1. Articles from Christine A. Curcio

    1-25 of 25
    1. Correlation of Optical Coherence Tomography Angiography of Type 3 Macular Neovascularization With Corresponding Histology

      Correlation of Optical Coherence Tomography Angiography of Type 3 Macular Neovascularization With Corresponding Histology

      Importance By validating optical coherence tomography angiography (OCTA) in the analysis of type 3 macular neovascularization secondary to age-related macular degeneration, the overall value of clinical OCTA for disease observation, diagnosis, and staging is increased. Objective To assess the association of in vivo OCTA of type 3 macular neovascularization secondary to age-related macular degeneration with corresponding ex vivo histology. Design, Setting, and Participants This study included clinical imaging, laboratory microscopy, and eye-tracked clinicopathologic correlation of a single case from a community-based practice evaluated at a university-based research laboratory from 2014 to 2019. Exposures Infrared reflectance and eye-tracked spectral-domain OCTA clinical ...

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    2. Mitochondrial distribution in the outer plexiform layer of human retina - Does it correlate with reflectivity in OCT?

      Mitochondrial distribution in the outer plexiform layer of human retina - Does it correlate with reflectivity in OCT?

      Mitochondria are organelles essential for cellular metabolism that also contribute reflectivity to optical coherence tomography (OCT) via Mie scattering. To find reflectivity sources, we assessed mitochondrial distribution of outer plexiform layer (OPL) using comprehensive volume electron microscopy. Parafoveal retina of a 21-year-old male donor was subject to serial block-face scanning electron microscopy in 5×5×50 nm³ voxels. A convolutional neural network (U-Net) was used to train and generate a deep learning model from manually annotated mitochondria ground truth (Dragonfly 2020.1, Object Research Systems). Using this model, mitochondrial volume and occupancy were calculated as a function of retinal depth ...

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    3. Hyperreflective Foci, Optical Coherence Tomography Progression Indicators in Age-Related Macular Degeneration, Include Transdifferentiated Retinal Pigment Epithelium

      Hyperreflective Foci, Optical Coherence Tomography Progression Indicators in Age-Related Macular Degeneration, Include Transdifferentiated Retinal Pigment Epithelium

      Purpose: By optical coherence tomography (OCT) imaging, hyperreflective foci (HRF) indicate progression risk for advanced age-related macular degeneration (AMD) and are in part attributable to ectopic retinal pigment epithelium (RPE). We hypothesized that ectopic RPE are molecularly distinct from in-layer cells and that their cross-retinal course follows Müller glia. Methods: In clinical OCT (61 eyes, 44 patients with AMD, 79.4 ± 7.7 years; 29 female; follow-up = 4.7 ± 0.9 years), one HRF type, RPE plume (n = 129 in 4 morphologies), was reviewed. Twenty eyes of 20 donors characterized by ex vivo OCT were analyzed by histology (normal ...

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    4. MULTIMODAL IMAGING AND HISTOLOGIC EVIDENCE OF A NOVEL OPTICAL COHERENCE TOMOGRAPHY TERMINOLOGY

      MULTIMODAL IMAGING AND HISTOLOGIC EVIDENCE OF A NOVEL OPTICAL COHERENCE TOMOGRAPHY TERMINOLOGY

      Purpose: To clarify the histologic basis of bacillary layer detachment (BALAD) through a review of current literature and an analysis of retinal imaging. Methods: We reviewed the literature for previous reports of BALAD. An analysis of retinal images was performed to support anatomic conclusions. Results: A total of 164 unique patients with BALAD on optical coherence tomography (OCT) were identified from the published literature. Twenty-two underlying etiologies, all associated with subretinal exudation, were identified. Forty-one different OCT terminologies were found. The defining OCT feature of BALAD was a split at the level of the photoreceptor inner segment myoid creating a ...

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    5. Atlas of Human Retinal Pigment Epithelium Organelles Significant for Clinical Imaging

      Atlas of Human Retinal Pigment Epithelium Organelles Significant for Clinical Imaging

      Purpose : To quantify organelles impacting imaging in the cell body and intact apical processes of human retinal pigment epithelium (RPE), including melanosomes, lipofuscin–melanolipofuscin (LM), mitochondria, and nuclei. Methods : A normal perifovea of a 21-year-old white male was preserved after rapid organ recovery. An aligned image stack was generated using serial block-face scanning electron microscopy and was annotated by expert readers (TrakEM, ImageJ). Acquired measures included cell body and nuclear volume ( n = 17); organelle count in apical processes ( n = 17) and cell bodies ( n = 8); distance of cell body organelles along a normalized apical–basal axis ( n = 8); and dimensions ...

