1. Articles from Chao-Wei Chen

    1-14 of 14
    1. Depth-resolved imaging of colon tumor using optical coherence tomography and fluorescence laminar optical tomography

      Depth-resolved imaging of colon tumor using optical coherence tomography and fluorescence laminar optical tomography

      Early detection of neoplastic changes remains a critical challenge in clinical cancer diagnosis and treatment. Many cancers arise from epithelial layers such as those of the gastrointestinal (GI) tract. Current standard endoscopic technology is difficult to detect the subsurface lesions. In this research, we investigated the feasibility of a novel multi-modal optical imaging approach including high-resolution optical coherence tomography (OCT) and high-sensitivity fluorescence laminar optical tomography (FLOT) for structural and molecular imaging. The C57BL/6J-Apc Min /J mice were imaged using OCT and FLOT, and the correlated histopathological diagnosis was obtained. Quantitative structural (scattering coefficient) and molecular (relative enzyme activity ...

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    2. Three dimensional mesoscale analysis of translamellar cross-bridge morphologies in the annulus fibrosus using optical coherence tomography

      Three dimensional mesoscale analysis of translamellar cross-bridge morphologies in the annulus fibrosus using optical coherence tomography

      The defining characteristic of the annulus fibrosus (AF) of the intervertebral disc (IVD) has long been the lamellar structures that consist of highly ordered collagen fibers arranged in alternating oblique angles from one layer to the next. However, a series of recent histologic studies have demonstrated that AF lamellae contain elastin- and type VI collagen-rich secondary “cross-bridge” structures across lamellae. In this study, we use optical coherence tomography (OCT) to elucidate the three-dimensional (3-D) morphologies of these translamellar cross-bridges in AF tissues. Mesoscale volumetric images by OCT revealed a 3-D network of heterogeneously distributed cross-bridges. The results of this study ...

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    3. Variations in optical coherence tomography resolution and uniformity: a multi-system performance comparison

      Variations in optical coherence tomography resolution and uniformity: a multi-system performance comparison

      Point spread function (PSF) phantoms based on unstructured distributions of sub-resolution particles in a transparent matrix have been demonstrated as a useful tool for evaluating resolution and its spatial variation across image volumes in optical coherence tomography (OCT) systems. Measurements based on PSF phantoms have the potential to become a standard test method for consistent, objective and quantitative inter-comparison of OCT system performance. Towards this end, we have evaluated three PSF phantoms and investigated their ability to compare the performance of four OCT systems. The phantoms are based on 260-nm-diameter gold nanoshells, 400-nm-diameter iron oxide particles and 1.5-micron-diameter silica ...

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    4. Multilayer thin-film phantoms for axial contrast transfer function measurement in optical coherence tomography

      Multilayer thin-film phantoms for axial contrast transfer function measurement in optical coherence tomography

      In optical coherence tomography (OCT), axial resolution is one of the most critical parameters impacting image quality. It is commonly measured by determining the point spread function (PSF) based on a specular surface reflection. The contrast transfer function (CTF) provides more insights into an imaging system’s resolving characteristics and can be readily generated in a system-independent manner, without consideration for image pixel size. In this study, we developed a test method for determination of CTF based on multi-layer, thin-film phantoms, evaluated using spectral- and time-domain OCT platforms with different axial resolution values. Phantoms representing six spatial frequencies were fabricated ...

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    5. Multi-system comparison of optical coherence tomography performance with point spread function phantoms

      Multi-system comparison of optical coherence tomography performance with point spread function phantoms

      Point spread function (PSF) phantoms based on unstructured distributions of sub-resolution particles in a transparent matrix have proven effective for evaluating resolution and its spatial variation in optical coherence tomography (OCT) systems. Measurements based on PSF phantoms have the potential to become a standard test method for consistent, objective and quantitative inter-comparison of OCT system performance. Towards this end, we have evaluated three PSF phantoms and investigated their ability to compare the performance of four OCT systems. The phantoms are based on 260-nm-diameter gold nanoshells, submicron-diameter iron oxide particles and 1.5-micron-diameter silica particles. The OCT systems included spectral-domain and ...

