1. Articles from Fabrice Harms

    1-17 of 17
    1. Dynamic full field optical coherence tomography: subcellular metabolic contrast revealed in tissues by interferometric signals temporal analysis

      Dynamic full field optical coherence tomography: subcellular metabolic contrast revealed in tissues by interferometric signals temporal analysis

      We developed a new endogenous approach to reveal subcellular metabolic contrast in fresh ex vivo tissues taking advantage of the time dependence of the full field optical coherence tomography interferometric signals. This method reveals signals linked with local activity of the endogenous scattering elements which can reveal cells where other OCT-based techniques fail or need exogenous contrast agents. We benefit from the micrometric transverse resolution of full field OCT to image intracellular features. We used this time dependence to identify different dynamics at the millisecond scale on a wide range of organs in normal or pathological conditions.

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    2. High-resolution handheld rigid endomicroscope based on full-field optical coherence tomography

      High-resolution handheld rigid endomicroscope based on full-field optical coherence tomography

      Full-field optical coherence tomography (FF-OCT) is a powerful tool for nondestructive assessment of biological tissue, i.e., for the structural examination of tissue in depth at a cellular resolution. Mostly known as a microscopy device for ex vivo analysis, FF-OCT has also been adapted to endoscopy setups since it shows good potential for in situ cancer diagnosis and biopsy guidance. Nevertheless, all the attempts to perform endoscopic FF-OCT imaging did not go beyond lab setups. We describe here, to the best of our knowledge, the first handheld FF-OCT endoscope based on a tandem interferometry assembly using incoherent illumination. A common-path ...

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    3. Dynamic full field optical coherence tomography: subcellular metabolic contrast revealed in tissues by temporal analysis of interferometric signals

      Dynamic full field optical coherence tomography: subcellular metabolic contrast revealed in tissues by temporal analysis of interferometric signals

      We developed a new endogenous approach to reveal subcellular metabolic contrast in fresh ex vivo tissues taking advantage of the time dependence of the full field optical coherence tomography interferometric signals. This method reveals signals linked with local activity of the endogenous scattering elements which can reveal cells where other imaging techniques fail or need exogenous contrast agents. We benefit from the micrometric transverse resolution of full field OCT to image intracellular features. We used this time dependence to identify different dynamics at the millisecond scale on a wide range of organs in normal or pathological conditions.

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    4. Optical tissue sectioning using full field optical coherence tomography

      Optical tissue sectioning using full field optical coherence tomography

      According to a first aspect, the invention relates to a multimodal optical sectioning microscope (200, 400, 600) for full-field imaging of a volumic and scattering sample comprising: --a full-field OCT system for providing an image of a first section in depth of the sample comprising an illumination sub-system (201, 401, 601) and a full-field imaging interferometer with a detection sub system (208, 408, 608) and an optical conjugation device for optically conjugating the sample and said detection sub system, wherein said optical conjugation device comprises a microscope objective (203, 403, 603), --a supplementary full-field optical sectioning imaging system for providing ...

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    5. Method and device for high resolution full field interference microscopy

      Method and device for high resolution full field interference microscopy

      The invention relates to an incoherent light full field interference microscopy device for the imaging of a volumetric scattering sample (106). The device comprises an interference device (100) between a reference wave (401), produced by reflection of an incident wave by a reflective surface (105) of a reference arm of the interference device, and an object wave (402) produced by backscattering of the incident wave by a slice of the sample, an acquisition device (108) for at least a first interference signal and at least a second interference signal resulting from the interference of the reference and object waves, the ...

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    6. Assessment of Sentinel Node Biopsies With Full-Field Optical Coherence Tomography

      Assessment of Sentinel Node Biopsies With Full-Field Optical Coherence Tomography

      Current techniques for the intraoperative analysis of sentinel lymph nodes during breast cancer surgery present drawbacks such as time and tissue consumption. Full-field optical coherence tomography is a novel noninvasive, high-resolution, fast imaging technique. This study investigated the use of full-field optical coherence tomography as an alternative technique for the intraoperative analysis of sentinel lymph nodes. Seventy-one axillary lymph nodes from 38 patients at Tenon Hospital were imaged minutes after excision with full-field optical coherence tomography in the pathology laboratory, before being handled for histological analysis. A pathologist performed a blind diagnosis (benign/malignant), based on the full-field optical coherence ...