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    6. Linear and planar reflection artifacts on swept-source and spectral-domain optical coherence tomography due to hyperreflective crystalline deposits

      Linear and planar reflection artifacts on swept-source and spectral-domain optical coherence tomography due to hyperreflective crystalline deposits

      Purpose To describe novel spectral-domain (SD) and swept-source (SS) optical coherence tomography (OCT) linear and planar reflection artifacts produced by hyperreflective crystalline deposits (HCD). Methods Imaging from 10 eyes with HCD producing linear and planar artifacts on OCT was retrospectively analyzed. All eyes had SD-OCT (Spectralis HRA + OCT, Heidelberg Engineering, Germany) and SS-OCT angiography (PLEX Elite 9000, Carl Zeiss Meditec, Inc., Dublin, CA) acquired on the same day. The horizontal extent of planar artifacts and the corresponding HCD on B-scans was measured using a digital caliper. Artifact features from HCD in eyes with non-neovascular age-related macular degeneration (AMD) were analyzed ...

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    7. The role of Müller cells in tractional macular disorders: an optical coherence tomography study and physical model of mechanical force transmission

      The role of Müller cells in tractional macular disorders: an optical coherence tomography study and physical model of mechanical force transmission

      Background To explore the role of foveal and parafoveal Müller cells in the morphology and pathophysiology of tractional macular disorders with a mathematical model of mechanical force transmission. Methods In this retrospective observational study, spectral-domain optical coherence tomography images of tractional lamellar macular holes and patients with myopic foveoschisis were reviewed and analysed with a mathematical model of force transmission. Parafoveal z-shaped Müller cells were modelled as a structure composed of three rigid rods, named R1, R2 and R3. The angle formed between the rods was referred to as θ . R1, R2 and R3 lengths as well as the ...

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    8. Retinal pathology on optical coherence tomography among eyes of older adults judged normal by color fundus photography

      Retinal pathology on optical coherence tomography among eyes of older adults judged normal by color fundus photography

      Purpose Optical coherence tomography (OCT) has revealed many details of retinal disease that were not available with older imaging technologies. In eyes of adults >60 years old with normal maculas as determined by color fundus photography (CFP) and a validated grading system, we screened for pathology using OCT. We also tested visual functions to assess potential impact of the observed pathologies on patients. Design cross-sectional study Participants This study recruited persons from primary ophthalmology care clinics. METHODS: CFP were assessed by the 9-step AREDS scale. OCT macular volumes of participants at step 1 on the AREDS scale, considered normal, were ...

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    9. The Evolution of the Plateau, an Optical Coherence Tomography Signature Seen in Geographic Atrophy

      The Evolution of the Plateau, an Optical Coherence Tomography Signature Seen in Geographic Atrophy

      Purpose : Histologic details of progression routes to geographic atrophy (GA) in AMD are becoming available through optical coherence tomography (OCT). We studied the origins and evolution of an OCT signature called plateau in eyes with GA and suggested a histologic correlate. Methods : Serial eye-tracked OCT scans and multimodal imaging were acquired from eight eyes of seven patients with GA and plateau signatures over a mean follow-up of 7.7 years (range, 3.7–11.6). The histology of unrelated donor eyes with AMD was reviewed. Results : Drusenoid pigment epithelial detachment (PED) on OCT imaging progressed into wide-based mound-like signatures with ...

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    10. Exploring Photoreceptor Reflectivity Through Multimodal Imaging Of Outer Retinal Tubulation In Advanced AGE-RELATED Macular Degeneration

      Exploring Photoreceptor Reflectivity Through Multimodal Imaging Of Outer Retinal Tubulation In Advanced AGE-RELATED Macular Degeneration

      Purpose: To investigate the microscopic structure of outer retinal tubulation (ORT) and optical properties of cone photoreceptors in vivo, we studied ORT appearance by multimodal imaging, including spectral domain optical coherence tomography (SD-OCT) and adaptive optics scanning laser ophthalmoscopy. Methods: Four eyes of four subjects with advanced age-related macular degeneration underwent color fundus photography, infrared reflectance imaging, SD-OCT, and adaptive optics scanning laser ophthalmoscopy with a high-resolution research instrument. Outer retinal tubulation was identified in closely spaced (11 μ m) SD-OCT volume scans. Results: Outer retinal tubulation in cross-sectional and en face SD-OCT was a hyporeflective area representing a lumen surrounded ...

