1. Articles from Byeong Ha Lee

    1-24 of 55 1 2 3 »
    1. Angiographic Imaging of an In Vivo Mouse Brain as a Guiding Star for Automatic Digital Refocusing in OCT

      Angiographic Imaging of an In Vivo Mouse Brain as a Guiding Star for Automatic Digital Refocusing in OCT

      A method allows the extraction of the recovery factor that maximizes the image contrast of OCT (optical coherence tomography) and/or OCTA (OCT angiography) of a living subject is proposed in this study. Due to the finite depth of focus in imaging optics, the volume OCT imaging suffers from blurriness in the lateral resolution. By utilizing the digital hologram method or angular spectrum method, the blurred image can be refocused. However, for in vivo OCT imaging, evaluation of the image focus is not easy, owing to the cloudy structure of the brain. In the proposed method, the blood flow signals ...

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    2. Post-Processing Method for Image Reconstruction Enhancement in Integrating-Bucket-Based Full-Field Optical Coherence Tomography

      Post-Processing Method for Image Reconstruction Enhancement in Integrating-Bucket-Based Full-Field Optical Coherence Tomography

      An integrating-bucket method is widely used as a reconstruction tool for full-field optical coherence tomography (FF-OCT). However, it requires high-precision adjustments of the phase modulation parameters. If the parameters are not optimal, the reconstructed tomogram will incur severe artifacts. We propose a post-processing method for removing or reducing such artifacts by utilizing a correction factor extracted from a pre-reconstructed tomogram. FF-OCT imaging created using a coin verifies the effectiveness of the method not only for a single en face image but also for an entire 3-D image. It is expected that the proposed method will expand the application of FF-OCT ...

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    3. Identification of Fungus-infected Tomato Seeds Based on Full-Field Optical Coherence Tomography

      Identification of Fungus-infected Tomato Seeds Based on Full-Field Optical Coherence Tomography

      The morphological changes of anthracnose (fungus) -infected tomato seeds have been studied to identify the infection and characterize its effect. Full-field optical coherence tomography (FF-OCT) has been utilized as a nondestructive but efficient modality for visualizing the effects of fungal infection. The cross-sectional images extracted from a stack of en face FF-OCT images showed significant changes with infection in the seed structure. First of all, the seed coat disappeared with the infection. The thickness of the seed coat of a healthy seed was measured as 28.2 µm, with a standard deviation of 1.2 µm. However, for infected seeds ...

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    4. Quantitative discrimination of pearls using polarization-sensitive optical coherence tomography

      Quantitative discrimination of pearls using polarization-sensitive optical coherence tomography

      We propose a robust method that can quantitatively discriminate genuine pearls from imitation ones by introducing the concept of entropy in the polarization-sensitive optical coherence tomography (PS-OCT). Qualitatively, by examining the birefringence properties of the nacre region of pearls with PS-OCT, the genuine pearls can be easily discriminated. To quantify the amount of birefringence formation, however, the concept of phase retardation entropy is introduced, which is expected to have a higher value when a PS-OCT tomogram has more diverse phase retardation values in its histogram. Experimental confirmation demonstrated that the phase retardation entropy of a genuine pearl was always higher ...

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    5. In vivo three-dimensional imaging of human corneal nerves using Fourier-domain optical coherence tomography

      In vivo three-dimensional imaging of human corneal nerves using Fourier-domain optical coherence tomography

      We have employed Fourier-domain optical coherence tomography (FD-OCT) to achieve corneal nerve imaging, which could be useful in surgical planning and refractive surgery. Because the three-dimensional (3-D) images of the corneal nerves were acquired in vivo, unintentional movement of the subject during the measurement led to imaging artifacts. These artifacts were compensated for with a series of signal processing techniques, namely realigning A-scan images to flatten the boundary and cross-correlating adjacent B-scan images. To overcome the undesirably large signal from scattering at the corneal surface and iris, volume rendering and maximum intensity projections were performed with only the data taken ...

