1. Articles from Irina V. Larina

    1-24 of 57 1 2 3 »
    1. Mouse embryo phenotyping with optical coherence tomography

      Mouse embryo phenotyping with optical coherence tomography

      With the explosion of gene editing tools in recent years, there has been a much greater demand for mouse embryo phenotyping, and traditional methods such as histology and histochemistry experienced a methodological renaissance as they became the principal tools for phenotyping. However, it is important to explore alternative phenotyping options to maximize time and resources and implement volumetric structural analysis for enhanced investigation of phenotypes. Cardiovascular phenotyping, in particular, is important to perform in vivo due to the dramatic structural and functional changes that occur in heart development over relatively short periods of time. Optical coherence tomography (OCT) is one ...

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    2. Following the Beat: Imaging the Valveless Pumping Function in the Early Embryonic Heart

      Following the Beat: Imaging the Valveless Pumping Function in the Early Embryonic Heart

      In vertebrates, the coordinated beat of the early heart tube drives cardiogenesis and supports embryonic growth. How the heart pumps at this valveless stage marks a fascinating problem that is of vital significance for understanding cardiac development and defects. The developing heart achieves its function at the same time as continuous and dramatic morphological changes, which in turn modify its pumping dynamics. The beauty of this muti-time-scale process also highlights its complexity that requires interdisciplinary approaches to study. High-resolution optical imaging, particularly fast, four-dimensional (4D) imaging, plays a critical role in revealing the process of pumping, instructing numerical modeling, and ...

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    3. Dynamic volumetric imaging and cilia beat mapping in the mouse male reproductive tract with optical coherence tomography

      Dynamic volumetric imaging and cilia beat mapping in the mouse male reproductive tract with optical coherence tomography

      Spermatozoa transport within the male reproductive tract is a highly dynamic and biologically important reproductive event. However, due to the lack of live volumetric imaging technologies and quantitative measurements, there is little information on the dynamic aspect and regulation of this process. Here, we presented ex vivo dynamic volumetric imaging of the mouse testis, efferent duct, epididymis, and vas deferens at a micro-scale spatial resolution with optical coherence tomography (OCT). Micro computed tomography imaging is presented as a reference for the proposed OCT imaging. Application of functional OCT analysis allowed for 3D mapping of the cilia beat frequency in the ...

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    4. Ultra-fast dynamic line-field optical coherence elastography

      Ultra-fast dynamic line-field optical coherence elastography

      Abstract In this work, we present an ultra-fast line-field optical coherence elastography system (LF-OCE) with an 11.5 MHz equivalent A-line rate. The system was composed of a line-field spectral domain optical coherence tomography system based on a supercontinuum light source, Michelson-type interferometer, and a high-speed 2D spectrometer. The system performed ultra-fast imaging of elastic waves in tissue-mimicking phantoms of various elasticities.

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    5. In vivo dynamic 3D imaging of oocytes and embryos in the mouse oviduct

      In vivo dynamic 3D imaging of oocytes and embryos in the mouse oviduct

      Developmental biologists have always relied on imaging to shed light on dynamic cellular events. However, processes such as mammalian fertilization and embryogenesis are generally inaccessible for direct imaging. In consequence, how the oviduct (fallopian tube) facilitates the transport of gametes and preimplantation embryos continues to be unanswered. Here we present a combination of intravital window and optical coherence tomography for dynamic, volumetric, in vivo imaging of oocytes and embryos as they are transported through the mouse oviduct. We observed location-dependent circling, oscillating, and long-distance bi-directional movements of oocytes and embryos that suggest regulatory mechanisms driving transport and question established views ...

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    6. Live mechanistic assessment of localized cardiac pumping in mammalian tubular embryonic heart

      Live mechanistic assessment of localized cardiac pumping in mammalian tubular embryonic heart

      Significance: Understanding how the valveless embryonic heart pumps blood is essential to elucidate biomechanical cues regulating cardiogenesis, which is important for the advancement of congenital heart defects research. However, methods capable of embryonic cardiac pumping analysis remain limited, and assessing this highly dynamic process in mammalian embryos is challenging. New approaches are critically needed to address this hurdle. Aim: We report an imaging-based approach for functional assessment of localized pumping dynamics in the early tubular embryonic mouse heart. Approach: Four-dimensional optical coherence tomography was used to obtain structural and Doppler hemodynamic imaging of the beating heart in live mouse embryos ...

