1. Articles from Eva Lankenau

    1-15 of 15
    1. Label-Free In Vivo Imaging of Corneal Lymphatic Vessels Using Microscopic Optical Coherence Tomography

      Label-Free In Vivo Imaging of Corneal Lymphatic Vessels Using Microscopic Optical Coherence Tomography

      Purpose : Corneal neovascularization, in particular lymphangiogenesis, is a limiting factor in corneal transplant survival. Novel treatment approaches focus on (selective) inhibition and regression of lymphatic vessels. Imaging clinically invisible corneal lymphatic vessels is a prerequisite for these strategies. Using a murine model, this study investigates whether corneal lymphatic vessels can be imaged using microscopic optical coherence tomography (mOCT). Methods : Corneal neovascularization was induced by intrastromal placement of 11.0 nylon sutures in one eye of BALB/c mice. After 2 weeks, cross-sectional images and volumes of the corneas with a 0.5 mm lateral and axial field of view were ...

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    2. Intraoperative assessment of laryngeal pathologies with optical coherence tomography integrated into a surgical microscope

      Intraoperative assessment of laryngeal pathologies with optical coherence tomography integrated into a surgical microscope

      Objective Endoscopic examination followed by tissue biopsy is the gold standard in the evaluation of lesions of the upper aerodigestive tract. However, it can be difficult to distinguish between healthy mucosa, dysplasia, and invasive carcinoma. Optical coherence tomography (OCT) is a non-invasive technique which acquires high-resolution, cross-sectional images of tissue in vivo . Integrated into a surgical microscope, it allows the intraoperative evaluation of lesions simultaneously with microscopic visualization. Study Design In a prospective case series, we evaluated the use of OCT integrated into a surgical microscope during microlaryngoscopy to help differentiating various laryngeal pathologies. Methods 33 patients with laryngeal pathologies ...

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    3. Detection of cervical intraepithelial neoplasia by using optical coherence tomography in combination with microscopy

      Detection of cervical intraepithelial neoplasia by using optical coherence tomography in combination with microscopy

      Optical coherence tomography (OCT) is a noninvasive high-resolution imaging technique that permits the detection of cancerous and precancerous lesions of the uterine cervix. The purpose of this study was to evaluate a new system that integrates an OCT device into a microscope. OCT images were taken from loop electrosurgical excision procedure (LEEP) specimens under microscopic guidance. The images were blinded with respect to their origin within the microscopic image and analyzed independently by two investigators using initially defined criteria and later compared to the corresponding histology. Sensitivity and specificity were calculated with respect to the correct identification of high-grade squamous ...

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    4. Advantages of microscope-integrated intraoperative online optical coherence tomography: usage in Boston keratoprosthesis type I surgery

      Advantages of microscope-integrated intraoperative online optical coherence tomography: usage in Boston keratoprosthesis type I surgery

      Boston keratoprosthesis (KPro) type I is a technique to treat patients with corneal diseases that are not amenable to conventional keratoplasty. Correct assembly and central implantation of the prosthesis are crucial for postoperative visual recovery. This study investigates the potential benefit of intraoperative optical coherence tomography (OCT) to monitor KPro surgery. Retrospective case series are presented for two patients who underwent Boston KPro type I implantation. The surgery in both patients was monitored intraoperatively using a commercially available intraoperative OCT (iOCT) device mounted on a surgical microscope. Microscope-integrated intraoperative OCT was able to evaluate the correct assembly and implantation of ...

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    5. Future Prospects: Assessment of Intraoperative Optical Coherence Tomography in Ab Interno Glaucoma Surgery

      Future Prospects: Assessment of Intraoperative Optical Coherence Tomography in Ab Interno Glaucoma Surgery

      Purpose : Ab interno glaucoma surgery , such as trabecular aspiration or ab interno trabeculotomy, has been introduced as an alternative, non-filtering procedure to lower intraocular pressure in glaucoma patients. The purpose of the present study was to assess the feasibility of intraoperative spectral domain optical coherence tomography (iOCT) for improving ab interno glaucoma surgery . Materials and Methods : Intraoperative optical coherence tomography was performed to visualize the anterior chamber angle, the aspiration canula during trabecular aspiration, and the trabectome device during ab interno trabeculotomy. A commercially available 840-nm iOCT was used in a clinical setting, a 1310-nm spectral-domain OCT in an experimental ...

