manually adjusted results. With the automated algorithm, near-optimal ISAM reconstruction can be achieved without manual adjustment. At the same time, the technical barrier for the nonexpert using ISAM imaging is also significantly lowered.

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    9. Spectral fusing Gabor domain optical coherence microscopy

      Spectral fusing Gabor domain optical coherence microscopy

      Gabor domain optical coherence microscopy (GD-OCM) is one of many variations of optical coherence tomography (OCT) techniques that aims for invariant high resolution across a 3D field of view by utilizing the ability to dynamically refocus the imaging optics in the sample arm. GD-OCM acquires multiple cross-sectional images at different focus positions of the objective lens, and then fuses them to obtain an invariant high-resolution 3D image of the sample, which comes with the intrinsic drawback of a longer processing time as compared to conventional Fourier domain OCT. Here, we report on an alternative Gabor fusing algorithm, the spectral-fusion technique ...

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    10. Classifying murine glomerulonephritis using optical coherence tomography and optical coherence elastography

      Classifying murine glomerulonephritis using optical coherence tomography and optical coherence elastography

      Elastic-wave propagation in mouse healthy and nephritic kidneys. Acute glomerulonephritis caused by antiglomerular basement membrane marked by high mortality. The primary reason for this is delayed diagnosis via blood examination, urine analysis, tissue biopsy, or ultrasound and X-ray computed tomography imaging. Blood, urine, and tissue-based diagnoses can be time consuming, while ultrasound and CT imaging have relatively low spatial resolution, with reduced sensitivity. Optical coherence tomography is a noninvasive and high-resolution imaging technique that provides superior spatial resolution (micrometer scale) as compared to ultrasound and CT. Changes in tissue properties can be detected based on the optical metrics analyzed from ...

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    11. Preliminary study of optical coherence tomography imaging to identify microscopic extrathyroidal extension in patients with papillary thyroid carcinoma

      Preliminary study of optical coherence tomography imaging to identify microscopic extrathyroidal extension in patients with papillary thyroid carcinoma

      Background and Objectives We evaluated the feasibility of using optical coherence tomography (OCT), to identify microscopic extrathyroidal extension (mETE) in ex vivo thyroidectomy specimens of patients who underwent thyroidectomy for the treatment of papillary thyroid carcinoma (PTC). Methods A total of 170 ex vivo OCT images of the tumor, were acquired just after completion of thyroidectomy in 17 patients. The OCT images of each patient were separately evaluated by two blinded investigators, and the outcomes were compared with the histopathology reports. Results The sensitivity and specificity of mETE identification from the OCT images were 81.4% and 86.0%, respectively ...

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    12. Full-field optical coherence microscopy with optimized ultrahigh spatial resolution

      Full-field optical coherence microscopy with optimized ultrahigh spatial resolution

      Full-field optical coherence microscopy (FF-OCM) with isotropic spatial resolution of 0.5 μm (in water), at 700 nm center wavelength, is reported. A theoretical study of the FF-OCM axial response is carried out for maximizing the axial resolution of the system, considering the effect of optical dispersion. The lateral resolution is optimized by using water-immersion microscope objectives with a numerical aperture of 1.2. This ultrahigh-resolution FF-OCM system is applied to animal and human skin tissue imaging, revealing ultra-fine in-depth structures at the sub-cellular level.

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      Mentions: Arnaud Dubois
    13. Optical tissue sectioning using full field optical coherence tomography

      Optical tissue sectioning using full field optical coherence tomography

      According to a first aspect, the invention relates to a multimodal optical sectioning microscope (200, 400, 600) for full-field imaging of a volumic and scattering sample comprising: --a full-field OCT system for providing an image of a first section in depth of the sample comprising an illumination sub-system (201, 401, 601) and a full-field imaging interferometer with a detection sub system (208, 408, 608) and an optical conjugation device for optically conjugating the sample and said detection sub system, wherein said optical conjugation device comprises a microscope objective (203, 403, 603), --a supplementary full-field optical sectioning imaging system for providing ...