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    7. Full-field optical coherence tomography (FFOCT) for evaluation of endometrial cancer

      Full-field optical coherence tomography (FFOCT) for evaluation of endometrial cancer

      Full-field optical coherence tomography (FFOCT) quickly produces images that resemble conventional pathology images. We examined endometrium in an intra-operative like fashion (more than forty samples). FFOCT-imaged endometrium was recognizable to pathologists and compared favorably with microscopy of the same samples. Additional image enhancements and acquisition techniques were explored and may improve interpretation accuracy. Wider evaluation of images is ongoing, using more pathologist subjects. FFOCT may revolutionize pathology practice in the future by permitting rapid diagnosis and in vivo diagnosis; this is potentially a disruptive new diagnostic technique in pathology.

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    8. Full-Field Optical Coherence Tomography of Human Donor and Pathological Corneas

      Full-Field Optical Coherence Tomography of Human Donor and Pathological Corneas

      Purpose : To evaluate the performance of a full-field optical coherence tomography (FF-OCT) system in the study of human donor and pathological corneas and assess its suitability for use in eye banks. Methods : Our study was carried out using an FF-OCT system developed for non-invasive imaging of tissue structures in depth with ultrahigh resolution (1 µm in all directions). Images were acquired from eight stored human donor corneas (either edematous or after deswelling) and five surgical specimens of corneas with various diseases (bullous keratopathy, lattice corneal dystrophy, stromal scar after keratitis, keratoconus and Fuchs dystrophy). They were compared with standard histology ...

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    9. En-face full-field optical coherence tomography for fast and efficient fingerprints acquisition

      En-face full-field optical coherence tomography for fast and efficient fingerprints acquisition

      Optical coherence tomography (OCT) has been recently proposed by a number of laboratories as a promising tool for fingerprints acquisitions and for fakes discrimination. Indeed OCT being a non-contact, non-destructive optical method that virtually sections the volume of biological tissues that strongly scatter light it appears obvious to use it for fingerprints. Nevertheless most of the OCT setups have to go through the long acquisition of a full 3D image to isolate an “en-face” image suitable for fingerprint analysis. A few “en-face” OCT approaches have been proposed that use either a complex 2D scanning setup and image processing, or a ...

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    10. Full-field OCT: a non-invasive tool for diagnosis and tissue selection

      Full-field OCT: a non-invasive tool for diagnosis and tissue selection

      Full-field optical coherence tomography (FFOCT) enables in-depth, high-resolution tissue imaging at the cellular level. Pathologists using the technology achieve high sensitivity scores, offering possible future improvements in healthcare quality and decreases in costs, especially for cancer care.

      Optical coherence tomography (OCT), on which the technique is based, undertakes interferometric selection of singly backscattered photons using superposition of waves to decipher their characteristics). It is a powerful technique for imaging biological tissues, particularly for eye examination.1 FFOCT, in contrast with most OCT approaches (time domain- or Fourier domain OCT, for example), directly takes 2D ‘ en face' images using megapixel cameras ...

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    11. Imaging of non-tumorous and tumorous human brain tissues with full-field optical coherence tomography

      Imaging of non-tumorous and tumorous human brain tissues with full-field optical coherence tomography

      A prospective study was performed on neurosurgical samples from 18 patients to evaluate the use of full-field optical coherence tomography (FF-OCT) in brain tumor diagnosis. FF-OCT captures en face slices of tissue samples at 1 μm resolution in 3D to a penetration depth of around 200 μm. A 1 cm 2 specimen is scanned at a single depth and processed in about 5 min. This rapid imaging process is non-invasive and requires neither contrast agent injection nor tissue preparation, which makes it particularly well suited to medical imaging applications. Temporal chronic epileptic parenchyma and brain tumors such as meningiomas, low-grade ...

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    12. Large Field, High Resolution Full-Field Optical Coherence Tomography: A Pre-clinical Study of Human Breast Tissue and Cancer Assessment

      Large Field, High Resolution Full-Field Optical Coherence Tomography: A Pre-clinical Study of Human Breast Tissue and Cancer Assessment

      We present a benchmark pilot study in which high-resolution Full-Field Optical Coherence Tomography (FF-OCT) was used to image human breast tissue and is evaluated to assess its ability to aid the pathologist’s management of intra-operative diagnoses. FF-OCT imaging safety was investigated and agreement between FF-OCT and routinely prepared histopathological images was evaluated. The compact setup used for this study provides 1 mm3 resolution and 200 mm imaging depth, and a 2.25 cm2 specimen is scanned in about 7 minutes. 75 breast specimens were imaged from 22 patients (21 women, 1 man) with a mean age of 58 (range ...

