1. Articles from A. Claude Boccara

    1-13 of 13
    1. En face coherence microscopy

      En face coherence microscopy

      En face coherence microscopy or flying spot or full field optical coherence tomography or microscopy (FF-OCT/FF-OCM) belongs to the OCT family because the sectioning ability is mostly linked to the source coherence length. In this article we will focus our attention on the advantages and the drawbacks of the following approaches: en face versus B scan tomography in terms of resolution, coherent versus incoherent illumination and influence of aberrations, and scanning versus full field imaging. We then show some examples to illustrate the diverse applications of en face coherent microscopy and show that endogenous or exogenous contrasts can add ...

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    2. Full-field spatially incoherent illumination interferometry: a spatial resolution almost insensitive to aberrations

      Full-field spatially incoherent illumination interferometry: a spatial resolution almost insensitive to aberrations

      We show that with spatially incoherent illumination, the point spread function (PSF) width/spatial resolution of an imaging interferometer like that used in full-field optical coherence tomography (OCT) is almost insensitive to aberrations. In these systems, aberrations mostly induce a reduction of the signal level that leads to a loss of the signal-to-noise ratio without broadening the system PSF. This is demonstrated by comparison with traditional scanning OCT and wide-field OCT with spatially coherent illuminations. Theoretical analysis and numerical calculation as well as experimental results are provided to show this specific merit of incoherent illumination in full-field OCT. To the ...

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    3. Dynamic full field optical coherence tomography: subcellular metabolic contrast revealed in tissues by interferometric signals temporal analysis

      Dynamic full field optical coherence tomography: subcellular metabolic contrast revealed in tissues by interferometric signals temporal analysis

      We developed a new endogenous approach to reveal subcellular metabolic contrast in fresh ex vivo tissues taking advantage of the time dependence of the full field optical coherence tomography interferometric signals. This method reveals signals linked with local activity of the endogenous scattering elements which can reveal cells where other OCT-based techniques fail or need exogenous contrast agents. We benefit from the micrometric transverse resolution of full field OCT to image intracellular features. We used this time dependence to identify different dynamics at the millisecond scale on a wide range of organs in normal or pathological conditions.

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    4. Dynamic full field optical coherence tomography: subcellular metabolic contrast revealed in tissues by temporal analysis of interferometric signals

      Dynamic full field optical coherence tomography: subcellular metabolic contrast revealed in tissues by temporal analysis of interferometric signals

      We developed a new endogenous approach to reveal subcellular metabolic contrast in fresh ex vivo tissues taking advantage of the time dependence of the full field optical coherence tomography interferometric signals. This method reveals signals linked with local activity of the endogenous scattering elements which can reveal cells where other imaging techniques fail or need exogenous contrast agents. We benefit from the micrometric transverse resolution of full field OCT to image intracellular features. We used this time dependence to identify different dynamics at the millisecond scale on a wide range of organs in normal or pathological conditions.

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    5. Fingerprint imaging from the inside of a finger with full-field optical coherence tomography

      Fingerprint imaging from the inside of a finger with full-field optical coherence tomography

      Imaging below fingertip surface might be a useful alternative to the traditional fingerprint sensing since the internal finger features are more reliable than the external ones. One of the most promising subsurface imaging technique is optical coherence tomography (OCT), which, however, has to acquire 3-D data even when a single en face image is required. This makes OCT inherently slow for en face imaging and produce unnecessary large data sets. Here we demonstrate that full-field optical coherence tomography (FF-OCT) can be used to produce en face images of sweat pores and internal fingerprints, which can be used for the identification ...

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    6. Detection of plasmonic nanoparticles with full field-OCT: optical and photothermal detection

      Detection of plasmonic nanoparticles with full field-OCT: optical and photothermal detection

      Detecting the signal backscattered by nanoparticles immersed in highly scattering media such as biological tissue remains a challenge. In this article we report on the use of Full Field OCT (FF-OCT) to slice in depth in phantoms and in tissues in order a) to selectively observe the particles through the backscattered light at suitable wavelengths, and b) to detect the effects of the time-dependent response to full field optical heating through the strong absorption cross-section of these plasmonic nanoparticles. The analysis of the thermal wave behavior leads to the localization of the heat sources even when FF-OCT signals cannot reach ...

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    7. En-face full-field optical coherence tomography for fast and efficient fingerprints acquisition

      En-face full-field optical coherence tomography for fast and efficient fingerprints acquisition

      Optical coherence tomography (OCT) has been recently proposed by a number of laboratories as a promising tool for fingerprints acquisitions and for fakes discrimination. Indeed OCT being a non-contact, non-destructive optical method that virtually sections the volume of biological tissues that strongly scatter light it appears obvious to use it for fingerprints. Nevertheless most of the OCT setups have to go through the long acquisition of a full 3D image to isolate an “en-face” image suitable for fingerprint analysis. A few “en-face” OCT approaches have been proposed that use either a complex 2D scanning setup and image processing, or a ...

