1. Articles from Philippe Pouliot

    1-5 of 5
    1. Fully automated dual-resolution serial optical coherence tomography aimed at diffusion MRI validation in whole mouse brains

      Fully automated dual-resolution serial optical coherence tomography aimed at diffusion MRI validation in whole mouse brains

      An automated dual-resolution serial optical coherence tomography (2R-SOCT) scanner is developed. The serial histology system combines a low-resolution (25  μm  /  voxel) 3  ×   OCT with a high-resolution (1.5  μm  /  voxel) 40  ×   OCT to acquire whole mouse brains at low resolution and to target specific regions of interest (ROIs) at high resolution. The 40  ×   ROIs positions are selected either manually by the microscope operator or using an automated ROI positioning selection algorithm. Additionally, a multimodal and multiresolution registration pipeline is developed in order to align the 2R-SOCT data onto diffusion MRI (dMRI) data acquired in the same ex vivo mouse brains ...

      Read Full Article
    2. Comparing three-dimensional serial optical coherence tomography histology to MRI imaging in the entire mouse brain

      Comparing three-dimensional serial optical coherence tomography histology to MRI imaging in the entire mouse brain

      An automated serial histology setup combining optical coherence tomography (OCT) imaging with vibratome sectioning was used to image eight wild type mouse brains. The datasets resulted in thousands of volumetric tiles resolved at a voxel size of ( 4.9 × 4.9 × 6.5 ) μ m 3 (4.9×4.9×6.5)  μm3 stitched back together to give a three-dimensional map of the brain from which a template OCT brain was obtained. To assess deformation caused by tissue sectioning, reconstruction algorithms, and fixation, OCT datasets were compared to both in vivo and ex vivo magnetic resonance imaging (MRI) imaging. The OCT ...

      Read Full Article
    3. Serial optical coherence scanning reveals an association between cardiac function and the heart architecture in the aging rodent heart

      Serial optical coherence scanning reveals an association between cardiac function and the heart architecture in the aging rodent heart

      Normal aging is accompanied by structural changes in the heart architecture. To explore this remodeling, we used a serial optical coherence tomography scanner to image entire mouse hearts at micron scale resolution. Ex vivo hearts of 7 young (4 months) and 5 old (24 months) C57BL/6 mice were acquired with the imaging platform. OCT of the myocardium revealed myofiber orientation changing linearly from the endocardium to the epicardium. In old mice, this rate of change was lower when compared to young mice while the average volume of old mice hearts was significantly larger (p<0.05). Myocardial wall thickening ...

      Read Full Article
    4. Whole mouse brain imaging using optical coherence tomography: reconstruction, normalization, segmentation, and comparison with diffusion MRI

      Whole mouse brain imaging using optical coherence tomography: reconstruction, normalization, segmentation, and comparison with diffusion MRI

      An automated massive histology setup combined with an optical coherence tomography (OCT) microscope was used to image a total of n = 5 n=5 whole mouse brains. Each acquisition generated a dataset of thousands of OCT volumetric tiles at a sampling resolution of 4.9 × 4.9 × 6.5    μ m 4.9×4.9×6.5  μm . This paper describes techniques for reconstruction and segmentation of the sliced brains. In addition to the measured OCT optical reflectivity, a single scattering photon model was used to compute the attenuation coefficients within each tissue slice. Average mouse brain templates were generated for ...

      Read Full Article
    5. Investigating the correlation between white matter and microvasculature changes in aging using large scale optical coherence tomography and confocal fluorescence imaging combined with tissue sectioning

      Investigating the correlation between white matter and microvasculature changes in aging using large scale optical coherence tomography and confocal fluorescence imaging combined with tissue sectioning

      Here, we present a serial OCT/confocal scanner for histological study of the mouse brain. Three axis linear stages combined with a sectioning vibratome allows to cut thru the entire biological tissue and to image every section at a microscopic resolution. After acquisition, each OCT volume and confocal image is re-stitched with adjacent acquisitions to obtain a reconstructed, digital volume of the imaged tissue. This imaging platform was used to investigate correlations between white matter and microvasculature changes in aging mice. Three age groups were used in this study (4, 12, 24 months). At sacrifice, mice were transcardially perfused with ...

      Read Full Article
    1-5 of 5
  1. Categories

    1. Applications:

      Art, Cardiology, Dentistry, Dermatology, Developmental Biology, Gastroenterology, Gynecology, Microscopy, NDE/NDT, Neurology, Oncology, Ophthalmology, Other Non-Medical, Otolaryngology, Pulmonology, Urology
    2. Business News:

      Acquisition, Clinical Trials, Funding, Other Business News, Partnership, Patents
    3. Technology:

      Broadband Sources, Probes, Tunable Sources
    4. Miscellaneous:

      Jobs & Studentships, Student Theses, Textbooks
  2. Topics in the News

    1. (5 articles) Polytechnique Montreal
    2. (5 articles) Frédéric Lesage
    3. (1 articles) University of Montreal
    4. (1 articles) Samsung
    5. (1 articles) Johns Hopkins University
    6. (1 articles) National Institutes of Health
    7. (1 articles) Sungkyunkwan University
    8. (1 articles) University of São Paulo
    9. (1 articles) Massachusetts Institute of Technology
    10. (1 articles) L V Prasad Eye Institute
    11. (1 articles) Gong Je Seong
    12. (1 articles) Chan Yun Kim
    13. (1 articles) James G. Fujimoto
  3. Popular Articles

  4. Picture Gallery

    Investigating the correlation between white matter and microvasculature changes in aging using large scale optical coherence tomography and confocal fluorescence imaging combined with tissue sectioning Whole mouse brain imaging using optical coherence tomography: reconstruction, normalization, segmentation, and comparison with diffusion MRI Serial optical coherence scanning reveals an association between cardiac function and the heart architecture in the aging rodent heart Comparing three-dimensional serial optical coherence tomography histology to MRI imaging in the entire mouse brain Fully automated dual-resolution serial optical coherence tomography aimed at diffusion MRI validation in whole mouse brains Factors Associated with Changes in Retinal Layers Following Acute Optic Neuritis: A Longitudinal Study Using Optical Coherence Tomography Relationship between N95 Amplitude of Pattern Electroretinogram and Optical Coherence Tomography Angiography in Open-Angle Glaucoma Historic Effort to 'End Blindness" Marks a Major Milestone with Leading Scientist Recognized for Pioneering Work at VIP Ceremony Featuring Tribute to Ruth Bader Ginsburg Healing and early stent coverage after ultrathin strut biodegradable polymer‐coated sirolimus‐eluting stent implantation: SiBi optical coherence tomography study Influences of hypomagnesemia on optic nerve and retinal vascular structure determined using optical coherence tomography angiography Repeatability and agreement of total corneal and sublayer pachymetry with 2 different algorithms of Fourier-domain optical coherence tomography in myopic and postphotorefractive keratectomy eyes Vessel Density around Foveal Avascular Zone as a Potential Imaging Biomarker for Detecting Preclinical Diabetic Retinopathy: An Optical Coherence Tomography Angiography Study