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    14. Optical coherence microscopy of living cells and bioengineered tissue dynamics in high-resolution cross-section

      Optical coherence microscopy of living cells and bioengineered tissue dynamics in high-resolution cross-section

      Optical coherence tomography (OCT) is a valuable tool in the cross-sectional observation/analysis of three-dimensional (3-D) biological tissues, and that histological observation is important clinically. However, the resolution of the technology is approximately 10–20 μm. In this study, optical coherence microscopy (OCM), a tomographic system combining OCT technology with a microscopic technique, was constructed for observing cells individually with a resolution at the submicrometer level. Cells and 3-D tissues fabricated by cell sheet technology were observed by OCM. Importantly, the cell nuclei and cytoplasm could be clearly distinguished, and the time-dependent dynamics of cell-sheet tissues could be observed in ...

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      Mentions: Panasonic
    15. Treatment monitoring of 0.5% 5-fluorouracil and 10% salicylic acid in clinical and subclinical actinic keratoses with the combination of optical coherence tomography and reflectance confocal microscopy

      Treatment monitoring of 0.5% 5-fluorouracil and 10% salicylic acid in clinical and subclinical actinic keratoses with the combination of optical coherence tomography and reflectance confocal microscopy

      Background Reflectance confocal microscopy (RCM) and high-definition optical coherence tomography (HD-OCT) allow the observation in vivo of dynamic changes in response to non-surgical treatment of actinic keratosis and field of cancerisation. Objective To non-invasively assess the pharmacodynamic changes induced by treatment with low dose 5-fluorouracil and 10% salicylic acid by means of RCM and high-definition OCT in field cancerization and actinic keratosis. Methods Twenty patients aged >50 years with diagnosis of actinic keratosis on the head and face and indication for treatment with 0.5% 5-fluorouracil and 10% salicylic acid were enrolled. An area of 25 cm² including visible type ...

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    16. Image contrast reduction mechanism in full-field optical coherence tomography

      Image contrast reduction mechanism in full-field optical coherence tomography

      Correct interpretation of image contrast obtained with full-field optical coherence tomography (FFOCT) technique is required for accurate medical diagnosis applications. In this work, first, the characteristics of microscopic structures of tissue that generate the contrast in en-face tomographic image obtained with FFOCT are discussed. Then an overview is given of the parameters that affect image contrast. Finally, the contrast correction factor for correct image interpretation and the contrast limits to practical FFOCT systems are outlined.

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      Mentions: Wanrong Gao
    17. Smart optical coherence tomography for ultra-deep imaging through highly scattering media

      Smart optical coherence tomography for ultra-deep imaging through highly scattering media

      Multiple scattering of waves in disordered media is a nightmare whether it be for detection or imaging purposes. The best approach so far to get rid of multiple scattering is optical coherence tomography. It basically combines confocal microscopy and coherence time-gating to discriminate ballistic photons from a predominant multiple scattering background. Nevertheless, the imaging depth range remains limited to 1 mm at best in human soft tissues. Here we propose a matrix approach of optical imaging to push back this fundamental limit. By combining a matrix discrimination of ballistic waves and iterative time-reversal, we show both theoretically and experimentally an ...

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    18. Fingerprint imaging from the inside of a finger with full-field optical coherence tomography

      Fingerprint imaging from the inside of a finger with full-field optical coherence tomography

      Imaging below fingertip surface might be a useful alternative to the traditional fingerprint sensing since the internal finger features are more reliable than the external ones. One of the most promising subsurface imaging technique is optical coherence tomography (OCT), which, however, has to acquire 3-D data even when a single en face image is required. This makes OCT inherently slow for en face imaging and produce unnecessary large data sets. Here we demonstrate that full-field optical coherence tomography (FF-OCT) can be used to produce en face images of sweat pores and internal fingerprints, which can be used for the identification ...

