All Articles from Daniel X. Hammer -Optical Coherence Tomography News

Articles from Daniel X. Hammer

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1. Direct laser writing of a titanium dioxide-laden retinal cone phantom for adaptive optics-optical coherence tomography
  
  Explore OSA | OSA Publishing Oct 10 2020 Ophthalmology
  
  The spatial resolution of adaptive optics-optical coherence tomography (AO-OCT) enables visualization of retinal components ( e.g ., photoreceptors), which can advance clinical diagnosis of ocular diseases. Currently, however, variability in AO-OCT system performance suggests a need for standardized physical models, or “phantoms”, that replicate the opto-structural properties of retinal components. Here we employ direct laser writing – a two-photon polymerization-based additive manufacturing technology – using a light scattering titanium (IV) dioxide nanoparticle-laden photomaterial to create novel retinal cone outer segment (OS) phantoms. Fabrication results demonstrate effective mimicry of cone OS diameter and spacing at four retinal eccentricities, while AO-OCT experiments demonstrate cone spacings ...
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  Mentions: University of Maryland FDA
2. Application of optical coherence tomography and optical path length method for monitoring corneal thickness and refractive index change during corneal cross-linking
  
  Explore OSA | OSA Publishing Jun 3 2019 Ophthalmology
  
  $Application of optical coherence tomography and optical path length method for monitoring corneal thickness and refractive index change during corneal cross-linking$
  Corneal cross-linking (CXL) using UVA irradiation with a riboflavin photosensitizer has emerged as a new treatment paradigm for corneal ectatic disorders. The thickness threshold for protection of intraocular structures has often been challenged with ongoing developments, and corneal thinning becomes an important safety concern, especially for patients with thin corneas. In this study with an ex vivo bovine eye model, we monitored corneal thinning and corneal refractive index changes using optical coherence tomography (OCT) integrated with an adaptation of the optical path length method. CXL experiments were performed based on the standard protocol that includes removal of the corneal epithelium ...
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  Mentions: Daniel X. Hammer FDA Anant Agrawal
3. Toward optical coherence tomography angiography-based biomarkers to assess the safety of peripheral nerve electrostimulation
  
  Explore IOPscience Apr 2 2019 Neurology
  
  Objective: Peripheral nerves serve as a link between the central nervous system and its targets. Altering peripheral nerve activity through targeted electrical stimulation is being investigated as a therapy for modulating end organ function. To support rapid advancement in the field, novel approaches to predict and prevent nerve injury resulting from the electrical stimulation must be developed to overcome the limitations of traditional histological methods. The present study aims to develop an optical imaging-based approach for real-time assessment of peripheral nerve injury associated with electrical stimulation.  Approach: We developed an optical coherence tomography angiography system and a 3-D ...
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  Mentions: Massachusetts General Hospital Daniel X. Hammer Harvard University
4. 3-D printed photoreceptor phantoms for evaluating lateral resolution of adaptive optics imaging systems
  
  Explore OSA | OSA Publishing Apr 2 2019
  
  With adaptive optics (AO), optical coherence tomography and scanning laser ophthalmoscopy imaging systems can resolve individual photoreceptor cells in living eyes, due to enhanced lateral spatial resolution. However, no standard test method exists for experimentally quantifying this parameter in ophthalmic AO imagers. Here, we present three-dimensional (3-D) printed phantoms, which enable the measurement of lateral resolution in an anatomically relevant manner. We used two-photon polymerization to fabricate two phantoms, which mimic the mosaic of cone photoreceptor outer segments at multiple retinal eccentricities. With these phantoms, we demonstrated that the resolution of two multimodal AO systems is similar to theoretical predictions ...
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  Mentions: University of Maryland National Institutes of Health Daniel X. Hammer
5. Optical coherence tomography angiography based biomarkers to assess the safety of peripheral nerve electrostimulation
  
