1. Articles from Javier A. Jo

    1-16 of 16
    1. Methylene blue-filled biodegradable polymer particles as a contrast agent for optical coherence tomography

      Methylene blue-filled biodegradable polymer particles as a contrast agent for optical coherence tomography

      Optical coherence tomography (OCT) images largely lack molecular information or molecular contrast. We address that issue here, reporting on the development of biodegradable micro and nano-spheres loaded with methylene blue (MB) as molecular contrast agents for OCT. MB is a constituent of FDA approved therapies and widely used as a dye in off-label clinical applications. The sequestration of MB within the polymer reduced toxicity and improved signal strength by drastically reducing the production of singlet oxygen and leuco-MB. The former leads to tissue damage and the latter to reduced image contrast. The spheres are also strongly scattering which improves molecular ...

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    2. Intravascular optical coherence tomography method for automated detection of macrophage infiltration within atherosclerotic coronary plaques

      Intravascular optical coherence tomography method for automated detection of macrophage infiltration within atherosclerotic coronary plaques

      Background and aims Significant macrophages infiltration in advanced atherosclerotic plaques promotes acute coronary events. Hence, the clinical imaging of macrophage content in coronary atherosclerotic plaques could potentially aid in identifying patients most at risk of future acute coronary events. The aim of this study was to introduce and validate a simple intravascular optical coherence tomography (IV-OCT) image processing method for automated, accurate and fast detection of macrophage infiltration within coronary atherosclerotic plaques. Methods This method calculates the ratio of the normalized-intensity standard deviation (NSD) values estimated over two axially-adjacent regions of interest in an IV-OCT cross-sectional image (B-scan). When applied ...

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      Mentions: Javier A. Jo
    3. AI-Assisted In Situ Detection of Human Glioma Infiltration Using a Novel Computational Method for Optical Coherence Tomography

      AI-Assisted In Situ Detection of Human Glioma Infiltration Using a Novel Computational Method for Optical Coherence Tomography

      Purpose: In glioma surgery, it is critical to maximize tumor resection without compromising adjacent non-cancerous brain tissue. Optical Coherence Tomography (OCT) is a non-invasive, label-free, real-time, high-resolution imaging modality that has been explored for glioma infiltration detection. Here we report a novel artificial intelligence (AI) assisted method for automated, real-time, in situ detection of glioma infiltration at high spatial resolution. Experimental Design: Volumetric OCT datasets were intraoperatively obtained from resected brain tissue specimens of 21 patients with glioma tumors of different stages and labeled as either non-cancerous or glioma-infiltrated based on histopathology evaluation of the tissue specimens (gold standard). Labeled ...

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    4. Multiple Research Scientist and Postdoctoral Positions in Computer Aided Diagnosis Department of Biomedical Engineering, Texas A&M University, College Station-Texas

      Multiple Research Scientist and Postdoctoral Positions in Computer Aided Diagnosis Department of Biomedical Engineering, Texas A&M University, College Station-Texas

      The Laboratory for Optical Diagnosis and Imaging ( http://biomed.tamu.edu/LODI ) in the Department of Biomedical Engineering at Texas A&M University invites applications for multiple research scientist and postdoctoral positions in the area of computer aided diagnosis (CAD) system development. Ideal candidates should have substantial experience in 2D and 3D pattern recognition, computer vision, image analysis, and machine learning. Experience in both deep learning and medical imaging analysis is highly desirable. Successful applicants will join a dynamic, multidisciplinary and international team focused on developing and clinically validating endoscopy imaging based CAD tools for automated in-situ cancer detection. Excellent ...

