1. Articles from Guillermo J. Tearney

    1-24 of 160 1 2 3 4 5 6 7 »
    1. Co-cultured microfluidic model of the airway optimized for microscopy and micro-optical coherence tomography imaging

      Co-cultured microfluidic model of the airway optimized for microscopy and micro-optical coherence tomography imaging

      We have developed a human bronchial epithelial (HBE) cell and endothelial cell co-cultured microfluidic model to mimic the in vivo human airway. This airway-on-a-chip was designed with a central epithelial channel and two flanking endothelial channels, with a three-dimensional monolayers of cells growing along the four walls of the channel, forming central clear lumens. These cultures mimic airways and microvasculature in vivo . The central channel cells are grown at air-liquid interface and show features of airway differentiation including tight-junction formation, mucus production, and ciliated cells. Combined with novel micro-optical coherence tomography, this chip enables functional imaging of the interior of ...

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    2. Intranasal micro-optical coherence tomography imaging for cystic fibrosis studies

      Intranasal micro-optical coherence tomography imaging for cystic fibrosis studies

      Cystic fibrosis (CF) is a genetic disease caused by mutations in the CF transmembrane conductance regulator (CFTR) gene. Although impairment of mucociliary clearance contributes to severe morbidity and mortality in people with CF, a clear understanding of the pathophysiology is lacking. This is, in part, due to the absence of clinical imaging techniques capable of capturing CFTR-dependent functional metrics at the cellular level. Here, we report the clinical translation of a 1-μm resolution micro-optical coherence tomography (μOCT) technology to quantitatively characterize the functional microanatomy of human upper airways. Using a minimally invasive intranasal imaging approach, we performed a clinical ...

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    3. Imaging the Human Prostate Gland Using 1-μm-Resolution Optical Coherence Tomography

      Imaging the Human Prostate Gland Using 1-μm-Resolution Optical Coherence Tomography

      Context.— The accuracy of needle biopsy for the detection of prostate cancer is limited by well-known sampling errors. Thus, there is an unmet need for a microscopic screening tool that can screen large regions of the prostate comprehensively for cancer. Previous prostate imaging by optical coherence tomography (OCT) has had insufficient resolution for imaging cellular features related to prostate cancer. We have recently developed micro-optical coherence tomography (μOCT) that generates depth-resolved tissue images at a high frame rate with an isotropic resolution of 1 μm. Objective.— To demonstrate that optical images obtained with μOCT provide cellular-level contrast in prostate specimens ...

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    4. Plaque burden can be assessed using intravascular optical coherence tomography and a dedicated automated processing algorithm: a comparison study with intravascular ultrasound

      Plaque burden can be assessed using intravascular optical coherence tomography and a dedicated automated processing algorithm: a comparison study with intravascular ultrasound

      Aims Plaque burden (PB) measurement using intravascular optical coherence tomography (IVOCT) is currently thought to be inferior to intravascular ultrasound (IVUS). We developed an automated IVOCT image processing algorithm to enhance the external elastic lamina (EEL) contour. Thus, we investigated the accuracies of standard IVOCT and an IVOCT enhancement algorithm for measuring PB using IVUS as the reference standard. Methods and results The EEL-enhancement algorithm combined adaptive attenuation compensation, exponentiation, angular registration, and image averaging using three sequential frames. In two different laboratories with intravascular imaging expertise, PB was quantified on 200 randomized, matched IVOCT and IVUS images by four ...

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    5. Methods, systems, arrangements and computer-accessible medium for providing micro-optical coherence tomography procedures

      Methods, systems, arrangements and computer-accessible medium for providing micro-optical coherence tomography procedures

      Exemplary apparatus and method can be provided for obtaining data regarding a plurality of samples. For example, using at least one arrangement, it is possible to receive interferometric information that is based on radiations provided from a reference and the samples that are provided in respective chambers. Alternatively and/or in addition, based on the interferometric information, it is possible to discriminate between agents to identify a particular agent that affects a particular function within at least one of the samples.

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    6. Imaging the Human Prostate Gland Using 1-μm-Resolution Optical Coherence Tomography

      Imaging the Human Prostate Gland Using 1-μm-Resolution Optical Coherence Tomography

      Context.— The accuracy of needle biopsy for the detection of prostate cancer is limited by well-known sampling errors. Thus, there is an unmet need for a microscopic screening tool that can screen large regions of the prostate comprehensively for cancer. Previous prostate imaging by optical coherence tomography (OCT) have had insufficient resolution for imaging cellular features related to prostate cancer. We have recently developed micro-optical coherence tomography (μOCT) that generates depth-resolved tissue images at a high frame rate with an isotropic resolution of 1 μm. Objective.— To demonstrate that optical images obtained with μOCT provide cellular-level contrast in prostate specimens ...

