The feasibility of a full-field optical coherence tomography (FFOCT) system for rapid wide field optical analysis of normal and malignant human ovarian tissue pathologies was demonstrated. Five features were extracted from the normalized image histogram from 56 FFOCT images, based on the differences in the morphology of the normal and malignant tissue samples.
In this study, a full field optical coherence tomography (FFOCT) system was used to analyze and classify normal and malignant human ovarian tissue. 14 ovarian tissue samples (7 normal, 7 malignant) were imaged with the FFOCT system and five features were extracted by analyzing the normalized image histogram from 56 FFOCT images, based on the differences in the morphology of the normal and malignant tissue samples. A generalized linear model (GLM) classifier was trained using 36 images, and sensitivity of 95.3% and specificity of 91.1% was obtained. 20 images were used to test the model, and a sensitivity ...
In this manuscript, the initial feasibility of a catheter based phase stabilized swept source optical coherence tomography (OCT) system was studied for characterization of the strain inside different human ovarian tissue groups. The ovarian tissue samples were periodically compressed with 500 Hz square wave signal along the axial direction between the surface of an unfocused transducer and a glass cover slide. The displacement and corresponding strain were calculated during loading from different locations for each tissue sample. A total of 27 ex vivo ovaries from 16 patients were investigated. Statistically significant difference (p < 0.001) was observed between the average ...
We have estimated the micro-mechanical properties of ovarian tissue using phase-sensitive swept source optical coherence tomography. Ovary samples were mechanically excited by periodical vibration of an ultrasound transducer. The displacement and strain of the tissues were calculated during loading. Significant difference in strain was observed between the normal and malignant ovary groups, which indicates much softer and heterogeneous tissue structure for malignant ovaries. The initial results show that the phase sensitive swept source optical coherence elastography (OCE) can be an effective tool for characterization of stiffness and other micro-mechanical properties of normal and malignant ovarian tissue.
Optical scattering coefficient from ex-vivo unfixed normal and malignant ovarian tissue was quantitatively extracted by fitting optical coherence tomography (OCT) A-line signals to a single scattering model. 1097 average A-line measurements at a wavelength of 1310nm were performed at 108 sites obtained from 18 ovaries. The average scattering coefficient obtained from normal group consisted of 833 measurements from 88 sites was 2.41 mm-1 (±0.59), while the average coefficient obtained from malignant group consisted of 264 measurements from 20 sites was 1.55 mm-1 (±0.46). Using a threshold of 2 mm-1 for each ovary, a sensitivity of 100 ...