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    7. Dynamic Imaging of Mouse Embryos and Cardiac Development in Static Culture

      Dynamic Imaging of Mouse Embryos and Cardiac Development in Static Culture

      Dynamic imaging is a powerful approach to assess the function of a developing organ system. The heart is a dynamic organ that undergoes quick morphological and mechanical changes through early embryonic development. Defining the embyonic mouse heart's normal function is important for our own understanding of human heart development and will inform us on treatments and prevention of congenital heart defects (CHD). Traditional methods such as ultrasound or fluorescence-based microscopy are suitable for live dynamic imaging, are excellent to visualize structure and connect gene expression to phenotypes, but can be of low quality in resolving fine features and lack ...

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    8. Postdoctoral or Research Scientist Position in Biomedical Optics at Baylor College of Medicine

      Postdoctoral or Research Scientist Position in Biomedical Optics at Baylor College of Medicine

      A postdoctoral or research scientist position is available in Larina Lab in the Department of Molecular Physiology and Biophysics at the Baylor College of Medicine, Houston, TX to develop functional optical imaging methods toward investigation of developmental mechanisms. We are a dynamic highly collaborative team of optical engineers and biologists, investigating biomechanical aspects of biological processes in mouse models of human disorders. Our studies bring together optical system design, OCT, confocal/multiphoton microscopy, optogenetics, technology development in functional optical imaging and data processing (4D angiography, cilia beat mapping, cardiodynamic analysis, elastography), vital fluorescent reporters, genetic mouse models of human disorders ...

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    9. Postdoctoral and Research Scientist Positions in Biomedical Imaging at Baylor College of Medicine

      Postdoctoral and Research Scientist Positions in Biomedical Imaging at Baylor College of Medicine

      Postdoctoral and research scientist positions are available in Larina Lab at the Baylor College of Medicine in Houston, Texas to develop functional optical imaging methods toward investigation of reproductive and developmental mechanisms. We are a dynamic highly collaborative team of optical engineers and biologists, investigating biomechanical aspects of biological processes in mouse models of human disorders. Our studies bring together optical system design, OCT, confocal/multiphoton microscopy, optogenetics, technology development in functional optical imaging and data processing (4D angiography, cilia beat mapping, cardiodynamic analysis, elastography), vital fluorescent reporters, genetic mouse models of human disorders, developmental and reproductive biology questions. The ...

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    10. In Vivo Imaging of the Mouse Reproductive Organs, Embryo Transfer, and Oviduct Cilia Dynamics Using Optical Coherence Tomography

      In Vivo Imaging of the Mouse Reproductive Organs, Embryo Transfer, and Oviduct Cilia Dynamics Using Optical Coherence Tomography

      The oviduct (or fallopian tube) serves as the site where a number of major reproductive events occur for the start of a new life in mammals. Understanding the oviduct physiology is essential to uncover hidden mechanisms of the human reproduction and its disorders, yet the current analysis of the oviduct that is largely limited to in vitro imaging is a significant technical hurdle. To overcome this barrier, we have recently developed in vivo approaches based on optical coherence tomography for structural and functional imaging of the mouse oviduct. In this chapter, we describe the details of such live imaging methods ...

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    11. In vivo three-dimensional tracking of sperm behaviors in the mouse oviduct

      In vivo three-dimensional tracking of sperm behaviors in the mouse oviduct

      Mammalian sperm evolutionarily acquired complex mechanisms regulating their behaviors, which are thought to be critical in navigating through the female reproductive tract toward fertilization. However, all current knowledge of this process is largely extrapolated from in vitro and ex vivo studies, because in vivo analysis of sperm in their native fertilization environment has not been possible. Here we report a functional optical coherence tomography approach that allows, for the first time, in vivo three-dimensional (3D) tracking of sperm behaviors in the mouse oviduct. Motile sperm are identified with their intrinsic dynamic characteristics. Sperm trajectories are reconstructed in 3D with a ...

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    12. Functional optical coherence tomography for live dynamic analysis of mouse embryonic cardiogenesis

      Functional optical coherence tomography for live dynamic analysis of mouse embryonic cardiogenesis

      Blood flow, heart contraction, and tissue stiffness are important regulators of cardiac morphogenesis and function during embryonic development. Defining how these factors are integrated is critically important to advance prevention, diagnostics, and treatment of congenital heart defects. Mammalian embryonic development is taking place deep within the female body, which makes cardiodynamic imaging and analysis during early developmental stages in humans inaccessible. With thousands of mutant lines available and well-established genetic manipulation tools, mouse is a great model to understand how biomechanical factors are integrated with molecular pathways to regulate cardiac function and development. Dynamic imaging and quantitative analysis of the ...

