1. Articles from Muktesh Mohan

    1-9 of 9
    1. Ex-vivo molecular imaging with Upconversion Nanoparticles (UCNPs) using Photo Thermal Optical Coherence Tomography (PTOCT)

      Ex-vivo molecular imaging with Upconversion Nanoparticles (UCNPs) using Photo Thermal Optical Coherence Tomography (PTOCT)

      We demonstrate the photothermal optical coherence tomography (PTOCT) system, with upconversion nanoparticles (UCNPs) as a molecular probe. We synthesize hydrophilic, biocompatible upconversion nanoparticles (UCNPs) using hydrothermal synthesis. We developed the PTOCT system along with the signal processing tool and applied this technique on animal tissue phantom for targeted imaging. The ‘lock-in detection’ of the amplitude modulated photothermal beam (980 nm), which used to excite the UCNPs was the backbone of the signal processing algorithm. The signal processing was further established in different aspects. As an application part, the diffusion dynamics of the UCNPs was performed inside the tissue to study ...

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    2. Real-time assessment and characterization of immobilized lipase onto a natural matrix and qualitative reaction kinetic studies using swept-source optical coherence tomography

      Real-time assessment and characterization of immobilized lipase onto a natural matrix and qualitative reaction kinetic studies using swept-source optical coherence tomography

      The current study presents a method based on Optical Coherence Tomography (OCT) for non-destructive, real-time analysis and portrayal of immobilization efficacy for lipase on a natural matrix namely, eggshell. Subsequently, qualitative biochemical reaction kinetics of immobilized lipase was also studied. Successful immobilization of lipase on eggshell was confirmed by the presence of a clear peak in ‘A’ scan of OCT image. From immobilization kinetics it is clearly observed that the thickness of the highest peak of the A-scan increases significantly and peak intensity saturated after 90 min of incubation. Hydrolysis of oil using immobilized lipase indicated that the release of ...

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      Mentions: Raju Poddar
    3. Synthesis of NaGdF4:Er3+/Yb3+ Upconversion Particles as Exogenous Contrast Agent for Swept-Source Optical Coherence Tomography: In vitro Animal Tissue Imaging

      Synthesis of NaGdF4:Er3+/Yb3+ Upconversion Particles as Exogenous Contrast Agent for Swept-Source Optical Coherence Tomography: In vitro Animal Tissue Imaging

      Swept-source optical coherence tomography (SSOCT) is a non-contact, non-invasive bioimaging tool that usesvarious probes to get high contrast images. Rare-earth based upconversion particles (UCPs) providea uniqueplatform to enhance the capabilities of bioimaging techniques. The UCPs can act as contrast enhancers for SSOCT with additional optical functionality and hence in this work upconversion emission optimized PVP/NaGdF4:Er3+/Yb3+UCPs were synthesized and then injected to chicken breast tissues to enhance the SSOCT images. As synthesized samples have shown good biocompatibility towards HEK293, HeLa cell lines and showed apparent enhancement in contrast to SSOCT images. Additionally, compared to the bare NaGdF4 ...

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      Mentions: Raju Poddar
    4. In-vivo analysis of iron-gold composite nanoparticles as potential exogenous contrast agents for Magnetomotive Optical Coherence Tomography (MMOCT)

      In-vivo analysis of iron-gold composite nanoparticles as potential exogenous contrast agents for Magnetomotive Optical Coherence Tomography (MMOCT)

      Magnetomotive optical coherence tomography (MMOCT) is a functional modification of conventional optical coherence tomography (OCT). It utilizes the nano-scale displacement embedded magnetic nanoparticles (MNPs) and subsequent amplitude and phase changes in OCT interferogram scattered from samples. We report a new robust method for ‘phase-lock-in’ detection of MMOCT signals and subsequent imaging. We validated this method utilizing iron-gold (Fe-Au) composite nanoparticles as an exogenous contrast agent for MMOCT. High contrast and specific background-subtracted images was generated for Fe-Au MNPs targeted samples. MMOCT with MNPs imaging systems was corroborated with tissue phantoms and in-vivo swiss albino mice model with modulating external magnetic ...

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    5. In Vitro Imaging of Animal Tissue with Upconversion Nanoparticles (UCNPs) as a Molecular Probing Agent Using Swept Source Optical Coherence Tomography (SSOCT)

      In Vitro Imaging of Animal Tissue with Upconversion Nanoparticles (UCNPs) as a Molecular Probing Agent Using Swept Source Optical Coherence Tomography (SSOCT)

      Purpose The aim of the current study is to demonstrate the potential of rare earth (RE) based upconversion nanoparticles (UCNPs) as molecular probing agents for non-invasive depth-resolved bioimaging technique, swept-source optical coherence tomography (SSOCT). Methods The Na + doped La 2 O 3 :Er 3+ /Yb 3+ UCNPs were synthesized using a solution combustion route with characterization for emission spectra and morphological study. A custom made SSOCT system was also incorporated for tissue imaging with an A-scan rate of 100 kHz/s, the central wavelength of 1060 nm, an axial and lateral resolution of ~ 4.5 µm and ~ 13 µm in ...