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    6. Mesenchymal stem cell-laden hybrid scaffold for regenerating subacute tympanic membrane perforation

      Mesenchymal stem cell-laden hybrid scaffold for regenerating subacute tympanic membrane perforation

      ympanic membrane (TM) perforation is one of the most common otology complications. To date, there has not been reported TM regeneration using bioprinted scaffold. The purpose of this study was to evaluate the efficacy and feasibility of bioprinted polycaprolactone/collagen/alginate-mesenchymal stem cell (PCAMSC) scaffolds for the regeneration of subacute TM perforation. Sprague-Dawley rats were used in an animal model of subacute TM perforation. In the experimental group (n = 7), bioprinted 3D PCAMSC scaffold was placed on the perforation. The control group (n = 7) were treated with polycaprolactone/collagen/alginate (PCA) scaffold. Healing time, acoustic-mechanical properties, and morphological analysis were ...

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      Mentions: Byeong Ha Lee
    7. Examination of Gland Dropout Detected on Infrared Meibography by Using Optical Coherence Tomography Meibography

      Examination of Gland Dropout Detected on Infrared Meibography by Using Optical Coherence Tomography Meibography

      Purpose To elucidate the anatomic details of gland dropout detected on two-dimensional infrared (IR) meibography in cases of dry eye associated with meibomian gland dysfunction (MGD) by using three-dimensional optical coherence tomography (OCT) meibography. Methods In this cross-sectional, observational case series, we enrolled gland dropout detected on IR meibography; the condition was then examined using a real-time swept-source OCT system. Accordingly, a series of 500 raster B-scan OCT images, with the gland dropout site (observed on IR imaging) at the center, were obtained and rendered as three-dimensional volume images. The OCT images were classified based on the anatomic details, including ...

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    8. An All-Fiber-Optic Combined System of Noncontact Photoacoustic Tomography and Optical Coherence Tomography

      An All-Fiber-Optic Combined System of Noncontact Photoacoustic Tomography and Optical Coherence Tomography

      We propose an all-fiber-based dual-modal imaging system that combines noncontact photoacoustic tomography (PAT) and optical coherence tomography (OCT). The PAT remotely measures photoacoustic (PA) signals with a 1550-nm laser on the surface of a sample by utilizing a fiber interferometer as an ultrasound detector. The fiber-based OCT, employing a swept-source laser centered at 1310 nm, shares the sample arm of the PAT system. The fiber-optic probe for the combined system was homemade with a lensed single-mode fiber (SMF) and a large-core multimode fiber (MMF). The compact and robust common probe is capable of obtaining both the PA and the OCT ...

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    9. All optical fiber combined-imaging system of photoacoustic and optical coherence tomography

      All optical fiber combined-imaging system of photoacoustic and optical coherence tomography

      We present an all optical fiber combined-imaging system that integrates non-contact photoacoustic tomography (NPAT) and optical coherence tomography (OCT) to simultaneously provide PA and OCT images. The fiber-based PAT system utilizing a Mach-Zehnder interferometer with a fiber laser of 1550 nm measures the photoacoustic signal at the sample surface. For the generation of a PA signal, a pulse train from a bulk type Nd:YAG laser illuminates the sample via a large core multimode optical fiber. The fiber-based OCT operating at a center wavelength of 1310 nm allowed is combined with the fiber-based PAT system by sharing the same optical ...

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    10. Short cavity active mode locking fiber laser for optical sensing and imaging

      Short cavity active mode locking fiber laser for optical sensing and imaging

      We demonstrate a highly linear wavenumber- swept active mode locking (AML) fiber laser for optical sensing and imaging without any wavenumber-space resampling process. In this all-electric AML wavenumber-swept mechanism, a conventional wavelength selection filter is eliminated and, instead, the suitable programmed electric modulation signal is directly applied to the gain medium. Various types of wavenumber (or wavelength) tunings can be implemented because of the filter-less cavity configuration. Therefore, we successfully demonstrate a linearly wavenumber-swept AML fiber laser with 26.5 mW of output power to obtain an in-vivo OCT image at the 100 kHz swept rate.