  Explore SPIE Digital Library Mar 20 2018
  
  Peripheral nerves connect and relay information between the central nervous system and its target organs. Small arteries traverse the epineurium and are responsible for supplying blood to the axons and cells within the nerves. Constriction or damage to these vessels can reduce perfusion leading to ischemic insults. Peripheral nerve electrostimulation has been approved for the treatment of epilepsy, depression and migraines, and is also being studied for the treatment of rheumatoid arthritis, Crohn’s disease, polycystic ovary syndrome, and type II diabetes. While the safety and efficacy of currently approved medical devices is well established, next generation devices may require ...
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  Mentions: Massachusetts General Hospital Daniel X. Hammer Harvard University
6. Optic Nerve Head Measurements With Optical Coherence Tomography: A Phantom-Based Study Reveals Differences Among Clinical Devices
  
  Explore IOVS Journal Jul 14 2016 Ophthalmology
  
  Purpose : Optical coherence tomography (OCT) can monitor for glaucoma by measuring dimensions of the optic nerve head (ONH) cup and disc. Multiple clinical studies have shown that different OCT devices yield different estimates of retinal dimensions. We developed phantoms mimicking ONH morphology as a new way to compare ONH measurements from different clinical OCT devices. Methods : Three phantoms were fabricated to model the ONH: One normal and two with glaucomatous anatomies. Phantoms were scanned with Stratus, RTVue, and Cirrus clinical devices, and with a laboratory OCT system as a reference. We analyzed device-reported ONH measurements of cup-to-disc ratio (CDR) and ...
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  Mentions: University of Pittsburgh Joel S. Schuman Daniel X. Hammer
7. Feature of the Week 03/20/2016: Closed Loop Tracked Doppler OCT Based Heart Monitor for the Drosophila Melanogaster Larvae
  
  Explore Optical Coherence Tomography News Mar 20 2016 Developmental Biology
  
  A fruit fly larva (top) was continuously tracked for continuous remote monitoring. A heartbeat trace of freely moving larva (bottom) was obtained by a low coherence interferometry based doppler sensing technique. This paper presents a novel instrument for biosciences, useful for studies of moving embryos. A dual sequential imaging/measurement channel is assembled via a closed-loop tracking architecture. The dual channel system can operate in two regimes: (i) single-point Doppler signal monitoring or (ii) fast 3-D swept source OCT imaging. The system is demonstrated for characterizing cardiac dynamics in Drosophila melanogaster larva. Closed loop tracking enables long term in vivo ...
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  Mentions: Adrian G. Podoleanu University of Kent Daniel X. Hammer
8. IMAGING WITH MULTIMODAL ADAPTIVE-OPTICS OPTICAL COHERENCE TOMOGRAPHY IN MULTIPLE EVANESCENT WHITE DOT SYNDROME: THE STRUCTURE AND FUNCTIONAL RELATIONSHIP
  
  Explore LWW Online Jan 7 2016 Ophthalmology
  
  Purpose: To elucidate the location of pathological changes in multiple evanescent white dot syndrome (MEWDS) with the use of multimodal adaptive optics (AO) imaging . Methods: A 5-year observational case study of a 24-year-old female with recurrent MEWDS. Full examination included history, Snellen chart visual acuity, pupil assessment, intraocular pressures, slit lamp evaluation, dilated fundoscopic exam, imaging with Fourier-domain optical coherence tomography (FD-OCT), blue-light fundus autofluorescence (FAF), fundus photography, fluorescein angiography, and adaptive-optics optical coherence tomography . Results: Three distinct acute episodes of MEWDS occurred during the period of follow-up. Fourier-domain optical coherence tomography and adaptive-optics imaging showed disturbance in the photoreceptor ...
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  Mentions: University of Pittsburgh Joel S. Schuman Daniel X. Hammer
9. Image quality metrics for optical coherence angiography
  
  Explore OSA | OSA Publishing Jun 20 2015 Ophthalmology
  
  We characterized image quality in optical coherence angiography (OCA) en face planes of mouse cortical capillary network in terms of signal-to-noise ratio (SNR) and Weber contrast (Wc) through a novel mask-based segmentation method. The method was used to compare two adjacent B-scan processing algorithms, (1) average absolute difference (AAD) and (2) standard deviation (SD), while varying the number of lateral cross-sections acquired (also known as the gate length, N). AAD and SD are identical at N = 2 and exhibited similar image quality for N<10. However, AAD is relatively less susceptible to bulk tissue motion artifact than SD. SNR ...
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  Mentions: Daniel X. Hammer FDA Anant Agrawal
10. Closed loop tracked Doppler optical coherence tomography based heart monitor for the Drosophila melanogaster larvae
  
