1. Articles from D. J. Faber

    1-16 of 16
    1. En‐face optical coherence tomography for the detection of cancer in prostatectomy specimens: quantitative analysis in 20 patients

      En‐face optical coherence tomography for the detection of cancer in prostatectomy specimens: quantitative analysis in 20 patients

      The increase histopathological evaluation of prostatectomy specimens rises the workload on pathologists. Automated histopathology systems, preferably directly on unstained specimens, would accelerate the pathology workflow. In this study, we investigate the potential of quantitative analysis of Optical Coherence Tomography (OCT) to separate benign from malignant prostate tissue automatically. Twenty fixated prostates were cut, from which 54 slices were scanned by OCT. Quantitative OCT metrics (attenuation coefficient, residue, goodness‐of‐fit) were compared for different tissue types, annotated on the histology slides. To avoid misclassification, the poor‐quality slides, and edges of annotations were excluded. Accurate registration of OCT data with ...

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    2. One‐to‐one registration of en‐face optical coherence tomography attenuation coefficients with histology of a prostatectomy specimen

      One‐to‐one registration of en‐face optical coherence tomography attenuation coefficients with histology of a prostatectomy specimen

      Optical coherence tomography (OCT), enables high‐resolution 3D imaging of the morphology of light scattering tissues. From the OCT signal, parameters can be extracted and related to tissue structures. One of the quantitative parameters is the attenuation coefficient; the rate at which the intensity of detected light decays in depth. To couple the quantitative parameters with the histology one‐to‐one registration is needed. The primary aim of this study is to validate a registration method of quantitative OCT parameters to histological tissue outcome through one‐to‐one registration of OCT with histology. We matched OCT images of unstained fixated ...

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    3. Optical Coherence Tomography as a Tool for in Vivo Staging and Grading of Upper Urinary Tract Urothelial Carcinoma: A Study for Diagnostic Accuracy

      Optical Coherence Tomography as a Tool for in Vivo Staging and Grading of Upper Urinary Tract Urothelial Carcinoma: A Study for Diagnostic Accuracy

      Purposes Biopsies and cytology are cornerstones in the diagnosis of upper urinary tract urothelial carcinoma (UTUC). However, a high rate of non-diagnostic biopsies, tumor upgrading and -staging after nephroureterectomy is observed. In this prospective in vivo study we aimed to evaluate the diagnostic accuracy of OCT for grading and staging of UTUC Material & Methods Twenty-six patients underwent diagnostic URS including biopsies and OCT imaging, followed by nephroureterectomy or segmental ureter resection. Sensitivity, specificity, negative predictive value and positive predictive value of UTUC grading and staging by OCT were evaluated according to the STARD initiative and second stage (2a and 2b ...

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    4. Prostate cancer diagnosis by optical coherence tomography: First results from a needle based optical platform for tissue sampling

      Prostate cancer diagnosis by optical coherence tomography: First results from a needle based optical platform for tissue sampling

      The diagnostic accuracy of Optical Coherence Tomography (OCT) based optical attenuation coefficient analysis is assessed for the detection of prostate cancer. Needle-based OCT-measurements were performed on the prostate specimens. Attenuation coefficients were determined by an earlier described in-house developed software package. The mean attenuation coefficients (benign OCT data; malignant OCT data; p-value Mann-Whitney U test) were: (3.56 mm –1 ; 3.85 mm –1 ; p < 0.0001) for all patients combined. The area under the ROC curve was 0.64. In order to circumvent the effect of histopathology mismatching, we performed a sub-analysis on only OCT data in which tumor ...

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    5. Customized Tool for the Validation of Optical Coherence Tomography in Differentiation of Prostate Cancer

      Customized Tool for the Validation of Optical Coherence Tomography in Differentiation of Prostate Cancer

      Objective: To design and demonstrate a customized tool to generate histologic sections of the prostate that directly correlate with needle-based optical coherence tomography pullback measurements. Materials and Methods: A customized tool was created to hold the prostatectomy specimens during optical coherence tomography measurements and formalin fixation. Using the tool, the prostate could be sliced into slices of 4 mm thickness through the optical coherence tomography measurement trajectory. In this way, whole-mount pathology slides were produced in exactly the same location as the optical coherence tomography measurements were performed. Full 3-dimensional optical coherence tomography pullbacks were fused with the histopathology slides ...

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    6. Volumetric laser endomicroscopy in Barrett's esophagus: a feasibility study on histological correlation

      Volumetric laser endomicroscopy in Barrett's esophagus: a feasibility study on histological correlation

      Volumetric laser endomicroscopy (VLE) is a novel balloon-based optical coherence tomography (OCT) imaging technique that may improve detection of early neoplasia in Barrett's esophagus (BE). Most OCT studies lack a direct correlation between histology and OCT images. The aim is to investigate the optimal approach for achieving one-to-one correlation of ex-vivo VLE images of endoscopic resection (ER) specimens with histology. BE patients with and without early neoplasia underwent ER after delineating areas with electrocoagulation markers (ECM). After ER, specimens underwent additional ex-vivo marking with several different markers (ink, pin, Gold Probe) followed by ex-vivo VLE scanning. ER specimens were ...

