1. Articles from Hinnerk Schulz-Hildebrandt

    1-8 of 8
    1. Dynamic contrast in scanning microscopic OCT

      Dynamic contrast in scanning microscopic OCT

      While optical coherence tomography (OCT) provides a resolution down to 1 micrometer it has difficulties to visualize cellular structures due to a lack of scattering contrast. By evaluating signal fluctuations, a significant contrast enhancement was demonstrated using time-domain full-field OCT (FF-OCT), which makes cellular and subcellular structures visible. The putative cause of the dynamic OCT signal is ATP-dependent motion of cellular structures in a sub-micrometer range, which provides histology-like contrast. Here we demonstrate dynamic contrast with a scanning frequency-domain OCT (FD-OCT). Given the inherent sectional imaging geometry, scanning FD-OCT provides depth-resolved images across tissue layers, a perspective known from histopathology ...

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    2. Intravital microscopic optical coherence tomography imaging to assess mucus mobilizing interventions for muco-obstructive lung disease in mice

      Intravital microscopic optical coherence tomography imaging to assess mucus mobilizing interventions for muco-obstructive lung disease in mice

      Airway mucus obstruction is a hallmark of chronic lung diseases such as cystic fibrosis, asthma and COPD, and the development of more effective mucus mobilizing therapies remains an important unmet need for patients with these muco-obstructive lung diseases. However, methods for sensitive visualization and quantitative assessment of immediate effects of therapeutic interventions on mucus clearance in vivo are lacking. In this study, we determined if newly developed high-speed microscopic optical coherence tomography (mOCT) is sensitive to detect and compare in vivo effects of inhaled isotonic saline, hypertonic saline and bicarbonate on mucus mobilization and clearance in Scnn1b -transgenic mice with ...

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    3. Investigation of cell dynamics in 3D cell spheroids and cell interaction with 3D printed scaffolds by mOCT

      Investigation of cell dynamics in 3D cell spheroids and cell interaction with 3D printed scaffolds by mOCT

      Optical coherence tomography is a non-invasive and label-free imaging modality based on the detection of backscattered light in samples. As microscopic OCT (mOCT) combines high axial and lateral resolution, an investigation of biological and printed samples at subcellular level is feasible. The additional excellent depth resolution enables mOCT to be a suitable quality control of 3D printed samples. The use of speckle variance adds information about cell viability. Here we present the feasibility of investigating cell viability within a cell spheroid and monitoring the cell interaction with bioprinted scaffolds using mOCT.

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    4. Optimization-based vessel segmentation pipeline for robust quantification of capillary networks in skin with optical coherence tomography angiography

      Optimization-based vessel segmentation pipeline for robust quantification of capillary networks in skin with optical coherence tomography angiography

      Optical coherence tomography angiography (OCTA) provides in-vivo images of microvascular perfusion in high resolution. For its application to basic and clinical research, an automatic and robust quantification of the capillary architecture is mandatory. Only this makes it possible to reliably analyze large amounts of image data, to establish biomarkers, and to monitor disease developments. However, due to its optical properties, OCTA images of skin often suffer from a poor signal-to-noise ratio and contain imaging artifacts. Previous work on automatic vessel segmentation in OCTA mostly focuses on retinal and cerebral vasculature. Its applicability to skin and, furthermore, its robustness against imaging ...

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    5. High-speed fiber scanning endoscope for volumetric multi-megahertz optical coherence tomography

      High-speed fiber scanning endoscope for volumetric multi-megahertz optical coherence tomography

      We present a forward-viewing fiber scanning endoscope (FSE) for high-speed volumetric optical coherence tomography (OCT). The reduction in size of the probe was achieved by substituting the focusing optics by an all-fiber-based imaging system which consists of a combination of scanning single-mode fibers, a glass spacer, made from a step-index multi-mode fiber, and a gradient-index fiber. A lateral resolution of 11 μm was achieved at a working distance of 1.2 mm. The newly designed piezo-based FSE has an outer diameter of 1.6 mm and a rigid length of 13.5 mm. By moving the whole imaging optic in ...

