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(4 articles) University of Houston
(3 articles) University of Kent
(3 articles) Case Western Reserve University
(2 articles) Simon Fraser University
(2 articles) University of British Columbia
(2 articles) Technical University of Denmark
(2 articles) Oregon Health & Science University
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Gene Deletion Screen for Cardiomyopathy in Adult Drosophila Identifies a New Notch Ligand
Explore Article Circulation Research (Mar 7 2010) Cardiology , Developmental Biology
Rationale: Drosophila has been recognized as a model to study human cardiac diseases. Objective: Despite these findings, and the wealth of tools that are available to the fly community, forward genetic screens for adult heart phenotypes have been rarely performed because of the difficulty in accurately measuring cardiac function in adult Drosophila. Methods and Results: Using optical coherence tomography to obtain real-time analysis of cardiac function in awake Drosophila, we performed a genomic deficiency screen in adult flies. Based on multiple complementary approaches, we identified CG31665 as a novel gene causing dilated cardiomyopathy. CG31665, which we name weary (wry), has ...
Comment on Article Mentions: Duke University Bioptigen
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Focusing light through living tissue
Explore Article SPIE Digital Library (Feb 19 2010) Developmental Biology , Microscopy
Tissues such as skin, fat or cuticle are non-transparent because inhomogeneities in the tissue scatter light. We demonstrate experimentally that light can be focused through turbid layers of living tissue, in spite of scattering. Our method is based on the fact that coherent light forms an interference pattern, even after hundreds of scattering events. By spatially shaping the wavefront of the incident laser beam, this interference pattern was modified to make the scattered light converge to a focus. In contrast to earlier experiments, where light was focused through solid objects, we focused light through living pupae of Drosophila melanogaster. We ...
Comment on Article Mentions: University of Zurich
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Minimal invasive localization of the germinal disc in ovo for subsequent chicken sexing using optical coherence tomography
Explore Article SPIE Digital Library (Feb 19 2010) Developmental Biology
Reason for using optical coherence tomography (OCT) to locate the germinal disc is the questionable and ethically alarming killing of male layer chickens because for the layer line only the females are necessary. To avoid this and to protect the animal rights, the sex of the fertilized chicken egg has to be determined as early as possible in the unincubated state. Because the information whether the chick becomes male or female can be found in the germinal disc an accurate localization for sexing is essential. The germinal disc is located somewhere on top of the yolk and has a diameter ...
Comment on Article Mentions: Edmund Koch Julia Walther Dresden University of Technology
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Gabor-based fusion technique for Optical Coherence Microscopy
Explore Article opticsinfobase.org (Feb 4 2010) Dermatology , Developmental Biology , Microscopy
We recently reported on an Optical Coherence Microscopy technique, whose innovation intrinsically builds on a recently reported - 2 μm invariant lateral resolution by design throughout a 2 mm cubic full-field of view - liquid-lens-based dynamic focusing optical probe [Murali et al.,Optics Letters 34, 145-147, 2009]. We shall report in this paper on the image acquisition enabled by this optical probe when combined with an automatic data fusion method developed and described here to produce an in-focus high resolution image throughout the imaging depth of the sample. An African frog tadpole (Xenopus laevis) was imaged with the novel probe and ...
