1. Redox imaging and optical coherence tomography of the respiratory ciliated epithelium

    Redox imaging and optical coherence tomography of the respiratory ciliated epithelium

    Optical coherence tomography (OCT) is an emerging technology for in vivo< airway and lung imaging. However, OCT lacks sensitivity to the metabolic changes caused by inflammation, which drives chronic respiratory diseases such as asthma and chronic obstructive pulmonary disorder. Redox imaging (RI) is a label-free technique that uses the autofluorescence of the metabolic coenzymes NAD(P)H and flavin adenine dinucleotide (FAD) to probe cellular metabolism and could provide complimentary information to OCT for airway and lung imaging. We demonstrate OCT and RI of respiratory ciliated epithelial function in ex vivo mouse tracheae. We applied RI to measure cellular metabolism ...

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