1. Articles from Kuang-Yu Hsu

    1-11 of 11
    1. Rapid pseudo-H&E imaging using a fluorescence-inbuilt optical coherence microscopic imaging system

      Rapid pseudo-H&E imaging using a fluorescence-inbuilt optical coherence microscopic imaging system

      A technique using Linnik-based optical coherence microscopy (OCM), with built-in fluorescence microscopy (FM), is demonstrated here to describe cellular-level morphology for fresh porcine and biobank tissue specimens. The proposed method utilizes color-coding to generate digital pseudo-H&E (p-H&E) images. Using the same camera, colocalized FM images are merged with corresponding morphological OCM images using a 24-bit RGB composition process to generate position-matched p-H&E images. From receipt of dissected fresh tissue piece to generation of stitched images, the total processing time is <15 min for a 1-cm 2 specimen, which is on average two times faster than frozen-section H ...

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    2. Feature Of The Week 9/28/14: National Taiwan University Reports on Full-depth epidermis tomography using a Mirau-based full-field OCT

      Feature Of The Week 9/28/14: National Taiwan University Reports on Full-depth epidermis tomography using a Mirau-based full-field OCT

      All living things are made of cells. Most living organisms are single cells; others, such as human bodies, are vast multicellular cities with an aggregation of more than 10 billion cells. Non-invasive and high speed imaging of cells and tissues with sub-micron resolution could help unveil functions of living organisms, and facilitate disease/cancer diagnosis, new drug development, etc. Such an imaging modality is one of the key technologies to detect disease and cancer in the early stage so that the ever-escalating medical cost could be controlled. At the same time, biomedical discovery often originated from technology advancement. Optical coherence ...

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    3. Full-depth epidermis tomography using a Mirau-based full-field optical coherence tomography

      Full-depth epidermis tomography using a Mirau-based full-field optical coherence tomography

      With a Gaussian-like broadband light source from high brightness Ce 3+ :YAG single-clad crystal fiber, a full-field optical coherence tomography using a home-designed Mirau objective realized high quality images of in vivo and excised skin tissues. With a 40 × silicone-oil-immersion Mirau objective, the achieved spatial resolutions in axial and lateral directions were 0.9 and 0.51 μm, respectively. Such a high spatial resolution enables the separation of lamellar structure of the full epidermis in both the cross-sectional and en face planes. The number of layers of stratum corneum and its thickness were quantitatively measured. This label free and non-invasive ...

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    4. Spectroscopic measurement of absorptive thin films by Spectral-Domain Optical Coherence Tomography

      Spectroscopic measurement of absorptive thin films by Spectral-Domain Optical Coherence Tomography

      A non-invasive method for measuring the refractive index, extinction coefficient and film thickness of absorptive thin films using spectral-domain optical coherent tomography is proposed, analyzed and experimentally demonstrated. Such an optical system employing a normal-incident beam of light exhibits a high spatial resolution. There are no mechanical moving parts involved for the measurement except the transversal scanning module for the measurement at various transversal locations. The method was experimentally demonstrated on two absorptive thin-film samples coated on transparent glass substrates. The refractive index and extinction coefficient spectra from 510 to 580 nm wavelength range and film thickness were simultaneously measured ...

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    5. Three-dimensional optical coherence tomography confocal imaging apparatus

      Three-dimensional optical coherence tomography confocal imaging apparatus

      A 3D OCT confocal imaging apparatus includes a light source module for providing an illumination beam with wider bandwidth from a crystal fiber; a reference source module; a pickup module; a beam splitter; an optical filter; and a sensor module. When the illumination beam illuminates a sample, a pickup objective lens and a piezoelectric actuator of the pickup module together provide an image beam scanning the sample in depth direction. The image beam and a reference beam from the reference source module together form an interference image beam, which is converted by a photosensor into a coherence image electric signal ...

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    6. Optical imaging apparatus and method

      Optical imaging apparatus and method

      The present invention relates to an optical imaging apparatus and a method, and more particularly to an optical imaging apparatus and a method with short coherence length optical source. The apparatus comprises an optical source with a plurality of outputs for providing a reference light and a sample light; a sample probe module for leading the sample light to a sample, and leading an information light out; an interference module for leading the reference light to a photo detector, and leading the information light to the photo detector; and a signal processing unit electrically coupled to the photo detector; wherein ...

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    7. Mirau-based full-field time-domain optical coherence tomography using Ce3+ :YAG crystal fiber light source

      Mirau-based full-field time-domain optical coherence tomography using Ce3+ :YAG crystal fiber light source

      Based on single-objective construction utilizing high brightness Ce 3+ :YAG single-clad crystal fiber light source, this Mirau-based full-field time-domain optical coherence tomography with circular polarization incident light represents deeper penetration in scattering medium. Using objective-changeable ability of home-designed Mirau objective, this system provides different applications, like biological tissue and single cells, by different spatial resolution with corresponding dynamics. High quality image relying on less ghost image and near common-path interference was demonstrated under this compact and power-stable system.

