1. Articles in category: Microscopy

    1-24 of 431 1 2 3 4 ... 16 17 18 »
    1. Method and apparatus for automated scanning probe microscopy

      Method and apparatus for automated scanning probe microscopy

      This invention concerns scanning probe microscopes and related instruments ("SPMs") when used to investigate or measure large samples whose size is a multiple of the typical operational scanning area of an SPM, say 150 .mu.m.times.150 .mu.m at most. To avoid frequent readjustments or other time-consuming human interaction and errors when focusing the SPM, a multi-step, automated method for the SPM-scanning of large samples is disclosed, comprising a "coarse", i.e. low resolution, non-SPM scanning or mapping step adapted to scan a large sample and providing an integral map of the sample, followed by a preferably mathematical ...

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    2. Optical measurement apparatus and optical measurement method

      Optical measurement apparatus and optical measurement method

      A small and inexpensive optical measurement apparatus is provided in which noise due to optical interference such as inter-layer crosstalk or speckle is suppressed. The optical measurement apparatus includes a light source to emit laser light, a high-frequency superimposing unit to superimpose a high-frequency current on a driving current to drive the light source, a light branching element to branch the laser light into signal light and reference light, an objective lens to condense and irradiate the signal light on a measurement object, a condensing position scanning unit to scan a condensing position of the signal light, a light path ...

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    3. Optical coherence photoacoustic microscopy

      Optical coherence photoacoustic microscopy

      A system and method for providing an optical coherence photoacoustic (OC-PAM) microscopy. An OC-PAM microscope includes a light source that outputs light, a scanner, a detector, a transducer, and an image processing module. The scanner receives the light and scans the light across a sample. The detector receives reflected light from the sample in response to the scanned light. The transducer detects photoacoustic waves induced in the sample by the scanned light. The image processing module receives output from the detector and the transducer and generates a photoacoustic microscopy (PAM) image and an optical coherence tomography (OCT) image based on ...

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    4. Optical Coherence Tomography for Skin Cancer

      Optical Coherence Tomography for Skin Cancer

      Skin cancer is the most common malignancy worldwide, and non-melanoma skin cancers (NMSC) in particular, including basal cell carcinomas (BCC) and squamous cell carcinomas, constitute around 80 percent of all skin cancers. It is estimated that approximately 3.5 million new cases of NMSC occur every year in the U.S., exceeding that of all other cancers combined. Despite the high prevalence of NMSC, they can be effectively treated and cured with low recurrence when detected at an early stage. BCCs are known to be slow growing and rarely metastasize to other parts of the body; however, if left untreated ...

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    5. Dermoscopy, confocal microscopy and optical coherence tomography for the diagnosis of bedbug infestation

      Dermoscopy, confocal microscopy and optical coherence tomography for the diagnosis of bedbug infestation

      Letter to the editor: A 33 year-old woman suffering from rosacea presented with outbreaks of itchy plaques on her face and limbs that curiously improved when she was far from home during summer holidays (Fig. 1). Dermoscopy (FotoFinder Systems GmbH, Bad Birnbach, Germany) showed petechiae (Fig. 2A) and reflectance confocal microscopy (RCM; Vivascope 3000 ® , Caliber, New York, USA, distributed in Europe by MAVIG GmbH, München, Germany) showed intraepidermal vesicles (Fig. 2B) suggesting parasite bites. RCM found Demodex folliculorum (Fig. 2C) that could have been responsible for rosacea. However, a careful examination with dermoscopy identified a brown oval-shaped wingless insect ...

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      Mentions: AGFA Healthcare
    6. Velocity gradients in spatially resolved laser Doppler flowmetry and dynamic light scattering with confocal and coherence gating

      Velocity gradients in spatially resolved laser Doppler flowmetry and dynamic light scattering with confocal and coherence gating

      Dynamic light scattering (DLS) is widely used to characterize diffusive motion to obtain precise information on colloidal suspensions by calculating the autocorrelation function of the signal from a heterodyne optical system. DLS can also be used to determine the flow velocity field in systems that exhibit mass transport by incorporating the effects of the deterministic motion of scatterers on the autocorrelation function, a technique commonly known as laser Doppler flowmetry. DLS measurements can be localized with confocal and coherence gating techniques such as confocal microscopy and optical coherence tomography, thereby enabling the determination of the spatially resolved velocity field in ...

