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(14 articles) Harvard University
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(10 articles) University of Illinois at Urbana-Champaign
(8 articles) University of Kent
(6 articles) Washington University in St. Louis
(5 articles) University of Central Florida
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System and method for enhancing microscopic images of tissue
Explore Article PatFT » Page 1 of 1 (Mar 9 2010) Microscopy , Patents
A system and method for enhancing images of ex-vivo or in-vivo tissue produced by confocal microscopy, optical coherence tomography, two-photon microscopy, or ultrasound, is provided by applying to the tissue a solution or gel having an effective concentration of citric or other alpha-hydroxy acid which enhances tissue structures, such as cellular nuclei, in such images. Such concentration may be 3-20% acid, and preferably 5% acid. -
In vivo, dual-modality imaging of mouse eyes: optical coherence tomography and photoacoustic microscopy within a single instrument
Explore Article SPIE Digital Library (Mar 2 2010) Microscopy , Ophthalmology
Optical-resolution photoacoustic microscopy (OR-PAM) provides superb optical absorption contrast for red blood cells (RBCs), which makes it ideal for in vivo microvasculature imaging. In comparison, optical coherence tomography (OCT), widely used for tissue microstructure imaging, provides high optical scattering contrast. The two contrast mechanisms are highly complementary. In this work, we combined OR-PAM and OCT into a single, dual-modality imaging instrument for in vivo mouse eye imaging. We demonstrated in vivo dual-modality imaging of the anterior segment of mouse eyes with laser pulse energy within the ANSI laser safety standard.
Comment on Article Mentions: Li Li Lihong V. Wang Konstantin I. Maslov
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Spectrally encoded confocal scanning laser ophthalmoscope
Explore Article SPIE Digital Library (Mar 2 2010) Microscopy , Ophthalmology
Fundus imaging has become an essential clinical diagnostic tool in ophthalmology. Current generation scanning laser ophthalmoscopes (SLO) offer advantages over conventional fundus photography and indirect ophthalmoscopy in terms of light efficiency and contrast. As a result of the ability of SLO to provide rapid, continuous imaging of retinal structures and its versatility in accommodating a variety of illumination wavelengths, allowing for imaging of both endogenous and exogenous fluorescent contrast agents, SLO has become a powerful tool for the characterization of retinal pathologies. However, common implementations of SLO, such as the confocal scanning laser ophthalmoscope (CSLO) and line-scanning laser ophthalmoscope (LSLO), ...
Comment on Article Mentions: Joseph A. Izatt Duke University
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Cellular resolution ex vivo imaging of gastrointestinal tissues with optical coherence microscopy
Explore Article SPIE Digital Library (Mar 2 2010) Microscopy
Optical coherence microscopy (OCM) combines confocal microscopy and optical coherence tomography (OCT) to improve imaging depth and contrast, enabling cellular imaging in human tissues. We aim to investigate OCM for ex vivo imaging of upper and lower gastrointestinal tract tissues, to establish correlations between OCM imaging and histology, and to provide a baseline for future endoscopic studies. Co-registered OCM and OCT imaging were performed on fresh surgical specimens and endoscopic biopsy specimens, and images were correlated with histology. Imaging was performed at 1.06-µm wavelength with <2-µm transverse and <4-µm axial resolution for OCM, and at 14-µm transverse and <3-µm axial ...
Comment on Article Mentions: Yu Chen Aaron D. Aguirre James G. Fujimoto
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Integrated optical coherence tomography and optical coherence microscopy imaging of human pathology
Explore Article SPIE Digital Library (Feb 24 2010) Microscopy
Excisional biopsy is the current gold standard for disease diagnosis; however, it requires a relatively long processing time and it may also suffer from unacceptable false negative rates due to sampling errors. Optical coherence tomography (OCT) is a promising imaging technique that provide real-time, high resolution and three-dimensional (3D) images of tissue morphology. Optical coherence microscopy (OCM) is an extension of OCT, combining both the coherence gating and the confocal gating techniques. OCM imaging achieves cellular resolution with deeper imaging depth compared to confocal microscopy. An integrated OCT/OCM imaging system can provide co-registered multiscale imaging of tissue morphology. 3D-OCT provides ...