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    11. Real-time stroboscopic full-field optical coherence tomography based on graphics processing unit

      Real-time stroboscopic full-field optical coherence tomography based on graphics processing unit

      We present the real-time stroboscopic full-field optical coherence tomography (FF-OCT) system that is based on graphics processing unit (GPU). The basic configuration of the proposed FF-OCT system was the Linnik interferometer. While scanning of a reference mirror in the axial direction, a series of the transverse sectional image was captured with a 2-dimensional CCD camera. To get a depth-resolved 3-D image, the light source of OCT was turned on and off like a stroboscope at the Doppler frequency of the OCT system. The CCD camera used in experiment operated at a rate of 200 frames per second, but the Doppler ...

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    12. Three-dimensional volumetric human meibomian gland investigation using polarization-sensitive optical coherence tomography

      Three-dimensional volumetric human meibomian gland investigation using polarization-sensitive optical coherence tomography

      In this study, polarization-sensitive optical coherence tomography (PS-OCT) capable of providing polarization contrasts such as phase retardation and degree of polarization uniformity (DOPU) was used for visualizing human meibomian glands (MGs) and investigating morphological characteristics of them. Especially, with the help of the DOPU contrast, MGs were exclusively extracted from the volumetric OCT image. In vivo PS-OCT measurements were performed on the upper eyelids of different age groups. From these measurements, different age-dependent aspects of the MG structure were also observed. Based on these observations, it can be inferred that the PS-OCT system has the potential for clinical diagnosis and ...

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    13. Segmentation method for in vivo meibomian gland OCT image

      Segmentation method for in vivo meibomian gland OCT image

      We demonstrate segmentation of human MGs based on several image processing technic. 3D volumetric data of upper eyelid was acquired from real-time FD-OCT, and its acini area of MGs was segmented. Three dimensional volume informations of meibomian glands should be helpful to diagnose meibomian gland related disease. In order to reveal boundary between tarsal plate and acini, each B-scan images were obtained before averaged three times. Imaging area was 10x10mm and 700x1000x500 voxels. The acquisition time was 60ms for B-scan and 30sec for C-scan. The 3D data was flattened to remove curvature and axial vibration, and resized to reduce computational ...

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    14. Refractive index measurements of multiple layers using numerical refocusing in FF-OCT

      Refractive index measurements of multiple layers using numerical refocusing in FF-OCT

      We propose and demonstrate the novel method of refractive index (RI) measurement for each layer of multilayered samples, which is based on numerical refocusing in full field optical coherence tomography (FF-OCT). The en-face FF-OCT image on an inner layer boundary of a multilayered sample is unintentionally blurred or defocused due to the RI of the sample itself, but can be numerically refocused. The refocusing is performed by numerically shifting the image sensor plane of the system, in general. However, by calculating the corresponding sample shift and then compared it with the actual sample shifting distance, we could extract the average ...

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    15. Single-camera polarization-sensitive full-field optical coherence tomography with polarization switch

      Single-camera polarization-sensitive full-field optical coherence tomography with polarization switch

      We present a single-channel detection-based polarization-sensitive full-field optical coherence tomography (PS-FF-OCT) for simultaneous acquisition of high-resolution OCT and linear retardance images. A linearly polarized sub-10-fs laser was used as a broadband light source for the OCT system. A bi-stable polarization switching device, composed of a ferroelectric liquid crystal cell and an analyzer, was employed to get the horizontal and the vertical polarization components of a full-field interference signal. The time-switched two perpendicular interference signals were sequentially recorded by a single charge-coupled device camera, then processed to extract en-face functional images of a biological sample. The rat tail tendon was imaged ...

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    16. Fiber-based optical coherence tomography for biomedical imaging, sensing, and precision measurements

      Fiber-based optical coherence tomography for biomedical imaging, sensing, and precision measurements

      Recently published studies on fiber-based optical coherence tomography (OCT) are reviewed mainly in relation to applications within the fields of biomedical imaging and precision measurement. A succinct introduction to fiber-based OCT system configurations is described including history, related core components, and functional characteristics. Then, an overview of fiber-optic probes is presented in terms of actuating method, scanning direction, and functionality. In order to verify the performance of fiber-optic OCT systems, fiber-based OCT images of several biological samples including pearls, fingers, teeth, and tumor tissues are presented. A multi-functional modality combined with laser induced fluorescence spectroscopy is also presented to distinguish ...

