All Articles from Elliott D. SoRelle -Optical Coherence Tomography News

Articles from Elliott D. SoRelle

1-11 of 11
1. Developing in vivo Functional Imaging Technology with Micron-Scale Resolution Using Optical Coherence Tomography
  
  Explore MathWorks Aug 20 2018
  
  Researchers and physicians rely on functional imaging to better understand tumors and other structures within the human body. However, imaging technologies that capture deep structures have poor resolution, while those that provide high resolution have limited depth. Positron emission tomography (PET), for example, reveals details deep within tissue but suffers from poor spatial resolution, with each voxel of a PET scan representing thousands or even millions of cells. In contrast, optical microscopy can deliver subcellular spatial resolution but is usually limited to a depth of tens of microns. Optical coherence tomography (OCT) helps bridge the gap between low-resolution/high-penetration and ...
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  Mentions: Stanford University Adam de la Zerda Elliott D. SoRelle
2. Developing in vivo Functional Imaging Technology with Micron-Scale Resolution Using Optical Coherence Tomography
  
  Explore MathWorks Nov 3 2017
  
  Researchers and physicians rely on functional imaging to better understand tumors and other structures within the human body. However, imaging technologies that capture deep structures have poor resolution, while those that provide high resolution have limited depth. Positron emission tomography (PET), for example, reveals details deep within tissue but suffers from poor spatial resolution, with each voxel of a PET scan representing thousands or even millions of cells. In contrast, optical microscopy can deliver subcellular spatial resolution but is usually limited to a depth of tens of microns. Optical coherence tomography (OCT) helps bridge the gap between low-resolution/high-penetration and ...
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  Mentions: Stanford University Adam de la Zerda Elliott D. SoRelle
3. Speckle-modulating optical coherence tomography in living mice and humans
  
  Explore Nature Publishing Group Jun 20 2017
  
  Optical coherence tomography (OCT) is a powerful biomedical imaging technology that relies on the coherent detection of backscattered light to image tissue morphology in vivo . As a consequence, OCT is susceptible to coherent noise (speckle noise), which imposes significant limitations on its diagnostic capabilities. Here we show speckle-modulating OCT (SM-OCT), a method based purely on light manipulation that virtually eliminates speckle noise originating from a sample. SM-OCT accomplishes this by creating and averaging an unlimited number of scans with uncorrelated speckle patterns without compromising spatial resolution. Using SM-OCT, we reveal small structures in the tissues of living animals, such as ...
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  Mentions: Optovue Thorlabs Stanford University
4. Spectral contrast-enhanced optical coherence tomography for improved detection of tumor microvasculature and functional imaging of lymphatic drainage
  
  Explore Conference Proceedings Mar 9 2017
  
  Optical Coherence Tomography (OCT) is well-suited to study in vivo dynamics of blood circulation and lymphatic flow because of the technique’s combination of rapid image acquisition, micron spatial resolution, and penetration depth in turbid tissues. However, OCT has been historically constrained by a dearth of contrast agents that are readily distinguished from the strong scattering intrinsic to biological tissues. In this study, we demonstrate large gold nanorods (LGNRs) as optimized contrast agents for OCT. LGNRs produce 32-fold greater backscattering than GNRs previously tested for contrast-enhanced OCT. Furthermore, LGNRs exhibit 110-fold stronger spectral signal than conventional GNRs when coupled with ...
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  Mentions: Stanford University Adam de la Zerda Debasish Sen
5. Reconstruction and Spectral Analysis for Optical Coherence Tomography
  
  Explore MathWorks Mar 1 2017
  
  MATLAB code for reconstruction and spectral analysis of spectral domain OCT images. This code can be used as part of a platform for molecular imaging with OCT, which we call MOZART. This code was created to read raw interferograms from Thorlabs OCTs (SW version 4 works best, but version 3 is also supported with a few changes). It reconstructs the raw interferograms into OCT images, and supports both 2D, 3D and speckle variance. In addition to reconstructing the images this code: Calculates the normalized spcekle variance (useful for detecting blood vessels) Calculates dispersion compensation Calculates a map of spectral contras ...
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  Mentions: Thorlabs Stanford University Adam de la Zerda
6. High sensitivity contrast enhanced optical coherence tomography for functional in vivo imaging
  
