1. Articles from chien-chung tsai

    1-14 of 14
    1. Rapid pseudo-H&E imaging using a fluorescence-inbuilt optical coherence microscopic imaging system

      Rapid pseudo-H&E imaging using a fluorescence-inbuilt optical coherence microscopic imaging system

      A technique using Linnik-based optical coherence microscopy (OCM), with built-in fluorescence microscopy (FM), is demonstrated here to describe cellular-level morphology for fresh porcine and biobank tissue specimens. The proposed method utilizes color-coding to generate digital pseudo-H&E (p-H&E) images. Using the same camera, colocalized FM images are merged with corresponding morphological OCM images using a 24-bit RGB composition process to generate position-matched p-H&E images. From receipt of dissected fresh tissue piece to generation of stitched images, the total processing time is <15 min for a 1-cm 2 specimen, which is on average two times faster than frozen-section H ...

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    2. Three-dimensional optical coherence tomography apparatus and its application

      Three-dimensional optical coherence tomography apparatus and its application

      Provided herein are devices and systems that apply full-field optical coherence tomography (OCT) technology to three-dimensional skin tissue imaging. A special designed Mirau type objective and an optical microscope module allowing both OCT mode and orthogonal polarization spectral imaging (OPSI) mode are disclosed.

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    3. Feature of The Week 03/26.2017: Segmentation of nucleus and cytoplasm of a single cell in 3D tomogram using OCT

      Feature of The Week 03/26.2017: Segmentation of nucleus and cytoplasm of a single cell in 3D tomogram using OCT

      uclear-to-cytoplasmic (N/C) ratio is an effective feature to distinguish cancer cells from their normal counterparts. In conventional cytopathology, cells printed or smeared on a glass slide were stained and then evaluated by two-dimensional (2D) images through light microscopy. A random rayburst sampling (RRBS) framework was developed to detect the nucleus and cell membrane boundaries in three-dimensional (3D) space. Raw images were acquired through a full-field optical coherence tomography (FF-OCT) system with submicron resolution—i.e. 0.8 µm in lateral and 0.9 µm in axial direction. The near isometric resolution enables 3D segmentation of nucleus and cell membrane ...

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    4. Segmentation of nucleus and cytoplasm of a single cell in three-dimensional tomogram using optical coherence tomography

      Segmentation of nucleus and cytoplasm of a single cell in three-dimensional tomogram using optical coherence tomography

      A random rayburst sampling (RRBS) framework was developed to detect the nucleus and cell membrane boundaries in three-dimensional (3-D) space. Raw images were acquired through a full-field optical coherence tomography system with submicron resolution—i.e., 0.8    μ m 0.8  μm in lateral and 0.9    μ m 0.9  μm in axial directions. The near-isometric resolution enables 3-D segmentation of a nucleus and cell membrane for determining the volumetric nuclear-to-cytoplasmic (N/C) ratio of a single cell. The RRBS framework was insensitive to the selection of seeds and image pixel noise. The robustness of the RRBS framework was verified ...

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    5. Feature Of The Week 9/28/14: National Taiwan University Reports on Full-depth epidermis tomography using a Mirau-based full-field OCT

      Feature Of The Week 9/28/14: National Taiwan University Reports on Full-depth epidermis tomography using a Mirau-based full-field OCT

      All living things are made of cells. Most living organisms are single cells; others, such as human bodies, are vast multicellular cities with an aggregation of more than 10 billion cells. Non-invasive and high speed imaging of cells and tissues with sub-micron resolution could help unveil functions of living organisms, and facilitate disease/cancer diagnosis, new drug development, etc. Such an imaging modality is one of the key technologies to detect disease and cancer in the early stage so that the ever-escalating medical cost could be controlled. At the same time, biomedical discovery often originated from technology advancement. Optical coherence ...

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    6. Full-depth epidermis tomography using a Mirau-based full-field optical coherence tomography

      Full-depth epidermis tomography using a Mirau-based full-field optical coherence tomography

      With a Gaussian-like broadband light source from high brightness Ce 3+ :YAG single-clad crystal fiber, a full-field optical coherence tomography using a home-designed Mirau objective realized high quality images of in vivo and excised skin tissues. With a 40 × silicone-oil-immersion Mirau objective, the achieved spatial resolutions in axial and lateral directions were 0.9 and 0.51 μm, respectively. Such a high spatial resolution enables the separation of lamellar structure of the full epidermis in both the cross-sectional and en face planes. The number of layers of stratum corneum and its thickness were quantitatively measured. This label free and non-invasive ...

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    7. Three-dimensional optical coherence tomography confocal imaging apparatus

      Three-dimensional optical coherence tomography confocal imaging apparatus

      A 3D OCT confocal imaging apparatus includes a light source module for providing an illumination beam with wider bandwidth from a crystal fiber; a reference source module; a pickup module; a beam splitter; an optical filter; and a sensor module. When the illumination beam illuminates a sample, a pickup objective lens and a piezoelectric actuator of the pickup module together provide an image beam scanning the sample in depth direction. The image beam and a reference beam from the reference source module together form an interference image beam, which is converted by a photosensor into a coherence image electric signal ...

