1. Articles from Ruchire Eranga Wijesinghe

    1-22 of 22
    1. Identification of multi-dimensional thread geometry using depth-resolved swept-source optical coherence tomography for assessment of dental implant fabrication

      Identification of multi-dimensional thread geometry using depth-resolved swept-source optical coherence tomography for assessment of dental implant fabrication

      The geometrical characteristics of dental implants, such as thread depth and width, facing angle, pitch, and surface roughness, are important to assess their stability and osseointegration after implant surgery. Herein, we demonstrate the potential use of depth-resolved swept-source optical coherence tomography (SS-OCT) to assess the structural quality of widely used dental implants. The implemented SS-OCT system was centered at a wavelength of 1300 nm with a 100 nm full-width at half-maximum. Four dental implants with different structural formations fabricated using either titanium or ceramic were visualized. Qualitative assessments were performed using boundary flattening with an amplitude-profiling algorithm to emphasize and ...

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    2. An averaged intensity difference detection algorithm for identification of human gingival sulcus in optical coherence tomography images

      An averaged intensity difference detection algorithm for identification of human gingival sulcus in optical coherence tomography images

      In the past decade, there has been an increase in the development of sensitive, highresolution, non-invasive diagnostic methods for periodontic diseases. Optical coherence tomography (OCT) has attracted considerable attention in clinical settings. In this study, a reliable, robust algorithm for the detection of gingival sulcus in 2D OCT cross-sectional images is proposed. Previously, the measurement of gingival sulcus in OCT images has been performed by manual identification using two-dimensional (2D) cross-sectional images. The automated detection of gingival sulcus continuity in 2D OCT images may help medical practitioners to assess important features of gingival tissues. The Sobel and canny operators have ...

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    3. Non-ionized, High-resolution Measurement of Internal and Marginal Discrepancies of Dental Prosthesis Using Optical Coherence Tomography

      Non-ionized, High-resolution Measurement of Internal and Marginal Discrepancies of Dental Prosthesis Using Optical Coherence Tomography

      An internal and marginal fit between prosthesis and abutment are important factors of the durability of dental prosthesis. In this study we have proposed the use of swept-source optical coherence tomography (SS-OCT) as a novel application for non-ionized and high-resolution measurements of internal and marginal discrepancies at anatomically critical four points, such as occlusal, angle, axial, and margin during prosthesis attachment. A tooth model was fabricated by 3D printing technique and the dental prosthesis was designed using dental CAD software. The cross-sectional images along with intensity peak profile analysis of the sample were acquired using OCT system for measurements of ...

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    4. Clinical Utility of Intraoperative Tympanomastoidectomy Assessment Using a Surgical Microscope Integrated with an Optical Coherence Tomography

      Clinical Utility of Intraoperative Tympanomastoidectomy Assessment Using a Surgical Microscope Integrated with an Optical Coherence Tomography

      Significant technical and optical advances are required for intraoperative optical coherence tomography (OCT) to be utilized during otological surgeries. Integrating OCT with surgical microscopy makes it possible to evaluate soft tissue in real-time and at a high resolution. Herein, we describe an augmented-reality, intraoperative OCT/microscope system with an extended working distance of 280 mm, providing more space for surgical manipulation than conventional techniques. We initially performed ex vivo experiments to evaluate system performance. In addition, we validated the system by performing preliminary clinical assessments of tympanomastoidectomy outcomes in six patients with chronic otitis media. The system evaluated residual inflammation ...

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    5. Non-destructive identification of weld-boundary and porosity formation during laser transmission welding by using optical coherence tomography

      Non-destructive identification of weld-boundary and porosity formation during laser transmission welding by using optical coherence tomography

      Laser transmission welding offers significant benefits over conventional welding techniques enabling single-stage rapid plastic joining. The quality of laser transmission welded products are commonly assessed by measuring the weld penetration depth, hardened weld boundary, and inspecting the formation of porosity. However, existing methods of verification are inevitably accompanied by destruction of the specimen. Thus, non-destructive quality assessment methods for laser transmission welding have gained attention recently. Here, we demonstrated an extended industrial application of 860 nm wavelength based spectral domain optical coherence tomography for the non-destructive inspection of the aforementioned quality parameters of laser transmission welded industrial plastic materials, i ...

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    6. Biophotonic approach for the characterization of initial bitter-rot progression on apple specimens using optical coherence tomography assessments

      Biophotonic approach for the characterization of initial bitter-rot progression on apple specimens using optical coherence tomography assessments

      The tremendous advances achieved in the biophotonics technologies have intensified the necessity for non-invasive modalities that can characterize diverse biological materials with increased sensitivity and resolution. Optical coherence tomography (OCT) is one of the techniques that has been applied for biological applications in medicine and agriculture to identify structural properties. Herein, we report the successful incorporation of OCT for the identification of morphological changes that occur as a result of the bitter rot disease, through continuous detection of structural changes. Detailed inner morphological structural changes occurring in fruit specimens were precisely analyzed as a function of the disease incubation period ...