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    11. Histologic and Optical Coherence Tomographic Correlates in Drusenoid Pigment Epithelium Detachment in Age-Related Macular Degeneration

      Histologic and Optical Coherence Tomographic Correlates in Drusenoid Pigment Epithelium Detachment in Age-Related Macular Degeneration

      Purpose Drusenoid pigment epithelium detachment (DPED) is a known precursor to geographic atrophy in age-related macular degeneration (AMD). We sought histologic correlates for spectral-domain (SD) optical coherence tomography (OCT) signatures in DPED and determined the frequency and origin of these OCT signatures in a clinical cohort of DPED eyes. Design Laboratory imaging and histologic comparison, and retrospective, observational cohort study. Participants Four donor eyes with histopathologic diagnosis of AMD (2 with nonneovascular DPED and 2 with neovascular pigment epithelium detachment [PED]) and 49 eyes of 33 clinic patients with nonneovascular DPED more than 2 mm in diameter. Methods Donor eyes ...

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    12. Evaluation of Segmentation of the Superficial and Deep Vascular Layers of the Retina By Optical Coherence Tomography Angiography Instruments in Normal Eyes

      Evaluation of Segmentation of the Superficial and Deep Vascular Layers of the Retina By Optical Coherence Tomography Angiography Instruments in Normal Eyes

      Importance Correct attribution of vascular features in optical coherence tomography (OCT) angiography depends on accurate segmentation of retinal layers. Objective To evaluate the segmentation of retinal layers among 3 OCT angiography instruments in the central macula, an area where the superficial and deep vascular plexuses terminate. Design, Setting, and Participants A retrospective review of a representative OCT angiogram from 1 patient and an evaluation of the vascular pattern in an autopsied eye were conducted at a community retina practice at a university laboratory. A set of 3 × 3-mm scans centered on the fovea using the Cirrus 5000, RTVue XR Avanti ...

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    13. Quantitative Analysis of Outer Retinal Tubulation in Age-Related Macular Degeneration From Spectral-Domain Optical Coherence Tomography and Histology

      Quantitative Analysis of Outer Retinal Tubulation in Age-Related Macular Degeneration From Spectral-Domain Optical Coherence Tomography and Histology

      Purpose : To assess outer retinal tubulation (ORT) morphology from spectral-domain optical coherence tomography (SD-OCT) volumes and donor eye histology, analyze ORT reflectivity, and estimate the number of cones surviving in ORT. Methods : In SD-OCT volumes from nine patients with advanced AMD, ORT was analyzed en face and in B-scans. The hyperreflective ORT border in cross-section was delineated and surface area calculated. Reflectivity was compared between ORT types (Closed, Open, Forming, and Branching). A flatmount retina from a donor with neovascular AMD was labeled to visualize the external limiting membrane that delimits ORT and allow measurements of cross-sectional cone area, center-to-center ...

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    14. RefMoB, a Reflectivity Feature Model-Based Automated Method for Measuring Four Outer Retinal Hyperreflective Bands in Optical Coherence Tomography

      RefMoB, a Reflectivity Feature Model-Based Automated Method for Measuring Four Outer Retinal Hyperreflective Bands in Optical Coherence Tomography

      Purpose. : To validate a model-driven method (RefMoB) of automatically describing the four outer retinal hyperreflective bands revealed by spectral-domain optical coherence tomography (SDOCT), for comparison with histology of normal macula; to report thickness and position of bands, particularly band 2 (ellipsoid zone [EZ], commonly called IS/OS). Methods. : Foveal and superior perifoveal scans of seven SDOCT volumes of five individuals aged 28 to 69 years with healthy maculas were used (seven eyes for validation, five eyes for measurement). RefMoB determines band thickness and position by a multistage procedure that models reflectivities as a summation of Gaussians. Band thickness and positions ...