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    7. Heads up displays for optical coherence tomography integrated surgical microscopes

      Heads up displays for optical coherence tomography integrated surgical microscopes

      Surgical microscope systems are provided including an optical coherence tomography (OCT) system; an objective lens; oculars for direct viewing of a subject distal to the objective lens; a heads up display module configured to direct an optical image through the oculars to be visible to a user of at least one ocular; and a coupling element connected to the surgical microscope coupling the OCT system, the heads up display module and the objective lens. The coupling element has first and second faces, the first face positioned toward the oculars of the surgical microscope and the second face positioned toward the ...

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    8. Your Upgrade Path to High Performance Intrasurgical OCT EnFocus™

      Your Upgrade Path to High Performance Intrasurgical OCT EnFocus™

      EnFocus is an intrasurgical OCT upgrade solution for new and existing surgical microscopes. See the details you are looking for with high-resolution, wide scan, and deep depth OCT images, providing subsurface visualization during surgery. Affordably add intrasurgical OCT directly into your surgical workflow for both anterior and posterior surgery. The EnFocus OCT is attached or removed within minutes; the modular design allows flexible sharing between multiple microscopes in your operating theater

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      Mentions: Bioptigen Leica
    9. Bimodal endoscopic probe combining white-light microscopy and optical coherence tomography

      Bimodal endoscopic probe combining white-light microscopy and optical coherence tomography

      We present a novel bimodal endoscopic imaging probe that can simultaneously provide full-field white-light video microscopy and confocal optical coherence tomography (OCT) depth scans. The two modalities rely on spectrally separated optical paths that run partially in parallel through a micro-optical bench system, which has a cross-section of only 2    mm × 2.76    mm and is realized via standard silicon micromachining techniques. With a numerical aperture of 0.061, the video modality has a resolution and field of view of 9.3 and 1240    μm × 1080    μm , respectively. The resolution is limited by the pixel spacing of the coherent fiber ...

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    10. Measuring refractive index and volume of liquid under high pressure with optical coherence tomography and light microscopy

      Measuring refractive index and volume of liquid under high pressure with optical coherence tomography and light microscopy

      Measuring the refractive index and volume of liquid under high pressure simultaneously is a big challenge. This paper proposed an alternative solution by combing optical coherence tomography with microscopy. An experiment for a feasibility study was carried out on polydimethylsiloxane liquid in a diamond anvil cell. The refractive index of the sample increased dramatically with pressure loaded, and the curve of pressure volume was also obtained.

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    11. EnFocus™ Surgical Microscope from Leica

      EnFocus™  Surgical Microscope from Leica

      EnFocus is an intrasurgical OCT upgrade solution for new and existing surgical microscopes. See the details you are looking for with high-resolution, wide scan, and deep depth OCT images, providing subsurface visualization during surgery. Affordably add intrasurgical OCT directly into your surgical workflow for both anterior and posterior surgery. The EnFocus OCT is attached or removed within minutes; the modular design allows flexible sharing between multiple microscopes in your operating theater.

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      Mentions: Leica
    12. GPU-based computational adaptive optics for volumetric optical coherence microscopy

      GPU-based computational adaptive optics for volumetric optical coherence microscopy

      Optical coherence tomography (OCT) is a non-invasive imaging technique that measures reflectance from within biological tissues. Current higher-NA optical coherence microscopy (OCM) technologies with near cellular resolution have limitations on volumetric imaging capabilities due to the trade-offs between resolution vs. depth-of-field and sensitivity to aberrations. Such trade-offs can be addressed using computational adaptive optics (CAO), which corrects aberration computationally for all depths based on the complex optical field measured by OCT. However, due to the large size of datasets plus the computational complexity of CAO and OCT algorithms, it is a challenge to achieve high-resolution 3D-OCM reconstructions at speeds suitable ...