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    6. Microanatomy of the tympanic membrane in chronic myringitis obtained with optical coherence tomography

      Microanatomy of the tympanic membrane in chronic myringitis obtained with optical coherence tomography

      A microscope-based optical coherence tomography (OCT) device was used to assess the microanatomy of the tympanic membrane in patients with chronic myringitis. A prospective study was designed for this purpose. OCT measurements of the tympanic membrane were done on 11 patients with myringitis with a microscope-based spectral domain OCT system. The in vivo findings were compared with those findings of a control group consisting of 36 patients with retraction pockets or atrophic tympanic membranes ( n = 13), myringosclerosis ( n = 12) and perforations ( n = 11). In active chronic myringitis, the thickness of the tympanic membrane is increased compared to healthy membranes and ...

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    7. Optimising deep anterior lamellar keratoplasty (DALK) using intraoperative online optical coherence tomography (iOCT)

      Optimising deep anterior lamellar keratoplasty (DALK) using intraoperative online optical coherence tomography (iOCT)

      Background/aims To describe the use of intraoperative online optical coherence tomography (iOCT) for improving deep anterior lamellar keratoplasty (DALK) surgery. Methods Retrospective case series of 6 eyes of 6 male patients with keratokonus, corneal dystrophy or herpetic stromal scars undergoing DALK were investigated using intraoperative optical coherence tomography and postsurgical image/video analysis. Main outcome measures were: visibility of surgical steps, especially, assessment of placement depth of injection needle, preparation of bare Descemet's membrane and drainage of interface fluid. Results iOCT enables real-time visualisation of all surgical steps of DALK procedure in all patients. Placement of air injection ...

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    8. Optimizing Descemet Membrane Endothelial Keratoplasty Using Intraoperative Optical Coherence Tomography

      Optimizing Descemet Membrane Endothelial Keratoplasty Using Intraoperative Optical Coherence Tomography

      Importance Descemet membrane endothelial keratoplasty (DMEK) is a challenging procedure for the surgeon, particularly because of deficient visibility of the delicate tissue due to the natural en face view through the operating microscope. A cross-sectional view would greatly enhance intraoperative overview and enable the surgeon to better control the procedure. Objective To retrospectively analyze the use of intraoperative optical coherence tomography (iOCT) for improving the safety of DMEK. Design Intraoperative OCT during DMEK was performed in 26 eyes of 26 patients. We retrospectively analyzed imaging and video data. Setting Department of Ophthalmology, University of Cologne. Participants Seven men and 19 ...

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    9. Real-time volumetric optical coherence tomography OCT imaging with a surgical microscope

      Real-time volumetric optical coherence tomography OCT imaging with a surgical microscope
      Optical coherence tomography is a unique technique to visualize subsurface tissue structures with a resolution below 10μm during microsurgery without tissue contact. Since it was introduced more than 15 years ago imaging speed was boosted by more than three orders of magnitude, from less than 100 to more than 300,000 A-scans per second. Instead of taking only still images of anatomical structures, the increased speed of OCT allows now to image volumes nearly in real time. This enables not only the scanning of larger tissue surfaces, but also opens new application beyond simple diagnosis. A noncontact volumetric imaging with ...
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    10. An optical coherence tomography study for imaging the round window niche and the promontorium tympani

      An optical coherence tomography study for imaging the round window niche and the promontorium tympani
      Tympanosclerosis may involve the tympanic membrane, the ossicles, as well as the oval and round window niche, respectively. The surgical treatment of the obliterated oval window niche is most challenging. Beside stapesplasty, vibroplasty with coupling the floating mass transducer (FMT) onto the round window niche and into a new, so-called third window is indicated. In the latter situation, drilling a hole into the promontorium is necessary to couple the FMT close to the membranous endosteum. Any damage of the membranous inner ear needs to be avoided. The question was whether OCT is useful to identify the endosteum and to provide ...
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    11. Optical coherence tomography allows for the reliable identification of laryngeal epithelial dysplasia and for precise biopsy: A clinicopathological study of 61 patients undergoing microlaryngoscopy

      Optical coherence tomography allows for the reliable identification of laryngeal epithelial dysplasia and for precise biopsy: A clinicopathological study of 61 patients undergoing microlaryngoscopy
      Objectives/Hypothesis: A newly developed microscope-based spectral-domain optical coherence tomography (SD-OCT) device and an endoscope-based time-domain OCT (TD-OCT) were used to assess the inter-rater reliability, sensitivity, specificity, and accuracy of benign and dysplastic laryngeal epithelial lesions. Study Design: Prospective study. Methods: OCT during microlaryngoscopy was done on 35 patients with an endoscope-based TD-OCT, and on 26 patients by an SD-OCT system integrated into an operating microscope. Biopsies were taken from microscopically suspicious lesions allowing comparative study of OCT images and histology. Results: Thickness of the epithelium was seen to be the main criterion for degree of dysplasia. The inter-rater reliability ...
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    12. Optical coherence tomography for process control of laser micromachining