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    5. Multiple Research Scientist and Postdoctoral Positions in Endoscopic Instrumentation Department of Biomedical Engineering, Texas A&M University, College Station-Texas

      Multiple Research Scientist and Postdoctoral Positions in Endoscopic Instrumentation Department of Biomedical Engineering, Texas A&M University, College Station-Texas

      The Laboratory for Optical Diagnosis and Imaging ( http://biomed.tamu.edu/LODI ) in the Department of Biomedical Engineering at Texas A&M University invites applications for multiple research scientist and postdoctoral positions in the area of endoscopic instrumentation development. Ideal candidates should have expertise in optical design, data acquisition instrumentation and software development, and substantial demonstrated experience in optical imaging and/or spectroscopic instrumentation design and implementation. Experience in fluorescence lifetime imaging microscopy (FLIM) and/or optical coherence tomography (OCT) is highly desirable. Successful applicants will join a dynamic, multidisciplinary and international team focused on developing and clinically validating optical ...

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    6. Contrast enhancement of pump-probe optical coherence tomography (PP-OCT) based molecular imaging using methylene blue loaded PLGA particles

      Contrast enhancement of pump-probe optical coherence tomography (PP-OCT) based molecular imaging using methylene blue loaded PLGA particles

      Atherosclerosis, a condition in which plaque accumulates on the inner wall of arteries, is often recognized as a precursor to cardiovascular diseases (CVDs), the most common causes of death in the US. Optical Coherence Tomography (OCT) is an intravascular optical diagnosis tool, which can be used to obtain high resolution morphological images of atherosclerotic plaque. However, atherosclerotic plaque components, such as macrophages, can be misclassified due to their signal similarities to fibrin accumulations, cholesterol crystals and microcalcifications. To overcome these challenges, we develop a biocompatible contrast agent to enhance molecular imaging of a Pump-Probe OCT (PPOCT) system. Methylene blue (MB ...

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    7. Automatic classification of atherosclerotic plaques imaged with intravascular OCT

      Automatic classification of atherosclerotic plaques imaged with intravascular OCT

      Intravascular optical coherence tomography (IV-OCT) allows evaluation of atherosclerotic plaques; however, plaque characterization is performed by visual assessment and requires a trained expert for interpretation of the large data sets. Here, we present a novel computational method for automated IV-OCT plaque characterization. This method is based on the modeling of each A-line of an IV-OCT data set as a linear combination of a number of depth profiles. After estimating these depth profiles by means of an alternating least square optimization strategy, they are automatically classified to predefined tissue types based on their morphological characteristics. The performance of our proposed method ...

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    8. Multimodal optical coherence tomography and fluorescence lifetime imaging with interleaved excitation sources for simultaneous endogenous and exogenous fluorescence

      Multimodal optical coherence tomography and fluorescence lifetime imaging with interleaved excitation sources for simultaneous endogenous and exogenous fluorescence

      Multimodal imaging probes a variety of tissue properties in a single image acquisition by merging complimentary imaging technologies. Exploiting synergies amongst the data, algorithms can be developed that lead to better tissue characterization than could be accomplished by the constituent imaging modalities taken alone. The combination of optical coherence tomography (OCT) with fluorescence lifetime imaging microscopy (FLIM) provides access to detailed tissue morphology and local biochemistry. The optical system described here merges 1310 nm swept-source OCT with time-domain FLIM having excitation at 355 and 532 nm. The pulses from 355 and 532 nm lasers have been interleaved to enable simultaneous ...

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    9. Automated analysis of multimodal fluorescence lifetime imaging and optical coherence tomography data for the diagnosis of oral cancer in the hamster cheek pouch model

      Automated analysis of multimodal fluorescence lifetime imaging and optical coherence tomography data for the diagnosis of oral cancer in the hamster cheek pouch model

      It is known that the progression of oral cancer is accompanied by changes in both tissue biochemistry and morphology. A multimodal imaging approach combining functional and structural imaging modalities could therefore provide a more comprehensive prognosis of oral cancer. This idea forms the central theme of the current study, wherein this premise is examined in the context of a multimodal imaging system that combines fluorescence lifetime imaging (FLIM) and optical coherence tomography (OCT). Towards this end, in the first part of the present study, the diagnostic advantage obtained by using both fluorescence intensity and lifetime information is assessed. In the ...