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    13. Imaging of the stroke-related changes in the vascular system of the mouse brain with the use of extended focus Optical Coherence Microscopy

      Imaging of the stroke-related changes in the vascular system of the mouse brain with the use of extended focus Optical Coherence Microscopy

      We used Optical Coherence Microscopy (OCM) to monitor structural and functional changes due to ischemic stroke in small animals brains in vivo. To obtain lateral resolution of 2.2 μm over the range of 600 μm we used extended focus configuration of OCM instrument involving Bessel beam. It provided access to detailed 3D information about the changes in brain vascular system up to the level of capillaries across I and II/III layers of neocortex. We used photothrombotic stroke model involving photoactive application of rose bengal to assure minimal invasiveness of the procedure and precise localization of the clot distribution ...

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    14. Label-free imaging of developing vasculature in zebrafish with phase variance optical coherence microscopy

      Label-free imaging of developing vasculature in zebrafish with phase variance optical coherence microscopy

      A phase variance optical coherence microscope (pvOCM) has been created to visualize blood flow in the vasculature of zebrafish embryos, without using exogenous labels. The pvOCM imaging system has axial and lateral resolutions of 2 μm in tissue, and imaging depth of more than 100 μm. Imaging of 2–5 days post-fertilization zebrafish embryos identified the detailed structures of somites, spinal cord, gut and notochord based on intensity contrast. Visualization of the blood flow in the aorta, veins and intersegmental vessels was achieved with phase variance contrast. The pvOCM vasculature images were confirmed with corresponding fluorescence microscopy of a zebrafish ...

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    15. Automated interferometric synthetic aperture microscopy and computational adaptive optics for improved optical coherence tomography

      Automated interferometric synthetic aperture microscopy and computational adaptive optics for improved optical coherence tomography

      In this paper, we introduce an algorithm framework for the automation of interferometric synthetic aperture microscopy (ISAM). Under this framework, common processing steps such as dispersion correction, Fourier domain resampling, and computational adaptive optics aberration correction are carried out as metrics-assisted parameter search problems. We further present the results of this algorithm applied to phantom and biological tissue samples and compare with manually adjusted results. With the automated algorithm, near-optimal ISAM reconstruction can be achieved without manual adjustment. At the same time, the technical barrier for the nonexpert using ISAM imaging is also significantly lowered.

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    16. Spectral fusing Gabor domain optical coherence microscopy

      Spectral fusing Gabor domain optical coherence microscopy

      Gabor domain optical coherence microscopy (GD-OCM) is one of many variations of optical coherence tomography (OCT) techniques that aims for invariant high resolution across a 3D field of view by utilizing the ability to dynamically refocus the imaging optics in the sample arm. GD-OCM acquires multiple cross-sectional images at different focus positions of the objective lens, and then fuses them to obtain an invariant high-resolution 3D image of the sample, which comes with the intrinsic drawback of a longer processing time as compared to conventional Fourier domain OCT. Here, we report on an alternative Gabor fusing algorithm, the spectral-fusion technique ...

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    17. Classifying murine glomerulonephritis using optical coherence tomography and optical coherence elastography

      Classifying murine glomerulonephritis using optical coherence tomography and optical coherence elastography

      Elastic-wave propagation in mouse healthy and nephritic kidneys. Acute glomerulonephritis caused by antiglomerular basement membrane marked by high mortality. The primary reason for this is delayed diagnosis via blood examination, urine analysis, tissue biopsy, or ultrasound and X-ray computed tomography imaging. Blood, urine, and tissue-based diagnoses can be time consuming, while ultrasound and CT imaging have relatively low spatial resolution, with reduced sensitivity. Optical coherence tomography is a noninvasive and high-resolution imaging technique that provides superior spatial resolution (micrometer scale) as compared to ultrasound and CT. Changes in tissue properties can be detected based on the optical metrics analyzed from ...

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    18. Preliminary study of optical coherence tomography imaging to identify microscopic extrathyroidal extension in patients with papillary thyroid carcinoma

      Preliminary study of optical coherence tomography imaging to identify microscopic extrathyroidal extension in patients with papillary thyroid carcinoma

      Background and Objectives We evaluated the feasibility of using optical coherence tomography (OCT), to identify microscopic extrathyroidal extension (mETE) in ex vivo thyroidectomy specimens of patients who underwent thyroidectomy for the treatment of papillary thyroid carcinoma (PTC). Methods A total of 170 ex vivo OCT images of the tumor, were acquired just after completion of thyroidectomy in 17 patients. The OCT images of each patient were separately evaluated by two blinded investigators, and the outcomes were compared with the histopathology reports. Results The sensitivity and specificity of mETE identification from the OCT images were 81.4% and 86.0%, respectively ...

