1. Articles from Seung-Yeol Lee

    1-17 of 17
    1. Biophotonic approach for the characterization of initial bitter-rot progression on apple specimens using optical coherence tomography assessments

      Biophotonic approach for the characterization of initial bitter-rot progression on apple specimens using optical coherence tomography assessments

      The tremendous advances achieved in the biophotonics technologies have intensified the necessity for non-invasive modalities that can characterize diverse biological materials with increased sensitivity and resolution. Optical coherence tomography (OCT) is one of the techniques that has been applied for biological applications in medicine and agriculture to identify structural properties. Herein, we report the successful incorporation of OCT for the identification of morphological changes that occur as a result of the bitter rot disease, through continuous detection of structural changes. Detailed inner morphological structural changes occurring in fruit specimens were precisely analyzed as a function of the disease incubation period ...

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    2. Non-Destructive Classification of Diversely Stained Capsicum annuum Seed Specimens of Different Cultivars Using Near-Infrared Imaging Based Optical Intensity Detection

      Non-Destructive Classification of Diversely Stained Capsicum annuum Seed Specimens of Different Cultivars Using Near-Infrared Imaging Based Optical Intensity Detection

      The non-destructive classification of plant materials using optical inspection techniques has been gaining much recent attention in the field of agriculture research. Among them, a near-infrared (NIR) imaging method called optical coherence tomography (OCT) has become a well-known agricultural inspection tool since the last decade. Here we investigated the non-destructive identification capability of OCT to classify diversely stained (with various staining agents) Capsicum annuum seed specimens of different cultivars. A swept source (SS-OCT) system with a spectral band of 1310 nm was used to image unstained control C. annuum seeds along with diversely stained Capsicum seeds, belonging to different cultivar ...

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    3. In Vivo Non-Destructive Monitoring of Capsicum Annuum Seed Growth with Diverse NaCl Concentrations Using Optical Detection Technique

      In Vivo Non-Destructive Monitoring of Capsicum Annuum Seed Growth with Diverse NaCl Concentrations Using Optical Detection Technique

      We demonstrate that optical coherence tomography (OCT) is a plausible optical tool for in vivo detection of plant seeds and its morphological changes during growth. To investigate the direct impact of salt stress on seed germination, the experiment was conducted using Capsicum annuum seeds that were treated with different molar concentrations of NaCl. To determine the optimal concentration for the seed growth, the seeds were monitored for nine consecutive days. In vivo two-dimensional OCT images of the treated seeds were obtained and compared with the images of seeds that were grown using sterile distilled water. The obtained results confirm the ...

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    4. The Validity of the Diagnosis of Plant Leaf Infections Using Non-Destructive Optical Inspection Technique and Depth-Scan Signal Analysis

      The Validity of the Diagnosis of Plant Leaf Infections Using Non-Destructive Optical Inspection Technique and Depth-Scan Signal Analysis

      The applicability of non-destructive optical imaging technique for the diagnosis of infected leaf materials was investigated along with a depth-scan (amplitude depth profile) signal analysis. The experimented plant leaf specimens were collected from persimmon plantations and the specimens were categorized into three groups: healthy leaf specimens, infected leaf specimens, and apparently healthy leaf specimens collected from infected trees. The non-destructive diagnosis was performed using 840 nm spectral domain optical coherence tomography (SD-OCT) system. The obtained OCT results confirmed that the proposed method is capable of identifying morphological differences between healthy specimens from infected specimens and partially infected specimens. Therefore, this ...

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    5. Feature Of The Week 09/17/2017: Non-Destructive Analysis of the Internal Anatomical Structures of Mosquito Specimens Using Optical Coherence Tomography

      Feature Of The Week 09/17/2017: Non-Destructive Analysis of the Internal Anatomical Structures of Mosquito Specimens Using Optical Coherence Tomography

      The study of mosquitoes and analysis of their behavior are of crucial importance in the on-going efforts to control the alarming increase in mosquito-borne diseases. Furthermore, a non-destructive and real-time imaging technique to study the anatomical features of mosquito specimens can greatly aid the study of mosquitoes. In this study, we demonstrate the three-dimensional imaging capabilities of optical coherence tomography (OCT) for structural analysis of Anopheles sinensis mosquitoes. The anatomical features of An. sinensis head, thorax, and abdominal regions, along with the morphology of internal structures, such as foregut, midgut, and hindgut, were studied using OCT imaging. Two-dimensional and three-dimensional ...

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    6. Non-Destructive Analysis of the Internal Anatomical Structures of Mosquito Specimens Using Optical Coherence Tomography

      Non-Destructive Analysis of the Internal Anatomical Structures of Mosquito Specimens Using Optical Coherence Tomography

      The study of mosquitoes and analysis of their behavior are of crucial importance in the on-going efforts to control the alarming increase in mosquito-borne diseases. Furthermore, a non-destructive and real-time imaging technique to study the anatomical features of mosquito specimens can greatly aid the study of mosquitoes. In this study, we demonstrate the three-dimensional imaging capabilities of optical coherence tomography (OCT) for structural analysis of Anopheles sinensis mosquitoes. The anatomical features of An. sinensis head, thorax, and abdominal regions, along with the morphology of internal structures, such as foregut, midgut, and hindgut, were studied using OCT imaging. Two-dimensional and three-dimensional ...