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    10. Simultaneous morphological and biochemical endogenous optical imaging of atherosclerosis

      Simultaneous morphological and biochemical endogenous optical imaging of atherosclerosis

      Aims The aim of this study was to validate novel imaging technology for simultaneous morphological and biochemical endogenous optical imaging of coronary atherosclerotic plaque. Methods and results Optical coherence tomography (OCT) generates high-resolution 3D images of plaque morphology and endogenous fluorescence lifetime imaging microscopy (FLIM) characterizes biochemical composition. Both imaging modalities rely on plaque's intrinsic optical characteristics, making contrast agents unnecessary. A multimodal OCT/FLIM system was utilized to generate luminal biochemical maps superimposed on high-resolution (7 µm axial and 13 µm lateral) structural volumetric images. Forty-seven fresh postmortem human coronary segments were imaged: pathological intimal thickening (PIT, n ...

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    11. Feature of the Week 10/19/14: Automated Classification of Optical Coherence Tomography Images for the Diagnosis of Oral Malignancy in the Hamster Cheek Pouch

      Feature of the Week 10/19/14: Automated Classification of Optical Coherence Tomography Images for the Diagnosis of Oral Malignancy in the Hamster Cheek Pouch

      Most studies evaluating the potential of optical coherence tomography (OCT) for the diagnosis of oral cancer are based on visual assessment of OCT B-scans by trained experts. Human interpretation of the large pool of data acquired by modern high-speed OCT systems, however, can be cumbersome and extremely time consuming. Development of image analysis methods for automated and quantitative OCT image analysis could therefore facilitate the evaluation of such a large volume of data. We report automated algorithms for quantifying structural features that are associated with the malignant transformation of the oral epithelium based on image processing of OCT data. The ...

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    12. Automated classification of optical coherence tomography images for the diagnosis of oral malignancy in the hamster cheek pouch

      Automated classification of optical coherence tomography images for the diagnosis of oral malignancy in the hamster cheek pouch

      Most studies evaluating the potential of optical coherence tomography (OCT) for the diagnosis of oral cancer are based on visual assessment of OCT B-scans by trained experts. Human interpretation of the large pool of data acquired by modern high-speed OCT systems, however, can be cumbersome and extremely time consuming. Development of image analysis methods for automated and quantitative OCT image analysis could therefore facilitate the evaluation of such a large volume of data. We report automated algorithms for quantifying structural features that are associated with the malignant transformation of the oral epithelium based on image processing of OCT data. The ...

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    13. In Vivo Simultaneous Morphological and Biochemical Optical Imaging of Oral Epithelial Cancer

      In Vivo Simultaneous Morphological and Biochemical Optical Imaging of Oral Epithelial Cancer

      Early detection of cancer is key to reducing morbidity and mortality. Morphological and chemical biomarkers presage the transition from normal to cancerous tissue. We have developed a noninvasive imaging system incorporating optical coherence tomography (OCT) and fluorescence lifetime imaging microscopy (FLIM) into a single optical system for the first time, in order to acquire both sets of biomarkers. OCT can provide morphological images of tissue with high resolution, while FLIM can provide biochemical tissue maps. Coregistered OCT volumes and FLIM images have been acquired simultaneously in an in vivo hamster cheek pouch model of oral cancer. The OCT images indicate ...

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    14. A dual-modality optical coherence tomography and fluorescence lifetime imaging microscopy system for simultaneous morphological and biochemical tissue characterization

      A dual-modality optical coherence tomography and fluorescence lifetime imaging microscopy system for simultaneous morphological and biochemical tissue characterization

      Most pathological conditions elicit changes in the tissue optical response that may be interrogated by one or more optical imaging modalities. Any single modality typically only furnishes an incomplete picture of the tissue optical response, hence an approach that integrates complementary optical imaging modalities is needed for a more comprehensive non-destructive and minimally-invasive tissue characterization. We have developed a dual-modality system, incorporating optical coherence tomography (OCT) and fluorescence lifetime imaging microscopy (FLIM), that is capable of simultaneously characterizing the 3-D tissue morphology and its biochemical composition. The Fourier domain OCT subsystem, at an 830 nm center wavelength, provided high-resolution morphological ...