Comment on Article Mentions: University of Rochester University of Central Florida Jannick P. Rolland
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Fourier Domain Optical Coherence Tomography as a Noninvasive Means for In Vivo Detection of Retinal Degeneration in Xenopus laevis Tadpoles
Explore Article iovs.org (Jan 19 2010) Developmental Biology , Ophthalmology
ose. To determine the efficacy of Fourier domain optical coherence tomography (FD-OCT) as a noninvasive, nonlethal method for detecting in vivo, pathologic signs of retinal degeneration in Xenopus laevis larvae. Methods. A prototype OCT system using FD detection customized for tadpole imaging was used to noninvasively obtain retinal scans in two different transgenic X. laevis models of retinal degeneration. FD-OCT retinal scans were compared with laser scanning confocal micrographs of histologic sections of the same eye. Retinal thickness was measured in the histologic micrographs and compared with in vivo measurements acquired with FD-OCT. Results. In vivo retinal images of X. ...Comment on Article Mentions: University of British Columbia Marinko V. Sarunic Simon Fraser University
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Development of a high speed spectral domain optical coherence tomography system at 835 nm
Explore Article SPIE Digital Library (Dec 1 2009) Developmental Biology , Doppler
We develop a high speed multi-functional spectral domain (SD-OCT) system, using a broadband light source centered at 835nm and a custom-built spectrometer with a spectral resolution of 0.0674nm. After precise spectral calibration of the spectrometer, non-uniform discrete Fourier transform (NDFT) of the acquired spectral data is adopted for image reconstruction. In vivo imaging of human finger from volunteer at A-scan rate of 29 kHz with different reconstruction methods reveal that with the NDFT method, improved sensitivity fall-off especially at large depth is achieved in contrast to the conventional discrete Fourier transform (DFT) with interpolation method. And the phase information of ...Comment on Article Mentions: Zhejiang University
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Multiple-cardiac-cycle noise reduction in dynamic optical coherence tomography of the embryonic heart and vasculature
Explore Article opticsinfobase.org (Nov 20 2009) Cardiology , Developmental Biology
Recent progress in optical coherence tomography (OCT) allows imaging dynamic structures and fluid flow within scattering tissue, such as the beating heart and blood flow in mouse embryos. Accurate representation and analysis of these dynamic behaviors require reducing the noise of the acquired data. Although noise can be reduced by averaging multiple neighboring pixels in space or time, such operations reduce the effective spatial or temporal resolution that can be achieved. We have developed a computational postprocessing technique to restore image sequences of cyclically moving structures that preserves frame rate and spatial resolution. The signal-to-noise ratio (SNR) is improved by ...
Comment on Article Mentions: Kirill V. Larin Baylor College of Medicine University of Houston
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In vivo imaging of the cyclic changes in cross-sectional shape of the ventricular segment of pulsating embryonic chick hearts at stages 14 to 17: A contribution to the understanding of the ontogenesis of cardiac pumping function
Explore Article www3.interscience.wiley.com (Nov 20 2009) Developmental Biology
The cardiac cycle-related deformations of tubular embryonic hearts were traditionally described as concentric narrowing and widening of a tube of circular cross-section. Using optical coherence tomography (OCT), we have recently shown that, during the cardiac cycle, only the myocardial tube undergoes concentric narrowing and widening while the endocardial tube undergoes eccentric narrowing and widening, having an elliptic cross-section at end-diastole and a slit-shaped cross-section at end-systole. Due to technical limitations, these analyses were confined to early stages of ventricular development (chick embryos, stages 10-13). Using a modified OCT-system, we now document, for the first time, the cyclic changes in cross-sectional ...Comment on Article Mentions: Technical University of Denmark Lars Thrane
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Manual-scanning optical coherence tomography probe based on position tracking
Explore Article opticsinfobase.org (Oct 27 2009) Developmental Biology , Probes
A method based on position tracking to reconstruct images for a manual-scanning optical coherence tomography (OCT) probe is proposed and implemented. The method employs several feature points on a hand-held probe and a camera to track the device’s pose. The continuous device poses tracking, and the collected OCT depth scans can then be combined to render OCT images. The tracking accuracy of the system was characterized to be about 6 um along two axes and 19 um along the third. A phantom target was used to validate the method. In addition, we report OCT images of a 54-stage Xenopus laevis ...