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    7. In Vivo and Ex Vivo Microscopy: Moving Toward the Integration of Optical Imaging Technologies Into Pathology Practice

      In Vivo and Ex Vivo Microscopy: Moving Toward the Integration of Optical Imaging Technologies Into Pathology Practice

      The traditional surgical pathology assessment requires tissue to be removed from the patient, then processed, sectioned, stained, and interpreted by a pathologist using a light microscope. Today, an array of alternate optical imaging technologies allow tissue to be viewed at high resolution, in real time, without the need for processing, fixation, freezing, or staining. Optical imaging can be done in living patients without tissue removal, termed in vivo microscopy, or also in freshly excised tissue, termed ex vivo microscopy. Both in vivo and ex vivo microscopy have tremendous potential for clinical impact in a wide variety of applications. However, in ...

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    8. Intravascular optical coherence tomography

      Intravascular optical coherence tomography

      Intravascular optical coherence tomography (IVOCT) began at MIT in the early 1990’s with the recognition that OCT could be used for clinical applications other than ophthalmology. Initial steps made there towards IVOCT feasibility included the acquisition of cadaver arterial images demonstrating the capacity of OCT to visualize depth-resolved architectural detail of plaque 5 and the development of the first mechanical scanning catheter. 6 Early histopathologic correlation studies from cadaver arteries demonstrated that IVOCT was capable of distinguishing the major fibrous, fibrocalcific, and lipid-rich plaque types and also potentially identifying inflammation as manifested by macrophage accumulations. 7, 8 Data from ...

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    9. Visualizing cellular markers of sensorineural hearing loss in the murine cochlea using micro-optical coherence tomography

      Visualizing cellular markers of sensorineural hearing loss in the murine cochlea using micro-optical coherence tomography

      Although sensorineural hearing loss (SNHL) affects 600 million people globally and its prevalence is increasing, therapies remain limited. Advances in therapy development for SNHL are slow because we do not possess a method for visualizing the cochlea’s interior in living humans and relating visualized pathology to a patient’s hearing ability. To this end, we are investigating the ability of micro-optical coherence tomography (µOCT), a low-coherence interferometric imaging technique that requires no contrast agent and improves upon standard OCT in resolution and depth of focus, to visualize the micron-sized cellular structures in the cochlea. We recently demonstrated µOCT’s ...

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    10. Optical coherence tomography-guided laser marking with tethered capsule endomicroscopy

      Optical coherence tomography-guided laser marking with tethered capsule endomicroscopy

      Tethered capsule endomicroscopy (TCE) is a new method for performing comprehensive microstructural OCT imaging of gastrointestinal (GI) tract in unsedated patients in a well-tolerated and cost-effective manner. These features of TCE bestow it with significant potential to improve the screening, surveillance and management of various upper gastrointestinal diseases. To achieve clinical adoption of this imaging technique, it is important to validate it with co-registered histology, the current diagnostic gold standard. One such method for co-registering OCT images with histology is laser cautery marking, previously demonstrated using a balloon-centering OCT catheter that operates in conjunction with sedated endoscopy. With laser marking ...

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    11. Contrast enhancement of microscopic birefringent crystals using polarization sensitive micro-optical coherence tomography

      Contrast enhancement of microscopic birefringent crystals using polarization sensitive micro-optical coherence tomography

      Background: Birefringent crystals such as cholesterol and monosodium urate have recently been identified as possible pharmacologic targets for the treatment of coronary artery disease. The size of these crystals can be very small (on the order of 1 µm), making them difficult to identify. To image these microscopic crystals and enhance contrast, we modified existing micro optical coherence tomography system so that it was capable of obtaining polarization-sensitive images (PS-µOCT). A spectrometer-based PS-µOCT system was developed using a 270 nm wide broadband light source centered at 765 nm. Light was polarized using a polarizer and coupled to a ...

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    12. Cellular-resolution, extended depth of focus optical coherence tomography catheter toward in vivo cardiovascular imaging

      Cellular-resolution, extended depth of focus optical coherence tomography catheter toward in vivo cardiovascular imaging

      Optical coherence tomography (OCT) has been a useful clinical tool for diagnosing coronary artery disease through a flexible catheter, but its full promise relies on resolving cellular and sub-cellular structures in vivo. Previously, visualizing cellular structures through an imaging catheter is not possible due to limited depth of focus (DOF) of a tightly focused Gaussian beam: typically, a Gaussian beam with 2-3 μm resolution has a DOF within 100 μm, which is not sufficient for in vivo catheter imaging. Therefore, we developed a self-imaging wavefront division optical system that generates a coaxially-focused multimode (CAFM) beam with a DOF that is ...