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      Mentions: Raju Poddar
    6. Efficacy of Spectroscopic Optical Coherence Tomography (sOCT) for instantaneous study of nitrilase enzyme kinetics

      Efficacy of Spectroscopic Optical Coherence Tomography (sOCT) for instantaneous study of nitrilase enzyme kinetics

      We present monitoring of nitrilase enzyme assay in real-time by means of Spectroscopic Optical Coherence Tomography (sOCT). Combination of principal component analysis (PCA) algorithm with Swept Source Optical Coherence Tomography (SSOCT), to extract the absorption spectra of the sample, termed as sOCT. Spectroscopic information of nitrilase enzyme obtained from sOCT is used to investigate dynamic enzyme kinetic reactions. Different principle components give complete information regarding the composition of the samples. Further, Fourier Transform Infrared Spectroscopy (FTIR) analysis has been also performed to validate the PCA absorption spectra. Samples were prepared in controlled condition and evaluated at a regular time interval ...

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    7. Towards characterization of bacterial colonies and biofilms: an approach based on swept source optical coherence tomography

      Towards characterization of bacterial colonies and biofilms: an approach based on swept source optical coherence tomography

      The continuous development of microorganism colonies may lead to the development of biofilms. The ability to observe bacterial layers in real time using Optical Coherence Tomography (OCT) can be beneficial for cross-sectional growth studies of bacterial biofilms. A new mathematical model has been developed and utilized to calculate the backscattering optical properties of sample and estimate the necessary parameters. The spectroscopic based analytical method has been successfully demonstrated for characterization of different bacterial colonies and biofilms. The results were verified with conventional ‘Gram staining’ method. The proposed analytical method has potential to monitor realtime growth kinetics of bacteria. The quantities ...

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      Mentions: Raju Poddar
    8. Investigation of Puccinia triticina contagion on wheat leaves using swept source optical coherence tomography

      Investigation of Puccinia triticina contagion on wheat leaves using swept source optical coherence tomography

      We present an investigation on applicability of swept-source optical coherence tomography (SSOCT) to examine the internal structures of plant leaves. This study helps to identify the growth and spread of the leaf rust disease in leaves of wheat plants, caused by Puccinia triticina . The epidermal cell layer and parenchyma cell layer of the acquired OCT images are correlated with the histological images to show distinctive leaf morphological boundaries. In the OCT images, the thickness of the parenchyma cell layer in healthy and infected samples are compared and analyzed. Accurate morphological and quantitative information are obtained with our developed system for ...

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      Mentions: Raju Poddar
    9. Zinc oxide nanoparticles (ZnONPs) as contrast agent for imaging of animal tissue using swept source optical coherence tomography (SSOCT)

      Zinc oxide nanoparticles (ZnONPs) as contrast agent for imaging of animal tissue using swept source optical coherence tomography (SSOCT)

      We present applicability of ZnO nanoparticles (ZnONPs) as a potential contrast agent for Optical Coherence Tomography (OCT) imaging. A 1064 nm laser source was used in a swept source configuration capable of a scanning rate of 100 kHz (100,000 A-lines/s). Swept Source Optical Coherence Tomography (SSOCT) is a variation of OCT, offering greater scan rates, higher resolution and signal-to-noise ratios. To enhance the contrast of an OCT frame, ZnO nanoparticles were used as an exogeneous contrast agent, which were fabricated using zinc acetic acid derivation and oxalic acid via precipitation. The particles were characterized using UV–vis Spectroscopy ...

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      Mentions: Raju Poddar
    1-9 of 9
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    1. (6 articles) Raju Poddar
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    Zinc oxide nanoparticles (ZnONPs) as contrast agent for imaging of animal tissue using swept source optical coherence tomography (SSOCT) Investigation of Puccinia triticina contagion on wheat leaves using swept source optical coherence tomography Towards characterization of bacterial colonies and biofilms: an approach based on swept source optical coherence tomography Efficacy of Spectroscopic Optical Coherence Tomography (sOCT) for instantaneous study of nitrilase enzyme kinetics In Vitro Imaging of Animal Tissue with Upconversion Nanoparticles (UCNPs) as a Molecular Probing Agent Using Swept Source Optical Coherence Tomography (SSOCT) In-vivo analysis of iron-gold composite nanoparticles as potential exogenous contrast agents for Magnetomotive Optical Coherence Tomography (MMOCT) Synthesis of NaGdF4:Er3+/Yb3+ Upconversion Particles as Exogenous Contrast Agent for Swept-Source Optical Coherence Tomography: In vitro Animal Tissue Imaging Real-time assessment and characterization of immobilized lipase onto a natural matrix and qualitative reaction kinetic studies using swept-source optical coherence tomography Ex-vivo molecular imaging with Upconversion Nanoparticles (UCNPs) using Photo Thermal Optical Coherence Tomography (PTOCT) OCT parameters of the optic nerve head and the retina as surrogate markers of brain volume in a normal population, a pilot study Choroidal vessel density in unilateral hyperopic amblyopia using en-face optical coherence tomography Association between food and nutrients intakes and coronary plaque vulnerability in patients with coronary heart disease: An optical coherence tomography study