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    13. Imaging of non tumorous and tumorous human brain tissue with full-field optical coherence tomography

      Imaging of non tumorous and tumorous human brain tissue with full-field optical coherence tomography

      A prospective study was performed on neurosurgical samples from 18 patients to evaluate the use of Full-Field Optical Coherence Tomography (FF-OCT) in brain tumor diagnosis. FF-OCT captures en face slices of tissue samples at 1μm resolution in 3D with a typical 200μm imaging depth. A 1cm² specimen is scanned at a single depth and processed in about 5 minutes. This rapid imaging process is non-invasive and requires neither contrast agent injection nor tissue preparation, 30 which makes it particularly well suited to medical imaging applications. Temporal chronic epileptic parenchyma and brain tumors such as meningiomas, lowgrade and high-grade ...

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    14. Optical Tissue Sectioning Using Full Field Optical Coherence Tomography

      Optical Tissue Sectioning Using Full Field Optical Coherence Tomography

      According to a first aspect, the invention relates to a multimodal optical sectioning microscope (200, 400, 600) for full-field imaging of a volumic and scattering sample comprising: - a full-field OCT system for providing an image of a first section in depth of the sample comprising an illumination sub-system (201, 401, 601) and a full-field imaging interferometer with a detection sub system (208, 408, 608) and an optical conjugation device for optically conjugating the sample and said detection sub system, wherein said optical conjugation device comprises a microscope objective (203, 403, 603), - a supplementary full-field optical sectioning imaging system for providing ...

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    15. Full-Field Optical Coherence Tomography System For Imaging An Object

      Full-Field Optical Coherence Tomography System For Imaging An Object

      The present invention relates to a full-field optical coherence tomography system (300) for imaging an object (319), comprising a light source (301), a first interferometric device having means for splitting an input light beam and comprising at least a reflecting surface (307), a second interferometric device having means for splitting the spectrally modulated output beam and comprising at least a reflecting surface, a multichannel acquisition device (321), the system (300) being characterized in that at least one of the interferometric devices includes at least a focusing optical element arranged to focus at least one of the input beams or at ...

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    16. Performance assessment of a pupil tracking system for adaptive optics retinal imaging

      Adaptive Optics (AO) is particularly suitable for correction of aberrations that change over time - a necessity for high resolution imaging of the retina. The rapidly changing aberrations originating from eye movements require wavefront sensors (WFS) with high repetition rates. Our approach is enhan ... [Proc. SPIE 7139, 713911 (2008)] published Tue Dec 30, 2008.

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    17. Adaptive optics loop for en-face optical coherence tomography and laser scanning confocal microscopy

      The capabilities of a novel deformable mirror and wave-front sensor combination to correct aberrations in microscopy are analyzed. The deformable mirror, (Mirao52-D, Imagine Eyes) is incorporated with a Shack-Hartmann sensor (HASO, Imagine Optic) within a complex imaging system able to produce simultaneous en-face Optical Coherence Tomography and Laser Scanning Confocal Microscopy images as well as B-scan OCT images. A large angle imaging along one of the scanning directions is demonstrated using the AO loop to correct for the interface optics aberration. The image is split into three panels, and each panel is imaged using its own set of corrections. The ...

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    1-17 of 17
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    1. (17 articles) Fabrice Harms
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    Full-Field Optical Coherence Tomography System For Imaging An Object Optical Tissue Sectioning Using Full Field Optical Coherence Tomography Imaging of non tumorous and tumorous human brain tissue with full-field optical coherence tomography Large Field, High Resolution Full-Field Optical Coherence Tomography: A Pre-clinical Study of Human Breast Tissue and Cancer Assessment Imaging of non-tumorous and tumorous human brain tissues with full-field optical coherence tomography Full-field OCT: a non-invasive tool for diagnosis and tissue selection En-face full-field optical coherence tomography for fast and efficient fingerprints acquisition Full-Field Optical Coherence Tomography of Human Donor and Pathological Corneas Optoretinography: optical measurements of human cone and rod photoreceptor responses to light Nodular skin lesions: correlation of reflectance confocal microscopy (RCM) and optical coherence tomography (OCT) features OCT and Ophthalmology in the Age of Artificial Intelligence, Tuesday Oct 8, 2019 1:00P EDT Compensation for the Influence of Fluctuations in the Distance to the Object During Noncontact Probing in Spectral Domain Optical Coherence Tomography