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    13. Automated detection of esophageal dysplasia in in vivo optical coherence tomography images of the human esophagus

      Automated detection of esophageal dysplasia in in vivo optical coherence tomography images of the human esophagus

      Catheter-based Optical Coherence Tomography (OCT) devices allow real-time and comprehensive imaging of the human esophagus. Hence, they provide the potential to overcome some of the limitations of endoscopy and biopsy, allowing earlier diagnosis and better prognosis for esophageal adenocarcinoma patients. However, the large number of images produced during every scan makes manual evaluation of the data exceedingly difficult. In this study, we propose a fully automated tissue characterization algorithm, capable of discriminating normal tissue from Barrett’s Esophagus (BE) and dysplasia through entire three-dimensional (3D) data sets, acquired in vivo. The method is based on both the estimation of the ...

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    14. Clinical Translation of Tethered Confocal Microscopy Capsule for Unsedated Diagnosis of Eosinophilic Esophagitis

      Clinical Translation of Tethered Confocal Microscopy Capsule for Unsedated Diagnosis of Eosinophilic Esophagitis

      Esophagogastroduodenoscopy (EGD) is a widely used procedure, posing significant financial burden on both healthcare systems and patients. Moreover, EGD is time consuming, sometimes difficult to tolerate, and suffers from an imperfect diagnostic yield as the limited number of collected biopsies does not represent the whole organ. In this paper, we report on technological and clinical feasibility of a swallowable tethered endomicroscopy capsule, which is administered without sedation, to image large regions of esophageal and gastric mucosa at the cellular level. To demonstrate imaging capabilities, we conducted a human pilot study (n = 17) on Eosinophilic Esophagitis (EoE) patients and healthy volunteers ...

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    15. Extended depth of focus for coherence-based cellular imaging

      Extended depth of focus for coherence-based cellular imaging

      Improving lateral resolution for cross-sectional optical coherence tomography (OCT) imaging is difficult due to the rapid divergence of light once it is focused to a small spot. To overcome this obstacle, we introduce a fiber optics system that generates a coaxially focused multimode (CAFM) beam for depth of focus (DOF) extension. We fabricated a CAFM beam OCT probe and show that the DOF is more than fivefold that of a conventional Gaussian beam, enabling cross-sectional imaging of biological tissues with clearly resolved cellular and subcellular structures over more than a 400 μm depth range. The compact and straightforward design and ...

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    16. Assessment of acquired mucociliary clearance defects using micro-optical coherence tomography

      Assessment of acquired mucociliary clearance defects using micro-optical coherence tomography

      Background Dehydration of airway surface liquid (ASL) disrupts normal mucociliary clearance (MCC) in sinonasal epithelium, which may lead to chronic rhinosinusitis (CRS). Abnormal chloride (Cl − ) transport is one such mechanism that contributes to this disorder and can be acquired secondary to environmental perturbations, such as hypoxia at the tissue surface. The objective of this study was to assess the technological feasibility of the novel micro-optical coherence tomography (μOCT) imaging technique for investigating acquired MCC defects in cultured human sinonasal epithelial (HSNE) cells. Methods Primary HSNE cell cultures were subjected to a 1% oxygen environment for 12 hours to induce acquired ...

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    17. Methods and systems for performing angle-resolved Fourier-domain optical coherence tomography

      Methods and systems for performing angle-resolved Fourier-domain optical coherence tomography

      Arrangements, apparatus and methods are provided according to exemplary embodiments of the present invention. In particular, at least one first electro-magnetic radiation may be received and at least one second electro-magnetic radiation within a solid angle may be forwarded to a sample. The second electro-magnetic radiation may be associated with the first electro-magnetic radiation. A plurality of third electro-magnetic radiations can be received from the sample which is associated with the second electro-magnetic radiation, and at least one portion of the third electro-magnetic radiation is provided outside a periphery of the solid angle. Signals associated with each of the third ...

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    18. Imaging system, method and distal attachment for multidirectional field of view endoscopy

      Imaging system, method and distal attachment for multidirectional field of view endoscopy

      An exemplary apparatus for imaging at least one anatomical structure can be provided. For example, the apparatus can include an endoscopic first arrangement, a radiation source second arrangement which provides at least one electro-magnetic radiation, and a third arrangement attached to at least one portion of the endoscopic arrangement. The third arrangement can contain an optical arrangement which, upon impact by the at least one electro-magnetic radiation and based thereon, may transmit a first radiation and reflects a second radiation. The first radiation can impact at least one first portion of the anatomical structure(s), and the second radiation can ...