Comment on Article Mentions: Massachusetts Institute of Technology James G. Fujimoto Aaron D. Aguirre
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A dual modality fluorescence confocal and optical coherence tomography microendoscope
Explore Article SPIE Digital Library (Feb 24 2010) Endoscopy , Microscopy , Oncology
We demonstrate the implementation of a Fourier domain optical coherence tomography (OCT) imaging system incorporated into the optical train of a fluorescence confocal microendoscope. The slit-scanning confocal system has been presented previously and achieves 3µm lateral resolution and 25µm axial resolution over a field of view of 430µm. Its multi-spectral mode of operation captures images with 6nm average spectral resolution. To incorporate OCT imaging, a common-path interferometer is made with a super luminescent diode and a reference coverslip located at the distal end of the fiber bundle catheter. The infrared diode spectral width allows a theoretical OCT axial resolution of ...
Comment on Article Mentions: University of Arizona
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Fast-scanning reflection-mode integrated optical-coherence and photoacoustic microscopy
Explore Article SPIE Digital Library (Feb 23 2010) Microscopy
We previously demonstrated that multimodal microscopy combining photoacoustic microscopy and optical coherence tomography can provide comprehensive insight into biological tissue at µm-level resolution by exploiting both optical absorption and scattering contrasts. Recently, we have developed a second-generation integrated photoacoustic and optical-coherence microscope, which can potentially be adapted for clinical applications. In this new system, we can perform photoacoustic and optical-coherence imaging simultaneously at a speed of 5,000 A-lines per second with real-time on-screen display. Also, both modalities now work in reflection mode instead of transmission mode, allowing easy access to various anatomical locations of interest. Imaging of skin and eye ...
Comment on Article Mentions: Li Li Lihong V. Wang Konstantin I. Maslov
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Ex vivo imaging of human thyroid pathology using integrated optical coherence tomography and optical coherence microscopy
Explore Article SPIE Digital Library (Feb 22 2010) Microscopy
We evaluate the feasibility of optical coherence tomography (OCT) and optical coherence microscopy (OCM) for imaging of benign and malignant thyroid lesions ex vivo using intrinsic optical contrast. 34 thyroid gland specimens are imaged from 17 patients, covering a spectrum of pathology ranging from normal thyroid to benign disease/neoplasms (multinodular colloid goiter, Hashimoto's thyroiditis, and follicular adenoma) and malignant thyroid tumors (papillary carcinoma and medullary carcinoma). Imaging is performed using an integrated OCT and OCM system, with <4 µm axial resolution (OCT and OCM), and 14 µm (OCT) and <2 µm (OCM) transverse resolution. The system allows seamless switching between ...
Comment on Article Mentions: Massachusetts Institute of Technology Aaron D. Aguirre Harvard University
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Focusing light through living tissue
Explore Article SPIE Digital Library (Feb 19 2010) Developmental Biology , Microscopy
Tissues such as skin, fat or cuticle are non-transparent because inhomogeneities in the tissue scatter light. We demonstrate experimentally that light can be focused through turbid layers of living tissue, in spite of scattering. Our method is based on the fact that coherent light forms an interference pattern, even after hundreds of scattering events. By spatially shaping the wavefront of the incident laser beam, this interference pattern was modified to make the scattered light converge to a focus. In contrast to earlier experiments, where light was focused through solid objects, we focused light through living pupae of Drosophila melanogaster. We ...
Comment on Article Mentions: University of Zurich
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High speed optical coherence microscopy with autofocus adjustment and a miniaturized endoscopic imaging probe
Explore Article opticsinfobase.org (Feb 17 2010) Endoscopy , Microscopy , Probes
ical coherence microscopy (OCM) is a promising technique for high resolution cellular imaging in human tissues. An OCM system for high-speed en face cellular resolution imaging was developed at 1060 nm wavelength at frame rates up to 5 Hz with resolutions of < 4 µm axial and < 2 µm transverse. The system utilized a novel polarization compensation method to combat wavelength dependent source polarization and achieve broadband electro-optic phase modulation compatible with ultrahigh axial resolution. In addition, the system incorporated an auto-focusing feature that enables precise, near real-time alignment of the confocal and coherence gates in tissue, allowing user-friendly ...
