1. Articles from Benquan Wang

    1-9 of 9
    1. Functional optical coherence tomography enables in vivo optoretinography of photoreceptor dysfunction due to retinal degeneration

      Functional optical coherence tomography enables in vivo optoretinography of photoreceptor dysfunction due to retinal degeneration

      Stimulus-evoked intrinsic optical signal (IOS), which occurs almost immediately after the onset of retinal stimulus has been observed in retinal photoreceptors, promises to be a unique biomarker for objective optoretinography (ORG) of photoreceptor function. We report here the first-time in vivo ORG detection of photoreceptor dysfunction due to retinal degeneration. A custom-designed optical coherence tomography (OCT) was employed for longitudinal ORG monitoring of photoreceptor-IOS distortions in retinal degeneration mice. Depth-resolved OCT analysis confirmed the outer segment (OS) as the physical source of the photoreceptor-IOS. Comparative ERG measurement verified the phototransduction activation as the physiological correlator of the photoreceptor-IOS. Histological examination ...

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    2. Functional optical coherence tomography of neurovascular coupling interactions in the retina

      Functional optical coherence tomography of neurovascular coupling interactions in the retina

      Quantitative evaluation of retinal neurovascular coupling is essential for a better understanding of visual function and early detection of eye diseases. However, there is no established method to monitor coherent interactions between stimulus‐evoked neural activity and hemodynamic responses at high resolution. Here we report a multi‐modal functional optical coherence tomography (OCT) imaging methodology to enable concurrent intrinsic optical signal (IOS) imaging of stimulus‐evoked neural activity and hemodynamic responses at capillary resolution. OCT angiography guided IOS analysis was used to separate neural‐IOS and hemodynamic‐IOS changes in the same retinal image sequence. Frequency flicker stimuli evoked neural ...

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    3. In vivo optical coherence tomography of stimulus-evoked intrinsic optical signals in mouse retinas

      In vivo optical coherence tomography of stimulus-evoked intrinsic optical signals in mouse retinas

      Intrinsic optical signal (IOS) imaging promises a noninvasive method for advanced study and diagnosis of eye diseases. Before pursuing clinical applications, it is essential to understand anatomic and physiological sources of retinal IOSs and to establish the relationship between IOS distortions and eye diseases. The purpose of this study was designed to demonstrate the feasibility of in vivo IOS imaging of mouse models. A high spatiotemporal resolution spectral domain optical coherence tomography (SD-OCT) was employed for depth-resolved retinal imaging. A custom-designed animal holder equipped with ear bar and bite bar was used to minimize eye movements. Dynamic OCT imaging revealed ...

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    4. Optical coherence tomography angiography of stimulus evoked hemodynamic responses in individual retinal layers

      Optical coherence tomography angiography of stimulus evoked hemodynamic responses in individual retinal layers

      Blood flow changes are highly related to neural activities in the retina. It has been reported that neural activity increases when flickering light stimulation of the retina is used. It is known that blood flow changes with flickering light stimulation can be altered in patients with vascular disease and that measurement of flicker-induced vasodilatation is an easily applied tool for monitoring functional microvascular alterations. However, details of distortions in retinal neurovascular coupling associated with major eye diseases are not well understood due to the limitation of existing techniques. In this study, flickering light stimulation was applied to mouse retinas to ...

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    5. In vivo intrinsic optical signal imaging of mouse retinas

      In vivo intrinsic optical signal imaging of mouse retinas

      Intrinsic optical signal (IOS) imaging is a promising noninvasive method for advanced study and diagnosis of eye diseases. Before pursuing clinical applications, more IOS studies employing animal models are necessary to establish the relationship between IOS distortions and eye diseases. Ample mouse models are available for investigating the relationship between IOS distortions and eye diseases. However, in vivo IOS imaging of mouse retinas is challenging due to the small ocular lens (compared to frog eyes) and inevitable eye movements. We report here in vivo IOS imaging of mouse retinas using a custom-designed functional OCT. The OCT system provided high resolution ...