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    19. Endoscopic optical coherence tomography: technologies and clinical applications [Invited]

      Endoscopic optical coherence tomography: technologies and clinical applications [Invited]

      In this paper, we review the current state of technology development and clinical applications of endoscopic optical coherence tomography (OCT). Key design and engineering considerations are discussed for most OCT endoscopes, including side-viewing and forward-viewing probes, along with different scanning mechanisms (proximal-scanning versus distal-scanning). Multi-modal endoscopes that integrate OCT with other imaging modalities are also discussed. The review of clinical applications of endoscopic OCT focuses heavily on diagnosis of diseases and guidance of interventions. Representative applications in several organ systems are presented, such as in the cardiovascular, digestive, respiratory, and reproductive systems. A brief outlook of the field of endoscopic ...

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    20. Endoscopic biopsy apparatus, system and method

      Endoscopic biopsy apparatus, system and method

      Exemplary embodiments of apparatus, method and system for determining a position on or in a biological tissue can be provided. For example, using such exemplary embodiment, it is possible to control the focus of an optical imaging probe. In another exemplary embodiment, it is possible to implement a marking apparatus together with or into an optical imaging probe. According to one exemplary embodiment, it is possible (using one or more arrangements) to receive information associated with at least one image of at least one portion of the biological tissue obtained using an optical imaging technique. Further, it is possible to ...

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    21. Illuminating dynamic neutrophil trans-epithelial migration with micro-optical coherence tomography

      Illuminating dynamic neutrophil trans-epithelial migration with micro-optical coherence tomography

      A model of neutrophil migration across epithelia is desirable to interrogate the underlying mechanisms of neutrophilic breach of mucosal barriers. A co-culture system consisting of a polarized mucosal epithelium and human neutrophils can provide a versatile model of trans-epithelial migration in vitro , but observations are typically limited to quantification of migrated neutrophils by myeloperoxidase correlation, a destructive assay that precludes direct longitudinal study. Our laboratory has recently developed a new isotropic 1-μm resolution optical imaging technique termed micro-optical coherence tomography (μOCT) that enables 4D (x,y,z,t) visualization of neutrophils in the co-culture environment. By applying μOCT to ...

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    22. Assessment of ciliary phenotype in primary ciliary dyskinesia by micro-optical coherence tomography

      Assessment of ciliary phenotype in primary ciliary dyskinesia by micro-optical coherence tomography

      Ciliary motion defects cause defective mucociliary transport (MCT) in primary ciliary dyskinesia (PCD). Current diagnostic tests do not assess how MCT is affected by perturbation of ciliary motion. In this study, we sought to use micro-optical coherence tomography (μOCT) to delineate the mechanistic basis of cilia motion defects of PCD genes by functional categorization of cilia motion. Tracheae from three PCD mouse models were analyzed using μOCT to characterize ciliary motion and measure MCT. We developed multiple measures of ciliary activity, integrated these measures, and quantified dyskinesia by the angular range of the cilia effective stroke (ARC). Ccdc39 –/– mice, with ...

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    23. Postdoctoral Research Fellowships in Optical Coherence Tomography at Massachusetts General Hosptial

      Postdoctoral Research Fellowships in Optical Coherence Tomography at Massachusetts General Hosptial

      Employer Description Until recently, visualizing the architectural and cellular morphology of human tissue has required histopathological examination. Samples would be excised from the patient, processed, sectioned, stained and viewed under a microscope. In addition to being invasive, time consuming and costly, the static nature of conventional pathology prohibits the study of biological dynamics and function. The Tearney Laboratory at Massachusetts General Hospital has led the way in transforming the current diagnostic paradigm through the invention and translation of new noninvasive, high-resolution optical imaging modalities that enable disease diagnosis from living patients without excising tissues from the body. Led by Guillermo ...

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    1-24 of 160 1 2 3 4 5 6 7 »
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    1. (157 articles) Guillermo J. Tearney
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    3. (152 articles) Harvard University
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    Handbook of Optical Coherence Tomography (Book) Systems and methods for generating data based on one or more spectrally-encoded endoscopy techniques Heart wall velocimetry and exogenous contrast-based cardiac flow imaging in Drosophila melanogaster using Doppler optical coherence tomography Automated segmentation and characterization of esophageal wall in vivo by tethered capsule optical coherence tomography endomicroscopy Feature of the Week 04/24/2016: μOCT Imaging Using Depth of Focus Extension by Self-Imaging Wavefront Division in a Common-Path Fiber Optic Probe Postdoctoral Research Fellowships in Optical Coherence Tomography at Massachusetts General Hosptial Optical Coherence Tomography Angiography in Myopic Patients Quantification of retinal microvasculature and neurodegeneration changes in branch retinal vein occlusion after resolution of cystoid macular edema on optical coherence tomography angiography Machining head for a laser machining device Quantitative Comparison Of Microvascular Metrics On Three Optical Coherence Tomography Angiography Devices In Chorioretinal Disease Detection of and validation of shadows in intravascular images Automated intravascular plaque classification