1. Appearance of the Retina With Full-Field Optical Coherence Tomography

    Appearance of the Retina With Full-Field Optical Coherence Tomography

    Purpose : To interpret full-field optical coherence tomography (FFOCT) images of ex vivo retina. Methods : Flatmounted retinas of human, primate, pig, sheep, rat, and mouse were imaged using FFOCT. To identify retinal ganglion and amacrine cells, fixed samples immunolabeled against Tuj1 and Brn3a or live samples labeled in vitro with green fluorescent protein (GFP) were analyzed by combining FFOCT, fluorescence confocal microscopy (FCM), and fluorescence-FFOCT. To investigate postmortem tissue changes, time series were acquired over 48 hours and on fresh versus fixed tissue. Results : With FFOCT, cell types and features such as nerve fiber bundles and RGC somas were resolved without ...

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