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    6. Novel endoscope with increased depth of field for imaging human nasal tissue by microscopic optical coherence tomography

      Novel endoscope with increased depth of field for imaging human nasal tissue by microscopic optical coherence tomography

      Intravital microscopy (IVM) offers the opportunity to visualize static and dynamic changes of tissue on a cellular level. It is a valuable tool in research and may considerably improve clinical diagnosis. In contrast to confocal and non-linear microscopy, optical coherence tomography (OCT) with microscopic resolution (mOCT) provides intrinsically cross-sectional imaging. Changing focus position is not needed, which simplifies especially endoscopic imaging. For in-vivo imaging, here we are presenting endo-microscopic OCT (emOCT). A graded-index-lens (GRIN) based 2.75 mm outer diameter rigid endoscope is providing 1.5 – 2 µm nearly isotropic resolution over an extended field of depth. Spherical and chromatic ...

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    7. Label-Free In Vivo Imaging of Corneal Lymphatic Vessels Using Microscopic Optical Coherence Tomography

      Label-Free In Vivo Imaging of Corneal Lymphatic Vessels Using Microscopic Optical Coherence Tomography

      Purpose : Corneal neovascularization, in particular lymphangiogenesis, is a limiting factor in corneal transplant survival. Novel treatment approaches focus on (selective) inhibition and regression of lymphatic vessels. Imaging clinically invisible corneal lymphatic vessels is a prerequisite for these strategies. Using a murine model, this study investigates whether corneal lymphatic vessels can be imaged using microscopic optical coherence tomography (mOCT). Methods : Corneal neovascularization was induced by intrastromal placement of 11.0 nylon sutures in one eye of BALB/c mice. After 2 weeks, cross-sectional images and volumes of the corneas with a 0.5 mm lateral and axial field of view were ...

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    8. An Approach for Needle Based Optical Coherence Elastography Measurements

      An Approach for Needle Based Optical Coherence Elastography Measurements

      While navigation and interventional guidance are typically based on image data, the images do not necessarily reflect mechanical tissue properties. Optical coherence elastography (OCE) presents a modality with high sensitivity and very high spatial and temporal resolution. However, OCE has a limited field of view of only 2–5 mm depth. We present a side-facing needle probe to image externally induced shear waves from within soft tissue. A first method of quantitative needle-based OCE is provided. Using a time of flight setup, we establish the shear wave velocity and estimate the tissue elasticity. For comparison, an external scan head is ...

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    1-8 of 8
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  2. Topics in the News

    1. (8 articles) University of Lübeck
    2. (8 articles) Gereon Hüttmann
    3. (1 articles) Harvard University
    4. (1 articles) Massachusetts General Hospital
    5. (1 articles) University of Cologne
    6. (1 articles) Columbia University
    7. (1 articles) Robert A. Huber
    8. (1 articles) Tom Pfeiffer
    9. (1 articles) Reginald Birngruber
    10. (1 articles) Medical University of Vienna
    11. (1 articles) Harbin Medical University
    12. (1 articles) Université Pierre et Marie Curie
    13. (1 articles) University of Erlangen
    14. (1 articles) Wolfgang Drexler
    15. (1 articles) Adrian G. Podoleanu
    16. (1 articles) Mengyang Liu
    17. (1 articles) Bo Yu
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    An Approach for Needle Based Optical Coherence Elastography Measurements Label-Free In Vivo Imaging of Corneal Lymphatic Vessels Using Microscopic Optical Coherence Tomography Novel endoscope with increased depth of field for imaging human nasal tissue by microscopic optical coherence tomography High-speed fiber scanning endoscope for volumetric multi-megahertz optical coherence tomography Optimization-based vessel segmentation pipeline for robust quantification of capillary networks in skin with optical coherence tomography angiography Investigation of cell dynamics in 3D cell spheroids and cell interaction with 3D printed scaffolds by mOCT Intravital microscopic optical coherence tomography imaging to assess mucus mobilizing interventions for muco-obstructive lung disease in mice Dynamic contrast in scanning microscopic OCT Changes in the Retinal Microvasculature Measured Using Optical Coherence Tomography Angiography According to Age Direct en-face, speckle-reduced images using angular-compounded Master-Slave optical coherence tomography A case report of a recurrent early and late Bioresorbable vascular scaffold thrombosis: serial angiography and optical coherence tomography findings Optical coherence tomography angiography as a novel approach to contactless evaluation of sublingual microcirculation: A proof of principle study