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    7. Quality assessment of the optical thin films using line field spectral domain optical coherence tomography

      Quality assessment of the optical thin films using line field spectral domain optical coherence tomography

      Cross-sectional and three-dimensional images show the effectiveness of the system. In this study, the industrial inspection of optical thin film on touch screen panels was demonstrated using line field spectral domain optical coherence tomography. The conventional Fourier domain optical coherence tomography system requires a single scanner for two-dimensional cross-sectional images and two scanners for volumetric images. Our developed line field spectral domain optical coherence tomography has the advantage of needing only a single scanner for volumetric images, while two-dimensional cross-sectional images are obtained by the parallel acquisition of an illuminated line on a sample using an area camera. Further, the ...

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    8. In Vivo Non-Destructive Monitoring of Capsicum Annuum Seed Growth with Diverse NaCl Concentrations Using Optical Detection Technique

      In Vivo Non-Destructive Monitoring of Capsicum Annuum Seed Growth with Diverse NaCl Concentrations Using Optical Detection Technique

      We demonstrate that optical coherence tomography (OCT) is a plausible optical tool for in vivo detection of plant seeds and its morphological changes during growth. To investigate the direct impact of salt stress on seed germination, the experiment was conducted using Capsicum annuum seeds that were treated with different molar concentrations of NaCl. To determine the optimal concentration for the seed growth, the seeds were monitored for nine consecutive days. In vivo two-dimensional OCT images of the treated seeds were obtained and compared with the images of seeds that were grown using sterile distilled water. The obtained results confirm the ...

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    9. The Validity of the Diagnosis of Plant Leaf Infections Using Non-Destructive Optical Inspection Technique and Depth-Scan Signal Analysis

      The Validity of the Diagnosis of Plant Leaf Infections Using Non-Destructive Optical Inspection Technique and Depth-Scan Signal Analysis

      The applicability of non-destructive optical imaging technique for the diagnosis of infected leaf materials was investigated along with a depth-scan (amplitude depth profile) signal analysis. The experimented plant leaf specimens were collected from persimmon plantations and the specimens were categorized into three groups: healthy leaf specimens, infected leaf specimens, and apparently healthy leaf specimens collected from infected trees. The non-destructive diagnosis was performed using 840 nm spectral domain optical coherence tomography (SD-OCT) system. The obtained OCT results confirmed that the proposed method is capable of identifying morphological differences between healthy specimens from infected specimens and partially infected specimens. Therefore, this ...

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    10. Feature Of The Week 09/17/2017: Non-Destructive Analysis of the Internal Anatomical Structures of Mosquito Specimens Using Optical Coherence Tomography

      Feature Of The Week 09/17/2017: Non-Destructive Analysis of the Internal Anatomical Structures of Mosquito Specimens Using Optical Coherence Tomography

      The study of mosquitoes and analysis of their behavior are of crucial importance in the on-going efforts to control the alarming increase in mosquito-borne diseases. Furthermore, a non-destructive and real-time imaging technique to study the anatomical features of mosquito specimens can greatly aid the study of mosquitoes. In this study, we demonstrate the three-dimensional imaging capabilities of optical coherence tomography (OCT) for structural analysis of Anopheles sinensis mosquitoes. The anatomical features of An. sinensis head, thorax, and abdominal regions, along with the morphology of internal structures, such as foregut, midgut, and hindgut, were studied using OCT imaging. Two-dimensional and three-dimensional ...

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    11. Application of wearable optical coherence tomography (OCT) and Loop-mediated isothermal amplification (LAMP) techniques for In situ real-time field inspection of apple Marssonina blotch disease

      Application of wearable optical coherence tomography (OCT) and Loop-mediated isothermal amplification (LAMP) techniques for In situ real-time field inspection of apple Marssonina blotch disease

      Here we describe the possible application of optical coherence tomography (OCT) to inspect Marssonina coronaria infected apple blotch disease of in situ apple leaves. To fulfill the in situ field inspection requirement, we developed a compact wearable OCT system. For the confirmation of OCT results, simultaneous experiment was performed in realtime using loop-mediated isothermal amplification (LAMP), which is frequently used in agriculture. LAMP method was developed as an alternative approach for the inspection of disease. We performed field inspection for 30 consecutive days, and all the acquired results from both OCT and lamp were compared to confirm the correlation. A ...