  Explore Wiley Online Library Apr 30 2015 Developmental Biology
  
  A fruit fly larva (top) was continuously tracked for continuous remote monitoring. A heartbeat trace of freely moving larva (bottom) was obtained by a low coherence interferometry based doppler sensing technique. This paper presents a novel instrument for biosciences, useful for studies of moving embryos. A dual sequential imaging/measurement channel is assembled via a closed-loop tracking architecture. The dual channel system can operate in two regimes: (i) single-point Doppler signal monitoring or (ii) fast 3-D swept source OCT imaging. The system is demonstrated for characterizing cardiac dynamics in Drosophila melanogaster larva. Closed loop tracking enables long term in vivo ...
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  Mentions: Adrian G. Podoleanu University of Kent Daniel X. Hammer
11. The use of time-lapse optical coherence tomography to image the effects of microapplied toxins on the retina
  
  Explore iovs.org Dec 20 2014 Ophthalmology
  
  Purpose: We developed a novel technique using a drug microperfusion device and time-lapse optical coherence tomography (OCT) for accelerated drug screening and retinotoxin characterization. Methods: Using an ex vivo rabbit eyecup preparation, we studied retinotoxin effects in real-time by microperfusing small retinal areas under a transparent fluoropolymer tube. Known retinotoxic agents were applied to the retina for 5 minute periods while changes in retinal structure, thickness, and reflectance were monitored with OCT. The OCT images of two agents with dissimilar mechanisms, cyanide and kainic acid, were compared with their structural changes seen histologically. Results: We found the actions of retinotoxic ...
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  Mentions: Daniel X. Hammer FDA Rice University
12. Feature Of The Week 11/2/14: Longitudinal Vascular Dynamics Following Cranial Window and Electrode Implantation Measured with Speckle Variance Optical Coherence Angiography
  
  Explore Optical Coherence Tomography News Nov 2 2014
  
  FDA researchers Daniel X. Hammer, Andrea Lozzi, Erkinay Abliz, Noah Greenbaum, Anant Agrawal, Victor Krauthamer, and Cristin G. Welle published a paper in Biomedical Optics Express titled, “Longitudinal vascular dynamics following cranial window and electrode implantation measured with speckle variance optical coherence angiography.” The paper represents the first attempt to characterize vascular remapping associated with chronic neural implants using speckle variance optical coherence angiography (SV-OCA). SV-OCA is an innovative vascular imaging technique that relies on speckle pattern changes from moving blood, rather than the injection of extrinsic dyes or contrast agents, allowing visualization of the complete volumetric capillary network in ...
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  Mentions: Daniel X. Hammer FDA Anant Agrawal
13. In Vivo Three-Dimensional Characterization of the Healthy Human Lamina Cribrosa with Adaptive Optics Spectral-Domain Optical Coherence Tomography
  
  Explore iovs.org Sep 19 2014 Ophthalmology
  
  Purpose: To characterize the in vivo 3D lamina cribrosa (LC) microarchitecture of healthy eyes using adaptive optics spectral-domain optical coherence tomography (AO-SDOCT). Methods: One randomly selected eye from each of 18 healthy subjects was scanned with Cirrus HD-OCT and AO-SDOCT centered on the optic nerve head. LC microarchitecture, imaged by the later device, was semi-automatically segmented and quantified for connective tissue volume fraction (CTVF), beam thickness, pore diameter, pore area and pore aspect ratio. The LC was assessed in central and peripheral regions of equal areas, quadrants and with depth. A linear mixed-effects model weighted by the fraction of visible ...
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  Mentions: University of Pittsburgh Joel S. Schuman Larry Kagemann
14. Longitudinal vascular dynamics following cranial window and electrode implantation measured with speckle variance optical coherence angiography
  
