1. Articles from Pietro Rubegni

    1-19 of 19
    1. Comparison of reflectance confocal microscopy and line-field optical coherence tomography for the identification of keratinocyte skin tumours

      Comparison of reflectance confocal microscopy and line-field optical coherence tomography for the identification of keratinocyte skin tumours

      Background: Reflectance confocal microscopy (RCM) and line-field confocal optical coherence tomography (LC-OCT) are non-invasive imaging devices that can help in the clinical diagnosis of actinic keratosis (AK) and cutaneous squamous cell carcinoma (SCC). No studies are available on the comparison between these two technologies for the identification of the different features of keratinocyte skin tumours. Objectives: To compare RCM and LC-OCT findings in AK and SCC. Methods: A retrospective multicenter study was conducted. Tumours were imaged with RCM and LC-OCT devices before surgery, and the diagnosis was confirmed by histological examinations. LC-OCT and RCM criteria for AK/SCC were identified ...

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    2. Morphologic evaluation of melanocytic lesions with three-dimensional line-field confocal optical coherence tomography: correlation with histopathology and reflectance confocal microscopy. A pilot study

      Morphologic evaluation of melanocytic lesions with three-dimensional line-field confocal optical coherence tomography: correlation with histopathology and reflectance confocal microscopy. A pilot study

      Background Line-field confocal optical coherence tomography (LC-OCT) is a new in vivo emerging technique which provides cellular resolution, imaging depth (400 μm) and produces real-time images in both the horizontal and vertical plane and in 3 dimensions (3D). No previous description of different subtypes of melanocytic lesions and their correlation with histopathology and reflectance confocal microscopy has been reported. Objectives We aimed to describe the features of melanocytic lesions by LC-OCT and their correlation with histopathology and reflectance confocal microscopy (RCM). Methods Selected melanocytic benign lesions and melanomas were imaged in vivo with RCM and LC-OCT at the Hospital Clinic ...

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    3. Line-field confocal optical coherence tomography (LC-OCT) as a new tool for non-invasive differential diagnosis of pustular skin disorders

      Line-field confocal optical coherence tomography (LC-OCT) as a new tool for non-invasive differential diagnosis of pustular skin disorders

      Background: The spectrum of pustular skin disorders (PSD) is large and particularly challenging, including inflammatory, infectious and amicrobial diseases. Moreover, although pustules represent the unifying clinical feature, they can be absent or not fully developed in the early stage of the disease. The line-field confocal optical coherence tomography (LC-OCT) is a recently developed imaging technique able to perform a non-invasive, in vivo, examination of the epidermis and upper dermis, reaching very high image resolution and virtual histology. Objectives: We aimed to investigate the potentialities of LC-OCT in the non-invasive differential diagnosis of a series of 11 PSD with different etiology ...

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    4. Acquired digital fibrokeratoma: First observation by high-resolution skin ultrasound and line-field confocal optical coherence tomography

      Acquired digital fibrokeratoma: First observation by high-resolution skin ultrasound and line-field confocal optical coherence tomography

      High-resolution skin ultrasound was performed in Siena University Hospital (Italy) with a 10–22 MHz linear probe (MyLab Twice-Esaote biomedica®, Firenze, Italy) up to 3mm depth. 3 Examination of the base [ Figure 1 ] revealed an oval, homogeneously hyperechogenic structure within the epidermal layer (E), surrounded by two hyperechoic parallel bands due to the hyperkeratotic collarette, with a well-defined posterior shadow cone reaching the papillary dermis and reticular dermis [ Figure 2 ]. The lesion was totally excised for histopathologic examination, confirming the suspicion of acquired digital fibrokeratoma [ Figures 5 - 7 ]. The clinical-pathological classification proposed by Kint et al . includes a type I ...

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    5. Non-invasive scoring of cellular atypia in keratinocyte cancers in 3D LC-OCT images using Deep Learning

      Non-invasive scoring of cellular atypia in keratinocyte cancers in 3D LC-OCT images using Deep Learning

      Diagnosis based on histopathology for skin cancer detection is today's gold standard and relies on the presence or absence of biomarkers and cellular atypia. However it suffers drawbacks: it requires a strong expertise and is time-consuming. Moreover the notion of atypia or dysplasia of the visible cells used for diagnosis is very subjective, with poor inter-rater agreement reported in the literature. Lastly, histology requires a biopsy which is an invasive procedure and only captures a small sample of the lesion, which is insufficient in the context of large fields of cancerization. Here we demonstrate that the notion of cellular ...

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    6. Line-field confocal optical coherence tomography as a tool for three-dimensional in vivo quantification of healthy epidermis: a pilot study

      Line-field confocal optical coherence tomography as a tool for three-dimensional in vivo quantification of healthy epidermis: a pilot study

      Epidermal three-dimensional (3D) topography/quantification have not been completely characterized yet. The recently developed line-field confocal optical coherence tomography (LC-OCT) provides real-time, high-resolution, in-vivo 3D imaging of the skin. This pilot study aimed at quantifying epidermal metrics [epidermal thicknesses, dermal-epidermal junction (DEJ) undulation and keratinocyte number/shape/size] using 3D LC-OCT. For each study participant (8 female, skin-type-II, younger/older volunteers), seven body sites were imaged with LC-OCT. Epidermal metrics were calculated by segmentations and measurements assisted by artificial intelligence (AI) when appropriate. Thicknesses of epidermis/SC, DEJ undulation and keratinocyte nuclei volume varied across body sites. Evidence of keratinocyte ...

