1. Articles from Marie Seveno

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    1. Optical Coherence Tomography: Imaging Mouse Retinal Ganglion Cells In Vivo

      Optical Coherence Tomography: Imaging Mouse Retinal Ganglion Cells In Vivo

      Structural changes in the retina are common manifestations of ophthalmic diseases. Optical coherence tomography (OCT) enables their identification in vivo —rapidly, repetitively, and at a high resolution. This protocol describes OCT imaging in the mouse retina as a powerful tool to study optic neuropathies (OPN). The OCT system is an interferometry-based, non-invasive alternative to common post mortem histological assays. It provides a fast and accurate assessment of retinal thickness, allowing the possibility to track changes, such as retinal thinning or thickening. We present the imaging process and analysis with the example of the Opa1 delTTAG mouse line. Three types of ...

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