1. Dynamic full-field optical coherence tomography: 3-D live-imaging of retinal organoids

    Dynamic full-field optical coherence tomography: 3-D live-imaging of retinal organoids

    Optical coherence tomography offers astounding opportunities to image the complex structure of living tissue but lacks functional information. We present dynamic full-field optical coherence tomography as a technique to noninvasively image living human induced pluripotent stem cell (hiPSC)-derived retinal organoids. Colored images with an endogenous contrast linked to organelle motility are generated, with submicrometer spatial resolution and millisecond temporal resolution, creating a way to identify specific cell types in living tissue via their dynamic profile.

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