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    8. From supersonic shear wave imaging to full-field optical coherence shear wave elastograph

      From supersonic shear wave imaging to full-field optical coherence shear wave elastograph

      Elasticity maps of tissue have proved to be particularly useful in providing complementary contrast to ultrasonic imaging, e.g., for cancer diagnosis at the millimeter scale. Optical coherence tomography (OCT) offers an endogenous contrast based on singly backscattered optical waves. Adding complementary contrast to OCT images by recording elasticity maps could also be valuable in improving OCT-based diagnosis at the microscopic scale. Static elastography has been successfully coupled with full-field OCT (FF-OCT) in order to realize both micrometer-scale sectioning and elasticity maps. Nevertheless, static elastography presents a number of drawbacks, mainly when stiffness quantification is required. Here, we describe the ...

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    9. 3D static elastography at the micrometer scale using Full Field OCT

      3D static elastography at the micrometer scale using Full Field OCT

      Full-Field OCT (FF-OCT) is able to image biological tissues in 3D with micrometer resolution. In this study we add elastographic contrast to the FF-OCT modality. By combining FF-OCT with elastography, we create a virtual palpation map at the micrometer scale. We present here a proof of concept on multi-layer phantoms and preliminary results on ex vivo biological samples such as porcine cornea, human breast tissues and rat heart. The 3D digital volume correlation that is used in connection with the 3D stack of images allows to access to the full 3D strain tensor and to reveal stiffness anisotropy.

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    10. Single myelin fiber imaging in living rodents without labeling by deep optical coherence microscopy

      Single myelin fiber imaging in living rodents without labeling by deep optical coherence microscopy
      Myelin sheath disruption is responsible for multiple neuropathies in the central and peripheral nervous system. Myelin imaging has thus become an important diagnosis tool. However, in vivo imaging has been limited to either low-resolution techniques unable to resolve individual fibers or to low-penetration imaging of single fibers, which cannot provide quantitative information about large volumes of tissue, as required for diagnostic purposes. Here, we perform myelin imaging without labeling and at micron-scale resolution with >300-μm penetration depth on living rodents. This was achieved with a prototype [termed deep optical coherence microscopy (deep-OCM)] of a high-numerical aperture infrared full-field optical coherence ...
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    11. In vivo and in situ cellular imaging full-field optical coherence tomography with a rigid endoscopic probe

      In vivo and in situ cellular imaging full-field optical coherence tomography with a rigid endoscopic probe
      Full-field OCT has proved to be a powerful high-resolution cellular imaging tool for biological tissues. However the standard bulk full-field OCT setup does not match the size requirements for most in situ and in vivo imaging applications. We adapted its principle into a rigid needle-like probe using two coupled interferometers and incoherent illumination: an external processing interferometer is used for in-depth scanning, while a distal common-path interferometer at the tip of the probe collects light backscattered from the tissue. Our experimental setup achieves an axial and transversal resolution in tissue of 1.8 µm and 3.5 µm respectively, for ...
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    12. Full-field OCT approaches clinical application

      Full-field OCT approaches clinical application
      Newly developed Cell OCT, an approach to full-field optical coherence tomography, has enabled researchers to image breast-cancer tissue with histology-like results. Full-field OCT is an en face (transverse), broadband interferometric approach to optical coherence tomography (OCT) that’s been in use for life-science research for more than a decade.1–3 The technique offers many advantages, including fast tissue imaging at the cellular level, which is why at ESPCI (Ecole Superieure Physique Chimie Industrielles; Paris, France) we call it “Cell OCT.” Recently, full-field Cell OCT proved able to virtually slice ablated breast tumors and lymph nodes with 1 µm isotropic ...
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    1-13 of 13
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  2. Topics in the News

    1. (13 articles) Claude Boccara
    2. (12 articles) ESPCI ParisTech
    3. (5 articles) LLTech
    4. (3 articles) Amir Nahas
    5. (3 articles) Fabrice Harms
    6. (2 articles) Mathias Fink
    7. (1 articles) Mickaël Tanter
    8. (1 articles) Thu-Mai Nguyen
    9. (1 articles) Jean-Marie Chassot
    10. (1 articles) Anne Latrive
    11. (1 articles) National University of Singapore
    12. (1 articles) National Institutes of Health
    13. (1 articles) University of Kent
    14. (1 articles) Singapore Eye Research Institute
    15. (1 articles) Imperial College London
    16. (1 articles) Tin Aung
    17. (1 articles) Mani Baskaran
    18. (1 articles) Adrian G. Podoleanu
    19. (1 articles) Carl Zeiss Meditec
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    Full-field OCT approaches clinical application In vivo and in situ cellular imaging full-field optical coherence tomography with a rigid endoscopic probe Single myelin fiber imaging in living rodents without labeling by deep optical coherence microscopy 3D static elastography at the micrometer scale using Full Field OCT From supersonic shear wave imaging to full-field optical coherence shear wave elastograph En-face full-field optical coherence tomography for fast and efficient fingerprints acquisition Detection of plasmonic nanoparticles with full field-OCT: optical and photothermal detection Dark-field full-field optical coherence tomography Fingerprint imaging from the inside of a finger with full-field optical coherence tomography Dynamic full field optical coherence tomography: subcellular metabolic contrast revealed in tissues by interferometric signals temporal analysis Research Assistant/Associate in Biophotonics and Endoscopic Imaging at Imperial College London Optical coherence tomography angiography microvascular findings in macular edema due to central and branch retinal vein occlusions