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    7. Application of wearable optical coherence tomography (OCT) and Loop-mediated isothermal amplification (LAMP) techniques for In situ real-time field inspection of apple Marssonina blotch disease

      Application of wearable optical coherence tomography (OCT) and Loop-mediated isothermal amplification (LAMP) techniques for In situ real-time field inspection of apple Marssonina blotch disease

      Here we describe the possible application of optical coherence tomography (OCT) to inspect Marssonina coronaria infected apple blotch disease of in situ apple leaves. To fulfill the in situ field inspection requirement, we developed a compact wearable OCT system. For the confirmation of OCT results, simultaneous experiment was performed in realtime using loop-mediated isothermal amplification (LAMP), which is frequently used in agriculture. LAMP method was developed as an alternative approach for the inspection of disease. We performed field inspection for 30 consecutive days, and all the acquired results from both OCT and lamp were compared to confirm the correlation. A ...

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    8. In vivo observation of metamorphosis of Plodia interpunctella Hübner using three-dimensional optical coherence tomography

      In vivo observation of metamorphosis of Plodia interpunctella Hübner using three-dimensional optical coherence tomography

      Morphological assessment and three-dimensional reconstructions of internal structures of Plodia interpunctella Hübner during metamorphosis stages were experimentally demonstrated using optical coherence tomography (OCT) for the first time. The conventional, complex sectioning methods were significantly simplified owing to the non-invasive three-dimensional imaging capability of OCT. Further, this study demonstrates the use of OCT as a non-invasive detection tool for in vivo morphological observation of metamorphosis stages to gain a better understanding about the growth of internal organs, which can be considered a useful discovery in the field of entomology. Thus, the metamorphosis stages starting from the larva, three pupa stages ...

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    9. Optical coherence tomography-integrated, wearable (backpack-type), compact diagnostic imaging modality for in situ leaf quality assessment

      Optical coherence tomography-integrated, wearable (backpack-type), compact diagnostic imaging modality for in situ leaf quality assessment

      We developed a compact, wearable diagnostic imaging modality employing optical coherence tomography for in situ plant leaf quality assessments. This system is capable of diagnosing infected leaves at the initial disease stages. Our system is a versatile backpack-type imaging modality with a compact spectrometer, miniature computer, rechargeable power source, and handheld inspection probe. This method enhances real-time in situ specimen inspection through direct implementation of the imaging modality in a plantation. To evaluate the initial performance, field experiments were conducted in apple, pear, and persimmon plantations. Based on the obtained results, we can conclude that the developed imaging modality can ...

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    10. Optical sensing method to analyze germination rate of Capsicum annum seeds treated with growth-promoting chemical compounds using optical coherence tomography

      Optical sensing method to analyze germination rate of Capsicum annum seeds treated with growth-promoting chemical compounds using optical coherence tomography

      Seed germination rate differs based on chemical treatments, and nondestructive measurements of germination rate have become an essential requirement in the field of agriculture. Seed scientists and other biologists are interested in optical sensing technologies-based biological discoveries due to nondestructive detection capability. Optical coherence tomography (OCT) has recently emerged as a powerful method for biological and plant material discoveries. We report an extended application of OCT by monitoring the germination rate acceleration of chemically primed seeds. To validate the versatility of the method, Capsicum annum seeds were primed using three chemical compounds: sterile distilled water (SDW), butandiol, and 1-hexadecene. Monitoring ...

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    11. Non-invasive method and apparatus for screening high-quality seeds

      Non-invasive method and apparatus for screening high-quality seeds

      Disclosed is a method and apparatus for the non-invasive selection of high-quality seeds, based on optical coherence tomography, by which pathogen-infected and pathogen-free seeds can be discriminated in a non-invasive manner. The apparatus is operated by the processes of scanning a seed of diagnostic interest in a non-invasive manner using an optical coherence tomographic unit; processing interference signals of the scanned tomographic images to produce tomographic image data of the seed of diagnostic interest; analyzing the tomographic image data; comparing the analyzed tomographic image data with preset reference tomographic image data; diagnosing the seed of diagnostic interest as a pathogen-infected ...

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    12. Method and device for early non-invasive diagnosis of fruit tree disease

      Method and device for early non-invasive diagnosis of fruit tree disease

      The present invention relates to a non-invasive early diagnostic method and device that allows an early diagnosis of Marssonina blotch disease, which infects apple tree leaves, before the occurrence of lesions to enable early prevention of apple Marssonina blotch disease. The method and device of the present invention comprise the following steps: irradiating a broadband light source on an apple tree leaf undergoing diagnosis by means of optical coherence tomography; detecting interference signals from light reflected from the apple tree leaf undergoing diagnosis and light reflected from a reference arm; signal processing the interference signals, which have been detected through ...