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    7. Functional optical coherence tomography of pigmented lesions

      Functional optical coherence tomography of pigmented lesions

      Background Cutaneous melanomas are diagnosed worldwide in 231 130 patients per year. The sensitivity and specificity of melanoma diagnosis expresses the need for an additional diagnostic method. Optical coherence tomography (OCT) has shown that it allows morphological (qualitative) description of image features and quantitative analysis of pathology related light scattering by means of the attenuation coefficient (μ oct ). Objective We hypothesize that OCT images of nevi will differ qualitatively and quantitatively from melanomas. Methods Forty lesions from 33 consecutive patients were imaged with OCT. After data acquisition, excision was performed. Epidermal layer thickness was measured and values of μ oct were extracted ...

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    8. Video Interview on Optical coherence tomography as a tool for in vivo staging and grading of upper urinary tract urothelial cell carcinoma: Comparison with biopsies and histopathology of the resected specimen

      Video Interview on Optical coherence tomography as a tool for in vivo staging and grading of upper urinary tract urothelial cell carcinoma: Comparison with biopsies and histopathology of the resected specimen

      Introduction and Objectives: Optical Coherence Tomography (OCT) has potential to provide real-time information on UUT-UC grade and stage. In this study we compare a) biopsy and b) OCT with gold standard histopathology of the resected specimen (HRS) for grading and staging of low- and high-grade UUT-UC. Methods: 16 patients with UUT-UC underwent diagnostic URS, including OCT and biopsy of places with visible tumour. The 2.7 Fr OCT probe was delivered through the ureteroscopes work channel. Subsequently biopsy was performed. At a later stage, patients underwent nephroureterectomy. For staging by OCT, in-house developed protocols were used and visible lesions were ...

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    9. Functional optical coherence tomography of pigmented lesions

      Functional optical coherence tomography of pigmented lesions

      Background Cutaneous melanomas are diagnosed worldwide in 231 130 patients per year. The sensitivity and specificity of melanoma diagnosis expresses the need for an additional diagnostic method. Optical coherence tomography (OCT) has shown that it allows morphological (qualitative) description of image features and quantitative analysis of pathology related light scattering by means of the attenuation coefficient (μ oct ). Objective We hypothesize that OCT images of nevi will differ qualitatively and quantitatively from melanomas. Methods Forty lesions from 33 consecutive patients were imaged with OCT. After data acquisition, excision was performed. Epidermal layer thickness was measured and values of μ oct were extracted ...

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    10. Dependent and multiple scattering in transmission and backscattering optical coherence tomography

      Dependent and multiple scattering in transmission and backscattering optical coherence tomography

      We use transmission and backscattering optical coherence tomography (OCT) to distinguish and quantify dependent and multiple scattering effects in turbid media. With transmission OCT the dependent scattering coefficients for a range of monodisperse silica particle suspensions are determined. An excellent agreement is observed between the measured dependent scattering coefficients and calculations based on Mie calculations, the Percus-Yevick radial distribution function, and coherent light scattering theory. Backscattering OCT measurements are fitted using the extended Huygens-Fresnel (EHF) model with the dependent scattering coefficients obtained from the transmission OCT measurements as input parameters. Good agreement between the EHF model and the backscattering OCT ...

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    11. Volumetric In-Vivo Visualization of Upper Urinary Tract Tumors Using Optical Coherence Tomography: A Pilot Study

      Volumetric In-Vivo Visualization of Upper Urinary Tract Tumors Using Optical Coherence Tomography: A Pilot Study

      Purpose Knowledge on tumor stage and grade is paramount for treatment decision in Upper Urinary Tract Urothelial Carcinoma (UUT-UC), but cannot be accurately assessed by current techniques. Optical Coherence Tomography (OCT) is a technique, which can hypothetically provide the urologist with real-time intra-operative information on tumor grade and stage. In this pilot study the first results of OCT in grading and staging of UUT-UC are presented. Material & Methods Eight consecutive patients underwent URS for UUT-UC suspicion or follow-up. OCT datasets were intra-operatively obtained from the ureter and pyelum. All patients eventually underwent nephroureterectomy. OCT staging was performed by visual inspection ...

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    12. Optical biopsy of epithelial cancers by optical coherence tomography (OCT)

      Optical biopsy of epithelial cancers by optical coherence tomography (OCT)

      Optical coherence tomography (OCT) is an optical technique that measures the backscattering of near-infrared light by tissue. OCT yields in 2D and 3D images at micrometer-scale resolution, thus providing optical biopsies, approaching the resolution of histopathological imaging. The technique has shown to allow in vivo differentiation between benign and malignant epithelial tissue, through qualitative assessment of OCT images, as well as by quantitative evaluation, e.g., functional OCT. This study aims to summarize the principles of OCT and to discuss the current literature on the diagnostic value of OCT in the diagnosis of epithelial (pre)malignant lesions. The authors did ...