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    6. Functional optical coherence tomography enables in vivo physiological assessment of retinal rod and cone photoreceptors

      Functional optical coherence tomography enables in vivo physiological assessment of retinal rod and cone photoreceptors

      Transient intrinsic optical signal (IOS) changes have been observed in retinal photoreceptors, suggesting a unique biomarker for eye disease detection. However, clinical deployment of IOS imaging is challenging due to unclear IOS sources and limited signal-to-noise ratios (SNRs). Here, by developing high spatiotemporal resolution optical coherence tomography (OCT) and applying an adaptive algorithm for IOS processing, we were able to record robust IOSs from single-pass measurements. Transient IOSs, which might reflect an early stage of light phototransduction, are consistently observed in the photoreceptor outer segment almost immediately (<4 ms) after retinal stimulation. Comparative studies of dark- and light-adapted retinas have ...

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    7. Functional optical coherence tomography reveals transient phototropic change of photoreceptor outer segments

      Functional optical coherence tomography reveals transient phototropic change of photoreceptor outer segments

      Dynamic near infrared microscopy has revealed transient retinal phototropism (TRP) correlated with oblique light stimulation. Here, by developing a hybrid confocal microscopy and optical coherence tomography (OCT), we tested sub-cellular source of the TRP in living frog retina. Dynamic confocal microscopy and OCT consistently revealed photoreceptor outer segments as the anatomic source of the TRP. Further investigation of the TRP can provide insights in better understanding of Stiles–Crawford effect (SCE) on rod and cone systems, and may also promise an intrinsic biomarker for early detection of eye diseases that can produce photoreceptor dysfunction.

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    8. Breaking diffraction limit of lateral resolution in optical coherence tomography

      Breaking diffraction limit of lateral resolution in optical coherence tomography

      Quantitative imaging of biomedical specimens is essential in biomedical study and diagnosis. Given excellent capability in three-dimensional (3D) imaging, optical coherence tomography (OCT) has been extensively used in ophthalmic imaging, vascular medicine, dermatological study, etc. Lateral resolution of the OCT is light diffraction limited, which precludes the feasibility of quantitative assessment of individual cells. In this paper, we demonstrated the feasibility of breaking diffraction-limit in OCT imaging through virtually structured detection (VSD). OCT examination of optical resolution target verified resolution doubling in the VSD based OCT imaging. Super-resolution OCT identification of individual frog photoreceptors was demonstrated to verify the potential ...

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    9. En face optical coherence tomography of transient light response at photoreceptor outer segments in living frog eyecup

      En face optical coherence tomography of transient light response at photoreceptor outer segments in living frog eyecup

      This study was designed to test the feasibility of en face mapping of the transient intrinsic optical signal (IOS) response at photoreceptor outer segments and to assess the effect of spatial resolution on functional IOS imaging of retinal photoreceptors. A line-scan optical coherence tomography (LS-OCT) was constructed to achieve depth-resolved functional IOS imaging of living frog eyecups. Rapid en face OCT revealed transient IOS almost immediately ( < 3     ms ) after the onset of visible light flashes at photoreceptor outer segments. Quantitative analysis indicated that the IOS kinetics may reflect dynamics of G-protein binding and releasing in early phases of visual transduction ...

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    1-9 of 9
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  2. Topics in the News

    1. (4 articles) University of Alabama
    2. (2 articles) Heidelberg Engineering
    3. (1 articles) Chinese Academy of Sciences
    4. (1 articles) Superlum
    5. (1 articles) Jichi Medical University
    6. (1 articles) Stanford University
    7. (1 articles) University of Florence
    8. (1 articles) Centre Hospitalier Intercommunal de Créteil
    9. (1 articles) University of Montreal
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    12. (1 articles) Carl Zeiss Meditec
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    En face optical coherence tomography of transient light response at photoreceptor outer segments in living frog eyecup Breaking diffraction limit of lateral resolution in optical coherence tomography Functional optical coherence tomography reveals transient phototropic change of photoreceptor outer segments Functional optical coherence tomography enables in vivo physiological assessment of retinal rod and cone photoreceptors In vivo intrinsic optical signal imaging of mouse retinas Optical coherence tomography angiography of stimulus evoked hemodynamic responses in individual retinal layers In vivo optical coherence tomography of stimulus-evoked intrinsic optical signals in mouse retinas Functional optical coherence tomography of neurovascular coupling interactions in the retina Functional optical coherence tomography enables in vivo optoretinography of photoreceptor dysfunction due to retinal degeneration The truth about invisible posterior vitreous structures The Influence of Eyelid Position and Environmental Conditions on the Corneal Changes in Early Postmortem Interval: A Prospective, Multicentric OCT Study The Use of Optical Coherence Tomography for Gross Examination and Sampling of Fixed Breast Specimens: A Pilot Study