1. Visualization of 3D cell migration using high speed ultrahigh resolution optical coherence tomography

    Visualization of 3D cell migration using high speed ultrahigh resolution optical coherence tomography

    Using high speed ultrahigh resolution optical coherence tomography (OCT) at 800nm, non-invasive 3D cellular imaging has been accomplished. Cellular resolution imaging on and within these types of substrates is not possible with conventional microscopy techniques such as interference contrast microscopy, and requires the use of fluorescent staining. It is possible to achieve data acquisition rates of 20,000 samples per second with OCT which, in combination with its high axial and transverse resolution (>2-3µm), allows it to be used as a non-invasive technique to analyze cell migration in 3D with time. Comparatively high penetration depth also makes OCT a ...

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