1. OCT-Angiography for Non-Invasive Monitoring of Neuronal and Vascular Structure in Mouse Retina: Implication for Characterization of Retinal Neurovascular Coupling

    OCT-Angiography for Non-Invasive Monitoring of Neuronal and Vascular Structure in Mouse Retina: Implication for Characterization of Retinal Neurovascular Coupling

    Purpose: Optical coherence tomography angiography (OCT-A) is a newly developed technique to visualize retinal vasculature noninvasively based on interferometry. Although OCT-A has been used clinically, its applications in small animal studies have been limited. This study is designed to develop and demonstrate the feasibility of a protocol for the use of an en-face OCT-based method to visualize and quantify retinal microvasculature in mice that can be used for in vivo assessment of retina ischemia. Methods: A customized algorithm was developed to extract angiographic profiles of the mouse retina from en-face OCT using an unmodified Bioptigen Envisu R-Class OCT imaging system ...

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