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    13. Determination of the scattering anisotropy with optical coherence tomography

      Determination of the scattering anisotropy with optical coherence tomography
      In this work we demonstrate measurements with optical coherence tomography (OCT) of the scattering phase function in the backward direction and the scattering anisotropy parameter g. Measurements of the OCT attenuation coefficient and the backscattering amplitude are performed on calibrated polystyrene microspheres with a time-domain OCT system. From these measurements the phase function in the backward direction is determined. The measurements are described by the single scattering model and match Mie calculations very well. Measurements on Intralipid demonstrate the ability to determine the g of polydisperse samples and, for Intralipid, g = 0.35 ± 0.03 is measured, which is well ...
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    14. Quantitative comparison of the OCT imaging depth at 1300 nm and 1600 nm

      Quantitative comparison of the OCT imaging depth at 1300 nm and 1600 nm
      One of the present challenges in optical coherence tomography (OCT) is the visualization of deeper structural morphology in biological tissues. Owing to a reduced scattering, a larger imaging depth can be achieved by using longer wavelengths. In this work, we analyze the OCT imaging depth at wavelengths around 1300 nm and 1600 nm by comparing the scattering coefficient and OCT imaging depth for a range of Intralipid concentrations at constant water content. We observe an enhanced OCT imaging depth for 1600 nm compared to 1300 nm for Intralipid concentrations larger than 4 vol.%. For higher Intralipid concentrations, the imaging depth ...
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    15. Multiple and dependent scattering effects in Doppler optical coherence tomography

      Multiple and dependent scattering effects in Doppler optical coherence tomography
      Doppler optical coherence tomography (OCT) is a technique to image tissue morphology and to measure flow in turbid media. In its most basic form, it is based on single (Mie) scattering. However, for highly scattering and dense media multiple and concentration dependent scattering can occur. For Intralipid solutions with varying scattering strength, the effect of multiple and dependent scattering on the OCT signal attenuation and Doppler flow is investigated. We observe a non-linear increase in the OCT signal attenuation rate and an increasingly more distorted Doppler OCT flow profile with increasing Intralipid concentration. The Doppler OCT attenuation and flow measurements ...
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    16. Influence of cataract on optical coherence tomography image quality and retinal thickness

      Background: As optical coherence tomography (OCT) is widely used for diagnosis and monitoring of ocular pathology, especially in the elderly people, the influence of cataract on image quality and macular retinal thickness was studied. Methods: In 29 patients scheduled for cataract surgery, preoperative and postoperative OCT scans were obtained. Cataracts were categorised as nuclear, posterior or cortical. Parameters for image quality (signal-to-noise ratio (SNR)) and signal strength and macular thickness were compared. A three-level expert grading scale was used to evaluate the discriminative abilities of SNR and signal strength. Results: Nuclear cataracts (n = 12) provided better preoperative scans (higher SNR ...

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    1-16 of 16
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  2. Topics in the News

    1. (16 articles) Dirk J. Faber
    2. (15 articles) Ton G. van Leeuwen
    3. (14 articles) Academic Medical Center at the University of Amsterdam
    4. (11 articles) Daniël M. de Bruin
    5. (5 articles) Jean J. M. C. H. de la Rosette
    6. (4 articles) University of Twente
    7. (4 articles) VU University Amsterdam
    8. (4 articles) Theo M. de Reijke
    9. (4 articles) Jeroen Kalkman
    10. (3 articles) Mieke T. J. Bus
    11. (1 articles) Sun Yat-Sen University
    12. (1 articles) National Institutes of Health
    13. (1 articles) University of Illinois at Urbana-Champaign
    14. (1 articles) Tianjin University
    15. (1 articles) Zhejiang University
    16. (1 articles) New York Eye and Ear Infirmary
    17. (1 articles) Eric J. Chaney
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    Multiple and dependent scattering effects in Doppler optical coherence tomography Quantitative comparison of the OCT imaging depth at 1300 nm and 1600 nm Determination of the scattering anisotropy with optical coherence tomography Optical biopsy of epithelial cancers by optical coherence tomography (OCT) Volumetric In-Vivo Visualization of Upper Urinary Tract Tumors Using Optical Coherence Tomography: A Pilot Study Video Interview on Optical coherence tomography as a tool for in vivo staging and grading of upper urinary tract urothelial cell carcinoma: Comparison with biopsies and histopathology of the resected specimen Volumetric laser endomicroscopy in Barrett's esophagus: a feasibility study on histological correlation Customized Tool for the Validation of Optical Coherence Tomography in Differentiation of Prostate Cancer Prostate cancer diagnosis by optical coherence tomography: First results from a needle based optical platform for tissue sampling Optical coherence tomography angiography in glaucoma Handheld optical coherence tomography for clinical assessment of dental plaque and gingiva Characterisation of macular superficial vessel density alteration in preclinical ethambutol-induced optic neuropathy using optical coherence tomography angiography