Comment on Article Mentions: Jigang Wu Jian Ren Emily J. McDowell
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A 3D Video of the Embryonic Heartbeat
Explore Article Technology Review (Oct 22 2009) Cardiology , Developmental Biology
Researchers are using a new imaging technique to study the development of the mammalian heart. One percent of infants in the United States are born with cardiovascular abnormalities. The developmental processes that lead to these congenital problems aren't visible in ultrasound scans, and the lack of tools for imaging mammalian development non-invasively at high resolution has hindered researchers' attempts to understand these processes. In the hopes of providing insights into how these developmental problems might be prevented, researchers at the University of Houston have developed an imaging system they're using to take 3D video of the mammalian heart as it ...Comment on Article Mentions: Kirill V. Larin University of Houston
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Live imaging of rat embryos with Doppler swept-source optical coherence tomography
Explore Article Scitation (Oct 1 2009) Developmental Biology , Doppler
The rat has long been considered an excellent system to study mammalian embryonic cardiovascular physiology, but has lacked the extensive genetic tools available in the mouse to be able to create single gene mutations. However, the recent establishment of rat embryonic stem cell lines facilitates the generation of new models in the rat embryo to link changes in physiology with altered gene function to define the underlying mechanisms behind congenital cardiovascular birth defects. Along with the ability to create new rat genotypes there is a strong need for tools to analyze phenotypes with high spatial and temporal resolution. Doppler OCT ...Comment on Article Mentions: Kirill V. Larin University of Houston
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Serial Examination of an Inducible and Reversible Dilated Cardiomyopathy in Individual Adult Drosophila
Explore Article PLoS ONE (Sep 24 2009) Developmental Biology
Recent work has demonstrated that Drosophila can be used as a model of dilated cardiomyopathy, defined as an enlarged cardiac chamber at end-diastole when the heart is fully relaxed and having an impaired systolic function when the heart is fully contracted. Gene mutations that cause cardiac dysfunction in adult Drosophila can result from abnormalities in cardiac development or alterations in post-developmental heart function. To clarify the contribution of transgene expression to post-developmental cardiac abnormalities, we applied strategies to examine the temporal and spacial effects of transgene expression on cardiac function. We engineered transgenic Drosophila based on the well-characterized temperature-sensitive Gal80 ...Comment on Article Mentions: Duke University Bioptigen
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Doppler OCT imaging of cytoplasm shuttle flow in Physarum polycephalum
Explore Article www3.interscience.wiley.com (Sep 12 2009) Developmental Biology , Doppler
The Doppler optical coherence tomography technique was applied to image the oscillatory dynamics of protoplasm in the strands of the plasmodium of slime mould Physarum polycephalum. Radial contractions of the gel-like walls of the strands and the velocity distributions in the sol-like endoplasm streaming along the plasmodial strands are imaged. The motility inhibitor effect of carbon dioxide on the cytoplasm shuttle flow and strand-wall contraction is shown. The optical attenuation coefficient of cytoplasm is estimated.Comment on Article Mentions: Risto A. Myllylä University of Oulu Alexander V. Bykov
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Fourier domain optical coherence tomography (FD-OCT) as a non-invasive means for in vivo detection of retinal degeneration in Xenopus laevis tadpoles.
Explore Article iovs.org (Sep 10 2009) Developmental Biology , Ophthalmology
Purpose: To determine the efficacy of Fourier domain-optical coherence tomography (FD-OCT) as a non-invasive and non-sacrificial method for detecting in vivo, pathological signs of retinal degeneration in Xenopus laevis larvae. Methods: A prototype OCT system using Fourier domain detection customized for tadpole imaging was used to non-invasively obtain retinal scans in two different transgenic X. laevis models of retinal degeneration. FD-OCT retinal scans were compared with laser scanning confocal micrographs of histological sections of the same eye. Retinal thickness was measured in the histological micrographs and compared with in vivo measurements acquired with FD-OCT. Results: We obtained in vivo retinal ...Comment on Article Mentions: University of British Columbia Marinko V. Sarunic Simon Fraser University
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Ultra-high-resolution full-field optical coherence tomography for imaging of a developing embryo
Explore Article SPIE Digital Library (Aug 29 2009) Developmental Biology , Microscopy
Optical coherence tomography (OCT) is a new emerging technique for cross-sectional imaging with high spatial resolution of micrometer scale. It enables in vivo and non-invasive imaging with no need to contact the sample and is widely used in biological and clinic application. In this paper a white-light interference microscope is developed for ultrahigh-resolution full-field optical coherence tomography (Full-Field OCT) to implement 3D imaging of biological tissue. The experimental setup is based on a Linnik-type interferometer illuminated by a tungsten halogen lamp via a bundle of fiber. En-face tomographic images are obtained by demodulation of a combination of interferometric images recorded ...
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Dear Prof. Dr. Gary S. Mintz, You are an extraordinary expert in imaging, an intelligence ...
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