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    15. Optical Coherence Tomographic Findings Correspond to Histology

      Optical Coherence Tomographic Findings Correspond to Histology

      Purpose: To compare optical coherence tomography (OCT) and histology of outer retinal tubulation (ORT) secondary to advanced age-related macular degeneration in patients and in postmortem specimens, with particular attention to the basis of the hyperreflective border of ORT. Method: A private referral practice (imaging) and an academic research laboratory (histology) collaborated on two retrospective case series. High-resolution OCT raster scans of 43 eyes (34 patients) manifesting ORT secondary to advanced age-related macular degeneration were compared to high-resolution histologic sections through the fovea and superior perifovea of donor eyes (13 atrophic age-related macular degeneration and 40 neovascular age-related macular degeneration) preserved ...

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    16. Functional optical coherence tomography enables in vivo physiological assessment of retinal rod and cone photoreceptors

      Functional optical coherence tomography enables in vivo physiological assessment of retinal rod and cone photoreceptors

      Transient intrinsic optical signal (IOS) changes have been observed in retinal photoreceptors, suggesting a unique biomarker for eye disease detection. However, clinical deployment of IOS imaging is challenging due to unclear IOS sources and limited signal-to-noise ratios (SNRs). Here, by developing high spatiotemporal resolution optical coherence tomography (OCT) and applying an adaptive algorithm for IOS processing, we were able to record robust IOSs from single-pass measurements. Transient IOSs, which might reflect an early stage of light phototransduction, are consistently observed in the photoreceptor outer segment almost immediately (<4 ms) after retinal stimulation. Comparative studies of dark- and light-adapted retinas have ...

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    17. The Project MACULA retinal pigment epithelium grading system for histology and optical coherence tomography in age-related macular degeneration

      The Project MACULA retinal pigment epithelium grading system for histology and optical coherence tomography in age-related macular degeneration

      Purpose: To seek pathways of retinal pigment epithelium (RPE) fate in age-related macular degeneration via a morphology grading system ; provide nomenclature, visualization targets, and metrics for cl in ical imag in g and model system s. Methods: Donor eyes with geographic atrophy (GA) or choroidal neovascularization (CNV) and one GA eye with previous cl in ical spectral doma in optical coherence tomography (SDOCT) imag in g were processed for histology , photodocumented, and annotated at pre-def in ed locations. RPE cells conta in ed sp in dle-shaped melanosomes, apposed a basal lam in a or basal lam in ar deposit (BLamD ...

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    18. OUTER RETINAL TUBULATION IN ADVANCED AGE-RELATED MACULAR DEGENERATION: Optical Coherence Tomographic Findings Correspond to Histology.

      OUTER RETINAL TUBULATION IN ADVANCED AGE-RELATED MACULAR DEGENERATION: Optical Coherence Tomographic Findings Correspond to Histology.

      Purpose: To compare optical coherence tomography (OCT) and histology of outer retinal tubulation (ORT) secondary to advanced age-related macular degeneration in patients and in postmortem specimens, with particular attention to the basis of the hyperreflective border of ORT. Method: A private referral practice (imaging) and an academic research laboratory (histology) collaborated on two retrospective case series. High-resolution OCT raster scans of 43 eyes (34 patients) manifesting ORT secondary to advanced age-related macular degeneration were compared to high-resolution histologic sections through the fovea and superior perifovea of donor eyes (13 atrophic age-related macular degeneration and 40 neovascular age-related macular degeneration) preserved ...

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    19. Microstructure of subretinal drusenoid deposits revealed by adaptive optics imaging

      Microstructure of subretinal drusenoid deposits revealed by adaptive optics imaging

      Subretinal drusenoid deposits (SDD), a recently recognized lesion associated with progression of age-related macular degeneration, were imaged with adaptive optics scanning laser ophthalmoscopy (AO-SLO) and optical coherence tomography (AO-OCT). AO-SLO revealed a distinct en face structure of stage 3 SDD, showing a hyporeflective annulus surrounded reflective core packed with hyperreflective dots bearing a superficial similarity to the photoreceptors in the unaffected retina. However, AO-OCT suggested that the speckled appearance over the SDD rendered by AO-SLO was the lesion material itself, rather than photoreceptors. AO-OCT assists proper interpretation and understanding of the SDD structure and the lesions’ impact on surrounding photoreceptors ...