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    12. Dual illumination for cornea and retina imaging using spectral domain optical coherence tomography

      Dual illumination for cornea and retina imaging using spectral domain optical coherence tomography

      A dual illumination system is proposed for cornea and retina imaging using spectral domain optical coherence tomography (SD-OCT). The system is designed to acquire cornea and retina imaging with dual illumination with limited optics and using a single spectrometer. The beam propagation for cornea and retina imaging in dual illumination enables to acquire the images of different segments. This approach will reduce the imaging time for separate corneal and retinal imaging. The in vivo imaging of both the cornea and retina of a health volunteer shows the feasibility of the system for clinical applications.

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    13. In vivo observation of metamorphosis of Plodia interpunctella Hübner using three-dimensional optical coherence tomography

      In vivo observation of metamorphosis of Plodia interpunctella Hübner using three-dimensional optical coherence tomography

      Morphological assessment and three-dimensional reconstructions of internal structures of Plodia interpunctella Hübner during metamorphosis stages were experimentally demonstrated using optical coherence tomography (OCT) for the first time. The conventional, complex sectioning methods were significantly simplified owing to the non-invasive three-dimensional imaging capability of OCT. Further, this study demonstrates the use of OCT as a non-invasive detection tool for in vivo morphological observation of metamorphosis stages to gain a better understanding about the growth of internal organs, which can be considered a useful discovery in the field of entomology. Thus, the metamorphosis stages starting from the larva, three pupa stages ...

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    14. Optical coherence tomography-integrated, wearable (backpack-type), compact diagnostic imaging modality for in situ leaf quality assessment

      Optical coherence tomography-integrated, wearable (backpack-type), compact diagnostic imaging modality for in situ leaf quality assessment

      We developed a compact, wearable diagnostic imaging modality employing optical coherence tomography for in situ plant leaf quality assessments. This system is capable of diagnosing infected leaves at the initial disease stages. Our system is a versatile backpack-type imaging modality with a compact spectrometer, miniature computer, rechargeable power source, and handheld inspection probe. This method enhances real-time in situ specimen inspection through direct implementation of the imaging modality in a plantation. To evaluate the initial performance, field experiments were conducted in apple, pear, and persimmon plantations. Based on the obtained results, we can conclude that the developed imaging modality can ...

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    15. Optical sensing method to analyze germination rate of Capsicum annum seeds treated with growth-promoting chemical compounds using optical coherence tomography

      Optical sensing method to analyze germination rate of Capsicum annum seeds treated with growth-promoting chemical compounds using optical coherence tomography

      Seed germination rate differs based on chemical treatments, and nondestructive measurements of germination rate have become an essential requirement in the field of agriculture. Seed scientists and other biologists are interested in optical sensing technologies-based biological discoveries due to nondestructive detection capability. Optical coherence tomography (OCT) has recently emerged as a powerful method for biological and plant material discoveries. We report an extended application of OCT by monitoring the germination rate acceleration of chemically primed seeds. To validate the versatility of the method, Capsicum annum seeds were primed using three chemical compounds: sterile distilled water (SDW), butandiol, and 1-hexadecene. Monitoring ...

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    16. Dual-path handheld system for cornea and retina imaging using optical coherence tomography

      Dual-path handheld system for cornea and retina imaging using optical coherence tomography

      A dual-path handheld system is proposed for cornea and retina imaging using spectral domain optical coherence tomography. The handheld sample arm is designed to acquire two images simultaneously. Both eyes of a person can be imaged at the same time to obtain the images of the cornea of one eye and the retina of the other eye. Cornea, retina, and optic disc images are acquired with the proposed sample arm. Experimental results demonstrate the usefulness of this system for imaging of different eye segments. This system reduces the time required for imaging of the two eyes and is cost effective.

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    17. Fast Industrial Inspection of Optical Thin Film Using Optical Coherence Tomography

      Fast Industrial Inspection of Optical Thin Film Using Optical Coherence Tomography

      An application of spectral domain optical coherence tomography (SD-OCT) was demonstrated for a fast industrial inspection of an optical thin film panel. An optical thin film sample similar to a liquid crystal display (LCD) panel was examined. Two identical SD-OCT systems were utilized for parallel scanning of a complete sample in half time. Dual OCT inspection heads were utilized for transverse (fast) scanning, while a stable linear motorized translational stage was used for lateral (slow) scanning. The cross-sectional and volumetric images of an optical thin film sample were acquired to detect the defects in glass and other layers that are ...

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    18. Depth enhancement in spectral domain optical coherence tomography using bidirectional imaging modality with a single spectrometer

      Depth enhancement in spectral domain optical coherence tomography using bidirectional imaging modality with a single spectrometer

      A method for depth enhancement is presented using a bidirectional imaging modality for spectral domain optical coherence tomography (SD-OCT). Two precisely aligned sample arms along with two reference arms were utilized in the optical configuration to scan the samples. Using exemplary images of the optical resolution target, Scotch tape, a silicon sheet with two needles, and a leaf, we demonstrated how the developed bidirectional SD-OCT imaging method increases the ability to characterize depth-enhanced images. The results of the developed system were validated by comparing the images with the standard OCT configuration (single-sample arm setup). Given the advantages of higher resolution ...

