1. Articles from Peter A. Netland

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    1. Visible-Light Optical Coherence Tomography Fibergraphy for Quantitative Imaging of Retinal Ganglion Cell Axon Bundles

      Visible-Light Optical Coherence Tomography Fibergraphy for Quantitative Imaging of Retinal Ganglion Cell Axon Bundles

      Purpose : To develop a practical technique for visualizing and quantifying retinal ganglion cell (RGC) axon bundles in vivo. Methods : We applied visible-light optical coherence tomography (vis-OCT) to image the RGC axon bundles, referred to as vis-OCT fibergraphy, of healthy wild-type C57BL/6 mice. After vis-OCT imaging, retinas were flat-mounted, immunostained with anti-beta-III tubulin (Tuj1) antibody for RGC axons, and imaged with confocal microscopy. We quantitatively compared the RGC axon bundle networks imaged by in vivo vis-OCT and ex vivo confocal microscopy using semi-log Sholl analysis. Results : Side-by-side comparison of ex vivo confocal microscopy and in vivo vis-OCT confirmed that vis-OCT ...

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    1. (1 articles) Northwestern University
    2. (1 articles) Hao F. Zhang
    3. (1 articles) Roman V. Kuranov
    4. (1 articles) Sun Yat-Sen University
    5. (1 articles) National Institutes of Health
    6. (1 articles) University of Illinois at Urbana-Champaign
    7. (1 articles) Tianjin University
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    11. (1 articles) Jun Zhang
    12. (1 articles) Robert Ritch
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