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    17. Single-step method for fiber-optic probe-based full-range spectral domain optical coherence tomography

      Single-step method for fiber-optic probe-based full-range spectral domain optical coherence tomography

      We propose a single-step method appropriated for a fiber-optic probe-based full-range spectral domain optical coherence tomography (OCT). The fiber-optic probe was scanned over a sample with a magnetically driven actuator. In the reference arm, a phase shift of π / 2 was applied during two neighbor axial scanning, from which the complex spectral interferogram was directly reconstructed. Since the complex-conjugate-free OCT image is obtained by doing just one Fourier transform on the complex interferogram, obtaining the full-range image is simple in algorithm and effective in computation time. Some full-range images of biological samples created with the proposed method are presented and the ...

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    18. In Vivo 3D Meibography of the Human Eyelid Using Real Time Imaging Fourier-Domain OCT

      In Vivo 3D Meibography of the Human Eyelid Using Real Time Imaging Fourier-Domain OCT

      Recently, we reported obtaining tomograms of meibomian glands from healthy volunteers using commercial anterior segment optical coherence tomography (AS-OCT), which is widely employed in clinics for examination of the anterior segment. However, we could not create 3D images of the meibomian glands, because the commercial OCT does not have a 3D reconstruction function. In this study we report the creation of 3D images of the meibomian glands by reconstructing the tomograms of these glands using high speed Fourier-Domain OCT (FD-OCT) developed in our laboratory. This research was jointly undertaken at the Department of Ophthalmology, Seoul St. Mary's Hospital (Seoul ...

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    19. Noninvasive Investigation of Deep Vascular Pathologies of Exudative Macular Diseases by High Penetration Optical Coherence Angiography

      Noninvasive Investigation of Deep Vascular Pathologies of Exudative Macular Diseases by High Penetration Optical Coherence Angiography

      Purpose: A newly developed high-penetration Doppler optical coherence angiography (HP-OCA) with a 1-μm probe beam for non-invasive investigation of vascular pathology of exudative macular diseases is introduced. A descriptive case series is presented to discuss the clinical utility of HP-OCA. Methods: Eleven eyes of 10 subjects with exudative macular disease, including 2 eyes with myopic choroidal neovascularization (mCNV), 4 eyes with age-related macular degeneration (AMD) and 5 eyes with polypoidal choroidal vasculopathy (PCV), were investigated. Two Doppler scanning modes (bi-directional and high-sensitive) of HP-OCA were used for the investigation. HP-OCA provides depth resolved and en face angiograms and a ...

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    20. Polarization sensitive full-field optical coherence tomography based on bi-stable polarizaton switch

      Polarization sensitive full-field optical coherence tomography based on bi-stable polarizaton switch

      We present polarization-sensitive full-field optical coherence tomography (PS-FF-OCT), which is based on a bi-stable polarization switch (BSPS) device. The proposed PS-FF-OCT is a Linnik type interferometer, and allows getting both the birefringence-induced phase retardation and the intensity images of specimens with high resolutions using a pair of micro objectives and a BSPS device. Two orthogonal polarization states are formed with a regular time interval by the BSPS device that changes the polarization direction of light in a short time by switching its optic axes. Therefore, both the horizontally polarized light signal and the vertically polarized light signal from the sample ...

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    21. Feature Of The Week 8/26/12: Gwangju Institute of Science and Technology Investigate Tomography Imaging of Single Living Cell Using Optical Tweezers and FF-OCT

      Feature Of The Week 8/26/12: Gwangju Institute of Science and Technology Investigate Tomography Imaging of Single Living Cell Using Optical Tweezers and FF-OCT

      Gwangju Institute of Science and recently proposed a label-free depth-resolved tomographic scheme for imaging a single live cell in fluid. This approach utilizes a modified time-domain full-field optical coherence tomography (FF-OCT) system combined with an optical tweezer technique. The optical trap for holding a moving specimen is made by tightly focusing a 1064 nm Q -switching pulsed laser beam with a 1.0 NA microscope objective in the sample arm of the FF-OCT part. By cosharing the probe for both systems, the optical actions of trapping and cellular resolution tomographic imaging could be achieved simultaneously. Feasibility of the combined system ...

