1. Articles from Lin Yang

    1-3 of 3
    1. Tracking the invasion of breast cancer cells in paper-based 3D cultures by OCT motility analysis

      Tracking the invasion of breast cancer cells in paper-based 3D cultures by OCT motility analysis

      3D paper-based cultures (PBCs) are easy-to-use and provide a biologically representative microenvironment. By stacking a sheet of cell-laden paper below sheets containing cell-free hydrogel, we form an assay capable of segmenting cells by the distance they invaded from the original cell-seeded layer. These invasion assays are limited to end-point analyses with fluorescence-based readouts due to the highly scattering nature of the paper scaffolds. Here we demonstrate that optical coherence tomography (OCT) can distinguish living cells from the surrounding extracellular matrix (ECM) or paper fibers based upon their intracellular motility amplitude ( M ). M is computed from fluctuation statistics of the sample ...

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    2. Characterizing optical coherence tomography speckle fluctuation spectra of mammary organoids during suppression of intracellular motility

      Characterizing optical coherence tomography speckle fluctuation spectra of mammary organoids during suppression of intracellular motility

      Background: An understanding of how the mammary gland responds to toxicant and drug exposures can shed light on mechanisms of breast cancer initiation/progression and therapeutic effectiveness, respectively. In this study, we employed noninvasive, label-free and high-throughput optical coherence tomography speckle fluctuation spectroscopy (OCT-SFS) to track exposure-response relationships in three-dimensional (3D) mammary epithelial organoid models. Methods: OCT-SFS is sensitive to relatively high speed (~0.16–8 µm/min) motions of subcellular light scattering components occurring over short (~2–114 s) time scales, termed “intracellular motility.” In this study, OCT speckle fluctuation spectra are quantified by two metrics: the intracellular motility ...

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    3. Depth-Resolved Imaging Based on Optical Sampling by Cavity Tuning

      Depth-Resolved Imaging Based on Optical Sampling by Cavity Tuning

      We report here a demonstration of depth-resolved optical imaging based on optical sampling by cavity tuning (OSCAT). Both optical coherence tomography and three-dimensional surface mapping are performed using an OSCAT imager. Limitations on axial resolution and pulse repetition-rate stability are discussed and viable solutions are provided. Compared with other ultrafast laser-based schemes, such as asynchronous optical sampling, OSCAT provides a simple, cost-effective solution for rapid, large-depth noninvasive imaging.

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    1-3 of 3
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    1. (2 articles) University of North Carolina
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    Depth-Resolved Imaging Based on Optical Sampling by Cavity Tuning Characterizing optical coherence tomography speckle fluctuation spectra of mammary organoids during suppression of intracellular motility Tracking the invasion of breast cancer cells in paper-based 3D cultures by OCT motility analysis Vascular Response of Orsiro vs. Xience Drug-Eluting Stents for Treating Coronary Bifurcation Lesions Trojan-Horse Diameter-Reducible Nanotheranostics for Macroscopic/Microscopic Imaging-Monitored Chemo-Antiangiogenic Therapy Prediction of Neoplastic Progression in Barrett’s Esophagus Using Nanoscale Nuclear Architecture Mapping: A Pilot Study Thickness of retinal pigment epithelium–Bruch’s membrane complex in adult Chinese using optical coherence tomography Interocular symmetry of optical coherence tomography parameters in healthy children and adolescents Fluorescein Leakage and Optical Coherence Tomography Angiography Features of Microaneurysms in Diabetic Retinopathy Cross-free in both lateral and axial directions Fourier-domain full-field optical coherence tomography Microperimetry, Humphrey field analyzer, and optical coherence tomography in detecting glaucoma: a comparative performance study Scanning optimization of an electrothermally-actuated MEMS mirror for applications in optical coherence tomography endoscopy