1. Articles from Philippe Pouliot

    1-5 of 5
    1. Fully automated dual-resolution serial optical coherence tomography aimed at diffusion MRI validation in whole mouse brains

      Fully automated dual-resolution serial optical coherence tomography aimed at diffusion MRI validation in whole mouse brains

      An automated dual-resolution serial optical coherence tomography (2R-SOCT) scanner is developed. The serial histology system combines a low-resolution (25  μm  /  voxel) 3  ×   OCT with a high-resolution (1.5  μm  /  voxel) 40  ×   OCT to acquire whole mouse brains at low resolution and to target specific regions of interest (ROIs) at high resolution. The 40  ×   ROIs positions are selected either manually by the microscope operator or using an automated ROI positioning selection algorithm. Additionally, a multimodal and multiresolution registration pipeline is developed in order to align the 2R-SOCT data onto diffusion MRI (dMRI) data acquired in the same ex vivo mouse brains ...

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    2. Comparing three-dimensional serial optical coherence tomography histology to MRI imaging in the entire mouse brain

      Comparing three-dimensional serial optical coherence tomography histology to MRI imaging in the entire mouse brain

      An automated serial histology setup combining optical coherence tomography (OCT) imaging with vibratome sectioning was used to image eight wild type mouse brains. The datasets resulted in thousands of volumetric tiles resolved at a voxel size of ( 4.9 × 4.9 × 6.5 ) μ m 3 (4.9×4.9×6.5)  μm3 stitched back together to give a three-dimensional map of the brain from which a template OCT brain was obtained. To assess deformation caused by tissue sectioning, reconstruction algorithms, and fixation, OCT datasets were compared to both in vivo and ex vivo magnetic resonance imaging (MRI) imaging. The OCT ...

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    3. Serial optical coherence scanning reveals an association between cardiac function and the heart architecture in the aging rodent heart

      Serial optical coherence scanning reveals an association between cardiac function and the heart architecture in the aging rodent heart

      Normal aging is accompanied by structural changes in the heart architecture. To explore this remodeling, we used a serial optical coherence tomography scanner to image entire mouse hearts at micron scale resolution. Ex vivo hearts of 7 young (4 months) and 5 old (24 months) C57BL/6 mice were acquired with the imaging platform. OCT of the myocardium revealed myofiber orientation changing linearly from the endocardium to the epicardium. In old mice, this rate of change was lower when compared to young mice while the average volume of old mice hearts was significantly larger (p<0.05). Myocardial wall thickening ...

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    4. Whole mouse brain imaging using optical coherence tomography: reconstruction, normalization, segmentation, and comparison with diffusion MRI

      Whole mouse brain imaging using optical coherence tomography: reconstruction, normalization, segmentation, and comparison with diffusion MRI

      An automated massive histology setup combined with an optical coherence tomography (OCT) microscope was used to image a total of n = 5 n=5 whole mouse brains. Each acquisition generated a dataset of thousands of OCT volumetric tiles at a sampling resolution of 4.9 × 4.9 × 6.5    μ m 4.9×4.9×6.5  μm . This paper describes techniques for reconstruction and segmentation of the sliced brains. In addition to the measured OCT optical reflectivity, a single scattering photon model was used to compute the attenuation coefficients within each tissue slice. Average mouse brain templates were generated for ...

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    5. Investigating the correlation between white matter and microvasculature changes in aging using large scale optical coherence tomography and confocal fluorescence imaging combined with tissue sectioning

      Investigating the correlation between white matter and microvasculature changes in aging using large scale optical coherence tomography and confocal fluorescence imaging combined with tissue sectioning

      Here, we present a serial OCT/confocal scanner for histological study of the mouse brain. Three axis linear stages combined with a sectioning vibratome allows to cut thru the entire biological tissue and to image every section at a microscopic resolution. After acquisition, each OCT volume and confocal image is re-stitched with adjacent acquisitions to obtain a reconstructed, digital volume of the imaged tissue. This imaging platform was used to investigate correlations between white matter and microvasculature changes in aging mice. Three age groups were used in this study (4, 12, 24 months). At sacrifice, mice were transcardially perfused with ...

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    1-5 of 5
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  2. Topics in the News

    1. (5 articles) Polytechnique Montreal
    2. (5 articles) Frédéric Lesage
    3. (1 articles) University of Montreal
    4. (1 articles) University of Illinois at Urbana-Champaign
    5. (1 articles) Stephen A. Boppart
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    Investigating the correlation between white matter and microvasculature changes in aging using large scale optical coherence tomography and confocal fluorescence imaging combined with tissue sectioning Whole mouse brain imaging using optical coherence tomography: reconstruction, normalization, segmentation, and comparison with diffusion MRI Serial optical coherence scanning reveals an association between cardiac function and the heart architecture in the aging rodent heart Comparing three-dimensional serial optical coherence tomography histology to MRI imaging in the entire mouse brain Fully automated dual-resolution serial optical coherence tomography aimed at diffusion MRI validation in whole mouse brains Longitudinal optical coherence tomography to visualize the in vivo response of middle ear biofilms to antibiotic therapy Verification of cascade optical coherence tomography for freeform optics form metrology - Osa Swept Source Optical Coherence Tomography Analysis of the Selected Eye’s Anterior Segment Parameters Observation of fundus and optical coherence tomography angiography manifestations in carotid cavernous fistula patients Optimizing 3D retinal vasculature imaging in diabetic retinopathy using registration and averaging of OCT-A Accurately motion-corrected Lissajous OCT with multi-type image registration Long-term in vivo monitoring of gliotic sheathing of ultrathin entropic coated brain microprobes with fiber-based optical coherence tomography