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    13. Live dynamic analysis of mouse embryonic cardiogenesis with functional optical coherence tomography

      Live dynamic analysis of mouse embryonic cardiogenesis with functional optical coherence tomography

      Hemodynamic load, contractile forces, and tissue elasticity are regulators of cardiac development and contribute to the mechanical homeostasis of the developing vertebrate heart. Congenital heart disease (CHD) is a prevalent condition in the United States that affects 8 in 1000 live births[1], and has been linked to disrupted cardiac biomechanics[2-4]. Therefore, it is important to understand how these forces integrate and regulate vertebrate cardiac development to inform clinical strategies to treat CHD early on by reintroducing proper mechanical load or modulating downstream factors that rely on mechanical signalling. Toward investigation of biomechanical regulation of mammalian cardiovascular dynamics and ...

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    14. Biomechanical assessment of myocardial infarction using optical coherence elastography

      Biomechanical assessment of myocardial infarction using optical coherence elastography

      Myocardial infarction (MI) leads to cardiomyocyte loss, impaired cardiac function, and heart failure. Molecular genetic analyses of myocardium in mouse models of ischemic heart disease have provided great insight into the mechanisms of heart regeneration, which is promising for novel therapies after MI. Although biomechanical factors are considered an important aspect in cardiomyocyte proliferation, there are limited methods for mechanical assessment of the heart in the mouse MI model. This prevents further understanding the role of tissue biomechanics in cardiac regeneration. Here we report optical coherence elastography (OCE) of the mouse heart after MI. Surgical ligation of the left anterior ...

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    15. Prolonged in vivo functional assessment of the mouse oviduct using optical coherence tomography through a dorsal imaging window

      Prolonged in vivo functional assessment of the mouse oviduct using optical coherence tomography through a dorsal imaging window

      The oviduct (or fallopian tube) serves as an environment for gamete transport, fertilization, and preimplantation embryo development in mammals. Although there has been increasing evidence linking infertility with disrupted oviduct function, the specific roles that the oviduct plays in both normal and impaired reproductive processes remain unclear. The mouse is an important mammalian model to study human reproduction. However, most of the current analyses of the mouse oviduct rely on static histology or 2D visualization, and are unable to provide dynamic and volumetric characterization of this organ. The lack of imaging access prevents longitudinal live analysis of the oviduct and ...

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    16. Comparison and combination of rotational imaging optical coherence tomography and selective plane illumination microscopy for embryonic study

      Comparison and combination of rotational imaging optical coherence tomography and selective plane illumination microscopy for embryonic study

      Several optical imaging techniques have been applied for high-resolution embryonic imaging using different contrast mechanisms, each with their own benefits and limitations. In this study, we imaged the same E9.5 mouse embryo with rotational imaging optical coherence tomography (RI-OCT) and selective plane illumination microscopy (SPIM). RI-OCT overcomes optical penetration limits of traditional OCT imaging that prohibit full-body imaging of mouse embryos at later stages by imaging the samples from multiple angles. SPIM enables high-resolution, 3D imaging with less phototoxicity and photobleaching than laser scanning confocal microscopy (LSCM) by illuminating the sample with a focused sheet of light. Side by ...

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    17. Speckle variance optical coherence tomography of blood flow in the beating mouse embryonic heart

      Speckle variance optical coherence tomography of blood flow in the beating mouse embryonic heart

      Efficient separation of blood and cardiac wall in the beating embryonic heart is essential and critical for experiment-based computational modelling and analysis of early-stage cardiac biomechanics. Although speckle variance optical coherence tomography (SV-OCT) relying on calculation of intensity variance over consecutively acquired frames is a powerful approach for segmentation of fluid flow from static tissue, application of this method in the beating embryonic heart remains challenging because moving structures generate SV signal indistinguishable from the blood. Here, we demonstrate a modified four-dimensional SV-OCT approach that effectively separates the blood flow from the dynamic heart wall in the beating mouse embryonic ...

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    18. A dual-modality optical coherence tomography and selective plane illumination microscopy system for mouse embryonic imaging

      A dual-modality optical coherence tomography and selective plane illumination microscopy system for mouse embryonic imaging

      Both optical coherence tomography (OCT) and selective plane illumination microscopy (SPIM) are frequently used in mouse embryonic research for high-resolution three-dimensional imaging. However, each of these imaging methods provide a unique and independent advantage: SPIM provides morpho-functional information through immunofluorescence and OCT provides a method for whole-embryo 3D imaging. In this study, we have combined rotational imaging OCT and SPIM into a single, dual-modality device to image E9.5 mouse embryos. The results demonstrate that the dual-modality setup is able to provide both anatomical and functional information simultaneously for more comprehensive tissue characterization

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    19. Live dynamic analysis of the developing cardiovascular system in mice

      Live dynamic analysis of the developing cardiovascular system in mice

      The study of the developing cardiovascular system in mice is important for understanding human cardiogenesis and congenital heart defects. Our research focuses on imaging early development in the mouse embryo to specifically understand cardiovascular development under the regulation of dynamic factors like contractile force and blood flow using optical coherence tomography (OCT). We have previously developed an OCT based approach that combines static embryo culture and advanced image processing with computational modeling to live-image mouse embryos and obtain 4D (3D+time) cardiodynamic datasets. Here we present live 4D dynamic blood flow imaging of the early embryonic mouse heart in correlation ...