  Explore Conference Proceedings Feb 23 2017
  
  In this study, we developed and applied highly-scattering large gold nanorods (LGNRs) and custom spectral detection algorithms for high sensitivity contrast-enhanced optical coherence tomography (OCT). We were able to detect LGNRs at a concentration as low as 50 pM in blood. We used this approach for noninvasive 3D imaging of blood vessels deep in solid tumors in living mice. Additionally, we demonstrated multiplexed imaging of spectrally-distinct LGNRs that enabled observations of functional drainage in lymphatic networks. This method, which we call MOZART, provides a platform for molecular imaging and characterization of tissue noninvasively at cellular resolution
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  Mentions: Stanford University Adam de la Zerda Debasish Sen
7. Developing in vivo Functional Imaging Technology with Micron-Scale Resolution Using Optical Coherence Tomography
  
  Explore MathWorks Feb 20 2017
  
  Researchers and physicians rely on functional imaging to better understand tumors and other structures within the human body. However, imaging technologies that capture deep structures have poor resolution, while those that provide high resolution have limited depth. Positron emission tomography (PET), for example, reveals details deep within tissue but suffers from poor spatial resolution, with each voxel of a PET scan representing thousands or even millions of cells. In contrast, optical microscopy can deliver subcellular spatial resolution but is usually limited to a depth of tens of microns. Optical coherence tomography (OCT) helps bridge the gap between low-resolution/high-penetration and ...
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  Mentions: Stanford University Adam de la Zerda Elliott D. SoRelle
8. High-resolution contrast-enhanced optical coherence tomography in mice retinae
  
  Explore SPIE | Journal Jun 11 2016 Developmental Biology , Ophthalmology
  
  Optical coherence tomography (OCT) is a noninvasive interferometric imaging modality providing anatomical information at depths of millimeters and a resolution of micrometers. Conventional OCT images limit our knowledge to anatomical structures alone, without any contrast enhancement. Therefore, here we have, for the first time, optimized an OCT-based contrast-enhanced imaging system for imaging single cells and blood vessels in vivo inside the living mouse retina at subnanomolar sensitivity. We used bioconjugated gold nanorods (GNRs) as exogenous OCT contrast agents. Specifically, we used anti-mouse CD45 coated GNRs to label mouse leukocytes and mPEG-coated GNRs to determine sensitivity of GNR detection in vivo ...
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  Mentions: Stanford University Adam de la Zerda Debasish Sen
9. Feature of the Week 04/02/2016: Contrast-Enhanced Optical Coherence Tomography with Picomolar Sensitivity for Functional in vivo Imaging (with Audio)
  
  Explore Optical Coherence Tomography News Apr 2 2016
  
  Optical Coherence Tomography (OCT) enables real-time imaging of living tissues with cellular resolution over large 3D fields of view.[1] However, functional and molecular capabilities for OCT remain elusive due to the difficulties of distinguishing exogenous contrast agents from intrinsic tissue scattering and absorption. Previous reports have detailed the use of magnetic probes,[2] absorbent dyes,[3] and gold nanoparticles[4] to produce OCT contrast enhancement through various mechanisms. In the current study, optimized large gold nanorods (LGNRs) and a customized aberration-free spectral detection algorithm were developed to demonstrate an improved platform for molecular imaging with OCT. LGNRs, which produce ...
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  Mentions: Stanford University Adam de la Zerda Debasish Sen
10. Contrast-enhanced optical coherence tomography with picomolar sensitivity for functional in vivo imaging
  
  Explore Nature Publishing Group Mar 19 2016
  
  Optical Coherence Tomography (OCT) enables real-time imaging of living tissues at cell-scale resolution over millimeters in three dimensions. Despite these advantages, functional biological studies with OCT have been limited by a lack of exogenous contrast agents that can be distinguished from tissue. Here we report an approach to functional OCT imaging that implements custom algorithms to spectrally identify unique contrast agents: large gold nanorods (LGNRs). LGNRs exhibit 110-fold greater spectral signal per particle than conventional GNRs, which enables detection of individual LGNRs in water and concentrations as low as 250 pM in the circulation of living mice. This translates to ...
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  Mentions: Stanford University Adam de la Zerda Debasish Sen
11. Quantitative contrast-enhanced optical coherence tomography
  
  Explore Scitation Jan 1 2016
  
  We have developed a model to accurately quantify the signals produced by exogenous scattering agents used for contrast-enhanced Optical Coherence Tomography (OCT). This model predicts distinct concentration-dependent signal trends that arise from the underlying physics of OCT detection. Accordingly, we show that real scattering particles can be described as simplified ideal scatterers with modified scattering intensity and concentration. The relation between OCT signal and particle concentration is approximately linear at concentrations lower than 0.8 particle per imaging voxel. However, at higher concentrations, interference effects cause signal to increase with a square root dependence on the number of particles within ...
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  Mentions: Stanford University Adam de la Zerda Elliott D. SoRelle
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