Comment on Article Mentions: James G. Fujimoto Shu-Wei Huang Aaron D. Aguirre
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Investigation of pore structure and cell distribution in EH-PEG hydrogel scaffold using optical coherence tomography and fluorescence microscopy
Explore Article SPIE Digital Library (Feb 11 2010) Microscopy
Macroporous EH-PEG hydrogels fabricated by porogen-leaching method are characterized by optical coherence tomography (OCT). High-resolution OCT visualizes the microstructures of the engineered tissue scaffolds in threedimensions. It also enables subsequent image processing to investigate several key morphological design parameters for macroporous scaffolds. Image processing algorithms are then presented to automatically quantify the pore size, porosity, and pore interconnectivity. The results indicated that those parameters highly depend on the porogen size. Further, fluorescence imaging was conducted to monitor the population of labeled human mesenchymal stem cells (hMSCs) loaded on the surface of the scaffolds. The results revealed the hMSCs' viability as ...
Comment on Article Mentions: Thorlabs Alex E. Cable Yu Chen
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Colorimetric three-dimensional microscopy
Explore Article PatFT » Page 1 of 1 (Feb 9 2010) Microscopy , Patents
An optically reflective or translucent object (14) can be microscopically imaged in all three dimensions and in true color for observation by a human observer. An interferometric optical setup is employed, using the low temporal coherence of a tunable broad-band light source (10, 20) to resolve the axial dimension, a single opto-mechanical or electronic scanning mechanism for accessing different object depths, and a two-dimensional photo sensor device (15, 34) capable of demodulating the temporally or spatially modulated scanning signals to reconstruct the object's full volume. Three volume scans are carried out sequentially, and the tunable broad-band source (10, 20) is ...
Comment on Article Mentions: Heliotis AG
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Gabor-based fusion technique for Optical Coherence Microscopy
Explore Article opticsinfobase.org (Feb 4 2010) Dermatology , Developmental Biology , Microscopy
We recently reported on an Optical Coherence Microscopy technique, whose innovation intrinsically builds on a recently reported - 2 μm invariant lateral resolution by design throughout a 2 mm cubic full-field of view - liquid-lens-based dynamic focusing optical probe [Murali et al.,Optics Letters 34, 145-147, 2009]. We shall report in this paper on the image acquisition enabled by this optical probe when combined with an automatic data fusion method developed and described here to produce an in-focus high resolution image throughout the imaging depth of the sample. An African frog tadpole (Xenopus laevis) was imaged with the novel probe and ...
Comment on Article Mentions: University of Rochester University of Central Florida Jannick P. Rolland
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Photoacoustic imaging and characterization of the microvasculature
Explore Article SPIE Digital Library (Jan 21 2010) Microscopy
Photoacoustic (optoacoustic) tomography, combining optical absorption contrast and highly scalable spatial resolution (from micrometer optical resolution to millimeter acoustic resolution), has broken through the fundamental penetration limit of optical ballistic imaging modalities—including confocal microscopy, two-photon microscopy, and optical coherence tomography—and has achieved high spatial resolution at depths down to the diffusive regime. Optical absorption contrast is highly desirable for microvascular imaging and characterization because of the presence of endogenous strongly light-absorbing hemoglobin. We focus on the current state of microvascular imaging and characterization based on photoacoustics. We first review the three major embodiments of photoacoustic tomography: microscopy, computed tomography, and ...
Comment on Article Mentions: Lihong V. Wang Washington University in St. Louis
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Partially coherent illumination for inverse scattering full-field interferometric synthetic aperture microscopy
Explore Article PatFT » Page 1 of 1 (Jan 4 2010) Microscopy , Patents
Methods and apparatus for three-dimensional imaging of a sample. A source is provided of a beam of light characterized by partial spatial coherence. The beam is focused onto a sample and scattered light from the sample is superposed with a reference beam derived from the source onto a focal plane detector array to provide an interference signal. A forward scattering model is derived relating measurement data to structure of an object to allow solutions of an inverse scattering problem, based upon the interference signal so that a three-dimensional structure of the same may be inferred. The partial spatial coherence of ...Comment on Article Mentions: Stephen A. Boppart University of Illinois at Urbana-Champaign Daniel L. Marks
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Eric Swanson » Jim Fujimoto video: Biophotonics and optical coherence tomography
Great video Jim!
dariemihaela » Gary S. Mintz
Dear Prof. Dr. Gary S. Mintz, You are an extraordinary expert in imaging, an intelligence ...
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