Characterizing optical coherence tomography speckle fluctuation spectra of mammary organoids during suppression of intracellular motility
Background: An understanding of how the mammary gland responds to toxicant and drug exposures can shed light on mechanisms of breast cancer initiation/progression and therapeutic effectiveness, respectively. In this study, we employed noninvasive, label-free and high-throughput optical coherence tomography speckle fluctuation spectroscopy (OCT-SFS) to track exposure-response relationships in three-dimensional (3D) mammary epithelial organoid models. Methods: OCT-SFS is sensitive to relatively high speed (~0.16–8 µm/min) motions of subcellular light scattering components occurring over short (~2–114 s) time scales, termed “intracellular motility.” In this study, OCT speckle fluctuation spectra are quantified by two metrics: the intracellular motility ...
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