1. Articles from Kate Grieve

    1-13 of 13
    1. Spectral Domain Optical Coherence Tomography in Limbal Stem Cell Deficiency. A Case Control Study

      Spectral Domain Optical Coherence Tomography in Limbal Stem Cell Deficiency. A Case Control Study

      Purpose To evaluate the efficacy of optical coherence tomography as a non-contact method for imaging the ocular surface in limbal stem cell deficiency (LSCD) and normal eyes. Design Retrospective case-control study. Methods: Setting Institutional. Study population 22 eyes with LSCD (study group, 22 patients) and 10 normal eyes (control group, 10 patients). Observation procedures SD-OCT and confocal microscopy in both the limbal and central corneal zones. Main outcome measures Pachymetry data from the central cornea, presence of the palisades of Vogt, limbal crypts and clear transition between the hyporeflective corneal epithelium and the hyperreflective conjunctival epithelium assessed on cross-sections parallel ...

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      Mentions: Kate Grieve
    2. In vivo imaging through the entire thickness of human cornea by full-field optical coherence tomography

      In vivo imaging through the entire thickness of human cornea by full-field optical coherence tomography

      Despite obvious improvements in visualization of the in vivo cornea through the faster imaging speeds and higher axial resolutions, cellular imaging stays unresolvable task for OCT, as en face viewing with a high lateral resolution is required. The latter is possible with FFOCT, a method that relies on a camera, moderate numerical aperture (NA) objectives and an incoherent light source to provide en face images with a micrometer-level resolution. Recently, we for the first time demonstrated the ability of FFOCT to capture images from the in vivo human cornea1. In the current paper we present an extensive study of appearance ...

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    3. In vivo high resolution human corneal imaging using full-field optical coherence tomography

      In vivo high resolution human corneal imaging using full-field optical coherence tomography

      We present the first full-field optical coherence tomography (FFOCT) device capable of in vivo imaging of the human cornea. We obtained images of the epithelial structures, Bowman’s layer, sub-basal nerve plexus (SNP), anterior and posterior stromal keratocytes, stromal nerves, Descemet’s membrane and endothelial cells with visible nuclei. Images were acquired with a high lateral resolution of 1.7 µm and relatively large field-of-view of 1.26 mm x 1.26 mm – a combination, which, to the best of our knowledge, has not been possible with other in vivo human eye imaging methods. The latter together with a contactless ...

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    4. Cell Motility as Contrast Agent in Retinal Explant Imaging With Full-Field Optical Coherence Tomography

      Cell Motility as Contrast Agent in Retinal Explant Imaging With Full-Field Optical Coherence Tomography

      Purpose : To use cell motility as a contrast agent in retinal explants. Methods : Macaque and mouse retinal explants were imaged with high resolution full field optical coherence tomography (FFOCT) and dynamic FFOCT, coupled with fluorescence imaging. Results : Static and dynamic FFOCT create complementary contrast from different structures within a cell. When imaging in vitro samples, static FFOCT detects steep refractive index gradients to reveal stationary structures including fibers, vessels, collagen, and cell contours, while dynamic FFOCT emphasizes metabolic activity of moving structures that are mainly intracellular, thus creating or enhancing contrast in cells that were previously hidden in noise. Dynamic ...

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    5. Full-field OCT technique for high speed event-based optical flow and particle tracking

      Full-field OCT technique for high speed event-based optical flow and particle tracking

      This article introduces a method to extract the speed and density of microparticles in real time at several kHz using an asynchronous event-based camera mounted on a full-field optical coherence tomography (FF-OCT) setup. These cameras detect significant amplitude changes, allowing scene-driven acquisitions. They are composed of an array of autonomously operating pixels. Events are triggered when an illuminance change at the pixel level is significant at 1 μs time precision. The event-driven FF-OCT algorithm relies on a time-based optical flow computation to operate directly on incoming events and updates the estimation of velocity, direction and density while reducing both computation ...

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      Mentions: Kate Grieve
    6. En face coherence microscopy [Invited]

      En face coherence microscopy [Invited]

      En face coherence microscopy or flying spot or full field optical coherence tomography or microscopy (FF-OCT/FF-OCM) belongs to the OCT family because the sectioning ability is mostly linked to the source coherence length. In this article we will focus our attention on the advantages and the drawbacks of the following approaches: en face versus B scan tomography in terms of resolution, coherent versus incoherent illumination and influence of aberrations, and scanning versus full field imaging. We then show some examples to illustrate the diverse applications of en face coherent microscopy and show that endogenous or exogenous contrasts can add ...

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    7. Appearance of the Retina With Full-Field Optical Coherence Tomography

      Appearance of the Retina With Full-Field Optical Coherence Tomography

      Purpose : To interpret full-field optical coherence tomography (FFOCT) images of ex vivo retina. Methods : Flatmounted retinas of human, primate, pig, sheep, rat, and mouse were imaged using FFOCT. To identify retinal ganglion and amacrine cells, fixed samples immunolabeled against Tuj1 and Brn3a or live samples labeled in vitro with green fluorescent protein (GFP) were analyzed by combining FFOCT, fluorescence confocal microscopy (FCM), and fluorescence-FFOCT. To investigate postmortem tissue changes, time series were acquired over 48 hours and on fresh versus fixed tissue. Results : With FFOCT, cell types and features such as nerve fiber bundles and RGC somas were resolved without ...