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    7. Line-field confocal optical coherence tomography of actinic keratosis: a case series

      Line-field confocal optical coherence tomography of actinic keratosis: a case series

      Line-field confocal optical coherence tomography (LC-OCT) is a non-invasive imaging technique that was recently developed, combining high penetration depth (up to 500-µm, approaching that of conventional OCT) with high isotropic resolution [lateral: 1.3 µm; axial: 1.1 µm - similar to that of reflectance confocal microscopy (RCM)]. 1 .

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    8. Line-field confocal optical coherence tomography of sebaceous hyperplasia: a case series

      Line-field confocal optical coherence tomography of sebaceous hyperplasia: a case series

      Line-field confocal optical coherence tomography (LC-OCT) is a newly developed non-invasive imaging technique that combines high penetration depth (500 µm, similar to conventional OCT) with high isotropic resolution (lateral 1.3 µm, axial 1.1 µm, similar to reflectance confocal microscopy [RCM]). 1 Moreover, with its ability to produce images in both vertical and horizontal planes, it enables a tridimensional (3D) analysis of the skin, which can prove very useful for assessing physiological 2 as well as pathological skin.

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    9. Delayed Tattoo Reaction From Red Dye With Overlapping Clinicopathological Features: Examination With High-Frequency Ultrasound and Line-Field Optical Coherence Tomography

      Delayed Tattoo Reaction From Red Dye With Overlapping Clinicopathological Features: Examination With High-Frequency Ultrasound and Line-Field Optical Coherence Tomography

      Introduction Delayed tattoo reactions include a wide range of clinical presentations and overlapping forms; thus diagnosis can be challenging. We present a case of a delayed tattoo reaction from red dye. Case Presentation An otherwise healthy 40-year-old man presented with firm elevated plaques that arose 4 months previously, within a multicolored tattoo on the right forearm, 8 months after the execution of the tattoo ( Figure 1A ). The patient claimed minimal and only occasional itching. Polarized dermoscopy (Luminis Visiomed, Caliber) highlighted multiple keratotic plugs within the lesion, limited to the red-dyed areas ( Figure 1 ). Examination with line-field confocal optical coherence tomography ...

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    10. Technology in Practical Dermatology (Textbook)

      Technology in Practical Dermatology (Textbook)

      This book provides a complete overview on the latest available technologies in dermatology, while discussing future trends of this ever-growing field. This handy guide provides clinicians and researchers with a clear understanding of the advantages and challenges of laser and imaging technologies in skin medicine today. It also includes a section on imaging techniques for the evaluation of skin tumors, with chapters devoted to dermoscopy, in vivo and ex vivo reflectance confocal microscopy, high frequency ultrasound, optical coherence tomography, and a closing part on latest approaches to wound management. Completed by over 200 clinical images, Current Technology in Practical Dermatology ...

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    11. Line-field confocal optical coherence tomography for high-resolution noninvasive imaging of skin tumors

      Line-field confocal optical coherence tomography for high-resolution noninvasive imaging of skin tumors

      An optical technique called line-field confocal optical coherence tomography (LC-OCT) is introduced for high-resolution, noninvasive imaging of human skin in vivo . LC-OCT combines the principles of time-domain optical coherence tomography and confocal microscopy with line illumination and detection using a broadband laser and a line-scan camera. LC-OCT measures the echo-time delay and amplitude of light backscattered from cutaneous microstructures through low-coherence interferometry associated with confocal spatial filtering. Multiple A-scans are acquired simultaneously while dynamically adjusting the focus. The resulting cross-sectional B-scan image is produced in real time at 10  frame  /  s. With an isotropic spatial resolution of ∼1  μm, the ...

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    1-19 of 19
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    Line-field confocal optical coherence tomography for high-resolution noninvasive imaging of skin tumors Examination of circumscribed palmar hypokeratosis with line-field confocal optical coherence tomography: Dermoscopic, ultrasonographic and histopathologic correlates Technology in Practical Dermatology (Textbook) Kaposi sarcoma of the glans: New findings by line field confocal optical coherence tomography examination Delayed Tattoo Reaction From Red Dye With Overlapping Clinicopathological Features: Examination With High-Frequency Ultrasound and Line-Field Optical Coherence Tomography New findings in non‐invasive imaging of aquagenic keratoderma: Line‐field optical coherence tomography, dermoscopy and reflectance confocal microscopy Tridimensional skin imaging in aquagenic keratoderma: virtual histology by line‐field confocal optical coherence tomography Line-field confocal optical coherence tomography of sebaceous hyperplasia: a case series Line-field confocal optical coherence tomography of actinic keratosis: a case series Line-field confocal optical coherence tomography as a tool for three-dimensional in vivo quantification of healthy epidermis: a pilot study Association of retinal thickness and microvasculature with cognitive performance and brain volumes in elderly adults A Deep Learning Framework for the Detection and Quantification of Reticular Pseudodrusen and Drusen on Optical Coherence Tomography