1. Articles from Lilla Knels

    1-6 of 6
    1. Investigation of alveolar tissue deformations using OCT combined with fluorescence microscopy

      Investigation of alveolar tissue deformations using OCT combined with fluorescence microscopy

      In critical care medicine, artificial ventilation is a life saving tool providing sufficient blood oxygenation to patients suffering from respiratory failure. Essential for their survival is the use of protective ventilation strategies to prevent further lung damage due to ventilator induced lung injury (VILI). Since there is only little known about implications of lung tissue overdistension on the alveolar level, especially in the case of diseased lungs, this research deals with the investigation of lung tissue deformation on a microscale. A combined setup utilizing optical coherence tomography (OCT) and confocal fluorescence microscopy, is used to study the elastic behavior of ...

      Read Full Article
    2. Three-dimensional functional imaging of lung parenchyma using optical coherence tomography combined with confocal fluorescence microscopy

      Three-dimensional functional imaging of lung parenchyma using optical coherence tomography combined with confocal fluorescence microscopy

      Optical coherence tomography (OCT), as a non-invasive technique for studying tissue morphology, is widely used in in vivo studies, requiring high resolution and fast three-dimensional imaging. Based on light scattering it reveals micrometer sized substructures of the samples due to changes in their optical properties and therefore allows quantification of the specimen's geometry. Utilizing fluorescence microscopy further information can be obtained from molecular compositions embedded in the investigated object. Fluorescent markers, specifically binding to the substance of interest, reveal the sample's chemical structure and give rise to functional studies. This research presents the application of a combined OCT ...

      Read Full Article
    3. Combining optical coherence tomography with fluorescence microscopy: a closer look into tissue

      Combining optical coherence tomography with fluorescence microscopy: a closer look into tissue

      Optical coherence tomography (OCT) is a technique, capable of high resolution and non-invasive 3D imaging in vivo by detection of backscattered light from cellular and sub cellular structures. Due to visualization of micrometer sized tissue constituents and high penetration depths of up to 2 mm, it is already well established in medical fields like ophthalmology and dermatology. Laser scanning confocal microscopy (LSCM), on the contrary, gives further information on structural tissue components stained with suitable dyes. In combination, these two methods yield three dimensional and high resolution data providing geometrical and structural details of tissue. In this study, we present ...

      Read Full Article
    4. Three-dimensional Fourier domain optical coherence tomography in vivo imaging of alveolar tissue in the intact thorax using the parietal pleura as a window

      Three-dimensional Fourier domain optical coherence tomography in vivo imaging of alveolar tissue in the intact thorax using the parietal pleura as a window
      In vivo determination of 3-D and dynamic geometries of alveolar structures with adequate resolution is essential for developing numerical models of the lung. A thorax window is prepared in anesthetized rabbits by removal of muscle tissue between the third and fourth rib without harming the parietal pleura. The transparent parietal pleura allows contact-free imaging by intravital microscopy IVM and 3-D optical coherence tomography 3-D OCT. We demonstrate that dislocation of the lung surface is small enough to observe identical regions in the expiratory and inspiratory plateau phase, and that OCT in this animal model is suitable for generating 3-D geometry ...
      Read Full Article
    5. Improved three-dimensional Fourier domain optical coherence tomography by index matching in alveolar structures

      Three-dimensional Fourier domain optical coherence tomography (3-D FDOCT) is used to demonstrate that perfusion fixation with a mixture of glutaraldehyde and paraformaldehyde does not alter the geometry of subpleural lung parenchyma in isolated and perfused rabbit lungs. This is confirmed by simultaneous imaging of lung parenchyma with intravital microscopy. To eliminate the diffraction index interfaces between alveolar pockets and walls, we fill the fixed lungs with ethanol by perfusing with gradually increasing concentrations. This bottom-up filling process leaves no remaining air bubbles in the alveolar structures, thus drastically improving the resolution and penetration depth of 3-D FDOCT imaging. We observe ...
      Read Full Article
    6. Simultaneous three-dimensional optical coherence tomography and intravital microscopy for imaging subpleural pulmonary alveoli in isolated rabbit lungs

      There is a growing interest in analyzing lung mechanics at the level of the alveoli in order to understand stress-related pathogenesis and possibly avoid ventilator associated lung injury. Emerging quantitative models to simulate fluid mechanics and the associated stresses and strains on delicate alveolar walls require realistic quantitative input on alveolar geometry and its dynamics during ventilation. Here, three-dimensional optical coherence tomography (OCT) and conventional intravital microscopy are joined in one setup to investigate the geometric changes of subpleural alveoli during stepwise pressure increase and release in an isolated and perfused rabbit lung model. We describe good qualitative agreement and ...
      Read Full Article
    1-6 of 6
  1. Categories

    1. Applications:

      Art, Cardiology, Dentistry, Dermatology, Developmental Biology, Gastroenterology, Gynecology, Microscopy, NDE/NDT, Neurology, Oncology, Ophthalmology, Other Non-Medical, Otolaryngology, Pulmonology, Urology
    2. Business News:

      Acquisition, Clinical Trials, Funding, Other Business News, Partnership, Patents
    3. Technology:

      Broadband Sources, Probes, Tunable Sources
    4. Miscellaneous:

      Jobs & Studentships, Student Theses, Textbooks
  2. Topics in the News

    1. (6 articles) Dresden University of Technology
    2. (6 articles) Edmund Koch
    3. (6 articles) Sven Meissner
    4. (3 articles) Maria Gaertner
    5. (3 articles) Peter Cimalla
    6. (1 articles) University of Toronto
    7. (1 articles) Alexander Krüger
    8. (1 articles) Academic Medical Center at the University of Amsterdam
    9. (1 articles) Jichi Medical University
    10. (1 articles) University of Milan
    11. (1 articles) Bern University Hospital
    12. (1 articles) Capital Medical University
    13. (1 articles) Nanjing University of Science and Technology
    14. (1 articles) Martin S. Zinkernagel
    15. (1 articles) Junya Ako
    16. (1 articles) Frank D. Verbraak
    17. (1 articles) Giovanni Staurenghi
  3. Popular Articles

  4. Picture Gallery

    Three-dimensional Fourier domain optical coherence tomography in vivo imaging of alveolar tissue in the intact thorax using the parietal pleura as a window Combining optical coherence tomography with fluorescence microscopy: a closer look into tissue Three-dimensional functional imaging of lung parenchyma using optical coherence tomography combined with confocal fluorescence microscopy Investigation of alveolar tissue deformations using OCT combined with fluorescence microscopy Correlation between optical coherence tomography, multifocal electroretinogram findings and visual acuity in diabetic macular edema Ciliary body length revisited by anterior segment optical coherence tomography: implications for safe access to the pars plana for intravitreal injections Evaluation of retinal vascular structure after epiretinal membrane surgery by optical coherence tomography angiography Bivalirudin vs. Heparin on Radial Artery Thrombosis during Transradial Coronary Intervention: An Optical Coherence Tomography Study Superficial Calcification With Rotund Shape Is Associated With Carotid Plaque Rupture: An Optical Coherence Tomography Study Longitudinal retinal layer changes in preclinical Alzheimer’s disease Clinical expert consensus document on rotational atherectomy from the Japanese association of cardiovascular intervention and therapeutics Optical coherence tomography features of choroidal neovascularization and their correlation with age, gender and underlying disease