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    8. Optical imaging apparatus and method

      Optical imaging apparatus and method

      The present invention relates to an optical imaging apparatus and a method, and more particularly to an optical imaging apparatus and a method with short coherence length optical source. The apparatus comprises an optical source with a plurality of outputs for providing a reference light and a sample light; a sample probe module for leading the sample light to a sample, and leading an information light out; an interference module for leading the reference light to a photo detector, and leading the information light to the photo detector; and a signal processing unit electrically coupled to the photo detector; wherein ...

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    9. Mirau-based full-field time-domain optical coherence tomography using Ce3+ :YAG crystal fiber light source

      Mirau-based full-field time-domain optical coherence tomography using Ce3+ :YAG crystal fiber light source

      Based on single-objective construction utilizing high brightness Ce 3+ :YAG single-clad crystal fiber light source, this Mirau-based full-field time-domain optical coherence tomography with circular polarization incident light represents deeper penetration in scattering medium. Using objective-changeable ability of home-designed Mirau objective, this system provides different applications, like biological tissue and single cells, by different spatial resolution with corresponding dynamics. High quality image relying on less ghost image and near common-path interference was demonstrated under this compact and power-stable system.

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    10. Non-invasive characterization of the domain boundary and structure properties of periodically poled ferroelectrics

      Non-invasive characterization of the domain boundary and structure properties of periodically poled ferroelectrics
      Shaping the ferroelectric domains as waveguide, grating, lens, and prism are key to the successful penetration of periodically-poled ferroelectrics on various wavelength conversion applications. The complicated structures are, however, difficult to be fully characterized, especially the unexpected index contrast at the anti-parallel domain boundaries are typical in the order of 10−4 or less. An ultrahigh resolution optical coherence tomography was employed to fully characterize the domain boundary and structure properties of a periodically-poled lithium niobate (PPLN) waveguide with an axial resolution of 0.68 μm, an transversal resolution of 3.2 μm, and an index contrast sensitivity of 4x10 ...
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    11. Microstructural and microspectral characterization of a vertically aligned liquid crystal display panel

      Microstructural and microspectral characterization of a vertically aligned liquid crystal display panel
      e microstructural and microspectral characteristics of a vertically aligned liquid crystal display (VA-LCD) panel were obtained noninvasively for the first time. With 1 μm axial and 2 μm transversal resolutions, the cell gap profile beneath the patterned thin-film transistor of the VA-LCD panel can clearly be resolved. The thicknesses of the multiple thin-film layers and the embedded defects can also be unveiled. As far as spectral response is concerned, the light transmittance at the layer boundaries can be estimated from the measured reflectance, which is crucial information for the design of a highly transmissive panel. The color shift of the ...
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    12. High-Luminance White-Light Point Source Using Ce,Sm : YAG Double-Clad Crystal Fiber

      High-Luminance White-Light Point Source Using Ce,Sm : YAG Double-Clad Crystal Fiber
      Ce and Sm ions codoped yttrium aluminum garnet (Ce,Sm : YAG) double-clad crystal fibers (DCFs) were successfully fabricated using the codrawing laser-heated pedestal growth method. At a pump wavelength of 446 nm, white-light point source was produced from a 10-$mu{hbox {m}}$-core DCF. The amplified spontaneous emissions and the residual pump resulted in a directional high brightness point source. A luminance of $4.5times 10 ^{9}~hbox{cd}/hbox{m} ^{2}$ was generated. The luminous flux of the point white light was 6.2 lm. When coupled to a 200-$mu{hbox {m}}$-core multimode fiber, the output ...
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    13. Ce^3+:YAG double-clad crystal-fiber-based optical coherence tomography on fish cornea

      Ce^3+:YAG double-clad crystal-fiber-based optical coherence tomography on fish cornea
      A Ce3+:YAG double-clad crystal fiber (DCF) visible emission was used as the light source for optical coherence tomography (OCT). The visible emission was produced from a 10 μm core DCF pumped by a diode laser. The broadband emission and short central wavelength of this light source enabled the realization of 1.5 μm axial resolution in air. The relatively clean spectrum reduced the side lobe of its point-spread function, and therefore facilitated the generation of a high-quality image with less crosstalk between adjacent image pixels. As a demonstration, an Aplocheilus lineatus goldfish was experimented on to map out the ...
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    1-14 of 14
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    1. (14 articles) National Taiwan University
    2. (1 articles) AcuSolutions Inc
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    Ce^3+:YAG double-clad crystal-fiber-based optical coherence tomography on fish cornea High-Luminance White-Light Point Source Using Ce,Sm : YAG Double-Clad Crystal Fiber Microstructural and microspectral characterization of a vertically aligned liquid crystal display panel Non-invasive characterization of the domain boundary and structure properties of periodically poled ferroelectrics Mirau-based full-field time-domain optical coherence tomography using Ce3+ :YAG crystal fiber light source Optical imaging apparatus and method Full-depth epidermis tomography using a Mirau-based full-field optical coherence tomography Feature Of The Week 9/28/14: National Taiwan University Reports on Full-depth epidermis tomography using a Mirau-based full-field OCT Feature of The Week 03/26.2017: Segmentation of nucleus and cytoplasm of a single cell in 3D tomogram using OCT Three-dimensional opto-thermo-mechanical model for predicting photo-thermal optical coherence tomography responses in multilayer geometries The role of optical coherence tomography angiography in moderate and advanced primary open-angle glaucoma OCT-angiography follow-up of choroidal neovascularization treated with treat- and- extend aflibercept regimen to avoid over-treatment