1. Articles from James L. Connolly

    1-8 of 8
    1. Integrated Local Binary Pattern Texture Features for Classification of Breast Tissue Imaged by Optical Coherence Microscopy

      Integrated Local Binary Pattern Texture Features for Classification of Breast Tissue Imaged by Optical Coherence Microscopy

      This paper proposes a texture analysis technique that can effectively classify different types of human breast tissue imaged by Optical Coherence Microscopy (OCM). OCM is an emerging imaging modality for rapid tissue screening and has the potential to provide high resolution microscopic images that approach those of histology. OCM images, acquired without tissue staining, however, pose unique challenges to image analysis and pattern classification. We examined multiple types of texture features and found Local Binary Pattern (LBP) features to perform better in classifying tissues imaged by OCM. In order to improve classification accuracy, we propose novel variants of LBP features ...

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    2. Swept source optical coherence microscopy using a 1310 nm VCSEL light source

      Swept source optical coherence microscopy using a 1310 nm VCSEL light source

      We demonstrate high speed, swept source optical coherence microscopy (OCM) using a MEMS tunable vertical cavity surface-emitting laser (VCSEL) light source. The light source had a sweep rate of 280 kHz, providing a bidirectional axial scan rate of 560 kHz. The sweep bandwidth was 117 nm centered at 1310 nm, corresponding to an axial resolution of 13.1 µm in air, corresponding to 8.1 µm (9.6 µm spectrally shaped) in tissue. Dispersion mismatch from different objectives was compensated numerically, enabling magnification and field of view to be easily changed. OCM images were acquired with transverse resolutions between 0 ...

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    3. Integrated Optical Coherence Tomography and Microscopy for Ex Vivo Multiscale Evaluation of Human Breast Tissues

      Integrated Optical Coherence Tomography and Microscopy for Ex Vivo Multiscale Evaluation of Human Breast Tissues

      3D tissue imaging methods are expected to improve surgical management of cancer. In this study, we examined the feasibility of two 3D imaging technologies, optical coherence tomography (OCT) and optical coherence microscopy (OCM), to view human breast specimens based on intrinsic optical contrast. Specifically, we imaged 44 ex vivo breast specimens including 34 benign and 10 malignant lesions with an integrated OCT and OCM system developed in our laboratory. The system enabled 4 um axial resolution (OCT and OCM) with 14 um (OCT) and 2 um (OCM) transverse resolution, respectively. OCT and OCM images were compared with corresponding histologic sections ...

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    4. Cellular resolution ex vivo imaging of gastrointestinal tissues with optical coherence microscopy

      Cellular resolution ex vivo imaging of gastrointestinal tissues with optical coherence microscopy

      Optical coherence microscopy (OCM) combines confocal microscopy and optical coherence tomography (OCT) to improve imaging depth and contrast, enabling cellular imaging in human tissues. We aim to investigate OCM for ex vivo imaging of upper and lower gastrointestinal tract tissues, to establish correlations between OCM imaging and histology, and to provide a baseline for future endoscopic studies. Co-registered OCM and OCT imaging were performed on fresh surgical specimens and endoscopic biopsy specimens, and images were correlated with histology. Imaging was performed at 1.06-µm wavelength with <2-µm transverse and <4-µm axial resolution for OCM, and at 14- ...

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    5. Integrated optical coherence tomography and optical coherence microscopy imaging of human pathology

      Integrated optical coherence tomography and optical coherence microscopy imaging of human pathology

      Excisional biopsy is the current gold standard for disease diagnosis; however, it requires a relatively long processing time and it may also suffer from unacceptable false negative rates due to sampling errors. Optical coherence tomography (OCT) is a promising imaging technique that provide real-time, high resolution and three-dimensional (3D) images of tissue morphology. Optical coherence microscopy (OCM) is an extension of OCT, combining both the coherence gating and the confocal gating techniques. OCM imaging achieves cellular resolution with deeper imaging depth compared to confocal microscopy. An integrated OCT/OCM imaging system can provide co-registered multiscale imaging of tissue morphology. 3D-OCT ...