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    15. Multimodality optical imaging combining optical coherence tomography (OCT) and fluorescence lifetime imaging (FLIM) for morphological and biochemical tissue characterization

      Multimodality optical imaging combining optical coherence tomography (OCT) and fluorescence lifetime imaging (FLIM) for morphological and biochemical tissue characterization

      Here we report on our current efforts to simultaneously quantify both morphological and biochemical tissue information by combining optical coherence tomography (OCT) and fluorescence lifetime imaging (FLIM). The Fourier domain OCT module is built around a custom designed high-speed spectrometer (bandwidth of 102 nm, 3 dB rolloff of 1.2 mm, lines rates of up to 59 kHz). A 40 nm bandwidth SLED centered at 830 nm provided an axial resolution of 7.6 mm for OCT. The objective lens provided 10 um lateral resolution for OCT and 100 um for FLIM. Lateral OCT and FLIM beam scanning was accomplished ...

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    16. Morphological and biochemical imaging of coronary atherosclerotic plaques using optical coherence tomography and fluorescence lifetime imaging

      We propose that a multimodality imaging approach combining high-resolution optical coherence tomography (OCT) with fluorescence lifetime imaging microscopy (FLIM) molecular imaging will allow a more accurate diagnosis of vulnerable plaque (VP).We have obtained consecutive OCT and FLIM images of two postmortem coronary arteries. In the first artery (left), the OCT images showed a homogeneous, signal-rich, thick upper layer that suggested a homogenous fibrotic plaque. The FLIM images indicated two biochemically distinct areas: region of interest (ROI)-1 resembled the fluorescence emission of collagen, while ROI-2 resembled the fluorescence emission of elastin. In the second artery (right), the OCT images ...
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    1-16 of 16
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  2. Topics in the News

    1. (16 articles) Javier A. Jo
    2. (14 articles) Texas A&M University
    3. (11 articles) Brian E. Applegate
    4. (7 articles) Paritosh Pande
    5. (3 articles) FDA
    6. (2 articles) University of São Paulo
    7. (2 articles) Baylor College of Medicine
    8. (2 articles) Wihan Kim
    9. (1 articles) Jiefeng Xi
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    11. (1 articles) Tufts University
    12. (1 articles) UCLA
    13. (1 articles) Massachusetts General Hospital
    14. (1 articles) Capital Medical University
    15. (1 articles) University of Auckland
    16. (1 articles) Frederique Vanholsbeeck
    17. (1 articles) Francesco Bandello
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    Multimodality optical imaging combining optical coherence tomography (OCT) and fluorescence lifetime imaging (FLIM) for morphological and biochemical tissue characterization A dual-modality optical coherence tomography and fluorescence lifetime imaging microscopy system for simultaneous morphological and biochemical tissue characterization In Vivo Simultaneous Morphological and Biochemical Optical Imaging of Oral Epithelial Cancer Automated classification of optical coherence tomography images for the diagnosis of oral malignancy in the hamster cheek pouch Feature of the Week 10/19/14: Automated Classification of Optical Coherence Tomography Images for the Diagnosis of Oral Malignancy in the Hamster Cheek Pouch Simultaneous morphological and biochemical endogenous optical imaging of atherosclerosis Automated analysis of multimodal fluorescence lifetime imaging and optical coherence tomography data for the diagnosis of oral cancer in the hamster cheek pouch model Automatic classification of atherosclerotic plaques imaged with intravascular OCT Multiple Research Scientist and Postdoctoral Positions in Endoscopic Instrumentation Department of Biomedical Engineering, Texas A&M University, College Station-Texas Predictors for Rapid Progression of Coronary Calcification: An Optical Coherence Tomography Study Macular neovascularization in AMD, CSC and best vitelliform macular dystrophy: quantitative OCTA detects distinct clinical entities Effects of the Gaze Fixation Position on AS-OCT Measurements of the Limbus and Extraocular Muscle Insertion Site Distance