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    6. Feature Of The Week 9/25/11: University of Maryland and NIH Researchers Investigate Combined OCT and Fluorescence Imaging for Improved Colon Cancer Detection

      Feature Of The Week 9/25/11: University of Maryland and NIH Researchers Investigate Combined OCT and Fluorescence Imaging for Improved Colon Cancer Detection
      Optical Coherence Tomography (OCT) offers high-resolution imaging of the tissue microstructure. But there are numerous limitations to OCT including providing direct molecular imaging capability. A popular research thrust has recently been to combine OCT with other imaging technology such as Raman, Fluorescence, and other molecular imaging techniques to achieve a multi-modality imaging capability. This multi-modality imaging offers improved capabilities including all the normal OCT benefits as well as allowing the molecular imaging signals to be spatial registered within the tissue microstructure. This can lead to significant improvements in clinical capability including cancer detection. Researchers at the University of Maryland and ...
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    7. Rh-I-UEA-1 Polymerized Liposomes Target and Image Adenomatous Polyps in the APCMin/+ Mouse Using Optical Colonography

      Rh-I-UEA-1 Polymerized Liposomes Target and Image Adenomatous Polyps in the APCMin/+ Mouse Using Optical Colonography
      Mutated adenomatous polyposis coli (APC) genes predispose transformations to neoplasia, progressing to colorectal carcinoma. Early detection facilitates clinical management and therapy. Novel lectin-mediated polymerized targeted liposomes (Rh-I-UEA-1), with polyp specificity and incorporated imaging agents were fabricated to locate and image adenomatous polyps in APCMin/+ mice. The biomarker α-l-fucose covalently joins the liposomal conjugated lectin Ulexeuropaeus agglutinin (UEA-1), via glycosidic linkage to the polyp mucin layer. Multispectral optical imaging (MSI) corroborated a global perspective of specific binding (rhodamine B 532 nm emission, 590–620 nm excitation) of targeted Rh-I-UEA-1 polymerized liposomes to polyps with 1.4-fold labeling efficiency. High-resolution coregistered optical ...
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    8. Feature Of The Week 9/26/10: Researchers from University of Maryland Demonstrated Multimodality Optical Coherence Tomography and Fluorescence Laminar Optical Tomography Imaging

      Feature Of The Week 9/26/10: Researchers from University of Maryland Demonstrated Multimodality Optical Coherence Tomography and Fluorescence Laminar Optical Tomography Imaging
      Feature Of The Week 9/26/10: Multimodal imaging represents one of the current trends in the development of biophotonics imaging technologies for the integration of molecular and physiological information with structural information, enabling in vivo visualization and quantification of disease biomarkers and their progression over time. Multimodal imaging therefore improves the understanding of disease development, enables early diagnosis, and ultimately enhances treatment efficacy. This work presents an integration of optical coherence tomography (OCT) and fluorescence laminar optical tomography (FLOT), which combines the advantages of high-resolution structural imaging from OCT with high-sensitivity functional imaging from FLOT. This platform provides contextual ...
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    9. Macroporous Hydrogel Scaffolds and Their Characterization By Optical Coherence Tomography

      Macroporous Hydrogel Scaffolds and Their Characterization By Optical Coherence Tomography
      A simple porogen-leaching method to fabricate macroporous cyclic acetal hydrogel cell scaffolds is presented. Optical coherence tomography (OCT) was applied for nondestructive imaging and quantitative characterization of the scaffold structures. High-resolution OCT reveals the microstructures of the engineered tissue scaffolds in three dimensions. It also enables subsequent image processing to investigate quantitatively several key morphological design parameters for macroporous scaffolds, including the volume porosity, pore interconnectivity, and pore size. Two image-processing algorithms were adapted: three-dimensional labeling was applied to assess the interconnectivity, and erosion was applied to assess the pore size. Scaffolds with different design parameters were imaged, characterized, and ...
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    10. Integrated Optical Coherence Tomography (OCT) and Fluorescence Laminar Optical Tomography (FLOT) for Depth-Resolved Subsurface Cancer Imaging

      Integrated Optical Coherence Tomography (OCT) and Fluorescence Laminar Optical Tomography (FLOT) for Depth-Resolved Subsurface Cancer Imaging
      We developed a combined optical coherence tomography (OCT) and fluorescence laminar optical tomography (FLOT) system for co-registered depth-resolved structural and molecular imaging. Experimental results using a mouse model with human breast cancer xenograft are presented.
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    11. Investigation of pore structure and cell distribution in EH-PEG hydrogel scaffold using optical coherence tomography and fluorescence microscopy