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    19. In vivo imaging of melanoma-implanted magnetic nanoparticles using contrast-enhanced magneto-motive optical Doppler tomography

      In vivo imaging of melanoma-implanted magnetic nanoparticles using contrast-enhanced magneto-motive optical Doppler tomography

      We conducted an initial feasibility study using real-time magneto-motive optical Doppler tomography (MM-ODT) with enhanced contrast to investigate the detection of superparamagnetic iron oxide (SPIO) magnetic nanoparticles implanted into in vivo melanoma tissue. The MM-ODT signals were detected owing to the phase shift of the implanted magnetic nanoparticles, which occurred due to the action of an applied magnetic field. An amplifier circuit-based solenoid was utilized for generating high-intensity oscillating magnetic fields. The MM-ODT system was confirmed as an effective in vivo imaging method for detecting melanoma tissue, with the performance comparable to those of conventional optical coherence tomography and optical ...

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    20. Decalcification using ethylenediaminetetraacetic acid for clear microstructure imaging of cochlea through optical coherence tomography

      Decalcification using ethylenediaminetetraacetic acid for clear microstructure imaging of cochlea through optical coherence tomography

      The aim of this study was to analyze the effectiveness of decalcification using ethylenediaminetetraacetic acid (EDTA) as an optical clearing method to enhance the depth visibility of internal soft tissues of cochlea. Ex vivo mouse and guinea pig cochlea samples were soaked in EDTA solutions for decalcification, and swept source optical coherence tomography (OCT) was used as imaging modality to monitor the decalcified samples consecutively. The monitored noninvasive cross-sectional images showed that the mouse and guinea pig cochlea samples had to be decalcified for subsequent 7 and 14 days, respectively, to obtain the optimal optical clearing results. Using this method ...

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    21. Optically deviated focusing method based high-speed SD-OCT for in vivo retinal clinical applications

      Optically deviated focusing method based high-speed SD-OCT for in vivo retinal clinical applications

      The aim of this study is to provide accurately focused, high-resolution in vivo human retinal depth images using an optically deviated focusing method with spectral-domain optical coherence tomography (SD-OCT) system. The proposed method was applied to increase the retinal diagnosing speed of patients with various values of retinal distances (i.e., the distance between the crystalline eye lens and the retina). The increased diagnosing speed was facilitated through an optical modification in the OCT sample arm configuration. Moreover, the optical path length matching process was compensated using the proposed optically deviated focusing method. The developed system was mounted on a ...

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    22. Development of Real-Time Dual-Display Handheld and Bench-Top Hybrid-Mode SD-OCTs

      Development of Real-Time Dual-Display Handheld and Bench-Top Hybrid-Mode SD-OCTs

      Development of a dual-display handheld optical coherence tomography (OCT) system for retina and optic-nerve-head diagnosis beyond the volunteer motion constraints is reported. The developed system is portable and easily movable, containing the compact portable OCT system that includes the handheld probe and computer. Eye posterior chambers were diagnosed using the handheld probe, and the probe could be fixed to the bench-top cradle depending on the volunteers’ physical condition. The images obtained using this handheld probe were displayed in real time on the computer monitor and on a small secondary built-in monitor; the displayed images were saved using the handheld probe ...

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    1-22 of 22
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    Development of Real-Time Dual-Display Handheld and Bench-Top Hybrid-Mode SD-OCTs Optically deviated focusing method based high-speed SD-OCT for in vivo retinal clinical applications Decalcification using ethylenediaminetetraacetic acid for clear microstructure imaging of cochlea through optical coherence tomography Fast Industrial Inspection of Optical Thin Film Using Optical Coherence Tomography Dual-path handheld system for cornea and retina imaging using optical coherence tomography Optical sensing method to analyze germination rate of Capsicum annum seeds treated with growth-promoting chemical compounds using optical coherence tomography Optical coherence tomography-integrated, wearable (backpack-type), compact diagnostic imaging modality for in situ leaf quality assessment Application of wearable optical coherence tomography (OCT) and Loop-mediated isothermal amplification (LAMP) techniques for In situ real-time field inspection of apple Marssonina blotch disease Feature Of The Week 09/17/2017: Non-Destructive Analysis of the Internal Anatomical Structures of Mosquito Specimens Using Optical Coherence Tomography The Validity of the Diagnosis of Plant Leaf Infections Using Non-Destructive Optical Inspection Technique and Depth-Scan Signal Analysis ORCA study: real-world versus reading centre assessment of disease activity of neovascular age-related macular degeneration (nAMD) Role of visual evoked potentials and optical coherence tomography in the screening for optic pathway gliomas in patients with neurofibromatosis type I