      Optical coherence tomography for process control of laser micromachining
      In situ surface imaging for nondestructive evaluation (NDE) by optical coherence tomography (OCT) before, during, and after ablative laser processing is presented. Furthermore, it is shown that the ability of in situ characterization is beneficial for samples such as optical fibers, which are difficult to handle in the standard analysis. Surface images taken by the OCT are compared with these common analysis tools such as scanning electron microscopy (SEM), reflected-light, and confocal microscopy. An axial resolution of ∼ 126 nm for surface detection and a lateral resolution <2.5 μm are obtained and the potential of the setup to imaging structures ...
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    13. Optical coherence tomography with online visualization of more than seven rendered volumes per second

      Optical coherence tomography with online visualization of more than seven rendered volumes per second
      Nearly real-time visualization of 3-D volumes is crucial for the use of optical coherence tomography (OCT) during microsurgery. With an ultrahigh speed spectral domain OCT coupled to a surgical microscope, on-line display of 7.2 rendered volumes at 87 megapixels per second is demonstrated. Calculating the A-scans from the spectra is done on a quad-core personal computer (PC), while dedicated software for the 3-D rendering is executed on a high performance video card. Imaging speed is practically only limited by the readout of the camera. First experiments show the feasibility of real-time 3-D OCT for guided interventions.
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    14. 3-D intraoperative imaging combining an operating microscope with optical coherence tomography (OCT)

      Objective Optical coherence tomography (OCT) allows non-contact/non-invasive analysis of tissues with a penetration depth of 2–3 mm reaching a spatial resolution of approximately 4–15 μm. This resolution is compatible with the resolution of modern operation microscopes. Aim of this work was to provide a three-dimensional (3D) intraoperative visualization of tissue structures. Material and methods A high-speed spectral domain OCT (a modified version of the Ganymed, Thorlabs HL) was adapted to a motorized operation microscope (HR 1000, Möller-Wedel). Via a specially designed two axis-scanner, the 840 nm OCT was adapted directly to a camera port of the operation ...
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    15. Optical Coherence Tomography (OCT) – An Emerging Technology for Three-Dimensional Imaging of Biological Tissues

      Optical imaging technologies offer high resolution non-contact visualization of tissues with a large variety of contrast modalities. However tissues are optically inhomogeneous due to microscopic variations of the refractive index. The resulting scattering limits resolution and imaging depth. Optical coherence tomography (OCT) is a relatively new approach for obtaining 3-D high resolution imaging of tissue which is successfully used in the diagnosis of the eye diseases. However, OCT is not limited to medical applications. This review will cover theory and technology of OCT and provide an overview over current use and future prospects in tissue engineering. Content Type Book ChapterDOI ...
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    1-15 of 15
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  2. Topics in the News

    1. (12 articles) Gereon Hüttmann
    2. (10 articles) University of Lübeck
    3. (4 articles) Universität Rostock
    4. (4 articles) University of Cologne
    5. (4 articles) OPMedt
    6. (2 articles) Thorlabs
    7. (2 articles) Haag-Streit
    8. (1 articles) Ludwig Maximilian University of Munich
    9. (1 articles) Julia Gallwas
    10. (1 articles) Christian Dannecker
    11. (1 articles) ESPCI ParisTech
    12. (1 articles) Michalina J. Góra
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    Optical coherence tomography with online visualization of more than seven rendered volumes per second Optical coherence tomography for process control of laser micromachining Optical coherence tomography allows for the reliable identification of laryngeal epithelial dysplasia and for precise biopsy: A clinicopathological study of 61 patients undergoing microlaryngoscopy Real-time volumetric optical coherence tomography OCT imaging with a surgical microscope An optical coherence tomography study for imaging the round window niche and the promontorium tympani Optimizing Descemet Membrane Endothelial Keratoplasty Using Intraoperative Optical Coherence Tomography Optimising deep anterior lamellar keratoplasty (DALK) using intraoperative online optical coherence tomography (iOCT) Detection of cervical intraepithelial neoplasia by using optical coherence tomography in combination with microscopy Full-field optical coherence tomography: novel imaging technique for extemporaneous high-resolution analysis of mucosal architecture in human gut biopsies Simon Poole, Cylite co-founder, elected as Fellow of Australian Academy of Science Influence of neoatherosclerosis on prognosis and treatment response in patients with in-stent restenosis Tracking the invasion of breast cancer cells in paper-based 3D cultures by OCT motility analysis