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    6. Photothermal optical coherence tomography in ex vivo human breast tissues using gold nanoshells

      Photothermal optical coherence tomography in ex vivo human breast tissues using gold nanoshells

      We demonstrate photothermal optical coherence tomography (OCT) imaging in highly scattering human breast tissue ex vivo. A 120 kHz axial scan rate, swept-source phase-sensitive OCT system at 1300 nm was used to detect phase changes induced by 830 nm photothermal excitation of gold nanoshells. Localized phase modulation was observed 300-600 μm deep in scattering tissue using an excitation power of only 22 mW at modulation frequencies up to 20 kHz. This technique enables integrated structural and molecular-targeted imaging for cancer markers using nanoshells.

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    7. Ex vivo imaging of human thyroid pathology using integrated optical coherence tomography and optical coherence microscopy

      Ex vivo imaging of human thyroid pathology using integrated optical coherence tomography and optical coherence microscopy
      We evaluate the feasibility of optical coherence tomography (OCT) and optical coherence microscopy (OCM) for imaging of benign and malignant thyroid lesions ex vivo using intrinsic optical contrast. 34 thyroid gland specimens are imaged from 17 patients, covering a spectrum of pathology ranging from normal thyroid to benign disease/neoplasms (multinodular colloid goiter, Hashimoto's thyroiditis, and follicular adenoma) and malignant thyroid tumors (papillary carcinoma and medullary carcinoma). Imaging is performed using an integrated OCT and OCM system, with <4 µm axial resolution (OCT and OCM), and 14 µm (OCT) and <2 µm (OCM) transverse resolution. The system allows seamless ...
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    8. Ex vivo imaging of human pathologies with integrated optical coherence tomography (OCT) and optical coherence microscopy (OCM)

      We report an imaging study using integrated 3D-OCT and OCM for the assessment of various human thyroid and breast pathologies. The 3D-OCT data sets enable en face projection imaging, which provides large field of view with uniform focus and signal level, while OCM provides high magnification that en ... [Proc. SPIE 7168, 71680N (2009)] published Thu Feb 19, 2009.
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    1-8 of 8
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  2. Topics in the News

    1. (8 articles) James G. Fujimoto
    2. (8 articles) James L. Connolly
    3. (7 articles) Massachusetts Institute of Technology
    4. (6 articles) Beth Israel Deaconess Medical Center
    5. (6 articles) Chao Zhou
    6. (6 articles) Tsung-Han Tsai
    7. (5 articles) Aaron D. Aguirre
    8. (4 articles) Hsiang-Chieh Lee
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    Ex vivo imaging of human thyroid pathology using integrated optical coherence tomography and optical coherence microscopy Photothermal optical coherence tomography in ex vivo human breast tissues using gold nanoshells Integrated optical coherence tomography and optical coherence microscopy imaging of human pathology Cellular resolution ex vivo imaging of gastrointestinal tissues with optical coherence microscopy Integrated Optical Coherence Tomography and Microscopy for Ex Vivo Multiscale Evaluation of Human Breast Tissues Swept source optical coherence microscopy using a 1310 nm VCSEL light source Integrated Local Binary Pattern Texture Features for Classification of Breast Tissue Imaged by Optical Coherence Microscopy Evaluation of Age-related Skin Changes and Instrument Reliability Using Clinical Probe Measurements and Imaging Modalities HP-OCT™ wins Victorian AEEA award – nominated for Sir William Hudson award Postdoctoral Scholar in High Resolution Retinal Imaging at The Ohio State University Cone Photoreceptor Integrity assessed with Adaptive Optics Imaging after Laser-Pointer-Induced Retinal Injury Quantitative assessment of macular microvasculature and radial peripapillary capillary plexus in the fellow eyes of patients with retinal vein occlusion using OCT angiography