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    22. Full range spectral domain optical coherence tomography using a fiber-optic probe as a self-phase shifter

      Full range spectral domain optical coherence tomography using a fiber-optic probe as a self-phase shifter

      We present a full range handheld probe type spectral domain optical coherence tomography (SD-OCT) method. Here, the sample arm is composed of a tilted fiber-optic cantilever scanner; thus, the phase shift concurrently occurs while sample scanning. With the phase shift, we could achieve a full range complex-conjugate-free OCT image with no additional phase shifters in the reference arm. To realize this technique, a magnetically actuated probe was adopted. Full range SD-OCT images of a pearl, human fingernail, and human tooth were subsequently obtained using this suggested probe. The scanning range and acquisition speed were 3 mm and 20  frames/s ...

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    23. Tomographic imaging of a suspending single live cell using optical tweezer-combined full-field optical coherence tomography

      Tomographic imaging of a suspending single live cell using optical tweezer-combined full-field optical coherence tomography

      We propose a label-free depth-resolved tomographic scheme for imaging a single live cell in fluid. This approach utilizes a modified time-domain full-field optical coherence tomography (FF-OCT) system combined with an optical tweezer technique. The optical trap for holding a moving specimen is made by tightly focusing a 1064 nm Q -switching pulsed laser beam with a 1.0 NA microscope objective in the sample arm of the FF-OCT part. By cosharing the probe for both systems, the optical actions of trapping and cellular resolution tomographic imaging could be achieved simultaneously. Feasibility of the combined system is demonstrated by imaging micron-sized ...

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    24. Improvement of axial resolution of spectral domain optical coherence tomography with wide band PLC splitter

      Improvement of axial resolution of spectral domain optical coherence tomography with wide band PLC splitter

      We report the development and performance of high resolution spectral domain optical coherence tomography (SD-OCT) system based on 2×2 planar lightwave circuit (PLC) splitter that was designed as a single mode splitter at near infrared and used as the beam splitter for a SD-OCT system. The splitter has been made by coupling SMFs to a planar lightwave circuit (PLC) splitter chip. The PLC splitter chip was fabricated to have a single mode property with 740 nm cutoff wavelength and the SMFs, which have 730 nm cutoff wavelength, were securely connected to the PLC chip through fiber block arrays having ...

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    1-24 of 55 1 2 3 »
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    1. (54 articles) Gwangju Institute of Science and Technology
    2. (54 articles) Byeong Ha Lee
    3. (16 articles) Woo June Choi
    4. (15 articles) Jun Geun Shin
    5. (14 articles) Jihoon Na
    6. (13 articles) Seon Young Ryu
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    Optical approach for monitoring the periodontal ligament changes induced by orthodontic forces around maxillary anterior teeth of white rats Ultrawideband photonic crystal fiber coupler for multiband optical imaging system Evaluating and identifying pearls and their nuclei by using optical coherence tomography Full-field optical coherence microscopy for identifying live cancer cells by quantitative measurement of refractive index distribution Feasibility of Full-field Optical Coherence Microscopy in Ultra-structural Imaging of Human Colon Tissues Feature Of The Week 3/27/11: Feasibility of Full-Field Optical Coherence Tomography for Detection of Counterfeit Paper Money Characterization of wet pad surface in chemical mechanical polishing (CMP) process with full-field optical coherence tomography (FF-OCT) Vitreous Seeding in Retinocytoma: Importance of Optical Coherence Tomography Utility of Spectral-Domain Optical Coherence Tomography in Differentiating Papilledema From Pseudopapilledema: A Prospective Longitudinal Study Visualizing and quantifying cutaneous microvascular reactivity in humans using optical coherence tomography: Impaired dilator function in diabetes Optical Coherence Tomography of Outer Retinal Hyper-Reflectivity, Neovascularization and Pigment in Type 2 Macular Telangiectasia Early Detection of Incipient Retinal Pigment Epithelium Atrophy Overlying Drusen with Fundus Autofluorescence vs. Spectral Domain Optical Coherence Tomography