  Explore opticsinfobase.org Aug 2 2014
  
  Speckle variance optical coherence angiography (OCA) was used to characterize the vascular tissue response from craniotomy, window implantation, and electrode insertion in mouse motor cortex. We observed initial vasodilation ~40% greater than original diameter 2-3 days post-surgery (dps). After 4 weeks, dilation subsided in large vessels (>50 µm diameter) but persisted in smaller vessels (25-50 µm diameter). Neovascularization began 8-12 dps and vessel migration continued throughout the study. Vasodilation and neovascularization were primarily associated with craniotomy and window implantation rather than electrode insertion. Initial evidence of capillary re-mapping in the region surrounding the implanted electrode was manifest in OCA image ...
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  Mentions: Daniel X. Hammer FDA Anant Agrawal
15. Adaptive optics optical coherence tomography with dynamic retinal tracking
  
  Explore opticsinfobase.org Jun 17 2014 Ophthalmology
  
  Adaptive optics optical coherence tomography (AO-OCT) is a highly sensitive and noninvasive method for three dimensional imaging of the microscopic retina. Like all in vivo retinal imaging techniques, however, it suffers the effects of involuntary eye movements that occur even under normal fixation. In this study we investigated dynamic retinal tracking to measure and correct eye motion at KHz rates for AO-OCT imaging. A customized retina tracking module was integrated into the sample arm of the 2nd-generation Indiana AO-OCT system and images were acquired on three subjects. Analyses were developed based on temporal amplitude and spatial power spectra in conjunction ...
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  Mentions: Daniel X. Hammer Qiang Wang Ravi S. Jonnal
16. Repeatability of in vivo 3D lamina cribrosa microarchitecture using adaptive optics spectral domain optical coherence tomography
  
  Explore opticsinfobase.org Mar 10 2014 Ophthalmology
  
  We demonstrate the repeatability of lamina cribrosa (LC) microarchitecture for in vivo 3D optical coherence tomography (OCT) scans of healthy, glaucoma suspects, and glaucomatous eyes. Eyes underwent two scans using a prototype adaptive optics spectral domain OCT (AO-SDOCT) device from which LC microarchitecture was semi-automatically segmented. LC segmentations were used to quantify pore and beam structure through several global microarchitecture parameters. Repeatability of LC microarchitecture was assessed qualitatively and quantitatively by calculating parameter imprecision. For all but one parameters (pore volume) measurement imprecision was <4.7% of the mean value, indicating good measurement reproducibility. Imprecision ranged between 27.3% and ...
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  Mentions: University of Pittsburgh Joel S. Schuman Larry Kagemann
17. Optical coherence tomography-based micro-particle image velocimetry
  
  Explore opticsinfobase.org Nov 1 2013
  
  We present a new application of optical coherence tomography (OCT), widely used in biomedical imaging, to flow analysis in near-wall hydrodynamics for marine research. This unique capability, called OCT micro-particle image velocimetry, provides a high-resolution view of microscopic flow phenomena and measurement of flow statistics within the first millimeter of a boundary layer. The technique is demonstrated in a small flow cuvette and in a water tunnel.
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  Mentions: Daniel X. Hammer Physical Sciences Incorporated R. Daniel Ferguson
18. Automated lamina cribrosa microstructural segmentation in optical coherence tomography scans of healthy and glaucomatous eyes
  
  Explore opticsinfobase.org Oct 24 2013 Ophthalmology
  
  We demonstrate an automated segmentation method for in-vivo 3D optical coherence tomography (OCT) imaging of the lamina cribrosa (LC). Manual segmentations of coronal slices of the LC were used as a gold standard in parameter selection and evaluation of the automated technique. The method was validated using two prototype OCT devices; each had a subject cohort including both healthy and glaucomatous eyes. Automated segmentation of in-vivo 3D LC OCT microstructure performed comparably to manual segmentation and is useful for investigative research and in clinical quantification of the LC.
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  Mentions: University of Pittsburgh Massachusetts Institute of Technology James G. Fujimoto
19. Retina-simulating phantom for optical coherence tomography
  
  Explore SPIE | Journal Sep 18 2013 Ophthalmology
  
  Optical coherence tomography (OCT) is a rapidly growing imaging modality, particularly in the field of ophthalmology. Accurate early diagnosis of diseases requires consistent and validated imaging performance. In contrast to more well-established medical imaging modalities, no standardized test methods currently exist for OCT quality assurance. We developed a retinal phantom which mimics the thickness and near-infrared optical properties of each anatomical retinal layer as well as the surface topography of the foveal pit. The fabrication process involves layer-by-layer spin coating of nanoparticle-embedded silicone films followed by laser micro-etching to modify the surface topography. The thickness of each layer and dimensions ...
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  Mentions: Johns Hopkins University Daniel X. Hammer FDA
20. Accurate prediction of collapse temperature using optical coherence tomography-based freeze-drying microscopy
  