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    8. Assessment of Sentinel Node Biopsies With Full-Field Optical Coherence Tomography

      Assessment of Sentinel Node Biopsies With Full-Field Optical Coherence Tomography

      Current techniques for the intraoperative analysis of sentinel lymph nodes during breast cancer surgery present drawbacks such as time and tissue consumption. Full-field optical coherence tomography is a novel noninvasive, high-resolution, fast imaging technique. This study investigated the use of full-field optical coherence tomography as an alternative technique for the intraoperative analysis of sentinel lymph nodes. Seventy-one axillary lymph nodes from 38 patients at Tenon Hospital were imaged minutes after excision with full-field optical coherence tomography in the pathology laboratory, before being handled for histological analysis. A pathologist performed a blind diagnosis (benign/malignant), based on the full-field optical coherence ...

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    9. A feasibility study of full-field optical coherence tomography for rapid evaluation of EUS-guided microbiopsy specimens

      A feasibility study of full-field optical coherence tomography for rapid evaluation of EUS-guided microbiopsy specimens

      Background Rapid on-site evaluation of cytologic specimens is a way of determining the adequacy of fine-needle aspiration (FNA). However, alternatives may be useful when the presence of a cytotechnologist and/or pathologist is not possible. Objective To evaluate the feasibility of using full-field optical coherence tomography (FFOCT) for FNA specimen quality assessment. Design FFOCT images were acquired on gastric, pancreatic, pelvic, and lymph-node formalin-fixed FNA specimens and were compared with histology of the same samples. Setting Pathology suite in a hospital. Patients Fourteen patients undergoing gastric, pancreatic, pelvic, or lymph-node EUS-guided FNA biopsy. Interventions FFOCT imaging on formalin-fixed samples before ...

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    10. Full-Field Optical Coherence Tomography of Human Donor and Pathological Corneas

      Full-Field Optical Coherence Tomography of Human Donor and Pathological Corneas

      Purpose : To evaluate the performance of a full-field optical coherence tomography (FF-OCT) system in the study of human donor and pathological corneas and assess its suitability for use in eye banks. Methods : Our study was carried out using an FF-OCT system developed for non-invasive imaging of tissue structures in depth with ultrahigh resolution (1 µm in all directions). Images were acquired from eight stored human donor corneas (either edematous or after deswelling) and five surgical specimens of corneas with various diseases (bullous keratopathy, lattice corneal dystrophy, stromal scar after keratitis, keratoconus and Fuchs dystrophy). They were compared with standard histology ...

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    11. Imaging of non-tumorous and tumorous human brain tissues with full-field optical coherence tomography

      Imaging of non-tumorous and tumorous human brain tissues with full-field optical coherence tomography

      A prospective study was performed on neurosurgical samples from 18 patients to evaluate the use of full-field optical coherence tomography (FF-OCT) in brain tumor diagnosis. FF-OCT captures en face slices of tissue samples at 1 μm resolution in 3D to a penetration depth of around 200 μm. A 1 cm 2 specimen is scanned at a single depth and processed in about 5 min. This rapid imaging process is non-invasive and requires neither contrast agent injection nor tissue preparation, which makes it particularly well suited to medical imaging applications. Temporal chronic epileptic parenchyma and brain tumors such as meningiomas, low-grade ...

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    12. Large Field, High Resolution Full-Field Optical Coherence Tomography: A Pre-clinical Study of Human Breast Tissue and Cancer Assessment

      Large Field, High Resolution Full-Field Optical Coherence Tomography: A Pre-clinical Study of Human Breast Tissue and Cancer Assessment

      We present a benchmark pilot study in which high-resolution Full-Field Optical Coherence Tomography (FF-OCT) was used to image human breast tissue and is evaluated to assess its ability to aid the pathologist’s management of intra-operative diagnoses. FF-OCT imaging safety was investigated and agreement between FF-OCT and routinely prepared histopathological images was evaluated. The compact setup used for this study provides 1 mm3 resolution and 200 mm imaging depth, and a 2.25 cm2 specimen is scanned in about 7 minutes. 75 breast specimens were imaged from 22 patients (21 women, 1 man) with a mean age of 58 (range ...

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    13. Imaging of non tumorous and tumorous human brain tissue with full-field optical coherence tomography

      Imaging of non tumorous and tumorous human brain tissue with full-field optical coherence tomography

      A prospective study was performed on neurosurgical samples from 18 patients to evaluate the use of Full-Field Optical Coherence Tomography (FF-OCT) in brain tumor diagnosis. FF-OCT captures en face slices of tissue samples at 1μm resolution in 3D with a typical 200μm imaging depth. A 1cm² specimen is scanned at a single depth and processed in about 5 minutes. This rapid imaging process is non-invasive and requires neither contrast agent injection nor tissue preparation, 30 which makes it particularly well suited to medical imaging applications. Temporal chronic epileptic parenchyma and brain tumors such as meningiomas, lowgrade and high-grade ...

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    1-13 of 13
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    1. (13 articles) Kate Grieve
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    Imaging of non tumorous and tumorous human brain tissue with full-field optical coherence tomography Large Field, High Resolution Full-Field Optical Coherence Tomography: A Pre-clinical Study of Human Breast Tissue and Cancer Assessment Imaging of non-tumorous and tumorous human brain tissues with full-field optical coherence tomography Full-Field Optical Coherence Tomography of Human Donor and Pathological Corneas A feasibility study of full-field optical coherence tomography for rapid evaluation of EUS-guided microbiopsy specimens Assessment of Sentinel Node Biopsies With Full-Field Optical Coherence Tomography Appearance of the Retina With Full-Field Optical Coherence Tomography En face coherence microscopy [Invited] Full-field OCT technique for high speed event-based optical flow and particle tracking In vivo high resolution human corneal imaging using full-field optical coherence tomography OCT-A Pinpoints Irregularities in Best Disease Spectrally encoded coherence tomography and reflectometry: Simultaneous en face and cross‐sectional imaging at 2 gigapixels per second