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    8. Diode-Laser-Pumped Glass-Clad Ti:Sapphire Crystal-Fiber-Based Broadband Light Source

      Diode-Laser-Pumped Glass-Clad Ti:Sapphire Crystal-Fiber-Based Broadband Light Source

      Glass-clad TiAlO3 crystal fibers were grown using the codrawing laser-heated pedestal growth method. Both single-clad and double-clad structures were attempted. Using a 446-nm laser diode as the pump, 2.45 mW of collimated broadband emission was generated with a 3-dB bandwidth of 180 nm. As much as 7.7 mW of output power with a 3-dB bandwidth of 182 nm was generated using a 532-nm solid-state pump laser. The broadband light source is advantageous to use in optical coherence tomography with an axial resolution of 1.5 um in air and a low image pixel crosstalk.

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    9. Bidirectionally pumped Cr^4+:YAG crystal fiber light source for optical coherence tomography

      Bidirectionally pumped Cr^4+:YAG crystal fiber light source for optical coherence tomography
      A broadband Cr4+:YAG double-clad crystal fiber light source with a collimated output power of 3.15mW was demonstrated by using a bidirectional pump scheme. The bidirectional pump scheme increased the pumping efficiency and reduced the thermal problem along the fiber. Good agreement on the output power of the broadband fluorescence was achieved between experiment and theory, which showed a peak net gain coefficient of 0.03cm−1 at the center wavelength near 1380nm. The 3dB bandwidth of 222nm with a Gaussian-like spectrum makes it eminently suitable for broadband interferometric technique to have low image cross talk. The calculated interference ...
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    10. High-Luminance White-Light Point Source Using Ce,Sm : YAG Double-Clad Crystal Fiber

      High-Luminance White-Light Point Source Using Ce,Sm : YAG Double-Clad Crystal Fiber
      Ce and Sm ions codoped yttrium aluminum garnet (Ce,Sm : YAG) double-clad crystal fibers (DCFs) were successfully fabricated using the codrawing laser-heated pedestal growth method. At a pump wavelength of 446 nm, white-light point source was produced from a 10-$mu{hbox {m}}$-core DCF. The amplified spontaneous emissions and the residual pump resulted in a directional high brightness point source. A luminance of $4.5times 10 ^{9}~hbox{cd}/hbox{m} ^{2}$ was generated. The luminous flux of the point white light was 6.2 lm. When coupled to a 200-$mu{hbox {m}}$-core multimode fiber, the output ...
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    11. Ce^3+:YAG double-clad crystal-fiber-based optical coherence tomography on fish cornea

      Ce^3+:YAG double-clad crystal-fiber-based optical coherence tomography on fish cornea
      A Ce3+:YAG double-clad crystal fiber (DCF) visible emission was used as the light source for optical coherence tomography (OCT). The visible emission was produced from a 10 μm core DCF pumped by a diode laser. The broadband emission and short central wavelength of this light source enabled the realization of 1.5 μm axial resolution in air. The relatively clean spectrum reduced the side lobe of its point-spread function, and therefore facilitated the generation of a high-quality image with less crosstalk between adjacent image pixels. As a demonstration, an Aplocheilus lineatus goldfish was experimented on to map out the ...
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    1-11 of 11
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    1. (11 articles) National Taiwan University
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    Ce^3+:YAG double-clad crystal-fiber-based optical coherence tomography on fish cornea High-Luminance White-Light Point Source Using Ce,Sm : YAG Double-Clad Crystal Fiber Bidirectionally pumped Cr^4+:YAG crystal fiber light source for optical coherence tomography Diode-Laser-Pumped Glass-Clad Ti:Sapphire Crystal-Fiber-Based Broadband Light Source Mirau-based full-field time-domain optical coherence tomography using Ce3+ :YAG crystal fiber light source Optical imaging apparatus and method Three-dimensional optical coherence tomography confocal imaging apparatus Spectroscopic measurement of absorptive thin films by Spectral-Domain Optical Coherence Tomography Full-depth epidermis tomography using a Mirau-based full-field optical coherence tomography Feature Of The Week 9/28/14: National Taiwan University Reports on Full-depth epidermis tomography using a Mirau-based full-field OCT Normative data and associations of Optical Coherence Tomography Angiography measurements of the macula: The Singapore Malay Eye Study Automatic Segmentation and Measurement of Choroid Layer in High Myopia for OCT Imaging Using Deep Learning