  Explore Wiley Online Library May 19 2013 NDE/NDT , Other Non-Medical
  
  The objective of this study was to assess the feasibility of developing and applying a laboratory tool that can provide three-dimensional product structural information during freeze-drying and which can accurately characterize the collapse temperature ( T c ) of pharmaceutical formulations designed for freeze-drying. A single-vial freeze dryer coupled with optical coherence tomography freeze-drying microscopy (OCT–FDM) was developed to investigate the structure and T c of formulations in pharmaceutically relevant products containers (i.e., freeze-drying in vials). OCT–FDM was used to measure the T c and eutectic melt of three formulations in freeze-drying vials. The T c as measured by ...
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  Mentions: Daniel X. Hammer Physical Sciences Incorporated University of Connecticut
21. Multimodal adaptive optics retinal imager: design and performance
  
  Explore opticsinfobase.org Nov 20 2012 Ophthalmology
  
  Optical coherence tomography (OCT) and scanning laser ophthalmoscopy (SLO) are complementary imaging modalities, the combination of which can provide clinicians with a wealth of information to detect retinal diseases, monitor disease progression, or assess new therapies. Adaptive optics (AO) is a tool that enables correction of wavefront distortions from ocular aberrations. We have developed a multimodal adaptive optics system (MAOS) for high-resolution multifunctional use in a variety of research and clinical applications. The system integrates both OCT and SLO imaging channels into an AO beam path. The optics and hardware were designed with specific features for simultaneous SLO/OCT output ...
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  Mentions: Daniel X. Hammer Harvard University Physical Sciences Incorporated
22. Adaptive optics line scanning ophthalmoscope
  
  Explore uspto.gov Jun 19 2012 Ophthalmology , Patents
  
  A first optical module scans a portion of an eye with a line of light, descans reflected light from the scanned portion of the eye and confocally provides output light in a line focus configuration. A detection device detects the output light and images the portion of the eye. A second optical module detects an optical distortion and corrects the optical distortion in the line of light scanned on the portion of the eye.
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  Mentions: Daniel X. Hammer Physical Sciences Incorporated R. Daniel Ferguson
23. Imaging flow dynamics in murine coronary arteries with spectral domain optical Doppler tomography
  
  Explore opticsinfobase.org Mar 13 2012 Cardiology
  
  Blood flow in murine epicardial and intra-myocardial coronary arteries was measured in vivo with spectral domain optical Doppler tomography (SD-ODT). Videos at frame rates up to 180 fps were collected and processed to extract phase shifts associated with moving erythrocytes in the coronary arteries. Radial averaging centered on the vessel lumen provided spatial smoothing of phase noise in a single cross-sectional frame for instantaneous peak velocity measurement without distortion of the flow profile. Temporal averaging synchronized to the cardiac cycle (i.e., gating) was also performed to reduce phase noise, although resulting in lower flow profiles. The vessel angle with ...
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  Mentions: Daniel X. Hammer Thomas E. Milner Marc D. Feldman
24. Fluorescence-guided optical coherence tomography imaging for colon cancer screening: a preliminary mouse study
  
  Explore opticsinfobase.org Dec 19 2011 Oncology
  
  A new concept for cancer screening has been preliminarily investigated. A cancer targeting agent loaded with a near-infrared (NIR) dye was topically applied on the tissue to highlight cancer-suspect locations and guide optical coherence tomography (OCT) imaging, which was used to further investigate tissue morphology at the micron scale. A pilot study on ApcMin mice has been performed to preliminarily test this new cancer screening approach. As a cancer-targeting agent, poly(epsilon-caprolactone) microparticles (PCLMPs), labeled with a NIR dye and functionalized with an RGD (argenine-glycine-aspartic acid) peptide, were used. This agent recognizes the ανβ3 integrin receptor (ABIR), which is over-expressed ...
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  Mentions: Daniel X. Hammer Physical Sciences Incorporated R. Daniel Ferguson
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