All Articles from Olivier Thouvenin -Optical Coherence Tomography News

Articles from Olivier Thouvenin

1-8 of 8
1. Full-Field Optical Coherence Tomography as a Diagnosis Tool: Recent Progress with Multimodal Imaging
  
  Explore MDPI Open Access Journals Platform Mar 2 2017
  
  Full-field optical coherence tomography (FF-OCT) is a variant of OCT that is able to register 2D en face views of scattering samples at a given depth. Thanks to its superior resolution, it can quickly reveal information similar to histology without the need to physically section the sample. Sensitivity and specificity levels of diagnosis performed with FF-OCT are 80% to 95% of the equivalent histological diagnosis performances and could therefore benefit from improvement. Therefore, multimodal systems have been designed to increase the diagnostic performance of FF-OCT. In this paper, we will discuss which contrasts can be measured with such multimodal systems ...
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  Mentions: Claude Boccara LLTech ESPCI ParisTech
2. Dynamic multimodal full-field optical coherence tomography and fluorescence structured illumination microscopy
  
  Explore SPIE | Journal Feb 15 2017 Developmental Biology
  
  We report on the development of a configuration of a multimodal full-field optical coherence tomography (FF-OCT) and fluorescence microscope. Our system can simultaneously acquire FF-OCT and structured illumination microscopy images. Dynamic parallel evolution of tissue microstructures and biochemical environments can be visualized. We use high numerical aperture objectives to optimize the combination of the two modalities. We imaged the propagation of mechanical waves initiated by calcium waves in a heart wall to illustrate the interest of simultaneous recording of mechanical and biochemical information.
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  Mentions: Claude Boccara ESPCI ParisTech Mathias Fink
3. En face coherence microscopy [Invited]
  
  Explore OSA | OSA Publishing Jan 7 2017 Microscopy
  
  En face coherence microscopy or flying spot or full field optical coherence tomography or microscopy (FF-OCT/FF-OCM) belongs to the OCT family because the sectioning ability is mostly linked to the source coherence length. In this article we will focus our attention on the advantages and the drawbacks of the following approaches: en face versus B scan tomography in terms of resolution, coherent versus incoherent illumination and influence of aberrations, and scanning versus full field imaging. We then show some examples to illustrate the diverse applications of en face coherent microscopy and show that endogenous or exogenous contrasts can add ...
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  Mentions: Claude Boccara ESPCI ParisTech Kate Grieve
4. Intracellular dynamics measurements with full field optical coherence tomography suggest hindering effect of actomyosin contractility on organelle transport
  
  Explore OSA | OSA Publishing Oct 7 2016 Microscopy
  
  Intracellular motion can be quantitatively monitored in tissues using coherence-gated microscopic techniques. With full-field optical coherence tomography (FFOCT), the use of high numerical aperture microscope objectives provides a high resolution mapping of intracellular dynamics that are probed with subwavelength sensitivity. In the upper temporal bandwidth that we have used (1-6 Hz) the main contribution to the dynamic signal arises from the overall dynamical, optically heterogeneous cytoplasm. We propose a method to specifically study the impact of actomyosin contractility on the intracellular dynamic signal by performing high throughput, comparative measurements of multicellular aggregates with and without blebbistatin action, a selective inhibitor ...
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  Mentions: Claude Boccara ESPCI ParisTech
5. Appearance of the Retina With Full-Field Optical Coherence Tomography
  
  Explore IOVS Journal Jul 14 2016 Ophthalmology
  
  Purpose : To interpret full-field optical coherence tomography (FFOCT) images of ex vivo retina. Methods : Flatmounted retinas of human, primate, pig, sheep, rat, and mouse were imaged using FFOCT. To identify retinal ganglion and amacrine cells, fixed samples immunolabeled against Tuj1 and Brn3a or live samples labeled in vitro with green fluorescent protein (GFP) were analyzed by combining FFOCT, fluorescence confocal microscopy (FCM), and fluorescence-FFOCT. To investigate postmortem tissue changes, time series were acquired over 48 hours and on fresh versus fixed tissue. Results : With FFOCT, cell types and features such as nerve fiber bundles and RGC somas were resolved without ...
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  Mentions: Michel Pâques LLTech ESPCI ParisTech
6. Dynamic full field optical coherence tomography: subcellular metabolic contrast revealed in tissues by interferometric signals temporal analysis
  
  Explore OSA | OSA Publishing Mar 24 2016
  
  We developed a new endogenous approach to reveal subcellular metabolic contrast in fresh ex vivo tissues taking advantage of the time dependence of the full field optical coherence tomography interferometric signals. This method reveals signals linked with local activity of the endogenous scattering elements which can reveal cells where other OCT-based techniques fail or need exogenous contrast agents. We benefit from the micrometric transverse resolution of full field OCT to image intracellular features. We used this time dependence to identify different dynamics at the millisecond scale on a wide range of organs in normal or pathological conditions.
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  Mentions: Claude Boccara Fabrice Harms LLTech
7. Dynamic full field optical coherence tomography: subcellular metabolic contrast revealed in tissues by temporal analysis of interferometric signals
  
  Explore arxiv.org Jan 7 2016
  
  We developed a new endogenous approach to reveal subcellular metabolic contrast in fresh ex vivo tissues taking advantage of the time dependence of the full field optical coherence tomography interferometric signals. This method reveals signals linked with local activity of the endogenous scattering elements which can reveal cells where other imaging techniques fail or need exogenous contrast agents. We benefit from the micrometric transverse resolution of full field OCT to image intracellular features. We used this time dependence to identify different dynamics at the millisecond scale on a wide range of organs in normal or pathological conditions.
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  Mentions: Claude Boccara Fabrice Harms ESPCI ParisTech
8. Rapid evaluation of fresh ex vivo kidney tissue with full-field optical coherence tomography
  
  Explore Journal of Pathology Informatics Oct 1 2015 Oncology
  
  Background: Full-field optical coherence tomography (FFOCT) is a real-time imaging technique that rapidly generates images reminiscent of histology without any tissue processing, warranting its exploration for evaluation of ex vivo kidney tissue. Methods: Fresh tissue sections from tumor and adjacent nonneoplastic kidney ( n = 25 nephrectomy specimens; clear cell renal cell carcinoma (CCRCC) = 12, papillary RCC (PRCC) = 4, chromophobe RCC (ChRCC) = 4, papillary urothelial carcinoma (PUC) = 1, angiomyolipoma (AML) = 2 and cystic nephroma = 2) were imaged with a commercial FFOCT device. Sections were submitted for routine histopathological diagnosis. Results: Glomeruli, tubules, interstitium, and blood vessels were identified in nonneoplastic tissue. In ...
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  Mentions: Cornell University Claude Boccara LLTech
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