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    19. Autofocus System

      Autofocus System

      An autofocus apparatus is capable of detecting the position of a sample on a microscope. The sample may consist of a specimen mounted between a microscope slide and coverslip or specimens within a well plate. The device tracks the position of a sample by identifying refractive index boundaries through Fresnel reflections. A change in refractive index can correspond to the top and bottom of a coverslip, the top of a slide, the bottom of a well plate or the bottom of a well within a well plate. Using optical coherence tomography (OCT) these reflections are used to form a depth ...

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    20. Wide-field optical coherence microscopy of the mouse brain slice

      Wide-field optical coherence microscopy of the mouse brain slice

      The imaging capability of optical coherence microscopy (OCM) has great potential to be used in neuroscience research because it is able to visualize anatomic features of brain tissue without labeling or external contrast agents. However, the field of view of OCM is still narrow, which dilutes the strength of OCM and limits its application. In this study, we present fully automated wide-field OCM for mosaic imaging of sliced mouse brains. A total of 308 segmented OCM images were acquired, stitched, and reconstructed as an en-face brain image after intensive imaging processing. The overall imaging area was 11.2 × 7.0 ...

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    21. Wide-field, full-field optical coherence microscopy for high-axial-resolution phase and amplitude imaging

      Wide-field, full-field optical coherence microscopy for high-axial-resolution phase and amplitude imaging

      An original single-objective, full-field optical coherence microscopy system is reported that is capable of imaging both the phase and the amplitude of semi-transparent samples over a field of view of 17.5     mm × 17.5     mm with an axial sectioning resolution of 1.5 μm. A special stack acquisition arrangement ensures optimal reachable imaging depth. Several phase-shifting interferometry algorithms for phase measurement with broadband light are compared theoretically and experimentally. Using the phase information, noninvasive depth-resolved topographic images of multilayer samples are produced to characterize each layer by measuring their defects and curvature with a nanometric scale precision. Using the ...

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    22. Intrasurgical MICROSCOPE-INTEGRATED Spectral Domain Optical Coherence Tomography–assisted Membrane Peeling

      Intrasurgical MICROSCOPE-INTEGRATED Spectral Domain Optical Coherence Tomography–assisted Membrane Peeling

      Purpose: To evaluate microscope-integrated intrasurgical spectral domain optical coherence tomography during macular surgery in a prospective monocenter study. Methods: Before pars plana vitrectomy and before, during, and after membrane peeling, 512 × 128 macular cube scans were performed using a Carl Zeiss Meditec Cirrus high-definition OCT system adapted to the optical pathway of a Zeiss OPMI VISU 200 surgical microscope and compared with retinal staining. Results: The study included 51 patients with epiretinal membranes, with 8 of those having additional lamellar macular holes, 11 patients with vitreomacular traction, and 8 patients with full-thickness macular holes. Intraoperative spectral domain optical coherence tomography ...

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    23. A Circadian Clock Gene, Cry, Affects Heart Morphogenesis and Function in Drosophila as Revealed by Optical Coherence Microscopy

      A Circadian Clock Gene, Cry, Affects Heart Morphogenesis and Function in Drosophila as Revealed by Optical Coherence Microscopy

      Circadian rhythms are endogenous, entrainable oscillations of physical, mental and behavioural processes in response to local environmental cues such as daylight, which are present in the living beings, including humans. Circadian rhythms have been related to cardiovascular function and pathology. However, the role that circadian clock genes play in heart development and function in a whole animal in vivo are poorly understood. The Drosophila cryptochrome (dCry) is a circadian clock gene that encodes a major component of the circadian clock negative feedback loop. Compared to the embryonic stage, the relative expression levels of dCry showed a significant increase (>100-fold) in ...

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    1-24 of 424 1 2 3 4 ... 16 17 18 »
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