      Investigation of pore structure and cell distribution in EH-PEG hydrogel scaffold using optical coherence tomography and fluorescence microscopy
      Macroporous EH-PEG hydrogels fabricated by porogen-leaching method are characterized by optical coherence tomography (OCT). High-resolution OCT visualizes the microstructures of the engineered tissue scaffolds in threedimensions. It also enables subsequent image processing to investigate several key morphological design parameters for macroporous scaffolds. Image processing algorithms are then presented to automatically quantify the pore size, porosity, and pore interconnectivity. The results indicated that those parameters highly depend on the porogen size. Further, fluorescence imaging was conducted to monitor the population of labeled human mesenchymal stem cells (hMSCs) loaded on the surface of the scaffolds. The results revealed the hMSCs' viability as ...
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    12. Co-registered optical coherence tomography and fluorescence molecular imaging for simultaneous morphological and molecular imaging

      Abstract. Optical coherence tomography (OCT) provides high-resolution, cross-sectional imaging of tissue microstructure in situ and in real time, while fluorescence molecular imaging (FMI) enables the visualization of basic molecular processes. There is a great deal of interest in combining these two modalities so that the tissue's structural and molecular information can be obtained simultaneously. This could greatly benefit biomedical applications such as detecting early diseases and monitoring therapeutic interventions. In this research, an optical system that combines OCT and FMI was developed. The system demonstrated that it could co-register en face OCT and FMI images with a 2.4 ...
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    13. Feature Of The Week 9/6/09: Automated Quantification of Microstructural Dimensions of the Human Kidney using Optical Coherence Tomography

      Feature Of The Week 9/6/09: Automated Quantification of Microstructural Dimensions of the Human Kidney using Optical Coherence Tomography
      Feature Of The Week 9/6/09: Optical coherence tomography (OCT) is a rapidly emerging imaging modality that can non-invasively provide cross-sectional, high-resolution images of tissue morphology in situ and in real-time. Recent development in high-speed OCT enables the acquisition of a large amount of three-dimensional image d
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    14. Automated quantification of microstructural dimensions of the human kidney using optical coherence tomography (OCT)

      Automated quantification of microstructural dimensions of the human kidney using optical coherence tomography (OCT)
      Optical coherence tomography (OCT) is a rapidly emerging imaging modality that can non-invasively provide cross-sectional, highresolution images of tissue morphology in situ and in real-time. We previously demonstrated that OCT is capable of visualizing characteristic kidney anatomic structures, including blood vessels, uriniferous tubules,glomeruli, and renal capsules on a Munich–Wistar rat model. Because the viability of a donor kidney is closely correlated with its tubular morphology, and a large amount of image datasets are expected when using OCT to scan the entire kidney to provide a global assessment of its viability, it is necessary to develop automatic image analysis ...
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    1-14 of 14
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  2. Topics in the News

    1. (14 articles) University of Maryland
    2. (14 articles) Chao-Wei Chen
    3. (13 articles) Yu Chen
    4. (7 articles) Shuai Yuan
    5. (5 articles) Jeremiah Wierwille
    6. (4 articles) National Institutes of Health
    7. (4 articles) Alex E. Cable
    8. (4 articles) James Y. Jiang
    9. (4 articles) Thorlabs
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    12. (1 articles) University of Groningen
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    Automated quantification of microstructural dimensions of the human kidney using optical coherence tomography (OCT) Feature Of The Week 9/6/09: Automated Quantification of Microstructural Dimensions of the Human Kidney using Optical Coherence Tomography Investigation of pore structure and cell distribution in EH-PEG hydrogel scaffold using optical coherence tomography and fluorescence microscopy Integrated Optical Coherence Tomography (OCT) and Fluorescence Laminar Optical Tomography (FLOT) for Depth-Resolved Subsurface Cancer Imaging Macroporous Hydrogel Scaffolds and Their Characterization By Optical Coherence Tomography Feature Of The Week 9/26/10: Researchers from University of Maryland Demonstrated Multimodality Optical Coherence Tomography and Fluorescence Laminar Optical Tomography Imaging Rh-I-UEA-1 Polymerized Liposomes Target and Image Adenomatous Polyps in the APCMin/+ Mouse Using Optical Colonography Feature Of The Week 9/25/11: University of Maryland and NIH Researchers Investigate Combined OCT and Fluorescence Imaging for Improved Colon Cancer Detection Multi-system comparison of optical coherence tomography performance with point spread function phantoms Long-Term Arterial Remodeling After Bioresorbable Scaffold Implantation 4-Year Follow-up of Quantitative Coronary Angiography, Histology and Optical Coherence Tomography Visualization of Bacterial Colonization and Cellular Layers in a Gut-on-a-Chip System Using Optical Coherence Tomography Optical Coherence Tomography Measurements as Potential Imaging Biomarkers for Parkinson's Disease: A Systematic Review and Meta-analysis