1. Histologic Correlation of Pig Retina Radial Stratification with Ultrahigh-Resolution Optical Coherence Tomography

    Histologic Correlation of Pig Retina Radial Stratification with Ultrahigh-Resolution Optical Coherence Tomography

    purpose. To compare ultrahigh-resolution optical coherence tomography (OCT) cross-sectional images of the pig retina with histology, to evaluate the potential of ultrahigh-resolution OCT for enhanced visualization of intra- and subretinal structures. methods. Ultrahigh-resolution OCT images were acquired with 1.4-μm axial × 3-μm transverse resolution from in vitro posterior eyecup preparations of the domestic pig. Frozen sections were obtained in precise alignment with OCT tomograms, by using major blood vessels as orientation markers and were counterstained with cresyl violet or unstained and examined by differential interference contrast microscopy. Micrographs from histologic sections were linearly scaled to correct for tissue ...

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