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    13. Non-Invasive Method and Apparatus for Screening High-Quality Seeds

      Non-Invasive Method and Apparatus for Screening High-Quality Seeds

      Disclosed is a method and apparatus for the non-invasive selection of high-quality seeds, based on optical coherence tomography, by which pathogen-infected and pathogen-free seeds can be discriminated in a non-invasive manner. The apparatus is operated by the processes of scanning a seed of diagnostic interest in a non-invasive manner using an optical coherence tomographic unit; processing interference signals of the scanned tomographic images to produce tomographic image data of the seed of diagnostic interest; analyzing the tomographic image data; comparing the analyzed tomographic image data with preset reference tomographic image data; diagnosing the seed of diagnostic interest as a pathogen-infected ...

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    14. Method And Device For Early Non-Invasive Diagnose Of Fruit Tree Disease

      Method And Device For Early Non-Invasive Diagnose Of Fruit Tree Disease

      The present invention relates to a non-invasive early diagnostic method and device that allows an early diagnosis of Marssonina blotch disease, which infects apple tree leaves, before the occurrence of lesions to enable early prevention of apple Marssonina blotch disease. The method and device of the present invention comprise the following steps: irradiating a broadband light source on an apple tree leaf undergoing diagnosis by means of optical coherence tomography; detecting interference signals from light reflected from the apple tree leaf undergoing diagnosis and light reflected from a reference arm; signal processing the interference signals, which have been detected through ...

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    15. Application of optical coherence tomography to detect Cucumber green mottle mosaic virus (CGMMV) infected cucumber seed

      Application of optical coherence tomography to detect Cucumber green mottle mosaic virus (CGMMV) infected cucumber seed

      Abstract  In this study, we distinguished Cucumber green mottle mosaic virus (CGMMV) infected seeds from healthy seeds using optical coherence tomography (OCT). Two dimensional OCT images and stereo micrographs revealed that the infected seeds had narrow gap between the seed coat and endosperm that were not present in the healthy seeds. Three dimensional OCT images confirmed that the narrow gaps were present in the inner structure of overall the infected seeds. A-scan analysis was also performed to calculate the distance from the seed coat to the endosperm. The results revealed a difference in the width of the gap of about ...

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    16. The Application of Optical Coherence Tomography in the Diagnosis of Marssonina Blotch in Apple Leaves

      The Application of Optical Coherence Tomography in the Diagnosis of Marssonina Blotch in Apple Leaves

      In this study we investigate the use of 2D and 3D scanning optical coherence tomography (OCT) technology for use in apple blotch diagnosis. In order to test the possible application of OCT as a detection tool for apple trees affected by Marssonina coronaria, we conducted several experiments and compared the results from both healthy and infected leaves. Using OCT, we found several distinctive features in the subsurface boundary regions of both the diseased and healthy leaves. Our results indicate that leaves from diseased trees, while still appearing healthy, can be affected by M. coronaria. The A-scan analysis method confirmed that ...

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    17. Optical Sensing Method for Screening Disease in Melon Seeds by Using Optical Coherence Tomography

      Optical Sensing Method for Screening Disease in Melon Seeds by Using Optical Coherence Tomography

      We report a noble optical sensing method to diagnose seed abnormalities using optical coherence tomography (OCT). Melon seeds infected with Cucumber green mottle mosaic virus (CGMMV) were scanned by OCT. The cross-sectional sensed area of the abnormal seeds showed an additional subsurface layer under the surface which is not found in normal seeds. The presence of CGMMV in the sample was examined by a blind test (n = 140) and compared by the reverse transcription-polymerase chain reaction. The abnormal layers (n = 40) were quantitatively investigated using A-scan sensing analysis and statistical method. By utilizing 3D OCT image reconstruction, we confirmed the ...

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    1-17 of 17
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    1. (16 articles) Jeehyun Kim
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    5. (7 articles) Changho Lee
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    Optical Sensing Method for Screening Disease in Melon Seeds by Using Optical Coherence Tomography The Application of Optical Coherence Tomography in the Diagnosis of Marssonina Blotch in Apple Leaves Application of optical coherence tomography to detect Cucumber green mottle mosaic virus (CGMMV) infected cucumber seed Non-Invasive Method and Apparatus for Screening High-Quality Seeds Method And Device For Early Non-Invasive Diagnose Of Fruit Tree Disease Optical sensing method to analyze germination rate of Capsicum annum seeds treated with growth-promoting chemical compounds using optical coherence tomography Optical coherence tomography-integrated, wearable (backpack-type), compact diagnostic imaging modality for in situ leaf quality assessment Application of wearable optical coherence tomography (OCT) and Loop-mediated isothermal amplification (LAMP) techniques for In situ real-time field inspection of apple Marssonina blotch disease Feature Of The Week 09/17/2017: Non-Destructive Analysis of the Internal Anatomical Structures of Mosquito Specimens Using Optical Coherence Tomography The Validity of the Diagnosis of Plant Leaf Infections Using Non-Destructive Optical Inspection Technique and Depth-Scan Signal Analysis Optical coherence tomography angiography of foveal neovascularisation in proliferative diabetic retinopathy Treating port wine stain birthmarks using dynamic optical coherence tomography-guided setting