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    20. Dynamic imaging and quantitative analysis of cranial neural tube closure in the mouse embryo using optical coherence tomography

      Dynamic imaging and quantitative analysis of cranial neural tube closure in the mouse embryo using optical coherence tomography

      Neural tube closure is a critical feature of central nervous system morphogenesis during embryonic development. Failure of this process leads to neural tube defects, one of the most common forms of human congenital defects. Although molecular and genetic studies in model organisms have provided insights into the genes and proteins that are required for normal neural tube development, complications associated with live imaging of neural tube closure in mammals limit efficient morphological analyses. Here, we report the use of optical coherence tomography (OCT) for dynamic imaging and quantitative assessment of cranial neural tube closure in live mouse embryos in culture ...

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    21. Live four-dimensional optical coherence tomography reveals embryonic cardiac phenotype in mouse mutant

      Live four-dimensional optical coherence tomography reveals embryonic cardiac phenotype in mouse mutant

      Efficient phenotyping of developmental defects in model organisms is critical for understanding the genetic specification of normal development and congenital abnormalities in humans. We previously reported that optical coherence tomography (OCT) combined with live embryo culture is a valuable tool for mouse embryo imaging and four-dimensional (4-D) cardiodynamic analysis; however, its capability for analysis of mouse mutants with cardiac phenotypes has not been previously explored. Here, we report 4-D (three-dimensional+time) OCT imaging and analysis of the embryonic heart in a Wdr19 mouse mutant, revealing a heart looping defect. Quantitative analysis of cardiac looping revealed a statistically significant difference between ...

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    22. Applicability, usability, and limitations of murine embryonic imaging with optical coherence tomography and optical projection tomography

      Applicability, usability, and limitations of murine embryonic imaging with optical coherence tomography and optical projection tomography

      We present an analysis of imaging murine embryos at various embryonic developmental stages (embryonic day 9.5, 11.5, and 13.5) by optical coherence tomography (OCT) and optical projection tomography (OPT). We demonstrate that while OCT was capable of rapid high-resolution live 3D imaging, its limited penetration depth prevented visualization of deeper structures, particularly in later stage embryos. In contrast, OPT was able to image the whole embryos, but could not be used in vivo because the embryos must be fixed and cleared. Moreover, the fixation process significantly altered the embryo morphology, which was quantified by the volume of ...

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    23. Four-dimensional live imaging of hemodynamics in mammalian embryonic heart with Doppler optical coherence tomography

      Four-dimensional live imaging of hemodynamics in mammalian embryonic heart with Doppler optical coherence tomography

      Four-dimensional live hemodynamic imaging of the mouse embryonic heart at embryonic day 9.0 using Doppler optical coherence tomography, showing directional blood flows in the sinus venosus, primitive atrium, atrioventricular region and vitelline vein. Hemodynamic analysis of the mouse embryonic heart is essential for understanding the functional aspects of early cardiogenesis and advancing the research in congenital heart defects. However, high-resolution imaging of cardiac hemodynamics in mammalian models remains challenging, primarily due to the dynamic nature and deep location of the embryonic heart. Here we report four-dimensional micro-scale imaging of blood flow in the early mouse embryonic heart, enabling time-resolved ...

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    24. Comparison of rotational imaging optical coherence tomography and selective plane illumination microscopy for embryonic study

      Comparison of rotational imaging optical coherence tomography and selective plane illumination microscopy for embryonic study

      The mouse is a common model for studying developmental diseases. Different optical techniques have been developed to investigate mouse embryos, but each has its own set of limitations and restrictions. In this study, we imaged the same E9.5 mouse embryo with rotational imaging Optical Coherence Tomography (RI-OCT) and Selective Plane Illumination Microscopy (SPIM), and compared the two techniques. Results demonstrate that both methods can provide images with micrometer-scale spatial resolution. The RI-OCT technique was developed to increase imaging depth of OCT by performing traditional OCT imaging at multiple sides and co-registering the images. In SPIM, optical sectioning is achieved ...

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    1-24 of 57 1 2 3 »
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