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    19. Full-field optical coherence microscopy with optimized ultrahigh spatial resolution

      Full-field optical coherence microscopy with optimized ultrahigh spatial resolution

      Full-field optical coherence microscopy (FF-OCM) with isotropic spatial resolution of 0.5 μm (in water), at 700 nm center wavelength, is reported. A theoretical study of the FF-OCM axial response is carried out for maximizing the axial resolution of the system, considering the effect of optical dispersion. The lateral resolution is optimized by using water-immersion microscope objectives with a numerical aperture of 1.2. This ultrahigh-resolution FF-OCM system is applied to animal and human skin tissue imaging, revealing ultra-fine in-depth structures at the sub-cellular level.

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      Mentions: Arnaud Dubois
    20. Optical tissue sectioning using full field optical coherence tomography

      Optical tissue sectioning using full field optical coherence tomography

      According to a first aspect, the invention relates to a multimodal optical sectioning microscope (200, 400, 600) for full-field imaging of a volumic and scattering sample comprising: --a full-field OCT system for providing an image of a first section in depth of the sample comprising an illumination sub-system (201, 401, 601) and a full-field imaging interferometer with a detection sub system (208, 408, 608) and an optical conjugation device for optically conjugating the sample and said detection sub system, wherein said optical conjugation device comprises a microscope objective (203, 403, 603), --a supplementary full-field optical sectioning imaging system for providing ...

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    21. Optical coherence microscopy of living cells and bioengineered tissue dynamics in high-resolution cross-section

      Optical coherence microscopy of living cells and bioengineered tissue dynamics in high-resolution cross-section

      Optical coherence tomography (OCT) is a valuable tool in the cross-sectional observation/analysis of three-dimensional (3-D) biological tissues, and that histological observation is important clinically. However, the resolution of the technology is approximately 10–20 μm. In this study, optical coherence microscopy (OCM), a tomographic system combining OCT technology with a microscopic technique, was constructed for observing cells individually with a resolution at the submicrometer level. Cells and 3-D tissues fabricated by cell sheet technology were observed by OCM. Importantly, the cell nuclei and cytoplasm could be clearly distinguished, and the time-dependent dynamics of cell-sheet tissues could be observed in ...

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      Mentions: Panasonic
    22. Treatment monitoring of 0.5% 5-fluorouracil and 10% salicylic acid in clinical and subclinical actinic keratoses with the combination of optical coherence tomography and reflectance confocal microscopy

      Treatment monitoring of 0.5% 5-fluorouracil and 10% salicylic acid in clinical and subclinical actinic keratoses with the combination of optical coherence tomography and reflectance confocal microscopy

      Background Reflectance confocal microscopy (RCM) and high-definition optical coherence tomography (HD-OCT) allow the observation in vivo of dynamic changes in response to non-surgical treatment of actinic keratosis and field of cancerisation. Objective To non-invasively assess the pharmacodynamic changes induced by treatment with low dose 5-fluorouracil and 10% salicylic acid by means of RCM and high-definition OCT in field cancerization and actinic keratosis. Methods Twenty patients aged >50 years with diagnosis of actinic keratosis on the head and face and indication for treatment with 0.5% 5-fluorouracil and 10% salicylic acid were enrolled. An area of 25 cm² including visible type ...

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    23. Image contrast reduction mechanism in full-field optical coherence tomography

      Image contrast reduction mechanism in full-field optical coherence tomography

      Correct interpretation of image contrast obtained with full-field optical coherence tomography (FFOCT) technique is required for accurate medical diagnosis applications. In this work, first, the characteristics of microscopic structures of tissue that generate the contrast in en-face tomographic image obtained with FFOCT are discussed. Then an overview is given of the parameters that affect image contrast. Finally, the contrast correction factor for correct image interpretation and the contrast limits to practical FFOCT systems are outlined.

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      Mentions: Wanrong Gao
    1-24 of 431 1 2 3 4 ... 16 17 18 »
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