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    20. In vivo Optical Coherence Tomography of Light-Driven Melanosome Translocation in Retinal Pigment Epithelium

      In vivo Optical Coherence Tomography of Light-Driven Melanosome Translocation in Retinal Pigment Epithelium

      Optical coherence tomography (OCT) may revolutionize fundamental investigation and clinical management of age-related macular degeneration and other eye diseases. However, quantitative OCT interpretation is hampered due to uncertain sub-cellular correlates of reflectivity in the retinal pigment epithelium (RPE) and photoreceptor. The purpose of this study was twofold: 1) to test OCT correlates in the RPE, and 2) to demonstrate the feasibility of longitudinal OCT monitoring of sub-cellular RPE dynamics. A high resolution OCT was constructed to achieve dynamic imaging of frog eyes, in which light-driven translocation of RPE melanosomes occurred within the RPE cell body and apical processes. Comparative histological ...

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    21. Inner Macular Hyperreflectivity Demonstrated by Optical Coherence Tomography in Niemann-Pick Disease

      Inner Macular Hyperreflectivity Demonstrated by Optical Coherence Tomography in Niemann-Pick Disease

      We describe spectral-domain optical coherence tomography (OCT; Heidelberg Spectralis) imaging of 3 patients with Niemann-Pick disease type B. This expands the range of previously reported anatomical correlates of the ophthalmoscopic macular halo surrounding the “cherry-red spot.”

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    22. Investigation of the hyper-reflective inner/outer segment band in optical coherence tomography of living frog retina

      Investigation of the hyper-reflective inner/outer segment band in optical coherence tomography of living frog retina

      This study is to test anatomic correlates, including connecting cilium (CC) and inner segment (IS) ellipsoid, to the hyper-reflective band visualized by optical coherence tomography (OCT) and commonly attributed to the photoreceptor inner/outer segment (IS/OS) junction. A line-scan OCT (LS-OCT) was constructed to achieve sub-cellular resolution (lateral: ∼ 2  μm; axial: ∼ 4  μm) of excised living frog retinas. An electro-optic phase modulator was employed for rapid and vibration-free phase modulation. Comparison of normalized distance measurements between LS-OCT images and histological images revealed that the dominant source of the signal reported as the IS/OS OCT band actually originates from ...

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    23. ANATOMICAL CORRELATES TO THE BANDS SEEN IN THE OUTER RETINA BY OPTICAL COHERENCE TOMOGRAPHY: Literature Review and Model

      ANATOMICAL CORRELATES TO THE BANDS SEEN IN THE OUTER RETINA BY OPTICAL COHERENCE TOMOGRAPHY: Literature Review and Model
      Purpose: To evaluate the validity of commonly used anatomical designations for the four hyperreflective outer retinal bands seen in current-generation optical coherence tomography, a scale model of outer retinal morphology was created using published information for direct comparison with optical coherence tomography scans. Methods: Articles and books concerning histology of the outer retina from 1900 until 2009 were evaluated, and data were used to create a scale model drawing. Boundaries between outer retinal tissue compartments described by the model were compared with intensity variations of representative spectral-domain optical coherence tomography scans using longitudinal reflectance profiles to determine the region of ...
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    1-25 of 25
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    ANATOMICAL CORRELATES TO THE BANDS SEEN IN THE OUTER RETINA BY OPTICAL COHERENCE TOMOGRAPHY: Literature Review and Model Investigation of the hyper-reflective inner/outer segment band in optical coherence tomography of living frog retina Inner Macular Hyperreflectivity Demonstrated by Optical Coherence Tomography in Niemann-Pick Disease In vivo Optical Coherence Tomography of Light-Driven Melanosome Translocation in Retinal Pigment Epithelium Microstructure of subretinal drusenoid deposits revealed by adaptive optics imaging The Project MACULA retinal pigment epithelium grading system for histology and optical coherence tomography in age-related macular degeneration Functional optical coherence tomography enables in vivo physiological assessment of retinal rod and cone photoreceptors Evaluation of Segmentation of the Superficial and Deep Vascular Layers of the Retina By Optical Coherence Tomography Angiography Instruments in Normal Eyes Commentary on Lavia et al Progress of Optical Coherence Tomography Angiography for Visualizing Human Retinal Vasculature Commentary on Lavia et al: Progress of Optical Coherence Tomography Angiography for Visualizing Human Retinal Vasculature New insights from non-invasive imaging: from prospection of skin photodamages to training with mobile application Short-sighted woman assumes debilitating headaches mean she needs new glasses but discovers she has multiple sclerosis